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http://dx.doi.org/10.7317/pk.2014.38.1.54

Preparation of Sulfobetaine Chitosan, Silk Blended Films, and Their Properties  

Koo, Ja-Sung (Department of Nanobiomedical Science, Dankook University Graduate School)
Cha, Jae-Ryung (Department of Nanobiomedical Science, Dankook University Graduate School)
Oh, Se-Heang (Department of Nanobiomedical Science, Dankook University Graduate School)
Gong, Myoung-Seon (Department of Nanobiomedical Science, Dankook University Graduate School)
Publication Information
Polymer(Korea) / v.38, no.1, 2014 , pp. 54-61 More about this Journal
Abstract
Water-soluble sulfobetaine chitosan (SCs) was prepared for a blending film with Bombyx mori silk fibroin (SF) by reacting chitosan with 1,3-propanesultone. A series of SF/SCs blended films were successfully prepared by mixing aqueous solutions of B. mori SF and SCs. The SF/SCs blended films were examined through spectroscopic and thermal analysis to determine the morphological changes of SF in the SCs. The effects of the SF/SCs blend ratios on physical and mechanical properties were investigated to discover the feasibility of using these films as biomedical materials such as artificial skin and wound dressing. X-ray analysis showed good compatibility between the two biopolymers. The in vitro degradation behavior of the SF/SCs blended films was systematically investigated for up to 8 weeks in phosphate buffered saline solution at $37^{\circ}C$ and showed a mass loss of 46.4% after 8 weeks. All films showed no cytotoxicity by MC3T3-E1 assay. After 3 days of culture, the relative cell number on all the SF/SCs films was slightly lower than that of an optimized tissue culture plastic.
Keywords
sulfobetaine chitosan; silk fibroin; blended film; biocompatible polymer;
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