Development and Reproduction (한국발생생물학회지:발생과생식)
- Volume 11 Issue 2
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- Pages.79-84
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- 2007
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- 2465-9525(pISSN)
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- 2465-9541(eISSN)
The Effects of Cryopreservation on Fine Structures of Pearl Oyster(Pinctada fucata martensii) Larvae
냉동보존이 진주조개(Pinctada fucata martensii) 유생의 미세구조에 미치는 영향
- Choi, Youn-Hee (Institute of Fisheries Sciences, Pukyong National University) ;
- Jo, Pil-Gue (Division of Marine Environmental & Bioscience, Korea Maritime University) ;
- Kim, Tae-Ik (National Fisheries Research and Development Institute) ;
- Bai, Sung-Chul C. (Department of Aquaculture, Pukyong National University) ;
- Chang, Young-Jin (Department of Aquaculture, Pukyong National University)
- 최윤희 (부경대학교 수산과학연구소) ;
- 조필규 (한국해양대학교 해양환경.생명과학부) ;
- 김태익 (국립수산과학원) ;
- 배승철 (부경대학교 양식학과) ;
- 장영진 (부경대학교 양식학과)
- Published : 2007.09.30
Abstract
The freezing susceptibilities of two larval stages (D-shaped and umbo) of the pearl oyster (Pinctada fucata martensii) were evaluated by the electron microscopy (light, transmission electron and scanning electron). The morphological shapes were examined from each pre-frozen or frozen-thawed stage of the cryopreserved larvae in liquid nitrogen by using the cryoprotectant, dimethyl sulfoxide (
냉동보존이 진주조개(Pincata fucata martensii) 유생의 형태 및 구조에 미치는 영향을 알아보기 위하여 냉동전후 D형 및 각정기 유생을 광학 및 전자현미경으로 조사하였다. 동해방지제는 0.2 M sucrose를 첨가한 2.0 M