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Report on the sediment types, environmental parameters, density and biometry of Manila clam Ruditapes philippinarum in Gyeonggi Bay off the west coast of Korea (경기만 바지락양식장의 저질조성 서식밀도 및 바지락성장의 특성에 관한 보고)

  • Park, Kwang-Jae;Choi, Yong-Suk;Heo, Seung;Kang, Hee-Woong;Han, Hyun-Seob;O, Hae-Chong
    • The Korean Journal of Malacology
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    • v.26 no.4
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    • pp.267-273
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    • 2010
  • From January to December 2007, we have investigated sediment types, environmental factors and density of clam Ruditapes philippinarum inhabited at Oeri, Naeri, Jonghyeon and Bangmiri tidal flats in Gyeonggi Bay off the west coast of Korea. Sediment types of Oeri and Jonghyeon were characterized with well sorted muddy sand. In contrast, Bangmiri tidal flat was mainly composed of poorly sorted gravelly muddy silt and Naeri was characterized with very poorly sorted muddy-sandy gravel. During the course of study, the surface water temperature, salinity, dissolved oxygen and pH ranged 3.2 to $27.0^{\circ}C$, 21.7 to 33.3 ‰, 5.6 to 12.7 mg/l and 7.36 to 8.82, respectively. The suspended solid (SS) ranged from 3.2 to 1,266.0 mg/l and chlorophyll-a level in the water column ranged $0.3-36.1{\mu}g/l$, respectively. The density of clam in the study areas was mainly determined by the harvesting activities rather than the types of substrate. Relatively higher density of clam was observed in Oeri and Bangmiri where the clam seeds were sowed by the villagers. Condition factor of clams were found to be higher in Oeri where the substrate sorting was better with higher level of chlorophyll a. It was believed that sediment types and the level of available food the two governing factors controlling the density and growth of clam in Gyeonggi Bay.

Effect of Water Temperature, Salt and $MgCl_2$, Concentration on Sand Ejection Characteristics of Short Neck Clam, Luditapes philippinarum (수온, 염분 및 염화마그네슘의 농도가 바지락의 토사특성에 미치는 영향)

  • HONG Sang-Pill;KIM Dong-Su;KIM Young-Myung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.1
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    • pp.114-118
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    • 1997
  • Effect of water temperature, salt and $MgCl_2$ concentration on sand ejection characteristics of short neck clam, Luditapes philippinarum was investigated. Unlike other shell fish such as red shell, arkshell and surf clam, treatment of short neck clam with sea water was evaluated not effective as sand ejection conditions. Sand ejection activity of short neck clam was shown effective at $2.5\%$ NaCl (pH 8.0) at $25^{\circ}C$. This activity was enhanced about 1.57 times when 50 mM $MgCl_2$ were added to the above mentioned conditions. But the extent of sand ejection activity was shown higher in the order of sea water $(3.2\%\;salt)+20mM\;MgCl_2$, sea water $(3.2\%\;salt),\;2.5\%\;NaCl+50mM\;MgCl_2$, treatments. Therefore, it was suggested that habitat conditions and Mg ions could be responsible for biological activity and concominant sand ejection of short neck clam.

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Study of the Food Characteristics on Pacific Oyster Crassostrea gigas and Manila Clam Ruditapes phillippinarum in the Intertidal Zone of Taeahn, Korea (태안 조간대에 서식하는 참굴과 바지락의 먹이특성에 관한 연구)

  • Baek, Seung-Ho;Lee, Ju-Yun;Lee, Hea-Ok;Han, Myung-Soo
    • Korean Journal of Environmental Biology
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    • v.26 no.3
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    • pp.145-158
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    • 2008
  • To assess the source of oyster (Crassostrea gigas) and clam (Ruditapes phillippinarum), phytoplankton community structures in the water column and sediment, including the composition of phytoplankton in oyster and clam digestive organs were investigated bimonthly from December 2006 to June 2007 in the Taeahn coastal waters. During the sampling period, water temperature and salinity varied from 7 to 23$^{\circ}C$ and 34 to 35 PSU, respectively. Total phytoplankton abundances at St. O in the water column were higher than those at St. J, whereas total phytoplankton abundances at St. O in the sediment were lower. In addition, total phytoplankton abundances in the water column and sediment were observed to be relatively higher in February and April 2007. Among the diatoms, Paralia sulcata was always dominant, accounting for 41$\sim$87% of total phytoplankton, except St. J for February 2007 during the sampling period. The following phytoplankton compositions observed in the digestive organs of oyster and clam appeared: diatoms such as genus Paralia, Navicula, Melosira and Coscinodiscus, Silicoflagellate Dictyocha, dinoflagellates Prorocentrum and Dinophysis. Phytoplankton compositions observed in the digestive organs of oyster and clam corresponded relatively well with the species composition appeared in the water column and sediments of each season. A significant relationship was found between individual weight of oyster or clam and their digestive organs weight, while there was not a close correlation with total phytoplankton amount. We suggest that P. sulcata always dominated as one of important the food source of oyster and clam in the marine ranching ground of Taeahn coastal waters.

