• Title/Summary/Keyword: chromosome 15

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Cytological Study of Euphaedusa fusaniana (Stylommataphora: Clausiliidae) of Korea

  • Park, Gab-Man
    • The Korean Journal of Malacology
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    • v.23 no.1
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    • pp.59-63
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    • 2007
  • The chromosome numbers and karyotype of Euphaedusa fusaniana, a kind of Korean land snail are investigated here. From E. fusaniana, chromosome numbers of 28 (n) and 56 (2n) were counted. Euphaedusa fusaniana has 15 pairs of metacentric chromosomes and 13 pairs of submetacentric chromosomes. The mean total length of all chromosomes was $128.7\;{\pm}\;9.93\;{\mu}m$. The maximum length of chromosomes No. 1 was $9.6\;{\pm}\;0.21\;{\mu}m$.

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Bootstrap Analysis of ILSTS035 Microsatellite Locus in Hanwoo Chromosome 6

  • Lee, Jea-Young;Lee, Yong-Won;Kim, Mun-Jung
    • Journal of the Korean Data and Information Science Society
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    • v.15 no.1
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    • pp.75-81
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    • 2004
  • We selected, in previous research, a major DNA Marker 235bp of ILSTS035 microsatellite locus in progeny test Hanwoo chromosome 6. We apply a major DNA Marker 235bp to perormance valuation Hanwoo chomosome 6. We use bootstrap BCa method and calculate confidence interval. A major DNA Marker 235bp is verified that it does not have environmental effect but affects primely economic trait factor.

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Investigation of Single Nucleotide Polymorphisms in Porcine Chromosome 2 Quantitative Trait Loci for Meat Quality Traits

  • Do, K.T.;Ha, Y.;Mote, B.E.;Rothschild, M.F.;Choi, B.H.;Lee, S.S.;Kim, T.H.;Cho, B.W.;Kim, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.2
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    • pp.155-160
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    • 2008
  • Several studies have reported quantitative trait loci (QTL) for meat quality on porcine chromosome 2 (http://www.animalgenome.org/QTLdb/pig.html). For application of the molecular genetic information to the pig industry through marker-assisted selection, single nucleotide polymorphism (SNP) markers were analyzed by comparative re-sequencing of polymerase chain reaction (PCR) products of 13 candidate genes with DNA from commercial pig breeds such as Berkshire, Yorkshire, Landrace, Duroc and Korean Native pig. A total of 34 SNPs were identified in 15 PCR products producing an average of one SNP in every 253 bp. PCR restriction fragment length polymorphism (RFLP) assays were developed for 11 SNPs and used to investigate allele frequencies in five commercial pig breeds in Korea. Eight of the SNPs appear to be fixed in at least one of the five pig breeds, which indicates that different selection among pig breeds might be applied to these SNPs. Polymorphisms detected in the PTH, CSF2 and FOLR genes were chosen to genotype a Berkshire-Yorkshire pig breed reference family for linkage and association analyses. Using linkage analysis, PTH and CSF2 loci were mapped to pig chromosome 2, while FOLR was mapped to pig chromosome 9. Association analyses between SNPs in the PTH, CSF2 and FOLR suggested that the CSF2 MboII polymorphism was significantly associated with several pork quality traits in the Berkshire and Yorkshire crossed F2 pigs. Our current findings provide useful SNP marker information to fine map QTL regions on pig chromosome 2 and to clarify the relevance of SNP and quantitative traits in commercial pig populations.

Cytogenetic Study of Pleuronectes obscurus, Konosirus punctatus and Pseudoblennius percoides

  • Kim, Eun-Mi;An, Hye-Sook;Park, In-Seok
    • Fisheries and Aquatic Sciences
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    • v.10 no.1
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    • pp.24-29
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    • 2007
  • Our objective was to clarify the cytogenetic characteristics, including karyotypes, cellular DNA content, and nuclear size of erythrocytes, of black plaice Pleuronectes obscurus, dotted gizzard shad Konosirus punctatus, and perch sculpin Pseudoblennius percoides, collected from the coastal areas of Jo Island, Busan, Korea. Karyotypes of P. obscurus and K. punctatus both had a diploid number of 48 and a fundamental number (FN) of 48, with a chromosome formula of 48T. The karyotype of p. percoides had a diploid number of 46 and FN of 56, with a chromosome formula of 10SM +36T. No sex-associated heteromorphic pairs were detected for any species. The variation in DNA values (P. obscurus=1.15 pg/nucleus, K. punctatus=1.56pg/nucleus, P. percoides=1.11 pg/nucleus) was positively related to variation in chromosome FN.

Chromosome Counts from the Flora of Korea with Emphasis on Apiaceae

  • Sun, Byung-Yun;Park, Jung-Hee;Kwak, Min-Ju;Kim, Chul-Hwan;Kim, Kyung-Sik
    • Journal of Plant Biology
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    • v.39 no.1
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    • pp.15-22
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    • 1996
  • Seventy-three chromosome counts are reported from populations in 70 species from Korea, of which 34 counts in 32 species belong to Apiaceae. New species counts are in Acanthopanax chiisanensis(2n=48), Hydrocotyle japonica(2n=ca.96), H. maritima (n=12), Libanotis coreana (2n=22), Lindera sericea (n=12), Quercus myrsinaefolia (n=12), Rhamnus yoshinoi (n=12), Salix hulteni (n=19), Symplocos chinensis for. pilosa (n=11) and Vaccinium oldhami (n=12). New chromosome level for Asperula odorata (n=11), Cryptotaenia japonica (2n=16) and Sium ninsi (2n=18) is also provided. The taxonomic implications of certain of these counts are discussed.

