• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
• /
• pp.274.1-274.1
• /
• 2013

We report the circumferential alignment of human aortic smooth muscle cells (HASMCs) in an orthogonally micropatterned circular microfluidic channel to form an in vivo-like smooth muscle cell layer. To realize a biomimetic smooth muscle cell layer which is aligned perpendicular to the axis of blood vessel, we first fabricated a half-circular polydimethylsiloxane (PDMS) microchannel by soft lithography using a convex PDMS mold. The orthogonally micro wrinkle patterns were generated inside the half-circular microchannel by stretching-releasing operation under UV irradiation. Upon UV treatment with uniaxial 40 % stretch of a PDMS substrate and releasing process, the microwrinkle patterns perpendicular to the axial direction of the circular microchannel were generated, which could guide the circumferential alignment of HASMCs successfully during cultivation. The analysis of orientation angle, shape index, and contractile protein marker expression indicates that the cultured HASMCs revealed the in vivo-like cell phenotype. Finally, we produced circular microchannels by bonding two half-circular microchannels, and cultured the HASMCs circumferentially with high alignment and viability for 5 days. These results are the first demonstration for constructing an in vivo-like 3D smooth muscle cell layer in the circular microfluidic channel which can provide novel bioassay platforms for in-depth study of HASMC biology and vascular function.

• Structural Engineering and Mechanics
• /
• 제77권1호
• /
• pp.137-149
• /
• 2021

In engineering design, the axial equivalent elastic modulus of laminated FRP pipe was mostly calculated by the average elastic modulus method or the classical laminated plate theory method, which are based on relatively simplified assumptions, and may be not accurate enough sometimes. A new analytical calculation method for the axial equivalent elastic modulus of laminated FRP pipe was established based on three-dimensional stress state. By comparing the results calculated by this method with those by the above two traditional analytical methods and the finite element method, it is found that this method for the axial equivalent elastic modulus fits well not only for thin-walled pipes with orthotropic layers, but also for thick-walled pipes with arbitrary layers. Besides, the influence of the layer stacking on the axial equivalent elastic modulus was studied with this method. It is found that a proper content of circumferential layer is beneficial for improving the axial equivalent elastic modulus of the laminated FRP pipe with oblique layers, and then can reduce its material quantity under the premise that its axial stiffness remains unchanged. Finally, the meso-mechanical mechanism of this effect was analyzed. The improving effect of circumferential layer on the axial equivalent elastic modulus of the laminated FRP pipe with oblique layers is mainly because that, the circumferential fibers can restrain the rigid body rotations of the oblique fibers, which tend to cause the significant deformations of the pipe wall units and the relatively low axial equivalent elastic modulus of the pipe.

• 동력기계공학회지
• /
• 제18권5호
• /
• pp.100-105
• /
• 2014

It has been previously reported that in principle sludge and blockages can be detected and even characterized by using guided ultrasonic torsional waves, based on an idealized model in which the sludge layer was simplified in terms of geometry and material properties. The work revealed that the presence of a layer inside a pipe scatters the guided wave propagating in the pipe and both the reflection and transmission of the guided wave can be used to effectively detect and characterize the layer. This paper proceeds the work by taking into account more realistic sludge characteristics, including irregular circumferential profiles of the sludge layer and imperfect bonding state between the sludge and the pipe. The influence of these issues is investigated to identify the critical factors that influence the detection and characterization capability of the two measurements.

• Advances in concrete construction
• /
• 제13권4호
• /
• pp.327-337
• /
• 2022

This paper is concerned with the vibration analysis of middle layer cylindrical shell made of functionally graded material. The outer layers and inner layer are composed of functionally graded and isotropic material respectively. The Rayleigh Ritz method is applied to solve the presented shell dynamics equations. Two configurations are constructed with layers distributions. Fundamental natural frequencies of the three layered cylindrical shell is plotted against the circumferential wave number with different power law exponents. The frequency decreases with the increase of power law exponent. The fundamental natural frequencies first decreases and fall down to its minimum value, after frequencies increases with circumferential wave number. This is due to change in the magnitude of extensional and bending energies of the cylindrical shells. The computer software MATLAB has been employed for the computation of presented frequencies and tested the results obtained in order to assess the accuracy and validity of the cylindrical shell model for predicting the vibration frequencies of cylindrical shell.

