• 생약학회지
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• 제29권2호
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• pp.67-70
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• 1998

Two sesquiterpene lactones were isolated from the flowers of Chrysanthemum boreale Makino by the silica gel column chromatography and recrystallization. On the basis of spectrometric studies including $^1H-NMR,\;^{13}C-NMR,\;DEPT,\;^1H-^1H\;COSY,\;{13}C-^1H\;COSY$, IR and Mass, compounds 1 and 2 were identified as germacranolide, tulipinolide and costunolide, respectively. And they showed antibacterial activity against Vibrio parahaemolyticus, Bacillus subtilis, Bacillus cereus and Staphylococcus aureus. This is the first report that Chrysanthemum boreale contained tulipinolide and costunolide.

• 한국환경과학회지
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• 제26권11호
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• pp.1285-1295
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• 2017

Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-${\small{O}$-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy-8-${\small{O}$-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an $IC_{50}$ value of $7{\mu}g/mL$, whereas compound 1 did not exhibit significant activity with an $IC_{50}$ value of over $14{\mu}g/mL$ against murine macrophage.

• 생약학회지
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• 제27권3호
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• pp.207-211
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• 1996

Cumambrin A has been isolated from the dried flowers of Chrysanthemum boreale Makino. The complete $^1H$ and $^{13}C$ NMR assignment of cumambrin A was achieved from two-dimensional $^1H$-$^1H$ COSY and $^{13}C$-$^1H$ COSY spectra with the aid of homonuclear and heteronuclear double resonance experiments. The its structure has been verified by single crystal X-ray diffraction.

• Food Science and Biotechnology
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• 제14권3호
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• pp.350-354
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• 2005

The effect of the essential oil obtained from Chrysanthemum boreale Makino on the apoptosis of KB cells was investigated. Cytotoxicity and cellular DNA content were analyzed by MTT assay, flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. The various cytotoxic effects of the essential oil which are hallmarks of apoptosis, including DNA fragmentation, apoptotic body formation, and sub-G1 DNA content, all progressed in a dose-dependent manner. Treatment with an apoptosis-inducing concentration of the essential oil caused rapid and transient induction of caspase 3 activity. Further, the efficacious induction of PARP cleavage and caspase-3 activation was observed at an essential oil concentration of 0.1 and 0.2 mg/mL for 12 hr.

• 한국환경과학회지
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• 제28권11호
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• pp.993-1004
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• 2019

Chrysanthemum boreale Makino (C. boreale) is widely distributed in Asian countries, and has traditionally been used to treat various inflammatory diseases including bronchitis. In this study, we aimed to isolate biologically active compounds from leaves and stems of C. boreale. Chemical components were purified by column chromatograpy and recyclic HPLC, and characterized from their spectral data (IR, MS, NMR). Biological activity experiments were conducted for Farnesyl-protein transferase (FPTase) activity, apoptosis and nitirc oxide (NO) release. As a results, three sesquiterpene lactones were isolated. Compound 1 (4-methoxy-8-O-acetyl-10-hydroxy-2,11(13)-guaiadiene-12,6-olide) showed strong cytotoxic activities having an average growth inhibition of 50% ($GI_{50}$) value of $1.89{\mu}g/m{\ell}$ against human colon adenocarcinoma cells. Compound 1 also showed a low half maximal inhibitory concentration ($IC_{50}$) value of $10{\mu}g/m{\ell}$ for NO release. In the caspase 3 activity, compound 1 and compound 2 (8-O-(2-carbonyl-2-butyl)-3,10-dihydroxy-4,11(13) -guaiadiene-12,6-olide) exhibited 94% and 90% apoptosis inhibition activity, respectively. Compound 3 (4,8-O-diacetyl -10-hydroxy-2(3),11(13)-guaiadiene-12,6-olide) showed a strong inhibitory effect on FPTase activity with 90% inhibitory activity at a concentration of $100{\mu}g/m{\ell}$. These results clearly show the presence of lactone compounds in the leaves and stems, which may partially contribute to the pharmacological activity of C. boreale.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.175.1-175
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• 1995

No Abstract, See Full Text

• 한국약용작물학회지
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• 제13권5호
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• pp.284-287
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• 2005

The flowers of Chrysanthemum boreale Makino were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH and $H_2O$. Two compounds from the n-hexane fraction and one glucoside from the n-BuOH fraction were isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\beta}-sitosterol$ (1), phytol (2) and zingerone $4-O-{\beta}-D-glucopyranoside$ (3). Compounds 2 and 3 were isolated for the first time from this plant.