Sedimentological and Hydromechanical Characteristics of Bed Deposits for the Cultivation of Manila clam, Ruditapes philippinarum in Gomso Tidal Flat (곰소만 조간대 바지락 양식장 저질의 퇴적학적 및 수리역학적 특성)

  • CHO Tae-Chin;LEE Sang-Bae;KIM Suck-Yun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.3
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    • pp.245-253
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    • 2001
  • To investigate the effects of hydromechanical and textural characteristics of sediment deposits on the cultivation of Manila clam, Ruditapes philippinarum surface and sub-surface core sediments were collected seasonally in Gomso tidal flat. Grain size distribution were analyzed to investigate the annual variation of sediment texture. In winter unimodal distribution of grain size with the peak at $5\phi$ is dominant However, during the summer sediment texture become a little bit coarser and grain size distribution shows the peaks at $4\~5 \phi$. Optimum sediment texture for the cultivation of manila clam, R. philippinarum was found to be sandy silt in which mean Brain size was between 4 and $5 \phi$ with the sand content less than $50\%$ and clay content of $5\~10\%$. Mechanical and hydrological characteristics of sediment deposits were also studied in the laboratory and the results were applied to the numerical simulation for the behavior of surface sediment subjected to the cyclic loading from sea-water level change. Results of numerical simulation illustrate that the permeability of sediment had to be maintained in the range of $10^{-11}\sim10^{-12}m^2$ to ensure the proper sedimentological environment for the cultivation of manila clam, R. philippinarum. The deposits of virtually impermeable mud layer, with the threshold thickness of 4 cm, would be very hazardous to clam habitat.

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Study on the Habitat Environment of Sulf Clam, Tresus Keenae (왕우럭조개의 서식환경에 관한 연구)

  • Kim, C.W.;Jeong, D.S.;Choi, S.J.;Kang, H.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.23 no.2
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    • pp.15-24
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    • 2021
  • For the aquaculture industrialization of surf clam (Tresus keenae), it is important to basic data on the marine environment of the habitat of surf clam (T. keenae). In this study, we investigated the marine environment of habitat of surf clam (T. keenae) and sought to basic data for the preparation of surf clam (T. keenae) for artificial seed production. The water temperature of the habitat of surf clam (T. keenae) was the lowest in winter and appeared high in summer. The salt concentration showed it range from 31.2 to 33.9 psu. The pH showed it range from 7.69 to 8.70, with high pH in winter and low pH in summer. The dissolved oxygen(DO) was showed it range from 6.20 to 10.24 mg / L and the autumn was relatively higher than the spring and winter. The species composition of phytoplankton was about 30 to 40 species, and most of them were diatoms. The abundance of seasonal phytoplankton showed it range from 23.5 to 61.3 cells / ml, showing seasonal differences. The expression of dominant species also showed a difference depending on the season. As for the particle size composition of the sediment, sandy silt was the most distributed. Flow velocities appeared at 50-80 cm / s in the southeast direction at ebb tide and at 60-100 cm / s in the northwest direction at flood tide. The results of this study can be used as basic data for providing knowledge about the habitat and marine environment of surf clam (T. keenae) and for studying shellfish that inhabit the sedimentary layer.

Peptide Inhibitor for Angiotensin-Converting Enzyme from Thermolysin Hydrolysate of Manila Clam Proteins

  • Lee Tae-Gee;Yeum Dong-Min;Kim Young-Sook;Yeo Saeng-Gyu;Lee Yong-Woo;Kim Jin-Soo;Kim In-Soo;Kim Seon-Bong
    • Fisheries and Aquatic Sciences
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    • v.8 no.2
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    • pp.109-112
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    • 2005
  • A peptide that inhibits angiotensin-converting enzyme (ACE) was isolated from a hydrolysate of Manila clam (Ruditapes philippinarum) proteins prepared with thermolysin. Amino acid sequence of the peptide was determined to be Leu-Leu-Pro. Chemically synthesized Leu-Leu-Pro had an $IC_{50}\;value\;of\;158\;\mu{M}$. Peptides related to the Manila clam-derived peptide were synthesized to study the structure-activity relationships. The tetrapeptide, Leu-Leu-Pro-Pro, had a very weak effect on the enzyme. However, Leu-Leu-Pro-Asn showed no inhibitory activity.

