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http://dx.doi.org/10.5012/bkcs.2011.32.9.3411

Effect of Cationic and Anionic Porphyrins on the Structure and Activity of Adenosine Deaminase  

Ajloo, Davood (School of Chemistry, Damghan University)
Hajipour, Samaneh (School of Chemistry, Damghan University)
Saboury, Ali Akbar (Institute of Biochemistry and Biophysics, The University of Tehran)
Zakavi, Saeed (Department of Chemistry, Institute for Advanced Studies in Basic Sciences)
Publication Information
Bulletin of the Korean Chemical Society / v.32, no.9, 2011 , pp. 3411-3420 More about this Journal
Abstract
Kinetic and structural studies have been carried out on the effects of meso-tetrakis(4-sulfonatophenyl)-porphyrin ($H_2TPPS_4$) as an anionic and meso-tetrakis(3-N-methyl-pyridyl)porphyrin ($H_2TMPYP$) as a cationic porphyrin with adenosine deaminase (ADA) in 25 mM citrate/phosphate buffer, pH = 4-8, at $37^{\circ}C$ using UVvis spectrophotometry, circular dichroism (CD), fluorescence spectrophotometry as well as molecular dynamics (MD) and molecular docking. Kinetic results showed that the two porphyrins are non-competitive inhibitors. Increasing pH, increases $K_I$ and cationic porphyrin has a higher $K_I$ and lower binding constant ($K_b$) at all pH ranges. Analyzing the secondary structure revealed that both ligands decrease the secondary structure and that the anionic porphyrin is more effective.
Keywords
Porphyrin; Binding constant; Fluorescence; Circular dichroism; Inhibition constant;
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