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Cloning and Expression of a cDNA AAPT3 Encoding Aminoalcoholphosphotransferase Isoform from Chinese Cabbage  

Kim, Kwang-Soo (Department of Biological Sciences and Institute of Molecular Cell Biology, College of Sciences, Inha University)
Park, Jong-Ho (Department of Biological Sciences and Institute of Molecular Cell Biology, College of Sciences, Inha University)
Cho, Sung-Ho (Department of Biological Sciences and Institute of Molecular Cell Biology, College of Sciences, Inha University)
Publication Information
Animal cells and systems / v.8, no.2, 2004 , pp. 105-109 More about this Journal
Abstract
Aminoalcoholphosphotransferase catalyzes the synthesis of phosphatidylcholine and phosphatidylethanolamine from diacylglycerol plus a CDP-aminoalcohol such as CDP-choline or CDP-ethanolamine. Previously we suggested the presence of possible isoforms of this enzyme from Chinese cabbage roots and now report the cDNA cloning and expression analysis of AAPT3 encoding a third isoform of aminoalcoholphosphotransferase (AAPT3). AAPT3 contains an open reading frame of 1,176 bp coding for a protein of 392 amino acids. It shares 96 and 95% identity with Chinese cabbage AAPT1 and AAPT2, respectively, at the deduced amino acid level. The results from reverse transcriptase-polymerase chain reaction analysis indicate that expression of AAPT3 is up-regulated by low temperature as well as AAPT1 and AAPT2.
Keywords
Aminoalcoholphosphotransferase; Chinese cabbage; Low temperature; phosphatidylcholine; phosphatidylethanolamine;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Kyte J and Doolittle RF (1982) A simple method displaying the hydropathic character of a protein. J Mol Biol 57: 105-132   DOI
2 Min KM, Bae YG, Lee JS, Choi YH, Cha YR, and Cho SH (1997) Cloning of an aminoalcoholphosphotransferase cDNA from Chinese cabbage roots. J Plant Biol 40: 234-239   과학기술학회마을   DOI
3 Moore TS (1982) Phospholipid biosynthesis. Annu Rev Plant Physiol 33: 235-259   DOI
4 Macher BA and Mudd JB (1974) Biosynthesis of phosphatidylethanolamine by enzyme preparations from plant tissues. Plant Physiol 53: 171-175   DOI   ScienceOn
5 Mancini A, Del Rosso F, Roberti R, Orvietani P, Coletti L, and Binaglia L (1999) Purification of ethanolaminephosphotransferase from bovine liver microsomes. Biochim Biophys Acta 1437: 80-92   DOI   ScienceOn
6 McMaster CR and Bell RM (1997) CDP-choline: 1,2-diacylglycerol cholinephosphotransferase. Biochim Biophys Acta 1348: 100-110   DOI   ScienceOn
7 Lee JK, Choi YH, Bae YG, Lee WS, and Cho SH (2001) Cloning and expression of a cDNA encoding aminoalcoholphosphotransferase from Pimpinella brachycarpa. J Plant Biol 44: 27-32   과학기술학회마을   DOI
8 Lord JM (1975) Evidence that phosphatidylcholine and phosphatidylethanolamine are synthesized by a single enzyme present in the endoplasmic reticulum of castorbean endosperm. Biochem J 151: 451-453   DOI
9 Kinney AJ, Clarkson DT, and Loughman BC (1987) The regulation of phosphatidylcholine biosynthesis in rye roots: stimulation of the nucleotide pathway by low temperature. Biochem J 242: 755-759   DOI
10 Kinney AJ (1993) Phospholipid head groups. In: Moore TS (ed), Lipid Metabolism in Plants, CRC, