• Biotechnology and Bioprocess Engineering:BBE
• /
• v.11 no.6
• /
• pp.510-515
• /
• 2006

The putative EPA synthesis gene cluster was mined from the entire genome sequence of Shewanella oneidensis MR-1. The gene cluster encodes a PKS-like pathway that consists of six open reading frames (ORFs): ORFSO1602 (multi-domain beta-ketoacyl synthase, KS-MAT-4ACPs-KR), ORFSO1600 (acyl transferase, AT), ORFSO1599 (multi-domain beta-ketoacyl synthase, KS-CLF-DH-DH), ORFSO1597 (enoyl reductase, ER), ORFSO1604 (phosphopentetheine transferase, PPT), and ORFSO1603 (transcriptional regulator). In order to prove involvement of the PKS-like machinery in EPA synthesis, a 20.195-kb DNA fragment containing the genes was amplified from S. oneidensis MR-1 by the long-PCR method. Its identity was confirmed by the methods of restriction enzyme site mapping and nested PCR of internal genes orfSO1597 and orfSO1604. The DNA fragment was cloned into Escherichia coli using cosmid vector SuperCos1 to form pCosEPA. Synthesis of EPA was observed in four E. coli clones harboring pCosEPA, of which the maximum yield was 0.689% of the total fatty acids in a clone designated 9704-23. The production yield of EPA in the E. coli clone was affected by cultivation temperature, showing maximum yield at $20^{\circ}C$ and no production at $30^{\circ}C$ or higher. In addition, production yield was inversely proportional to glucose concentration of the cultivation medium. From the above results, it was concluded that the PKS-like modules catalyze the synthesis of EPA. The synthetic process appears to be subject to regulatory mechanisms triggered by various environmental factors. This most likely occurs via the control of gene expression, protein stability, or enzyme activity.

• Journal of Korean Academy of Nursing Administration
• /
• v.20 no.4
• /
• pp.394-404
• /
• 2014

Purpose: The purpose of this study was to identify the conceptual structure of stressors experienced by nursing students during clinical practice. Methods: Ten men and 10 women nursing college students were interviewed. The results were 208 ideas. By synthesizing and editing these ideas, the final statement was trimmed down to 39 questions. The next step was to have the participants classify these 39 final questions. They were asked to rate stress scores using a five-point scale. Through multidimensional scaling analysis and hierarchical cluster analysis yielded dimensions and clusters. Results: Results of the study showed 2 dimensions which were classified as 'interpersonal relationship-practice system' and 'identity-practice environment'. It also yielded 8 clusters which were classified as 'role confusion', 'gender discrimination', 'attitudes of medical personnel and patients', 'comparison between fellow students', 'difference between theory and practice', 'disestablishing the role of practice guide', 'interference with training', and 'problems of the practice environment'. Further, stress factors and stress levels were differentiated depending on the gender of the student. Conclusion: The results of the study indicate that educators who are developing clinical practice programs and clinical practitioners should consider stressors during clinical practice and the educational implications to nursing students.

• Journal of Sensor Science and Technology
• /
• v.25 no.1
• /
• pp.13-19
• /
• 2016

Nowadays, most major automobile manufacturers are very interested, and actively involved, in developing driver alcohol detection system for safety (DADSS) that serves to prevent driving under the influence. DADSS measures the blood alcohol concentration (BAC) from the driver's breath and limits the ignition of the engine of the vehicle if the BAC exceeds the reference value. In this study, to optimize the sensor array of the DADSS, we selected sensors by using three different methods, configured the sensor arrays, and then compared their performance. The Wilks' lambda, stepwise elimination and filter method (using a principal component) were used as the sensor selection methods [2,3]. We compared the performance of the arrays, by using the selectivity and sensitivity as criteria, and Sammon mapping for the analysis of the cluster type of each gas. The sensor array configured by using the stepwise elimination method exhibited the highest sensitivity and selectivity and yielded the best visual result after Sammon mapping.

• Korean Journal of Childcare and Education
• /
• v.10 no.5
• /
• pp.191-209
• /
• 2014

This study aims at figuring out perception of teachers on the education of a child's personality development using concept mapping and demonstrating its importance. To recognize concept mapping of teacher's perception on the education of a child's personality development, the procedure of the research are as follows. The specific research results are as follows: First, based on the categorization, using multidimensional scaling and hierarchical cluster analysis, the results were concept mapping with 2-dimensions and five clusters. Second, dimensions of perception of teachers on the education of a child's personality development in concept mapping, showed virtue in left and performance in right, respectively, in X-axis, while environmental factor was in the upper area and personal factor was in the lower area, in Y-axis. Third, teachers' perception on the importance on education of child's personality development was evaluated in 5 categories as follows: 'Emphasizing adult's role'(M=4.45) was perceived as the most important among categories related to the education of a child's personality development. These results can be used for developing teacher's training program or an institutional supporting plan to raise performance of the education of a child's personality development in the future.

• Journal of KIISE:Databases
• /
• v.31 no.2
• /
• pp.108-121
• /
• 2004

In this paper, we propose an enhanced snapshot technique that solves performance degradation when snapshot is initiated for the storage cluster system. However, traditional snapshot technique has some limits adapted to large amount storage shared by multi-hosts in the following aspects. As volume size grows, (1) it deteriorates crucially the performance of write operations due to additional disk access to verify COW is performed. (2) Also it increases excessively the blocking time of write operation performed during the snapshot creation time. (3)Finally, it deteriorates the performance of write operations due to additional disk I/O for mapping block caused by the verification of COW. In this paper, we propose an efficient snapshot technique for large amount storage shared by multi-hosts in SAN Environments. We eliminate the blocking time of write operation caused by freezing while a snapshot creation is performing. Also to improve the performance of write operation when snapshot is taken, we introduce First Allocation Bit(FAB) and Snapshot Status Bit(SSB). It improves performance of write operation by reducing an additional disk access to volume disk for getting snapshot mapping block. We design and implement an efficient snapshot technique, while the snapshot deletion time, improve performance by deallocation of COW data block using SSB of original mapping entry without snapshot mapping entry obtained mapping block read from the shared disk.

