• Animal cells and systems
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• v.4 no.1
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• pp.77-85
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• 2000

This study has demonstrated the molecular variation of 5S rRNA genes in 15 Allium subgenus Rhizirideum and 1 Allium subg. Allium. For cloning of the 5S rRNA genes, PCR products were obtained from amplification with oligonucleotide primers which were derived from the conserved coding region of 5S rRNA genes. These amplified PCR products were cloned and identified by FISH and sequence analysis. The 5S rRNA loci were primarily located on chromosomes 5 and/or 7 in diploid species and various chromosomes in alloploid species. The size of the coding region of 5S rRNA genes was 120 bp in all the species and the sequences were highly conserved within Allium species. The sizes of nontranscribed spacer (NTS) region were varied from 194 bp (A. dektiude-fustykisum, 2n=16) to 483 bp (A. sativum). Two kinds of NTS regions were observed in A. victorialis var. platyphyllum a diploid, A. wakegi an amphihaploid, A. sacculiferum, A. grayi, A. deltoide-fistulosum and A. wenescens all allotetraploids, while most diploid species showed only one NTS region. The species containing two components of NTS region were grouped with different diploid species in a phylogenetic tree analysis using the sequences of 5S rRNA genes and adjacent non-coding regions.

• Microbiology and Biotechnology Letters
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• v.46 no.3
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• pp.288-299
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• 2018

Food bio preservation is of major interest in the food industry. Many types of antimicrobial compounds can be produced by lactic acid bacteria (LAB), including bacteriocins. Bacteriocins increase the shelf-life of food by decreasing some food-borne diseases. In this study, a multi-coding sequence of bacteriocin genes was used for primer design to produce bacteriocin genes in Enterococcus faecium AH2 strain and Pediococcus pentosaceus AH1. Multi-coding sequences were aligned to detect conserved sequences in the bacteriocin gene. Eight genes encoding proteins involved in bacteriocin production were isolated and sequenced, including six from E. faecium AH2 (entA, entI, entF, entR, orfA2, orfA3) and two from P. pentoceseus AH1 (papA, pedB), and all gene sequences were deposited in the Gen Bank database under accession numbers LC064146-LC064151, LC101300, and LC101789, respectively. P. pentosaceus AH1 and E. faecium AH2 strains displayed bacteriocin activities of $2610AU\;mL^{-1}$ and $690AU\;mL^{-1}$ and inhibition zones of 26 mm and 19 mm, respectively. Overexpression of entA in E. faecium AH2 increased the bacteriocin and antimicrobial activities.

• Journal of Microbiology
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• v.37 no.2
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• pp.102-110
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• 1999

Genetic determinant for the secondary metabolism was studied in heterologous expression in Streptomyces lividans TK-24 using Streptomyces griseus ATCC 10137 as a donor strain. Chromosomal DNA of S. griseus was ligated into the high-copy number Streptomyces shuttle plasmid, pWHM3, and introduced into S. lividans TK-24. A plasmid clone with 4.3-kb BamHI DNA of S. griseus (pMJJ201) was isolated by detecting for stimulatory effect on actinorhodin production by visual inspection. The 4.3-kb BamHI DNA was cloned into pWHM3 under the control of the strong constitutive ermEp promoter in both directions (pMJJ202); ermEp promoter-mediated transcription for coding sequence reading right to left: pMJJ203; ermEp promoter-mediated transcription for coding sequence reading left to right) and reintroduced into S. lividans TK-24. The production of actinorhodin was markedly stimulated due to introduction of pMJJ202 on regeneration agar. The introduction of pMJJ202 also stimulated production of actinorhodin and undecylproidigiosin in submerged culture employing the actinorhodin production medium. Introduction of pMJJ203 resulted in a marked decrease of production of the two pigments. Nucleotide sequence analysis of the 4.3-kb region revealed three coding sequences: two coding sequences reading left to right, ORF1 and ORF2, one coding sequence reading right to left, ORF3. Therefore, it was suggested that the ORF3 product was responsible for the stimulation of antibiotic production. The C-terminal region of ORF3 product showed a local alignment with Myb-related transcriptional factors, which implicated that the ORF3 product might be a novel DNA-binding protein related to the regulation of secondary metabolism in Streptomyces.

• Journal of Broadcast Engineering
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• v.15 no.3
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• pp.434-453
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• 2010

In this paper, we propose 3DTE(3-Dimensional Transform Environment) that is based on 3DT(Dimensional Transform) that performs 2-dimensional integer DCT(Discrete Cosine Transform) based on $4{\times}4$ block and 1-dimensional integer DCT based on $4{\times}1$ block after collecting same frequency coefficients in neighboring $4{\times}4$ block and supports it's additional coding tools for high performance. The transform of 3DT can keep prediction error by using $4{\times}4$ block and reduce spatial redundancy additionally. The proposed 3DTE can provide coding tools to improve the coding efficiency with using 3DT. The performance of 3DTE compared to JM11.0 is average 3.58% and 5.40% bit savings for all test sequences and HD sequences, respectively, with keeping subjective video quality in High profile.

