• Journal of the Korean Applied Science and Technology
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• v.30 no.3
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• pp.394-399
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• 2013

This study was performed to investigate the antidiabetic study effect in ethanol extract of Ailanthicortex(A.C) radici in Streptozotocin(STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45mg/kg dissolved in citrate buffer. The ethanol extract of A.C radici was orally administrated once a day for 7 days at a dose of 500mg/kg. The content of serum glucose, triglyceride(T.G), total cholesterol were significantly decreased in treated group compared to the those of STZ-control group. The content of hepatic glycogen and activity of glucokinase(GK) were significantly increased in treated group compared to the those of STZ-control group. but activity of glucose-6-phosphate dehydrogenase(G-6-PDH) was significantly increased. These results indicated that ethanol extract of A.C would have antidiabetic effect in STZ-induced diabetic rats.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.130.3-131
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• 2003

The biological effects of NADPH-dependent isocitrate dehydrogenase (IDPc) inhibitor. DA-11004, was examined in obese zucker rats or streptozotocin-induced diabetic SD rats. Diabetes was induced by injection of streptozotocin (50mg/kg) dissolved in citrate buffer (pH 4.8) into the tail vein and induction of diabetes was confirmed by the measurement of the tail blood glucose level at 48h. DA-11004 (30mg/kg, po) was injected for successive 7days and significantly reduced the plasma glucose in streptozotocin-induced diabetic rats (P<0.05). (omitted)

• Bulletin of the Korean Chemical Society
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• v.32 no.9
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• pp.3411-3420
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• 2011

Kinetic and structural studies have been carried out on the effects of meso-tetrakis(4-sulfonatophenyl)-porphyrin ($H_2TPPS_4$) as an anionic and meso-tetrakis(3-N-methyl-pyridyl)porphyrin ($H_2TMPYP$) as a cationic porphyrin with adenosine deaminase (ADA) in 25 mM citrate/phosphate buffer, pH = 4-8, at $37^{\circ}C$ using UVvis spectrophotometry, circular dichroism (CD), fluorescence spectrophotometry as well as molecular dynamics (MD) and molecular docking. Kinetic results showed that the two porphyrins are non-competitive inhibitors. Increasing pH, increases $K_I$ and cationic porphyrin has a higher $K_I$ and lower binding constant ($K_b$) at all pH ranges. Analyzing the secondary structure revealed that both ligands decrease the secondary structure and that the anionic porphyrin is more effective.

• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.96-102
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• 1998

Primary hepatocytes of small animals such as rat and rabbit were often used for the study of extracorporeal liver support systems. Freshly isolated rat hepatocytes form spheroids within tow days when cultivated as suspension in spinner vessels. These spheroids showed enhanced liver specific functions and more differentiated morphology compared to hepatocytes cultured as monolayers However, shear stress caused by continuous agitation deteriorated spheroids gradually. In this work we immobilized spheroids to prolong liver specific activities. First, hepatocyte spheroids were suspended in collagen solution containing calcium chloride and then dropped into alginate solution. A thin layer of calcium alginate was formed around the droplet and then was removed after the inner collagen was gelled by treatment of sodium citrate buffer. Spheroids embedded in collagen-gel bead maintained liver specific functions such as albumin secretion rate longer than hepatocyte spheroids exposed to shear stress. Therefore, we suggest that this immobilization technique may offer an effective long-term hepatocyte cultivation and facilitase the development of a bioartificial liver support device.

• KSBB Journal
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• v.30 no.2
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• pp.53-57
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• 2015

In this study, the production of total reducing sugar from macro green-algae Enteromorpha intestinalis by enzymatic hydrolysis was investigated. As a result of enzymatic hydrolysis using 13 kind commercial enzymes, the highest yield of 8.75% was obtained from Viscozyme L, which is multi-enzyme complex such as cellulase, arabanase, beta-glucanase, hemicellulase and xylanase. As a control, only 0.33% and 0.27% yield were obtained from 1% sulfuric acid and 0.05 M citrate buffer (pH 4.8), respectively. In the case of enzyme mixture, the mixture of $Viscozyme^{(R)}$ L and $Cellic^{(R)}$ CTec2 (1:1) was presented the highest yield of 10.67%. Finally, the 14.99% yield was obtained at 36 hr under the condition of 10% biomass and 30% enzyme mixture.

