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http://dx.doi.org/10.5657/fas.2005.8.2.109

Peptide Inhibitor for Angiotensin-Converting Enzyme from Thermolysin Hydrolysate of Manila Clam Proteins  

Lee Tae-Gee (Department of Hotel Culinary & Baking, Namdo Provincial College of Jeonnam)
Yeum Dong-Min (Department of Food Processing & Baking, Yangsan College)
Kim Young-Sook (Department of Food Processing & Baking, Yangsan College)
Yeo Saeng-Gyu (Department of Hotel Culinary Arts, Busan College of Information Technology)
Lee Yong-Woo (Division of Food Science, Dongeui Institute of Technology)
Kim Jin-Soo (Division of Marine Bioscience Institute of Marine Industry, Gyeongsang National University)
Kim In-Soo (Division of Marine Bioscience Institute of Marine Industry, Gyeongsang National University)
Kim Seon-Bong (Faculty of Food and Biotechnology, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.8, no.2, 2005 , pp. 109-112 More about this Journal
Abstract
A peptide that inhibits angiotensin-converting enzyme (ACE) was isolated from a hydrolysate of Manila clam (Ruditapes philippinarum) proteins prepared with thermolysin. Amino acid sequence of the peptide was determined to be Leu-Leu-Pro. Chemically synthesized Leu-Leu-Pro had an $IC_{50}\;value\;of\;158\;\mu{M}$. Peptides related to the Manila clam-derived peptide were synthesized to study the structure-activity relationships. The tetrapeptide, Leu-Leu-Pro-Pro, had a very weak effect on the enzyme. However, Leu-Leu-Pro-Asn showed no inhibitory activity.
Keywords
Angiotensin-converting enzyme (ACE); Manila clam; thermolysin hydrolysate; ACE- inhibitory peptide;
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