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A Major DNA Marker of BM4311 Microsatellite Locus in Hanwoo Chromosome 6 using the Bootstrap BCa Method

  • Lee, Jea-Young;Kim, Mun-Jung;Lee, Young-Won
    • Journal of the Korean Data and Information Science Society
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    • v.15 no.1
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    • pp.41-47
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    • 2004
  • DNA marker 95bp and 100bp are selected as major DNA markers of the BM4311 microsatellite locus in progeny test Hanwoo chromosome 6 linkage map. This document is tried to know whether DNA marker 95bp and 100bp are also major DNA markers in Hanwoo performance valuation in chromosome 6 linkage map. The bootstrap BCa method will be used to calculate confidence interval for DNA markers.

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Studies on the Chromosome of the Korean Mouse (한국산 생쥐의 염색체에 관한 연구)

  • 김강련;장남섭;이석우
    • The Korean Journal of Zoology
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    • v.15 no.1
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    • pp.35-38
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    • 1972
  • Studies on the chromosome of Mus musculus have been done by Galton in 1965. In the present studies the authors have analyzed the chromosome pattern of Mus molossinus and found the same pattern of karyotype as observed by Galton. In addition, it was found that there exists no difference in karyotype between different localities and between different species.

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Cytogenetic Studies of 384 Couples with Recurrent Abortion (반복유산을 경험한 384부부의 세포유전학적 연구)

  • Choi, Soo-Kyung;Min, Eung-Ki;Roh, Sung-Il;Paik, Yong-Kyun;Lyu, Myung-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.18 no.2
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    • pp.223-231
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    • 1991
  • During the years 1984 to 1989, in order to determine of chromosome abnormalities are associated with recurrent spontaneous abortions, cytogenetic studies were performed 384 couples. Abnormal karyotypes were found in 51(13.3%) couples. There was no apparent relation with the number of abortions. The abnormalities were as follows: 17(4.4%) balanced translocation; 15(3.9%) mosaicisms; 17(4.4%) pericentric inversion; 2(0.5%) addition or isochromosome. Chromosome abnormalities were observed in 34(67%) of the wives and 17(33%) of the husbands. In addition, we detected polymorphic variants of chromosomes in 89(23.2%) subjects. Reciprocal translocations(13/17) were more common than the robertsonian type(4/17). All of the mosaicisms were associated with the sex chromosomes in 10 females and 5 males subjects. Pericentric inversions were most common in chromosome 9. Compared to previously studied general populations, significantly higher frequencies of translocations, mosaicisms and inversions were found in couples with repetitive spontaneous abortion. This suggests that couples should have chromosome studies after two or more abortions.

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A Study on the College Science Students' and Science Teachers' Understaning of Chromosome Behavior (대학생들과 과학교사들의 염색체 행동의 이해에 관한 연구)

  • Cho, Jung-Il;Kim, Kyoung-Joo
    • Journal of The Korean Association For Science Education
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    • v.13 no.2
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    • pp.219-229
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    • 1993
  • The purpose of this study is to investigate college science students' and science teachers' understanding of chromosomal behavior in the context of cell division. The research problems were as follows: 1. What is the level of college science students' understandings of chromosomal behaviors? 2. What is the level of science teachers' understandings of chromosomal behaviors? 3. What is the level of understanding by grade and major area? The sample consisted of 28 sophomore, 17 junior and 23 senior biology students; and 23 middle school science teachers and 14 high school biology teachers. The instrument of the study was a short answer required paper and pencil test. The results of the study were as follows: 1) About 15 percent of the sample could not count the number of chromosome in a cell in appropriate. 2) Seventy percent of the students, and 80 percent of the teachers identified homologous chromosomes as ones with the similar shape and size, and 30 percent of the whole sample could not pair two homologous chromosomes. 3) About 70 percent of the students and 30 percent of the teachers could not mark corresponding allele on chromosome. 4) Biology major students showed higher understanding of overall chromosomal behaviors than non Biology students. Based upon the results, some implications were made. The major one was a development of a teaching model in which students can improve the ability to connect chromosome theory to mendelian genetics.

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Hypersensitivity of Somatic Mutations and Mitotic Recombinations Induced by Mutagens in Transgenic Drosophila bearing Rat DNA Polymerase $\beta$ (Rat의 DNA Polymerase$\beta$ cDNA가 도입된 Transgenic Drosophila의 체세포 돌연변이 유발에 관한 연구)

  • 최영현;유미애;이원호
    • Environmental Mutagens and Carcinogens
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    • v.15 no.2
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    • pp.100-105
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    • 1995
  • The effects of DNA polymerase $\beta$ on the somatic chromosome mutations and mitotic recombinations were investigated using the transgenic Drosophila beating chimetic gene consisting of a promoter region of Drosophila actin 5C gene and rat DNA polymerase $\beta$. For detecting the somatic chromosome mutations and mitotic recombinations, the heterozygous (mwh/+) strains possessing or lacking transgene poi 13 were used. The spontaneous frequency of small mwh spots, due to deletion or nondisjunction etc., in the non-transgenic w strain and the transgenic p[pol $\beta$]-130 strain was 0.351 and 0.606, respectively. The spontaneous frequency (0.063) of large mwh spots, arises mostly from somatic recombination between the centromere and the locus mwh, in the transgenic p[pol $\beta$]-130 strain was about three times higher than that (0.021) of the non-transgenic w strain. The mutant clone frequencies of small and large mwh spots induced by N-methyl-N'-nitro-N-nitrosoguanidine and ethyl methanesulfonate in the transformant p[pol $\beta$]-130 were higher than those in the host strain w. The present results suggest that rat DNA polymerase $\beta$ participate at least in the somatic chromosome mutations and mitotic recombination processes.

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