• 제목/요약/키워드: cilia

검색결과 154건 처리시간 0.023초

세포의 섬모 운동 변화 분석을 위한 CBF 분포도 구성 및 국소적 분포 분석에 관한 연구 (Distribution Mapping and Local Analysis of Ciliary Beat Frequencies)

  • 이원진;박광석;민양기;성명훈;이강수
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1997년도 추계학술대회
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    • pp.154-160
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    • 1997
  • By their rapid and periodic actions, the cilia of the human respiratory tract play an important role in clearing inhaled noxious particles. Based on the automated image-processing technique, we studied ciliary beat frequency (CBF) objectively and quantitatively. Microscopic ciliary images were transformed into digitized gray ones through an image-grabber, and from these we extracted signals or CBF. By means of a FFT, maximum peak frequencies were detected as CBFs in each partitioned block or the entire digitized field. With these CBFs, we composed distribution maps visualiy showing the spatial distribution of CBFs. Through distribution maps of CBF, the whole aspects of CBF changes or cells and the difference of CBF of neighboring cells can be easily measured and detected. Histogram statistics calculated from the user-defined polygonal window can show the local dominant frequency presumed to be the CBF of a cell or a crust the region includes.

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호르몬을 투여한 Rat 난관상피세포(卵管上皮細胞)의 전자현미경적 관찰 (Electron Microscopic Observations of Oviductal Epithelium of the Rats Treated with Hormone)

  • 이재현
    • 대한수의학회지
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    • 제22권2호
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    • pp.99-109
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    • 1982
  • Morphological changes of the oviductal epithelium of the rat treated with hormones ($17{\beta}$-estradiol ${\mu}g$/day and progesterone 2.5mg/day) for ten days were observed transmission and scanning electron microscopically. The results obtained were as follows: 1. The cilia formation of ciliary cell(CC) was more accelerated by the treatment of estradiol than progesterone, but the balance of estrogen and progesterone was required for the maintenance of CC. The effect of hormone was different between the segments for the maintenance of CC. 2. The short secretory cell(SSC) was severely inhibited in the formation of secretory granules with single hormonal treatment but the activity of secretion was more inhibited by progesterone than by estradiol. 3. The long secretory cell(LSC) had not a great difference between estradiol and progesterone treatments as compared with the normal sexual cycle, but the formation of secretory granules was somewhat accelerated by progesterone treatment. 4. The formation of secretory granules of junctional cell (JC) was severely accelerated by estradiol treatment as compared with the normal sexual cycle. The formation of secretory granules during progesterone treatment, on the other hand, was inhibited completely, but the numbers of pinocytotic vesicles appeared at the cytoplasmic apical portion. 5. Three types of secretory cells, SSC, LSC and JC, on the rat oviductal epithelium could be suggested to have different cell tapes respectively from the morphological changes by hormone treatment.

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Selaginella subvaginata (Selaginellaceae), a new spikemoss from China

  • Zhang, Xian-Chun;Shalimov, Aleksandr Petrovich;Kang, Jong-Soo;Zhang, Meng-Hua
    • Journal of Species Research
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    • 제9권3호
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    • pp.221-232
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    • 2020
  • Selaginella vaginata is a common montane species with broad distribution in China and the Himalaya region, and several species that are morphologically similar to S. vaginata are distributed in Asia. The taxonomic revision of S. vaginata and related species was performed by morphological comparison of leaves, strobili, and spores, and phylogenetic analysis. Based on these results, a new species, S. subvaginata, sp. nov., has been identified. Morphologically, S. subvaginata has intermediate form between S. vaginata and S. repanda, which differs mainly in its main stem being erect, dorsal leaves long-ciliolate on inner margin and outer margin denticulate or with 2-4 cilia at base (long-ciliolate on both inner and outer margins in S. vaginata, denticulate on both inner and outer margins in S. repanda), and acroscopic base of ventral leaves long ciliolate (sparsely long ciliolate in S. vaginata, short ciliolate to denticulate in S. repanda). Moreover, phylogenetic analysis using three chloroplast markers(rbcL, atpI, and psbA) revealed that S. subvaginata is a distinct species among the anisosporophyllous species clade in Selaginellaceae.

