• Title/Summary/Keyword: chrysanthemum

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Expression pattern of floral scent genes in different flowering stages of Chrysanthemum cultivars

  • Mekapogu, Maniulatha;Ahn, Myung Suk;Yoo, Jong Hee;Jeong, Jae Ah;Park, Jong Taek;Kwon, Oh Keun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.114-114
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    • 2018
  • Among the various volatile organic compounds (VOCs) emitted by the plant, floral scent plays a key role in attracting pollinators for reproduction and mediates ecological interactions. Floral scent is an important trait and industry drives the competition for flowers with novel scents. Chrysanthemum is one of the well-known ornamental plants and is a popular cut flower across the world. Floral scent and the genes responsible for the floral scent emission are poorly studied in chrysanthemum. In the present study, floral scent and the expression pattern of floral scent genes were analyzed in two chrysanthemum cultivars 'Golden Egg' and 'Gaya Glory'. Initially, intensity of the floral scent in five developing stages of flower including 'budding (B), bud developing (BD), initial blooming (IB), almost open (AO) and open flower (OF)' was analyzed using electronic nose (E-nose) with six metal oxide sensors. Based on the distance analysis, different stages of flower showed different relative intensity of scent according to the sensory evaluation. Although the scent pattern differed by stage, scent intensity was strongest in the OF stage in the completely opened flower in both the cultivars. Further, expression pattern of six genes in the floral scent pathway including FDS, IDI, ISPH, TPS2, TPS5 and TPS6 was observed in all the five stages of the flower in both the cultivars. The expression pattern of all the six genes differed by stage and the terpene synthase genes TPS2, TPS5 and TPS6 showed good expression levels in the $5^{th}$ flower stage compared to other stages. This study provides a preliminary data for understanding the regulation of floral scent in chrysanthemum.

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Antimicrobial Activities of Sesquiterpene Lactones Isolated from Hemisteptia lyrata, Chrysanthemum zawadskii and Chrysanthemum boreale (지칭개, 구절초 및 산국에서 분리한 Sesquiterpene lactones의 항균활성)

  • Jang, Dae-Sik;Park, Ki-Hun;Lee, Jong-Rok;Ha, Tae-Joung;Park, Yun-Bae;Nam, Sang-Hae;Yang, Min-Suk
    • Applied Biological Chemistry
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    • v.42 no.2
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    • pp.176-179
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    • 1999
  • In the bioactivity test of nine sesquiterpene lactons isolated from Hemisteptia lyrata, Chrysanthemum zawadskii and Chrysanthemum boreale, we investigated their antimicrobial activities against ten bacteria and six phytopathogenic fungi. Hemistepsin A and hemistepsin B isolated from H. lyrata and tulipinolide isolated from C. boreale exhibited strong antibacterial activity. In the antifungal test, hemistepsin B, angeloylcumambrin B, tigloylcumambrin B and costunolide showed broad activity. Both antibacterial and antifungal experiments exhibited quite clear specificity against microbial strains.

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A Study on Anti-oxidative, Anti-inflammatory, and Melanin Inhibitory Effects of Chrysanthemum Sibiricum Extract (구절초 꽃 추출물의 항산화, 항염증 및 멜라닌 생성 억제 효과에 관한 연구)

  • You, Seon-hee;Moon, Ji-sun
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.4
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    • pp.762-770
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    • 2016
  • The purpose of this study is to observe how Chrysanthemum Sibiricum Extract has anti-oxidant activity, cytotoxicity for skin cells, and anti-inflammatory and melanin inhibitory effects in order to find the development possibility of Chrysanthemum Sibiricum Extract as the ingredient of a functional cosmetic product. According to the analysis, Chrysanthemum Sibiricum Extract was found to have high contents of polyphenols and flavonoids and excellent DPPH radical scavenging activity. The extract had no significant cytotoxicity for Raw 264.7 cell and B16F10 cell and significantly inhibited the creation of NO induced LPS in Raw 264.7 cell so as to present anti-inflammatory effect. In B16F10 cell, melanin creation was induced with ${\alpha}$-MSH, and then melanin biosynthesis inhibition was measured. As a result, melanin creation was inhibited in the concentration dependence way. Given the results, it is considered that Chrysanthemum Sibiricum Extract is applicable as the ingredient of a functional cosmetic product that has low cytotoxicity for skin cells, high anti-oxidant activity, anti-inflammatory effect, and whitening effect.