• Journal of Gifted/Talented Education
• /
• v.25 no.2
• /
• pp.321-337
• /
• 2015

The purpose of the present study is to examine elementary gifted students' perceptions on bias against creativity utilizing concept mapping approach. Twelve elementary gifted students participated in the group brainstorming and produced 55 final statements. Based on these statements, the multi-dimensional scale and hierarchial cluster analysis using dissimilarity matrix were performed. Average stress value was .30 which is appropriate for a two-dimensional concept mapping study. In addition, a questionnaire survey using likert 6 points scale was carried out targeting 132 elementary gifted students to analyze the degree of sympathy on their anti-creativity bias perception. The findings are as follow: First, four categories were concluded dividing gifted students' perceptions on bias against creativity from the hierarchial cluster analysis with X-Y coordinate matrix, these were 'Contradictory attitudes to creativity', 'Low evaluation for creativity', 'Forced to predetermined rules and ideas', and 'Aversion to new things'. Second, elementary gifted students were sympathetic to the order 'Forced to predetermined rules and ideas'(M=4.16), 'Aversion to new things'(M=3.68), 'Contradictory attitudes to creativity'(M=3.55) and 'Low evaluation for creativity'(M=3.30). This study aims to examine, analyze and categorize various relevant factors related to elementary gifted students' perceptions on bias against creativity. Implications of the study related to the present and future creative education were discussed in depth.

• Science of Emotion and Sensibility
• /
• v.19 no.4
• /
• pp.55-70
• /
• 2016

It has been suggested that few studies were completed on psychological aspect of coffee drinking behavior though coffee researches have been mainly focused on marketing or business purposes. In this study, fifteen university students were participated to a group interview for concept mapping analysis asking about their meaning and purpose of coffee drinking behavior. Sixty nine statements were extracted from the interview, and categorized into seven clusters using multi dimensional scaling analysis and hierarchical cluster analysis. Seven clusters were Personal method of getting the desired physical effect, As a method of social activities, Method to get psychological consolation, Drink for spending time and using space, Habitual drink and use of caffeine's effect, Enjoying coffee's various characteristic and attraction, and Enjoying coffee's various characteristic and attraction. Two factors were identified based on these clusters such as internal-external motivation to drink coffee and emotional-physical effect of coffee. Participants ranked their priority of those clusters; the cluster of "Drink for spending time and using space" was first ranked. Limitations and suggestions for future research were also discussed.

• Publications of The Korean Astronomical Society
• /
• v.27 no.4
• /
• pp.221-222
• /
• 2012

We present the results of far-infrared spectral mapping of the Galactic center region with FIS-FTS, which covered the two massive star-forming clusters, Arches and Quintuplet. We find that two dust components with temperatures of about 20 K and 50 K are required to fit the overall continuum spectra. The warm dust emission is spatially correlated with the [OIII] $88{\mu}m$ emission and both are likely to be associated with the two clusters, while the cool dust emission is more widely distributed without any clear spatial correlation with the clusters. We find differences in the properties of the ISM around the two clusters, suggesting that the star-forming activity of the Arches cluster is at an earlier stage than that of the Quintuplet cluster.

• Journal of Plant Biotechnology
• /
• v.44 no.4
• /
• pp.349-355
• /
• 2017

The amino acid composition of rice is a major concern of rice breeders because amino acids are among the most important nutrient components in rice. In this study, a genetic map was constructed with a population of 134 recombinant inbred lines (RILs) from a cross between Dasanbyeo (Tongil-type indica) and TR22183 (temperate japonica), as a means to detect the main and epistatic effect quantitative trait loci (QTLs) for the amino acid content (AAC). Using a linkage map which covered a total of 1458 cM based on 239 molecular marker loci, a total of six main-effect QTLs (M-QTLs) was identified for the content of six amino acids that were mapped onto chromosome 3. For all the M-QTLs, the TR22183 allele increased the trait values. The QTL cluster (flanked by id3015453 and id3016090) on chromosome 3 was associated with the content of five amino acids. The phenotypic variation, explained by the individual QTLs located in this cluster, ranged from 10.2 to 12.4%. In addition, 26 epistatic QTLs (Ep-QTLs) were detected and the 25 loci involved in this interaction were distributed on all nine chromosomes. Both the M-QTLs and Ep-QTLs detected in this study will be useful in breeding programs which target the development of rice with improved amino acid composition.

• Journal of the Korean Society for Precision Engineering
• /
• v.29 no.5
• /
• pp.500-505
• /
• 2012

Recently, a high speed spindle is an essential part of machine tools to satisfy latest demand of high precision product and machining of hard materials. But, there are many disadvantages such as heat generation of built-in-motor, bearing friction, noise, vibration and displacement because of the high speed. Many researches on spindle systems have been conducted for solving these problems. In this study, technical trend of machine tools spindle systems are analyzed with patent PSM, mapping and grouping. The analysis is carried out for the applied patent during January 2000 and December 2009 in Korea, Japan, EU and U.S.A. And development of the direction, strategy and promising technologies of the spindle system are suggested.