Synthesis and Polymerization of Methacryloyl-PEG-Sulfonic Acid as a Functional Macromer for Biocompatible Polymeric Surfaces

  • Kim, Jun-Guk;Sim, Sang-Jun;Kim, Ji-Heung;Kim, Soo-Hyun;Kim, Young-Ha
    • Macromolecular Research
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    • 제12권4호
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    • pp.379-383
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    • 2004
  • Poly(ethylene glycol)s (PEGs) are unique in their material properties, such as biocompatibility, non-toxicity, and water-solublizing ability, which are extremely useful for a variety of biomedical applications. In addition, a variety of functional PEGs with specific functionality at one or both chain ends have been synthesized for many specialized applications. Surface modifications using PEG have been demonstrated to decrease protein adsorption and platelet or cell adhesion on biomaterials. Furthermore, PEGs having anionic sulfonate terminal units have been proven to enhance the blood compatibility of materials, which has been demonstrated by the negative cilia concept. The preparation of telechelic PEGs having a sulfonic acid group at one end and a polymerizable methacryloyl group at the other is an interesting undertaking for providing macromers that can be used in various vinyl copolymerization and gel systems. In this paper, preliminary results on the synthesis and polymerization behavior of a novel PEG macromer is described with the aim of identifying a biocompatible material for applications in various blood-contacting devices.

In vitro Nasal Cell Culture Systems for Drug Transport Studies

  • Cho, Hyun-Jong;Termsarasab, Ubonvan;Kim, Jung-Sun;Kim, Dae-Duk
    • Journal of Pharmaceutical Investigation
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    • 제40권6호
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    • pp.321-332
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    • 2010
  • Growing interest in the nasal route as a drug delivery system calls for a reliable in vitro model which is crucial for efficiently evaluating drug transport through the nasal cells. Various in vitro cell culture systems has thus been developed to displace the ex vivo excised nasal tissue and in vivo animal models. Due to species difference, results from animal studies are not sufficient for estimating the drug absorption kinetics in humans. However, the difficulty in obtaining reliable human tissue source limits the use of primary culture of human nasal epithelial cells. This shortage of human nasal tissue has therefore prompted studies on the "passage" culture of nasal epithelial cells. A serially passaged primary human nasal epithelial cell monolayer system developed by the air-liquid interface (ALI) culture is known to promote the differentiation of cilia and mucin gene and maintain high TEER values. Recent studies on the in vitro nasal cell culture systems for drug transport studies are reviewed in this article.

Microscopic research on the olfactory organ of the Far Eastern brook lamprey Lethenteron reissneri (Pisces, Petromyzontidae)

  • Hyun-Tae Kim;Jong-Young Park
    • Applied Microscopy
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    • 제50권
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    • pp.18.1-18.7
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    • 2020
  • The olfactory anatomy and histology of Lethenteron reissneri were researched using a stereo microscope, a light microscope, and a scanning electron microscope. As in other lampreys, it shows same characters as follows: i) a single olfactory organ, ii) a single tubular nostril, iii) a single olfactory chamber with gourd-like form, iv) a nasal valve, v) a nasopharyngeal pouch, vi) a sensory epithelium (SE) of continuous distribution, vii) a supporting cells with numerous long cilia, viii) an accessory olfactory organ. However, the description of a pseudostratified columnar layer in the SE and Non SE is a first record, not reported in sea lamprey Petromyzon marinus. In particular, both 19 to 20 lamellae in number and olfactory receptor neuron's quarter ciliary length of the knob diameter differ from those of P. marinus. From these results, it might be considered that the olfactory organ of L. reissneri shows well adaptive structure of a primitive fish to slow flowing water with gravel, pebbles, and sand and a hiding habit into sand bottom at daytime. The lamellar number and neuron's ciliary length may be a meaningful taxonomic character for the class Petromyzonida.