• 한국식품조리과학회지
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• 제22권6호통권96호
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• pp.768-773
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• 2006

유럽의 허브요법을 대표하는 캐모마일차와 국내산 국화차에 속하는 감국차의 휘발성성분을 동시증류추출법에 의해 추출하여 GC 및 GC-MS로 분석${\cdot}$동정하고 비교하였다. 캐모마일차의 휘발성성분으로는 cubebene(14.59%), ${\beta}$-elemene(4.88%), ${\delta}$-cadinol(1.54%)을 포함한 총 46종류의 화합물이 동정되었다. 반면, 국내산 국화차에서는 santalol(6.25%), bornyl acetate(3.47%), farnesene(3.37%), 1,8-nonadiene(2.80%), caryophyllene oxide(2.77%) 및 thymol(2.16%) 등 총 45종류의 화합물이 동정 또는 추정되었다. 동정 또는 추정된 화합물 중 22종은 두 시료에 공통적으로 포함되어 있었다. 캐모마일차와 국화차에 공통으로 함유된 화합물 중 비교적 함량적으로 많이 들어있는 화합물은 4-terpineol, ${\alpha}$-terpineol, thymol, phenylacetaldehyde들이 었다.

• 한국약용작물학회지
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• 제11권1호
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• pp.1-4
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• 2003

천연물로부터 항산화 활성물질을 목적으로 DPPH 자유 라디칼 소거활성 검색법을 이용하여 검색을 실사하고, 유의한 활성을 나타낸 산국 메탄올 추출물로부터 2종의 화합물을 분리하고 기기분석을 이용하여 화합물 1과 2의 구조를 apigenin과 acacetin-7-O-rutinoside (Linarin)으로 동정하였다. 이들 화합물은 각각 13.3 및 $42.1{\mu}g$의 농도에서 DPPH 라디칼을 50% 소거하는 활성을 나타내었으며, 화합물 1은 양성 대조양물인 L-ascorbic acid $(RC_{50}\;=\;13.1{\mu}g)$에 비하여 강한 활성을 나타내었다.

• 한국식품영양과학회지
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• 제38권7호
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• pp.824-831
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• 2009

감국, 산국 및 구절초꽃 열수추출물에 덱스트린과 말토덱스트린 부형제의 첨가량을 달리하여 분말차를 제조하고 이의 품질특성과 관능특성을 평가하였다. 국화차 분말의 흡습성은 국화 품종별 덱스트린 단독 첨가구에서 가장 높았으며, 부형제의 종류 및 첨가량에 따라 유의적인 차이를 보였다. 용해도는 덱스트린과 말토덱스트린을 8:2로 혼합한 처리구에서 당도 변화가 크게 나타났다. 국화 분말차의 색도는 부형제에 따른 차이는 없었으나, 덱스트린과 말토덱스트린을 9:1로 혼합한 첨가구에서 명도를 나타내는 L값이 높게 나타났으며, 국화 분말차의 pH는 $5.33{\sim}5.63$ 범위를 보였다. 갈변도와 탁도는 품종, 부형제의 종류 및 농도에 따라 유의적인 차이를 나타내었으며, 환원당 함량은 국화 품종별로 덱스트린과 말토덱스트린을 8:2로 혼합한 처리구에서 높았다. 총폴리페놀 및 플라보노이드 함량과 총 항산화력은 산국 분말차가 각각 $6.47{\sim}6.75$, $1.95{\sim}1.99$ 및 $4.37{\sim}4.55\;mg/g$ 범위로 감국과 구절초 분말차보다 높았고 전자공여능은 감국 분말차가 $24.85{\sim}27.93%$로 산국과 구절초 분말차보다 높았다. 관능평가에서 색과 향은 유의적인 차이를 보이지 않았으며, 산국의 경우 쓴맛에 대한 낮은 평가로 인하여 전반적으로 기호도가 낮게 평가되었다.