• The Korean Journal of Food And Nutrition
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• v.9 no.4
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• pp.496-499
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• 1996

In order to develop the functional diet food, characteristics of chitosan and silk fibroin composite was designed. Chitosan extracted from a prawn and silk fibroin was prepared from silkworm. The silk fibroin was dissolved rapidly in the 8M LiBr at a temperature of more than 4$0^{\circ}C$. Amino acid composition of fibroin composite revealed the same pattern that of native silk fibroin and regenerated silk fibroin. Predominant amino acid of chitosan-fibroin composite contained glycine, alanine, serine, tyrosine, threonine, and glutamic acid in order. According to the basis on the infrared spectrum, chitosan-silk fibroin composite is not distinguished differents composite ratio of chitosan and silk fibroin.

• Macromolecular Research
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• v.12 no.5
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• pp.534-539
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• 2004

Two biopolymers, silk fibroin (SF) and chitosan, were conjugated by tyrosinase (EC 1.14.18.1), a polyphenolic oxidase, to improve their physicochemical properties, such as their thermal properties and morphological stabilities in organic solvents. The crosslinking between SF and chitosan took place mainly through Michael addition reactions. A main reaction between the amino groups in chitosan and o-quinone, the oxidation product of the tyrosyl residue in SF, was confirmed by UV spectroscopy. Measurements of viscosity and light scattering indicated that the crosslinked SF/chitosan conjugate was compact: it had a smaller particle size because of tight bonding forces between the SF and chitosan molecular chains. Thermal decomposition of SF/chitosan conjugates crosslinked by tyrosinase occurred at higher temperatures. The adhesiveness of the SF/chitosan conjugates decreased steadily as the crosslinking reaction progressed. We propose that this new crosslinking method be used for the preparation of silk fibroin/chitosan conjugates using tyrosinase. We expect that SF/chitosan conjugates crosslinked by tyrosinase can be used preferentially in biomedical applications because of its unique properties and non-toxicity.

• Polymer(Korea)
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• v.29 no.4
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• pp.408-412
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• 2005

Structure and characteristic of the films blended chitosan matrix with silk fibroin, extracted from Bombyx mori, were studied by X-ray diffraction, differential scanning calorimetry, FT-IR spectra analysis, SEM photographs, contact angle measurement and water absorbency in order to use as biomaterials. The blend films of $0\~30 wt\%$ fibroin content were prepared in acetic solution with $Li^+$ ion. It was found that the crystallinity of chitosan/fibroin blend films was decreased by the presence of intermolecular interactions such as hydrogen bonding between animo groups of chitosan and carbonyl groups of fibroin. As the proportion of fibroin in the blend increased, anhydrous crystalline phase of chitosan disappeared, and hydrated crystalline phase decreased, and $\beta$-structure crystalline phase of fibroin was formed. Therefore the blend films were crystallized into two different crystalline region of chitosan and fibroin. Surface hydrophilicity and water absorbency increased with blending fibroin. Above 20 $wt\%$ fibroin content, hydrogel film was formed. The surface and section of the film showed uniform microstructure on SEM photographs.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.49-52
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• 2001

The collagen growth effects in rats using B. mori fibroin sponge sheet, pure fibroin solution and chitosan sponge sheet were investigated. Histopathological inspection of the wound 12 days later showed the increse of a vascular ingrowth and the absence of inflammatory cells. Wound healing effects were accelerated in the order of Fibroin sponge sheet$\geq$Fibroin dressing solution〉Chitosan sponge sheet〉Control. The mean percentage of collagen production in rats after 12 days was 46.12$\pm$4.58% and 42.11$\pm$5.67% in fibroin sponge sheet and dressing solution-treated rats, respectively. These values were higher than the relative content in the chitosan sponge sheet and control groups, which was 23.87$\pm$3.24 and 2.42$\pm$0.32%, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.1
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• pp.59-64
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• 2000

The preparation of PVA/Chitosan/Fibroin (PCF)-blended sponge sheets and wound healing effects of these sheets in rats were investigated. We excised the skin off rat, including the dermis, approximately 2${\times}$2 cm in size. The wound was coveted with PCF-blended spongy sheets. The spongy sheets absorbed the exudate, and gained flexibility and softness. Histopathological inspection of the wound 12 days later showed the increase of a vascular ingrowth and the absence of inflammatory cells. Regeneration of the skin around the wound was faster than that of the control. We also tested wound healing effects of PVA, Chitosan and Fibroini alone of in various combinations. Wound healing was accelerated in the order of PVA/chitosan/Fibroin (PCF)-blended sponge>Chitosan/Fibroin (CF)-blended sponge$\geq$Fibroin (F) sponge>PVA/Chitosan-blended sponge (PC)>Chitosan (C) sponge.

• Polymer(Korea)
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• v.38 no.1
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• pp.54-61
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• 2014

Water-soluble sulfobetaine chitosan (SCs) was prepared for a blending film with Bombyx mori silk fibroin (SF) by reacting chitosan with 1,3-propanesultone. A series of SF/SCs blended films were successfully prepared by mixing aqueous solutions of B. mori SF and SCs. The SF/SCs blended films were examined through spectroscopic and thermal analysis to determine the morphological changes of SF in the SCs. The effects of the SF/SCs blend ratios on physical and mechanical properties were investigated to discover the feasibility of using these films as biomedical materials such as artificial skin and wound dressing. X-ray analysis showed good compatibility between the two biopolymers. The in vitro degradation behavior of the SF/SCs blended films was systematically investigated for up to 8 weeks in phosphate buffered saline solution at $37^{\circ}C$ and showed a mass loss of 46.4% after 8 weeks. All films showed no cytotoxicity by MC3T3-E1 assay. After 3 days of culture, the relative cell number on all the SF/SCs films was slightly lower than that of an optimized tissue culture plastic.

• Proceedings of the Korean Fiber Society Conference
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• 1998.10a
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• pp.45-48
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• 1998

카이토산(chitosan)은 항미생물성, 무독성, 인체적합성 및 양이온성 등의 특성을 지니며 항균, 방취, 보습, 생체적합성 및 생분해성 등의 다양한 기능을 나타내고 있는데, 이를 바탕으로 카이토산을 단독 또는 다른 천연섬유재료와 복합화시키는 연구가 행해지고 있다. 본 연구에서 선택한 카이토산과 복합체를 형성할 수 있는 성분인 견 피브로인(silk fibroin)은 17 종의 아미노산으로 이루어진 단백질로서 의류용 및 의료용 재료로서 이용되고 있는 고급섬유재료이다. (중략)

• Proceedings of the Korean Fiber Society Conference
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• 2003.10b
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• pp.267-268
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• 2003

실크 피브로인은 천연 고분자 물질 중 하나로 Bombyx mori와 Antheraea perni 등이 있으며 생체적 합성을 가지고 있어 효소고정화를 위한 매트릭스나 세포배양용 지지체와 같은 의료용 분야에서 활발히 연구되고 있다. 또한 키토산은 키틴이 50% 이상 탈아세틸화된 것으로 갑각류, 곤충류, 균류 등에 존재하며 창상피복재, 인조 피부 등의 의료 분야에서 광범위하게 사용되고 있다. 본 실험에서는 실크 피브로인에 단독으로는 전기방사가 되지 않는 키토산을 다양한 비율로 블렌드하여 키토산이 실크 피브로인의 전기방사된 섬유의 형태에 미치는 영향에 대하여 알아보았다. (중략)