알레르기 비염 유발 생쥐에 대한 형개연교탕(荊芥連翹湯)의 iNOS 생성 억제 효과 (The Effects of Hyunggaeyungyo-tang of Suppression of iNOS Production on Mice with Allergic Rhinitis)

  • 박정훈;홍승욱
    • 한방안이비인후피부과학회지
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    • 제25권1호
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    • pp.12-21
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    • 2012
  • Background and Objectives : Allergic rhinitis is one of the most common diseases in the otorhinolaryngology area. in oriental clinic, Hyunggaeyungyo-tang(HYT) has been used as a primary prescription to treat allergic rhinitis. However, there have been no studies so far performed on the effect of this HYT use. The purpose of this study was find out therapeutic effects of its exclusive use on the rat with allergic rhinitis. Material and Methods : Thirty BALB/c mice were divided into three group : normal group(NOR), control group(CON) inoculated with allergic rhinitis and sample group(SAM) treated with the HYT extract after it was treated the same as the control group. Rats were sensitized intraperitoneally with ovalbumin solution 4times at intervals of 2 days. After that time, rats in SAM were administered by HYT to treat the inflammation. Results : 1. The number of eosinophil in SAM noticeably decreased than CON and this decrease had probability. The inhibition of eosinophil distribution. The infiltration of eosinophil in SAM noticeably decreased than CON. 2. The damaged mucosa as disruption of cilia in respiratory cell, vacant mucose secreting cell and infiltration of inflammation intricate cells in CON were increased than NOR, but SAM same as normal configuration. Decrease of icthing and sneezing intricate neurotransmitter (substance P). Decrease of angiogenesis intricate cytokine(MIP-2). 3. Transcription factor(NF-${\kappa}B$ p65) was decreased. 4. Transcription factor inhibitor(p-$I{\kappa}B$) was decreased. 5. Inflammation cytokine(iNOS) was decreased. Conclusion : The results suggest that HYT is significantly effective in the treatment of inflammation caused by allergic rhinitis through the suppression of NF-${\kappa}B$ activation and iNOS production.

냉동보존이 진주조개(Pinctada fucata martensii) 유생의 미세구조에 미치는 영향 (The Effects of Cryopreservation on Fine Structures of Pearl Oyster(Pinctada fucata martensii) Larvae)

  • 최윤희;조필규;김태익;배승철;장영진
    • 한국발생생물학회지:발생과생식
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    • 제11권2호
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    • pp.79-84
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    • 2007
  • 냉동보존이 진주조개(Pincata fucata martensii) 유생의 형태 및 구조에 미치는 영향을 알아보기 위하여 냉동전후 D형 및 각정기 유생을 광학 및 전자현미경으로 조사하였다. 동해방지제는 0.2 M sucrose를 첨가한 2.0 M $Me_2SO$를 사용하였다. 냉동후 유생은 일부 패각이 손상되긴 했지만 hinge와 prodissoconch가 뚜렷하게 나타났으며, 소포체, 지질 과립, 미토콘드리아, 핵 등을 포함한 세포내 소기관들이 고르게 분포되어 있었다. 또한, 섬모가 규칙적으로 배열되어 있었고, 섬모 아래 미토콘드리아와 지질과립이 위치해 있는 것이 관찰되었으나, 일부 해동된 유생에서 섬모의 불규칙적인 배열과 섬모환이 둥글게 뭉쳐져 있는 모습이 관찰되었다. 이러한 결과는 진주조개의 D형 유생과 각정기 유생이 냉동에 쉽게 영향을 받을 수 있다는 것을 보여준다. 따라서 냉동보존 시 세포의 손상을 감소시킬 수 있는 연구가 이루어져야 할 것으로 사료된다.

