• Animal cells and systems
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• 제7권4호
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• pp.337-343
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• 2003

Methyl jasmonate (MeJA) is a signal molecule in the activation of defense responses in plants. In this study, we isolated 15 MeJA-inducible genes by subtractive hybridization. These genes encode two myrosinase-binding proteins, five lipase-like proteins, a polygalacturonase inhibitor, a putative chlorophyll-associated protein, a terpene synthase, a dehydroascorbate reductase, an ascorbate oxidase, a cysteine protease, an O-methyltransferase, and an epithiospecifier protein. Northern analysis showed that most of the Chinese cabbage genes are barely expressed in healthy leaves, but are strongly induced by MeJA treatment. We also examined whether these MeJA-inducible genes were activated by ethethon, BTH, and Pseudomonas syringae pv. tomato (Pst), a nonhost pathogen of Chinese cabbage. The results showed that none of the MeJA-inducible genes was strongly induced by ethephon or by BTH. The genes encoding lipase-like proteins and a myrosinase-binding protein were weakly induced by Pst. Other MeJA-inducible genes were not activated at all by the pathogen.

• Journal of Plant Biotechnology
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• 제7권4호
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• pp.219-223
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• 2005

We investigated chemical-wounding effect on Agrobacterium-mediated Chinese cabbage transformation via vacuum infiltration. Pre-germinated or germinating Chinese cabbage seeds were infiltrated with Agrobacterium tumefaciens LBA4404 cells carrying either GUS gene (pBI121) or hepatitis B virus surface antigen DNA (pBIHBsAg). Prior to agroinfiltration process, the seeds were soaked in sodium hydrosulfite (SHS) solution or just in sterile water as a control. Comparative transformation efficiency was determined by both of histochemistry and ELISA. We could demonstrate that SHS solution treatment especially to 1-day or 2-days old germinating seeds efficiently improved transformation process, and therefore, transient expression level. This strongly indicated that Agrobacterium infection could be facilitated indeed by SHS-causing wounds on Chinese cabbage seeds.

• 한국미생물·생명공학회지
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• 제17권4호
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• pp.403-406
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• 1989

To establish tile standard condition of uniformed Kimchi product, we introduced the concept of starter and studied the preparation of rapid-fermented Kimchi. Of the strains isolated from Kimchi, Kakdugi and infant's feces, M7 strain grew effectively on aseptic Chinese cabbage juice and on salted Chinese cabbage, the growth of M7 was decreased severely. Inoculated with M7 in salted Chinese cabbage, appropriate range of pH and lactic acid content were reached at 8-13 hrs and 12 hrs after addition of spices, respectively. The result of sensory evaluation was not significant at 5% level.

• Animal cells and systems
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• 제8권2호
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• pp.105-109
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• 2004

Aminoalcoholphosphotransferase catalyzes the synthesis of phosphatidylcholine and phosphatidylethanolamine from diacylglycerol plus a CDP-aminoalcohol such as CDP-choline or CDP-ethanolamine. Previously we suggested the presence of possible isoforms of this enzyme from Chinese cabbage roots and now report the cDNA cloning and expression analysis of AAPT3 encoding a third isoform of aminoalcoholphosphotransferase (AAPT3). AAPT3 contains an open reading frame of 1,176 bp coding for a protein of 392 amino acids. It shares 96 and 95% identity with Chinese cabbage AAPT1 and AAPT2, respectively, at the deduced amino acid level. The results from reverse transcriptase-polymerase chain reaction analysis indicate that expression of AAPT3 is up-regulated by low temperature as well as AAPT1 and AAPT2.

• 한국토양비료학회지
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• 제46권3호
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• pp.203-208
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• 2013

This study was conducted to investigate decomposition of livestock manure in soils cultivated with Chinese cabbage along an alitude gradient. The experiments were conducted in Kangreung (17 m above sea level), Bongpyeong (430 m above sea level), and Daekwanryeong (800 m above the sea level) in order to assess the decomposition rate and accumulations of livestock manures depending on different altitudes. During chinese cabbage cultivation, the decomposition ratios of organic matter derived form livestock manure expressed as % of the initial organic matter content were 42~48% for Kangreung, 26~29% for Bongpyeong and 10~14% for Daekwanryeong. Changes in air temperature with altitude might be a main factor affecting manure decomposition rates.

• 한국농공학회논문집
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• 제58권1호
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• pp.91-97
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• 2016

The objective of this study was to classify between radish and Chinese cabbage in autumn using hyperspectral images. The hyperspectral images were acquired by Compact Airborne Spectrographic Imager (CASI) with 1m spatial resolution and 48 bands covering the visible and near infrared portions of the solar spectrum from 370 to 1044 nm with a bandwidth of 14 nm. An object-based technique is used for classification of radish and Chinese cabbage. It was found that the optimum parameter values for image segmentation were scale 400, shape 0.1, color 0.9, compactness 0.5 and smoothness 0.5. As a result, the overall accuracy of classification was 90.7 % and the kappa coefficient was 0.71. The hyperspectral images can be used to classify other crops with higher accuracy than radish and Chines cabbage because of their similar characteristic and growth time.

• 한국식품과학회지
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• 제23권5호
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• pp.646-648
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• 1991

우리나라에서 풍부하게 생산될 수 있으면서 흔히 과잉생산되어 문제가 되는 배추를 이용한 단세포단백 생산에 관한 연구를 하였다. Saccharomyces cerevisiae와 Candida utilis 공히 배추즙액에 잘 번식하였으며 $30^{\circ}C$에서 20시간 이내에 번식이 완결되었다. 이들 효모의 최종 건조균체량은 동결시킨 후 해동된 배추를 이용하거나 동결 되지 않은 배추의 즙액을 이용하거나 차이가 없었는데 S. cerevisiae와 C. utilis는 각각 4.3g/l 및 5.1g/l의 건조균체를 생산하였다.

• Journal of Applied Biological Chemistry
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• 제48권4호
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• pp.229-230
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• 2005

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 하계 학술대회 논문집
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• pp.59-66
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• 2000

• 한국유기농업학회지
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• 제5권1호
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• pp.101-110
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• 1996

Inorder to minimize the daily intake amount of nitrate by chinese cabbage, the favorate vegetable for Korean, the influences of storage conditions at different temperature and light or dark treatments after post-harvest and effects of cooking temperature and infrared rays on changes of nitrate contents in edible parts of chinese cabbage were determined. The nitrate contents on midrib and leaf blade in chinese cabbage during post-harvest were decreased steeply in 2 days and decreased slowly again in 5 days. The temperature treatment to decrease the nitrate contents in midrib and leaf blade of chinese cabbage were effective as the following of $25^{\circ}C$> $15^{\circ}C$ > $-4^{\circ}C$ > $-10^{\circ}C$. The nitrate contents of midrib and leaf blade were decreased in the timecourse of post-harvest. It is more effective to store in $4^{\circ}C$ than in $15^{\circ}C$, and is more effective in transparent vinyl package than in black vinyl package, but the treatment of light is more effective than the treatment of temperature. The nitrate contents of midrib decreased rapidly by 17.9% in the treatment of 5 days at$ 15^{\circ}C$. By treatment of $80^{\circ}C$, $90^{\circ}C$, $100^{\circ}C$ water, the nitrate contents of midrib were increased slightly, but decreased in leaf blade. It reached 68.5%, 50.6%, 45.9% in the leaf blade respectively by treatment of 80%, 90%, 100% water at 1 min. By infrared rays treatment, the nitrate contents of midrib did not change in 3 min but increased rapidly after 6 min, and in the leaf blade it increased continually after 1 min.