Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Investigation of Single Nucleotide Polymorphisms in Porcine Chromosome 2 Quantitative Trait Loci for Meat Quality Traits

  • Do, K.T. (Department of Animal Science, Chungbuk National University) ;
  • Ha, Y. (Department of Animal Science, Chungbuk National University) ;
  • Mote, B.E. (Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University) ;
  • Rothschild, M.F. (Department of Animal Science, Center for Integrated Animal Genomics, Iowa State University) ;
  • Choi, B.H. (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Lee, S.S. (Animal Genetic Resouce Station, National Institute of Animal Science, RDA) ;
  • Kim, T.H. (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Cho, B.W. (College of Natural Resource and Life Sciences, Pusan National Univeristy) ;
  • Kim, K.S. (Department of Animal Science, Chungbuk National University)
  • Received : 2007.03.05
  • Accepted : 2007.07.23
  • Published : 2008.02.01
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Abstract

Several studies have reported quantitative trait loci (QTL) for meat quality on porcine chromosome 2 (http://www.animalgenome.org/QTLdb/pig.html). For application of the molecular genetic information to the pig industry through marker-assisted selection, single nucleotide polymorphism (SNP) markers were analyzed by comparative re-sequencing of polymerase chain reaction (PCR) products of 13 candidate genes with DNA from commercial pig breeds such as Berkshire, Yorkshire, Landrace, Duroc and Korean Native pig. A total of 34 SNPs were identified in 15 PCR products producing an average of one SNP in every 253 bp. PCR restriction fragment length polymorphism (RFLP) assays were developed for 11 SNPs and used to investigate allele frequencies in five commercial pig breeds in Korea. Eight of the SNPs appear to be fixed in at least one of the five pig breeds, which indicates that different selection among pig breeds might be applied to these SNPs. Polymorphisms detected in the PTH, CSF2 and FOLR genes were chosen to genotype a Berkshire-Yorkshire pig breed reference family for linkage and association analyses. Using linkage analysis, PTH and CSF2 loci were mapped to pig chromosome 2, while FOLR was mapped to pig chromosome 9. Association analyses between SNPs in the PTH, CSF2 and FOLR suggested that the CSF2 MboII polymorphism was significantly associated with several pork quality traits in the Berkshire and Yorkshire crossed F2 pigs. Our current findings provide useful SNP marker information to fine map QTL regions on pig chromosome 2 and to clarify the relevance of SNP and quantitative traits in commercial pig populations.

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References

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