• Korean journal of applied entomology
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• v.58 no.2
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• pp.159-164
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• 2019

The purpose of this study was to investigate whether leaf damage in pear orchards caused by large black chafer beetles can be controlled through net traps and clove oil treatment. In June 2017, we measured large black chafer beetle population density and leaf damage rates in two orchards, located in Boseong and Hampyeong, under three treatments: net, net plus clove oil, and control. In order to evaluate the effect of net position on the adult beetles, nets were placed outside the orchard and along the upper and lower parts of a horizontal trellis. The adult beetle population density was 20.4 to 34.7% lower in the net alone treatment, and 21.1 to 38.1% lower in the combined net and clove oil treatment than in the control. The adult beetle population density was 10.9-14.4 times higher outside the orchard and 5.1 to 9.1 times higher in the upper parts of the horizontal trellis than in the lower parts of the horizontal trellis. Leaf damage under both the net only and net plus clove oil treatments was significantly lower than that in the control at both sites. Therefore, intensive net installation around orchards may be a practical organic alternative to prevent leaf damage caused by large black chafer beetles.

• Culinary science and hospitality research
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• v.5 no.2
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• pp.401-418
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• 1999

Many plants and animal have long been known to have medicinal effects and therefore have been used as medicines. There are many substances that show various pharmacologic efficacy such as anti-tumor efficacy, anti-inflammatory efficacy, cholesterol-lowering efficacy, anti-coagulant of blood efficacy and anti-bacterial efficacy. I summarized the recent advances in research on physiologically functional foods. The pharmacological efficacy of dietary fiber, chitin & chitosan, DHA(docosahexaenoic acid), mushroom, alginic acid and herbs have selected as topices for discussion. I was examining the anti-coagulant activity of herbs, I discovered that Eugenia caryophyllata T. (clove) had a relatively high anti-coagulant activity.

• Microbiology and Biotechnology Letters
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• v.48 no.1
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• pp.12-23
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• 2020

Edible films containing antimicrobial agents can be used as safe alternatives to preserve food products. Essential oils are well-recognized antimicrobials. However, their low water solubility, volatility and high sensitivity to oxygen and light limit their application in food preservation. These limitations could be overcome by embedding these essential oils in complexed product matrices exploiting the encapsulation efficiency of β-cyclodextrin. This study focused on the maximization of β-cyclodextrin production using cyclodextrin glucanotransferase (CGTase) and the evaluation of its encapsulation efficacy to fabricate edible antimicrobial films. Response surface methodology (RSM) was used to optimize CGTase production by Brevibacillus brevis AMI-2 isolated from mangrove sediments. This enzyme was partially purified using a starch adsorption method and entrapped in calcium alginate. Cyclodextrin produced by the immobilized enzyme was then confirmed using high performance thin layer chromatography, and its encapsulation efficiency was investigated. The clove oil/β-cyclodextrin inclusion complexes were prepared using the coprecipitation method, and incorporated into chitosan films, and subjected to antimicrobial testing. Results revealed that β-cyclodextrin was produced as a major product of the enzymatic reaction. In addition, the incorporation of clove oil/β-cyclodextrin inclusion complexes significantly increased the antimicrobial activity of chitosan films against Staphylococcus aureus, Staphylococcus epidermidis, Salmonella Typhimurium, Escherichia coli, and Candida albicans. In conclusion, B. brevis AMI-2 is a promising source for CGTase to synthesize β-cyclodextrin with considerable encapsulation efficiency. Further, the obtained results suggest that chitosan films containing clove oils encapsulated in β-cyclodextrin could serve as edible antimicrobial food-packaging materials to combat microbial contamination.

• Journal of the Korean Wood Science and Technology
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• v.42 no.1
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• pp.41-48
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• 2014

To development of the low toxic preservatives, we prepared 29 kinds of chemicals using dinotefuran of low toxic insecticide, IPBC and propiconazol of fungicide, and clove oil has fungicidal activity, but is harmless to humans. It was conducted to investigate the inhibitory effectiveness of mycelial growth and anti-mold effectiveness to treated PDA medium and wood specimen with prepared chemicals. It was not showed anti-mold effectiveness that solvent and emulsifier form preservatives. Therefor, we thought that only four component, dinotefuran, IPBC, propiconazol and clove oil, has anti-mold effectiveness. In the PDA medium test, the preservative which has much IPBC showed high anti-mold effectiveness. By the wood specimen test, it was known that many prepared preservatives which showed good inhibitory effectiveness of mycelial growth in the PDA medium test, have not anti-mold effectiveness. Of all prepared preservatives in this study, only three preservatives containing dinotefuran and IPBC, or adding clove oil appeared the excellent anti-mold effectiveness.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.10
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• pp.1361-1370
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• 2011

Antioxidant and anti-inflammatory activities of eugenol and its derivatives from clove (Eugenia caryophyllata Thunb.) were evaluated using in vitro assay systems by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, cyclooxygenase-2 (COX-2), and 15-lipoxygenase (15-LOX). Among eight different crude medicinal drugs tested, volatile extracts of clove extracted by steam distillation extraction (SDE) showed potent DPPH radical scavenging activity ($IC_{50}$=8.85 ${\mu}g/mL$) as well as strong inhibitory activity against COX-2 (58.15%) and 15-LOX (86.15%) at 10 ${\mu}g/mL$ and 25 ${\mu}g/mL$, respectively. Major volatile components of clove were identified as eugenol, trans-caryophyllene, and acetyleugenol by GC-MS analysis. Out of three eugenol derivatives, eugenol, methyl eugenol, and acetyl eugenol, eugenol showed the strongest DPPH radical scavenging activity and COX-2 inhibitory activity, whereas methyl eugenol exhibited the strongest 15-LOX inhibitory activity. Finally, the contents of the three eugenol derivatives in clove were quantified by analytical HPLC. Contents of eugenol and acetyl eugenol in clove were 6.95% and 1.85% per dry weight, respectively. These results suggest that eugenol and its derivatives in steam distilled extract of clove may be useful as potential antioxidant and anti-inflammatory agents.