• 비파괴검사학회지
• /
• 제19권3호
• /
• pp.189-199
• /
• 1999

산화막이 피복된 원전 연료봉의 원통형 쉘에 대한 공명산란 해석을 수행하고 원주파 전파 특성을 연구하였다. 피복 쉘에 대한 산란 음압의 정규 모우드 해를 구하였고 최근에 새롭게 제안된 고유 배경음압 계수를 이용하여 피복 쉘의 순수 공명 신호를 분리하였다. 12%의 상대 두께를 갖는 원통형 피복 쉘에 대하여 산화막 두께 증가에 따른 공명 원주파의 전파 특성을 해석하였다. 산화막의 존재와 그 두께가 증가할 때 정규 모우드의 차수에 따라 원주파의 전파 특성이 크게 변화한다. 제 1차 반대칭 ($A_1$) 원주파에서 특정 부분파의 위상속도는 산화막이 존재하고 그 두께가 증가함에도 불구하고 위상속도가 일정 한 특성을 보인다. 공명신호를 분리하고 공명 모우드를 확인하는 실험을 수행하여 $A_1$ 원주파의 전파 특성을 확인하였다. $A_1$ 원주파의 위상속도 일정 특성을 이용함으로써 산화막의 두께를 상대적으로 측정 할 수 있는 새로운 비파괴 평가방법을 제안하였다.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2000년도 춘계학술대회논문집
• /
• pp.606-611
• /
• 2000

It has been noted that the low frequency absorption coefficient of a porous sample placed in a standing wave tube is affected by the nature of the sample's edge constraint. The edge constraint has the effect of stiffening the solid phase of the sample, which itself can be strongly coupled to the material's fluid phase, and hence the incident sound field, by viscous means at low frequencies. In recent work it has also been shown that such a circumferential constraint causes the low frequency transmission loss of a layer of fibrous material to approach a finite low frequency limit that is proportional to the flow resistance of the layer and which is substantially higher than that of an unconstrained sample of the same material. However, it was also found that the benefit of the circumferential edge constraint was reduced in a transitional frequency range by a shearing resonance of the sample. Here it will be shown that the effect of that resonance can be mitigated or eliminated by adding additional axial and radial constraints running through the sample. It will also be shown that the constraint effect can be modeled closely by using a finite element procedure based on the Biot poroelastic theory. Implications for low frequency barrier design are also discussed.

• 대한조선학회지
• /
• 제23권1호
• /
• pp.13-22
• /
• 1986

Axisymmetric turbulent thick boundary layers on a rotating body of revolution are calculated numerically in the paper. Richardson number is introduced to the mixing length to take account of swirl effects on Reynolds stresses. Interactions of the boundary layer and the external potential flow are included by adding the displacement thickness of boundary layers on the original body. Pressure distributions on the body surface are estimated by integrating normal momentum equation across the boundary layer. A model is designed and tested in the wind tunnel. Mean velocities are measured. Through the present study, swirl effects on the thick axisymmetric boundary layer development are considerable in comparison with those of non-totating cases. Rotational motion generally increase boundary layer thickness, axial skin friction coefficients, and form drags. Circumferential flow can be reversed to induce negative skin friction when the section area is reduced.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2008년 영문 학술대회
• /
• pp.66-71
• /
• 2008

Two cases with circumferential grooves were designed for a transonic compressor, and 3-D numerical simulations were conducted for stall mechanism at three representative speeds. A conclusion can be drawn from the comparison between compressors with or without casing treatment that: with the rising of rotation speed, stall margin increases dramatically under the help of casing treatments, and the case with middle grooves has reasonable compromise between stall margin increment and efficiency cutting. At lower speed, the increment reduces, and grooves at the back of blade tip have more influence on stall margin. Further investigation shows there is a transition in mechanism of compressor stall with the decline of rotational speed: at high rotation speed, the expansion of stall margin mainly results from the suppression of tip leakage vortex by casing treatments, yet it benefits more from the depression of boundary layer separation from suction surface of blade tip.

• 대한기계학회논문집
• /
• 제19권4호
• /
• pp.981-989
• /
• 1995

A new weight function approach to determine SIF(stress intensity factor) using single-layer potential has been presented. The crack surface displacement field was represented by one boundary integral term whose kernel was modified from Kelvin's fundamental solution. The proposed method enables the calculation of SIF using only one SIF solution without any modification for the crack geometries symmetric in two-dimensional plane such as a center crack in a plate with or without an internal hole, double edge cracks, circumferential crack or radial cracks in a pipe. The application procedure to those crack problems is very simple and straightforward with only one SIF solution. The necessary information in the analysis is two reference SIFs. The analysis results using present closed-form solution were in good agreement with those of the literature.

• 대한수의학회지
• /
• 제39권3호
• /
• pp.407-416
• /
• 1999

This study was carried out to investigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into $40{\mu}m$-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae : an olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and a layer of white matter. The accessory olfactory bulb had four layers : an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region.