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한국산 미기록 이끼토양자라하모충(섬모충문: 다막충강: 하모충목)의 재기재와 유사종의 비교 (Redescription of Newly Recorded Ciliate, Euplotes muscorum(Ciliophora: Polyhymenophora: Hypotrichida) and Comparison with Related Species from Korea)

  • Jong O Jo;Mann Kyoon Shin
    • Animal Systematics, Evolution and Diversity
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    • 제19권2호
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    • pp.227-235
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    • 2003
  • 2002년 제주도의 한 웅덩이에서 채집된 섬모충류를 실험실에서 분리, 배양한 결과 하모류의 이끼토양자라하모충(Euplotes muscorum Dragesco, 1970)으로 동정되었으며 한국에서 처음으로 보고되어 재기재한다. 기재는 생체 표본 관찰, protargol impregnation 그리고 형태통계에 기초하여 기술하였다. 이 종의 형태적 특징은 몸길이 63-78${\mu}m$, 폭 40-52${\mu}m$, 9개의 전방복극모, 5개의 후방극모, 4개의 미극모, 한 개의 대핵과 소핵, 몸길이의 약 2/3을 덮고 있는 막판구대는 32-35개의 구부막판으로 이루어졌고, 8개의 등쪽섬모열, 중앙의 등쪽섬모열의 섬모는 20-24개, 등쪽섬모배열은 complex type 등의 특징들을 가진다. 이 종은 이끼자라하모충(E. muscicola Kahl,1932)과 매우 비슷하지만 이끼토양자라하모충은 8개의 등쪽섬모열, complex type의 등쪽섬모배열을 가지는데 반해 이끼자라하모충는 9개의 등쪽섬모열, multiple type의 등쪽섬모배열을 가지는 형태적 특징을 각각 나타낸다.

Metagonimus yokogawai 세르카리아의 감각유두에 관한 연구 (Study on Sensory Papillae of Metagonimus yokogawai Cercaria)

  • 김재진;민득영소진탁
    • Parasites, Hosts and Diseases
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    • 제22권1호
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    • pp.11-20
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    • 1984
  • A number of studies on the papillae of cercariae of trematodes reported that the papillar patterns (or chaetotaxy) of cercariae might be an excellent method to attain better understanding of the digenetic trematodes (Richard, 1971 ; Short and Cartrett, 1973; Bayssade-Dufour, 1979) . The present study was aimed to determine the number, distribution pattern and structure of the sensory papillae of Metagonimus yokogawai cercariae, and to elucidate the chaetotaxy of this digenetic trematode. M. yokogawai cercariae were pipetted from a vial in which infected snails (Semisulcospira libertina) had been kept for 3 hours. The snails were collected from an endemic area of M. yokogawai, Boseong river in west-southern part of Korea. Observations of papillae were based on light microscopy of those stained with silver nitrate, and on scanning electron microscopy The results are summarized as follows: 1, All papillae observed were uniciliated. 2. Cilia in anterior tip were shorter than the others in other portions. 3. The body papillae were arranged in essentially symmetrical patterns, Total number of the papillae was 126(63 pairs) in average; anterior tip 40(20 pairs), ventral 20(10 pairs), lateral 42(21 pairs), and caudal 8(4 pairs). 4. The chaetotany of M. yokogawai cercaria was: Ci cycle ($3+3C_{I}V,{\;}2+2C_{I}L,{\;}2+3C_{I}D),{\;}C_{II}{\;}cycle(2C_{II}V,{\;}1C_{II}L,{\;}2C_{II}D),{\;}C_{lll}{\;}cycle{\;}(1+lC_{III}V,{\;}1C_{IlI}L),{\;}C_{IV}{\;}cycle{\;}(1C_{IV}V,{\;}IC_{lV}L){\;}in{\;}cephalic{\;}region:{\;}A_I(1A_{IV}V,{\;}1+2A_{I}L,{\;}1A_{I}D),{\;}A_{II}(1A_{II}V,{\;}1+3A_{II}L,{\;}1A_{II}D),{\;}A_{III}(1A_{III}V,{\;}1+1A_{III}L,{\;}1A_{III}D){\;}and{\;}A_{IV}(1A_{IV}V,{\;}2A_{IV}L)$ in antacetabular region: $1M_{I}V{\;}and{\;}2M_{I}L$ in median: $1+1P_{I}L,{\;}1P_{II}L,{\;}1P_{II}D,{\;}1P_{III}L,{\;}1P_{IV}L{\;}and{\;}1P_{IV}D$ in postacetabular region: 2-2-2-2 in caudal region.

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