• Journal of Broadcast Engineering
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• v.9 no.3
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• pp.236-245
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• 2004

A multiview sequence CODEC with view scaiability is proposed in this paper. We define a GGOP (Group of GOP) structure as a basic coding unit to efficiently code multiview sequences. 7he proposed CODEC provides flexible GGOP structures based on the number of views and baseline distances among cameras. Multiview sequences encode consists of disparity estimation/compensation, motion estimation/compensation, residual coding and rate control and generates multiview sequence bitstream. The main bitstream is the same as an MPEG-2 mono-sequence bitstream for MPEG-2 compatibility. The auxiliary bitstream contains information concerning the remaining multiview sequences except for the reference sequences. The proposed CODEC with view scalability provides that a number of view flints are selectively determined at the receiver according to the type of display modes. The proposed multiview sequence CODEC is tested with several multiview sequences to determine its flexibility. compatibility with MPEG-2 and view scaiability. In addition, we subjectively confirm that the decoded bitstreams with view scaiability can be Properly displayed by several types of display modes. including 3D monitors.

• Korean Journal of Microbiology
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• v.25 no.2
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• pp.94-102
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• 1987

Rat liver LDH A-cDNA has been isolated from a .lambda.gt11-rat lover cDNA library and partially characterized. The size of the isolated rat liver LDH A-cDNA if shown to be 1.6Kb and restriction enzyme sites for the rat liver LDH A-cDNA are also mapped. 682-nucleotide sequence coding for 3'-end of rat liver LDH A-cDNA has been analyzed and compared to the nucleotide sequence of the same region of rat $C_6$-glioma cell LDH A-cDNA which has been cloned from the hormonally stimulated cell cultures. The result shows that 177 nucleotide sequences coding for the C-terminal 59-amino acids are identical but 505 nucleotide sequences of 3'-nontranslated region of the two LSH A-cDNA exhibit characteristic differences in thier nucleotide sequences. Computer analysis for the folding structures for 3'-end 400 nucleotide sequences of the two LDH A-cDNA shows a possibility implying that the two LDH A-mRNAs isolated from different tissues of rats may have different half life and therefore their translational efficiency may be different. It has been previously demonstrated that isoproterenol stimulated rat $C_6$ -glioma cell cultures produce LDH A-mRNA showing 2 to 3-fold longer half life in comparison to that of noninduced LHD A-mRNA. The result therefore support for the idea that hormonally stimulated rat $C_6$-glioma cells may produce LDH A-mRNA containing different nucleotide sequences at the 3'-end nontranslated region by which the hormonally induced LDH A-mRNA could have more stable secondary mRAN structure in comparison to that of noninduced LDH A-mRNA.

• Proceedings of the IEEK Conference
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• 2000.06d
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• pp.92-97
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• 2000

An AMR(Adaptive Multi-Rate) speech coding algorithm has been adopted as a standard speech codec for IMT-2000. It is based on the algebraic CELP, and consists of eight speech coding modes having the bit rate from 4.75 kbit/s to 12.2 kbit/s. It also contains the VAD(Voice Activity Detector), SCR (Source Controlled Rate) operation, and error concealment scheme for robustness in a radio channel. The bit rate of AMR is changed on a frame basis depending on the channel condition. In this paper, we introduced AMR speech coding algorithm and performed the real-time implementation using TMS320C6201, i.e., a Texas Instrument's fixed-point DSP. With the ANSI C source code released from ETSI and 3GPP, we convert and optimize the program to make it run in real time using the C compiler and assembly language. It is verified that the decoded result of the implemented speech codec on the DSP is identical with the PC simulation result using ANSI C code for test sequences. Also, actual sound input/output test using microphone and speaker demonstrates its proper real-time operation without distortions or delays.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.4
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• pp.834-847
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• 1996

A selective coding scheme is proposed that describes a method for coding image sequences distinguishing bits between background and target region. The suggested method initially estimates global motion parameters and local motion vectors. Then segmentation is performed with a hierarchical clustering scheme and a quadtree algorithm in order to divide the processing image into the backgraound and target region. Finally image coding is done by assigning more bits to the target region and less bits to background so that the target region may be reconstructed with high quality. Simulations show that the suggested algorithm performs well especially in the circumstances where background changes and target regionis small enough compared with that of background.

• ETRI Journal
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• v.29 no.1
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• pp.18-26
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• 2007

This paper presents an improved redundant picture coding method that efficiently enhances the error resiliency of H.264. The proposed method applies polyphase downsampling to residual blocks obtained from inter prediction and selectively encodes the rearranged residual blocks in the redundant picture coding process. Moreover, a spatial-temporal sample construction method is developed for the redundant coded picture, which further improves the reconstructed picture quality in error prone environments. Simulations based on JM11.0 were run to verify the proposed method on different test sequences in various error prone environments with average packet loss rates of 3%, 5%, 10%, and 20%. Results of the simulations show that the presented method significantly improves the robustness of H.264 to packet loss by 1.6 dB PSNR on average over the conventional redundant picture coding method.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.31B no.8
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• pp.87-98
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• 1994

Object-oriented analysis-synthesis coding subdivides each image of a sequence into moving objects and compensates the motion of each object. Thus it can reconstruct real motion better than conventional motion-compensated coding techniques at very-low-bit-rates. It uses a mapping parameter technique for estimating motion information of each object. Since a mapping parameter technique uses gradient operators it is sensitive to redundant details and noise. To accurately determine mapping parameters, we propose a new analysis method using integral projections for estimation of gradient values. Also to reconstruct correctly the local motion the proposed algorithm divides an image into segmented objects each of which having uniform motion information while the conventional one assumes a large object having the same motion information. Computer simulation results with several test sequences show that the proposed image analysis method in object-oriented analysis-synthesis coding shows better performance than the conventional one.