• Korean Journal of Pharmacognosy
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• v.40 no.3
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• pp.213-217
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• 2009

This study was done to investigate the antioxidative effect of polygoni radix in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45 mg/kg dissolved in citrate buffer. The ethanol extract of Polygoni radix was orally administrated once a day for 7 days. The contents of Total cholesterol, triglyceride (TG) were significantly decreased in Polygoni radix treated STZ-sample group compared with to the those of STZ-control group. The hepatic cytosolic activities of glutathione-s-transferase (GST), superoxide dismutase (SOD) were significantly increased, Also the content of glutathione (GSH) was incresaed in Polygoni radix treated STZ-sample group compared with to the those of STZ-control group. but the content of malondialdehyde (MDA) and the hepatic cytosolic activity of Catalase (CAT) were decreaed but not statistical significant. These results indicated that ethanol extract of polygoni radix was effective for the antidxidative in the STZ-induced diabetic rats.

• Journal of Embryo Transfer
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• v.10 no.2
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• pp.145-151
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• 1995

초기배의 성판정은 대상가축의 성을 선발하는 수단으로써 가축의 육종 및 번식에 있어 가치가 매우 높다. 체세포, 체외수정 또는 처녀발생 초기배의 성을 결정하기 위해 capillary polymerase chain reaction (PCR)을 이용하였으며 성판정에 이용되는 상실배 또는 배반포는 체외수정과 그 후의 난관상피세포와의 공배양에 의해 생산되었다. 초기배의 genomic DNA는 0.2$\mu$g/$\mu$L proteinase K를 함유하고 있는 PCR lysis buffer에 하나의 초기배를 부유하게 50˚C 에서 1시간동안 배양한 후 95˚C에서 10분간 효소를 비활성화시킴으로써 준비되었다. 웅성 초기배에서는 두개의 증폭산물(소특이)만이 생산되었다. 이 기법에 의한 성판정 결과 초기배의 성비는 예상되는 1:1의 성비와 유의적인 차이가 없었다. 잔여 난구세포 또는 투명대에 결합된 정자 등으로 인한 잘못된 성판정이 종종 발생하였는데, 이는 citrate 처리후 투명대를 완전히 제거함으로써 false positive 또는 negative 결과를 극복할 수 있었다. 이상과 같은 결과는 체외생산 소 초기배의 신속하고(2시간 증폭) 정확한 성판정의 가능성과 초기배 이외의 세포로부터의 오염이 확립된 수세방법에 의해 효과적으로 배제될 수 있음을 제시하였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.5
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• pp.908-913
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• 1997

The purpose of this study was to investigate phospholipase $A_{2}$ activity and lipid peroxidation I streptozotocin induced diabetic rats. Sprague-Dawley male rats weighting-Dawley male rats weighting 300$\pm$10gm were randomly assigned to normal and STZ-induced diabetic group. Diabetes was induced by intravenous injection of 55mg/kg of STZ in sodium citrate buffer(pH 4.3). Animals were sacrificed at the 6th day of diabetic states. Body weight gains were lower in DM group. Phosphatidylcholine hydrolysis in liver was not significantly different between two groups, whereas phosphatidylethanolamine hydrolysis in liver was increased by 69% in DM group comparing with that of normal group. Liver microsomal phospholipase $A_{2}$ activity and level of TBARS was increased by 91%, 109% in DM group compared with that of normal group, respectively. The present results indicate that phospholipase $A_{2}$ activity is specific to PE hydrolysis, leading to lipid peroxidation process in STZ induced diabetic rats.

• Journal of the Korean Applied Science and Technology
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• v.25 no.3
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• pp.347-354
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• 2008

This study was done to investigate the antidiabetic effect of polygoni radix in Streptozotocin(STZ)-induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45mg/kg dissolved in citrate buffer. The ethanol extract of polygoni radix was orally administrated once a day for 7 days. The content of serum glucose, triglyceride(TG), total cholesterol were significantly decreased in polygoni radix treated STZ-sample group compared to the those of STZ-control group. The content of hepatic glycogen and activities of glucose-6-phosphate dehydrogenase(Glucose-6-PDH), glucokinase were significantly increased, but activity of glucose-6-phoshatase was decreased in polygoni radix treated STZ-sample group compared to the those of STZ-control group. These results indicated that ethanol extract of polygoni radix would have antidiabetic effect in STZ-induced diabetic rats.

• Microbiology and Biotechnology Letters
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• v.21 no.3
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• pp.200-206
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• 1993

The effect of pH (7, 5 and 4) and nisin (100 and 200IU/ml) on heat resistance of Listeria monocytogenes Scott A were determined using citrate-phosphate buffer system. Heat resistance of vegetative and starved cell was decreased as pH value was lower at 65 and 72C. Starved L. monocytogenes was more resistant than vegetative cell at both temperature. Heat resistance of vegetative and starved cell was decreased significantly with treatment of nisin. The effect of nisin was increased significantly at low pH(5, 4). Adherent microcolony was more resistant to heat and nisin than planktonic cell. Contamination of L. monocytogenes may be prevent by using nisin in food and food processing environments.