A Study on the Perception and Consumption Behavior of Blended Tea and Siberian Chrysanthemum - Focusing on female college students in their 20's - (블렌딩티와 구절초에 대한 인식과 소비행동 연구 - 20대 여대생을 중심으로 -)

  • Lee, Ji-O;Na, Yeseul;Oh, Ji-eun;Cho, Mi Sook
    • Journal of the Korean Society of Food Culture
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    • v.36 no.3
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    • pp.300-307
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    • 2021
  • In this study, consumers' perception and consumption behavior of blended tea were investigated. Also, consumer perception of Siberian chrysanthemum, consumption behavior, and awareness of efficacy were investigated. As a result of the survey, it was confirmed that it was necessary to develop a blended tea with taste and flavor characteristics that consumers prefer, having the capability of relieving stress, and preventing cold or respiratory disease. In addition, the degree of recognition of Siberian chrysanthemum was very low, and the recognition of the efficacy appeared in the order of 'cold relief' and 'respiratory disease relief'. Factor analysis results, safety attributes, health attributes, product attributes, marketing attributes, and sensory attributes were factorized into five factors, and after analysis, it was concluded that the sensory attributes of taste and aftertaste were considered the most important. Consumers surveyed appeared to consume blended tea for 'relieving mood or stress relief', to enjoy the taste, and to prevent cold or respiratory diseases. Siberian chrysanthemum displays efficacy in cold relief and relief of respiratory diseases. Therefore, developing Siberian chrysanthemum as a blended tea could be a practical application that can expand the range of its use, making it more popular and increase its commercial applications.

Improvement of In Vitro Seed Germination in Chicory (Cichorium intybus L.) and Garland Chrysanthemum (Chrysanthemum coronarium L.) (치커리(Cichorium intybus L.)와 쑥갓(Chrysanthemum coronarium L.)의 기내 종자발아 개선)

  • Hwang, Hyeon-Jeong;Choi, Geun-Won
    • Journal of Bio-Environment Control
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    • v.17 no.4
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    • pp.297-305
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    • 2008
  • A series of experiments were conducted to suggest efficient in vitro germination conditions to improve germination rate and aseptic seedling production in chicory and garland chrysanthemum. For this purpose, various levels of NaOCl concentration and soaking treatment time combinations were tasted. Fifteen minutes of soaking treatment with 5% NaOCl solution significantly improved germination and seedling formation percentages and prevented contamination in both chicory 'Precole' and 'Chiavari' and garland chrysanthemum 'Okiku 3'. There was no significant difference in germination response between MS and 1/2 MS medium compositions, but germination and seedling formation were delayed as sucrose concentration increased. Although petri-dish among various culture containers gave rise to improved germination responses in chicory 'Precole', there was no significant difference in germination responses among culture container types in chicory 'Chiavari' and garland chrysanthemum 'Okiku 3'. The ultrasonic treatment stimulated germination and seedling formation in both chicory cultivars but there was no effect in garland chrysanthemum 'Okiku 3'. The results suggested 15 minutes with 5% NaOCl for seed sterilization, 1/2 MS medium with no sucrose and petri-dish as proper medium composition and culture container, and 120 minutes with 5% NaOCl solution of ultrasonic treatment as promoting method for in vitro germination in chicory and garland chrysanthemum.

