Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Preparation of Sulfobetaine Chitosan, Silk Blended Films, and Their Properties

설포베타인 키토산의 실크 블렌드 필름의 제조 및 그들의 성질

  • Koo, Ja-Sung (Department of Nanobiomedical Science, Dankook University Graduate School) ;
  • Cha, Jae-Ryung (Department of Nanobiomedical Science, Dankook University Graduate School) ;
  • Oh, Se-Heang (Department of Nanobiomedical Science, Dankook University Graduate School) ;
  • Gong, Myoung-Seon (Department of Nanobiomedical Science, Dankook University Graduate School)
  • 구자성 (단국대학교 나노바이오의과학과) ;
  • 차재령 (단국대학교 나노바이오의과학과) ;
  • 오세행 (단국대학교 나노바이오의과학과) ;
  • 공명선 (단국대학교 나노바이오의과학과)
  • Received : 2013.08.20
  • Accepted : 2013.10.07
  • Published : 2014.01.25
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Abstract

Water-soluble sulfobetaine chitosan (SCs) was prepared for a blending film with Bombyx mori silk fibroin (SF) by reacting chitosan with 1,3-propanesultone. A series of SF/SCs blended films were successfully prepared by mixing aqueous solutions of B. mori SF and SCs. The SF/SCs blended films were examined through spectroscopic and thermal analysis to determine the morphological changes of SF in the SCs. The effects of the SF/SCs blend ratios on physical and mechanical properties were investigated to discover the feasibility of using these films as biomedical materials such as artificial skin and wound dressing. X-ray analysis showed good compatibility between the two biopolymers. The in vitro degradation behavior of the SF/SCs blended films was systematically investigated for up to 8 weeks in phosphate buffered saline solution at $37^{\circ}C$ and showed a mass loss of 46.4% after 8 weeks. All films showed no cytotoxicity by MC3T3-E1 assay. After 3 days of culture, the relative cell number on all the SF/SCs films was slightly lower than that of an optimized tissue culture plastic.

Bombyx mori silk fibroin(SF)과 블렌드 필름을 만들기 위하여 키토산에 1,3-propanesultone을 반응시켜 수용성 sulfobetaine chitosan(SCs)을 제조하였다. 여러 가지 비율의 SF/SCs 블렌드 필름을 B. mori SF와 SCs의 수용액을 혼합하여 제조하였다. 수용액으로부터 얻어진 SF/SCs 블렌드 필름의 구조와 형태 변화는 분광학적 및 열적 분석을 통해 규명하였다. SF와 SCs의 혼합 비율에 따른 인공 피부나 화상치료 목적의 비이오재료로서의 물리적 및 기계적 성질에 미치는 영향을 조사하였다. X-선 분석으로 두 생체고분자 사이에 좋은 친화성을 보여주고 있음을 알 수 있었으며 기계적 성질도 SCs의 함량이 증가하면 크게 증가하였다. $37^{\circ}C$에서 phosphate buffered saline solution 용액 중에서 in vitro 분해 실험을 8주 동안 시행한 결과 46.4%가 분해됨을 알 수 있었다. MC3T3-E1 세포에 의한 독성 실험 결과 무독성을 나타내 주었으며, 3일의 배양 후 SF/SCs 필름의 상대 세포 수는 최적화된 tissue culture plastic보다 약간 낮게 나타남을 알 수 있었다.

Keywords

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