Effects of electroslag remelting process and Y on the inclusions and mechanical properties of the CLAM steel

  • Qiu, Guoxing;Zhan, Dongping;Li, Changsheng;Yang, Yongkun;Jiang, Zhouhua;Zhang, Huishu
    • Nuclear Engineering and Technology
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    • v.52 no.4
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    • pp.811-818
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    • 2020
  • Y-containing CLAM steels were melted via vacuum induction melting and electroslag remelting. In this study, the evolution, microstructure, and mechanical properties of the alloy inclusions (ESR-1 (0 wt.% Y), ESR-2 (0.016 wt.% Y) and ESR-3 (0.042 wt.% Y)) were investigated. Further, the number of inclusions in ESRed steel was observed to obviously decrease, and the distributions were more uniform. The fine Y-Al-O inclusions (1-2 ㎛) were the main inclusions in ESR-2. The addition of Y affected the prior austenite grain size (PAGZ), increasing the tensile strength at test temperature. Low ductile-brittle transition temperature (DBTT) was obtained because of the fine PAGZ and dispersive inclusions. For the ESRed CLAM steel with 0.016 wt.% Y, the yield strengths were 621 MPa at 20 ℃ and 354 MPa at 600 ℃ in air. Further, the uniform elongation and elongation of the ESR-2 alloy were 5.5% and 20.1% at 20 ℃, respectively. Meanwhile, the DBTT tested using full-size Charpy impact specimen (55 cm × 10 cm × 10 cm) was reduced to -83 ℃.

Prevalence, infection intensity and histopathology ofPerkinsus in Venus clam, Protothaca jedoensis from Yo-su, Korea

  • Ngo, Thao T.T.;Park, Kyung-Il;Lim, Na-Rae;Choi, Sang-Duk;Choi, Kwang-Sik
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2004.05a
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    • pp.512-512
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    • 2004
  • Venus clam, Protothaca jedoensis was collected in lune 2003 from Yosu, Korea. The prevalence and infection intensity of Perkinsus was determined by Ray's Fluid thioglycollate medium technique and Choi's 2 M NaOH digestive assay. The prevalence of infection was 80% in Yosu; 54.2% in Taejon and 15.5% in Sachon. Consequently, infection intensity was highest in clams from Yosu (6250${\pm}$8188 Perkinsus cells/g of tissue) whereas lower levels were found in Taejon (5072${\pm}$10862 Perkinsus cells/g of tissue) and in Sachon (2452${\pm}$15300 Perhnsus cells/g of tissue). Histological observation showed that Perkinsus presented in mantle, gills, digestive tubes and gonad of clams. The hemocytic infiltrations and tissue damages were found in heavy Perkinsus-infected clams. Results after immunofluorescence staining with antibody developed from Perkinsus atlanticus suggested that there is closed relationship between Perkinsus found in Venus clam and Manila clam.

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Cadmium Accumulation and Elimination in the Tissues of the Manila Clam, Ruditapes philippinarum, after Sub-chronic Cadmium Exposure

  • Jang, Seok-Woo;Kim, Seong-Gil;Choi, Ok-In;Kim, Seong-Soo;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.324-330
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    • 2009
  • Cadmium (Cd) accumulation and elimination were assessed in the tissues of the clam R. philippinarum at four experimental concentrations (control, 10, 20, 100, and $200\;{\mu}g/L$) over an exposure period of 2 weeks and an elimination period of 1 week. Cd accumulated in the digestive gland, gill, and residual clam tissues, and accumulation increased with time of exposure and concentration (100 and $200\;{\mu}g/L$). After 2 weeks of Cd exposure, the order of Cd accumulation in tissues was gill > digestive gland > residual tissues. An inverse relationship was observed between concentration factor (CF) and exposure level, but the CF showed an increase with exposure time. During the depuration period, Cd concentrations in the digestive gland, gill, and residual tissues decreased immediately on the cessation of exposure, except in individuals at the $200\;{\mu}g/L$ concentration. The Cd elimination rate from tissues decreased in the order of digestive gland > gill > residual tissues during the depuration period.

Use of Squilla (Orato squilla nepa), Squid (Sepia pharonis) and Clam (Katelysia opima) Meal Alone or in Combination as a Substitute for Fish Meal in the Postlarval Diet of Macrobrachium rosenbergii

  • Naik, S.D.;Sahu, N.P.;Jain, K.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.9
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    • pp.1272-1275
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    • 2001
  • Two hundred and forty post-larvae (PL) of fresh water prawn, Macrobrachium rosenbergii were distributed in eight treatment groups with three replicates each. Eight experimental diets were prepared by using squilla (Orato squilla nepa), squid (Sepia pharonis) and clam (katelysia opima) alone or in different combination as source of animal protein and compared to a control diet containing fish meal. Total crude protein content for all dietary treatments was around 32%. Total protein content of fish meal was replaced by an equal amount of protein from different animal protein sources on isonitrogenous basis. Diets were fed at 5% of the body weight of post-larvae twice daily. The experiment was conducted for a period of 60 days. It was found that all the above three protein sources could be used by completely replacing fishmeal except clam meal at higher level of inclusion (26%). A combination of squid and squilla meal at 14% each in the diet increased the growth performance of PL significantly (p<0.05) in terms of Specific growth rate (SGR) % (5.17), FCR (2.12) and PER (1.51). Squilla meal can be used to the maximum level of 38% without any growth depression.