• Korean Journal of Veterinary Research
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• v.48 no.1
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• pp.9-16
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• 2008

A method for the quantification of eugenol in the medicinal herb Clove was developed and validated. For preparation of sample solutions clove was dried at $60^{\circ}C$ for 2h and ground by mixer and extracted with 95% ethanol for shaking extraction. The elutes were analyzed by HPLC system included a reversed phase column, a isocratic mobile phase of 60% methanol and PDA detector set at 280 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $0.0125~1{\mu}g/ml$. The limit of detection per sample injection ($20{\mu}l$) was $0.81ng/{\mu}l$ and limit of quantification was $2.47ng/{\mu}l$. The method showed good intra-day precision (%RSD 0.08 ~ 0.27%) and inter-day precision (%RSD 0.32 ~ 1.19%).

• Journal of Fashion Business
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• v.21 no.1
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• pp.166-176
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• 2017

This study is fundamental research on how to be able to scientifically quantify and reproduce a natural dyeing procedure. By dyeing silk fabrics, the establishment of a reproducible dyeing method was sought. Juglans mandshurica has been known as one of the most widely used black vegetable dyes. Repetition conditions and combination dyeing were performed with Juglans mandshurica cortex, gallnut, clove, and Eclipta prostrata L. extracts to express a deep black color. Juglans mandshurica cortex, gallnut, clove, and Eclipta prostrata L. extracts were suitable for black coloration and showed a darker black color when combined with iron mordant. Specifically, Juglans mandshurica cortex and clove can be used for deep black coloring. Color fastness when washed or dry cleaned was found to be strong with a grade of 4-5 and fastness to light was rated at a 3-4. The grade of color change when exposed to rubbing and perspiration was good at a 4-5. In regards to functional property aspects, it showed excellent results with a 99% deodorization rate at 120 minutes of dyeing time, 97.1% UV protection rate, and 85.9% and 62.3% bacterial reduction against Staphylococcus aureus and Streptococcus pneumoniae, respectively. Therefore, it is considered that the extract from the Juglans mandshurica cortex is of great value as an eco-friendly, natural dyestuff.

• Korean journal of food and cookery science
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• v.20 no.5
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• pp.489-497
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• 2004

The purpose of this study was to investigate the characteristics of beef jerky with prepared various spices. The spices added included green tea, rosemary, clove, thyme, and parsley. According to the analysis of general ingredients, the moisture content was higher in the spice-added samples than in the control jerky, and the crude protein content was higher in green tea- and parsley-added samples. According to the mineral analysis, the most prevalent mineral was Na, followed by K, P, Mg, and Ca, with the contents of Mn and Cu being the least prevalent. Ca and Fe contents werehigher in green tea-added jerky than in samples prepared with other spices. High levels of leucine and lysine were seen amongst different essential amino acids. Total amino acids were within the control jerky containing the most abundant essential amino acids, followed by green tea- and parsley-added jerky. Measurements of texture, hardness and springiness in the spice-added samples appeared to be higher than in the control. Bitterness was higher in clove-addedsamples than in samples prepared with other spices. The evaluation of sensory properties showed that color is higher in green tea-, rosemary- and clove-added samples and that taste was best in green tea-added jerky, followed by parsley-added jerky. Overall quality was high in green tea- and parsley-added samples.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.38 no.3 s.116
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• pp.66-71
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• 2006

The objective of this study was to find in what color Hanji(Korean hand-made paper) is dyed when it is dyed with a pigment extracted from clove tree using different kinds of mordant, and how the paper is discolored and variety of strength under the condition of accelerated aging test. The results of this experiment are as follows. The Hanji dyed with aluminum acetate mordant was colored yellowish brown at pH 4.82, discoloration after aging was as much as a slight difference of color was recognized, and the decrease of strength after aging test was small. Thus, the method using aluminum acetate mordant was usable in coloring Hanji. The second most useful Hanji dyeing moth of was using distilled water and ferrous sulfate mordant, which dyed Hanji light brown at pH 6.03. However, when pigment was extracted using distilled water and copper sulfate mordant was used, discoloration was satisfactory but strength decreased too much and pale brown was obtained. Thus, this method was not usable.

• Development and Reproduction
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• v.18 no.1
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• pp.57-64
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• 2014

The flower buds of Syzygium aromaticum (clove) have been used as traditional medicine for the treatment of male sexual disorders in Asian countries. Recently, there are some reports about the effects of the clove on reproductive activities in mammals. Therefore, its effect on testicular function was examined in male golden hamsters whose reproductive activity is inhibited by photoperiod such as winter climate. The male animals were given by daily oral administrations (56 consecutive days) in three doses (4 mg, 20 mg, and 100 mg/kg BW) of the alcoholic extract of the clove. Generally lower dose (4 mg) of the extract continued to keep the reproductive activities of testes. The both middle and high doses (20 mg and 100 mg) of the extract completely inhibited the testicular activity in some animals. Taken together, these results suggest a possible biphasic action of alcoholic extract of Syzygium aromaticum flower bud on testicular function.