Antioxidant Effect of Extracts Obtained from Three Chrysanthemum Species (국화과 Chrysanthemum속 식물 3종의 항산화 효과)

  • Woo, Jeong-Hyang;Shin, So-Lim;Jeong, Heon-Sang;Lee, Cheol-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.4
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    • pp.631-636
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    • 2010
  • To develop a natural antioxidant from three Chrysanthemum species, flower and shoot extracts of Chrysanthemum frutescens, Chrysanthemum morifolium and Chrysanthemum zawadskii ssp. naktongense were obtained and their phenolic compound contents, scavenging effects on DPPH and ABTS radicals, ferrous ion chelating effects and inhibition activity on lipid peroxidation of linoleic acid were studied. Shoots of C. morifolium showed the highest levels in all above mentioned analyses. Especially, shoot extract of C. morifolium had high scavenging activities on ABTS radicals, similar to ascorbic acid or BHT. Ferrous ion chelating effect was the lowest in a C. morifolium shoot extract, but the highest in a C. morifolium flower extract. Inhibition activity on lipid peroxidation of linoleic acid was the highest with C. frutescens and C. morifolium shoots, but activity was lower than BHT. From present study, a shoot extract of C. morifolium is demonstrated as a valuable source for the development of a natural antioxidant. However, due to its low levels of ferrous ion chelating effects and inhibition activity on lipid peroxidation, a combination of other antioxidants with C. morifolium extract is recommended for the development of a new antioxidant.

Analysis and Improvement Plan of Cultivation and Postharvest Management Status of Cut Chrysanthemum Farms in Korea (국내 절화국화 재배농가의 재배와 수확 후 관리현황 분석 및 개선 방안)

  • Roh, Yong Seung;Yoo, Yong Kweon
    • FLOWER RESEARCH JOURNAL
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    • v.26 no.4
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    • pp.187-194
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    • 2018
  • This study was conducted to investigate the facilities, cultivation, postharvest management, and distribution status of 27 cut chrysanthemum farms in Korea. The 60% of farms have cultivated the cut chrysanthemum using soil fertigation system in the PE plastic house. In Jeonnam and Busan provinces, Standard type of chrysanthemum was cultivated mainly than spray type of chrysanthemumJeoas. Most farms have been producing the rooted cuttings by plug system using cuttings self-propagated or purchased from the company, but farms in Jeonnam have been planting cuttings directly on cultivation bed. And the 66.6% of cut chrysanthemum farms have been pretreating with dipping in hot water or tap water after harvesting. Precooling was not performed on 70.4% of the farms, and precooling farms have been mainly conducted at temperature of $2-4^{\circ}C$. After harvesting, 70.4% of the farms stored the cut flowers at $2-4^{\circ}C$ for more than 48 hours to control the distribution volume. Cut chrysanthemum was graded mainly by individuals before distribution, and some export farmers have been conducting the cooperative grading. In distribution, all farms have distributed the cut flowers to the domestic markets, and 44.4% of these farms have been also exporting. The 63.0% of farms distributed to domestic market have been trading with flower auction sites.

Control Effects of Frankliniella occidentalis by using Trap Plants and Orius laevigatus in Chrysanthemum PVC House (시설국화에서 트랩식물과 미끌애꽃노린재를 이용한 꽃노랑총채벌레 방제)

  • Choi, Yong-Seok;Whang, In-Su;Park, Deog-Kee;Lee, Jun-Seok;Ham, Eun-Hye
    • The Korean Journal of Pesticide Science
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    • v.17 no.4
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    • pp.440-447
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    • 2013
  • F. occidentalis (WFT, western flower thrips) is a major pest in artificial chrysanthemum houses. Nnumber of WFT attracted to yellow sticky trap was highest at trap plant and lowest at 15 and 20m places away from trap plant. Number of WFT attracted to yellow stick trap when trap plant was placed in chrysanthemum house 30 days after planting (resident WFT) was 4.4~7.7 times more than at 5 and 10 m places away from trap plant and when trap plant was placed in chrysanthemum house immediately after planting (resident WFT) was 5.7~9.4 times more at 5 and 10 m places away from trap plant. Number of WFT between the place trap plant located and unlocated was undifferent when cultured chrysanthemum formed flower. In case of the plot that trap plant and natural enemy was used simultaneously, number of WFT was highter then chemicals plot. In case of the plot that trap plant and chamicals (chemicals was sprayed on trap plant only) WFT was controlled in trap plant place only but density of WFT on 5m and 10m places away from trap plant was increased. Therefore, WFT could be controlled effectively by use of trap plant (flowering yellow chrysanthemum) and natural enemy simultaneously.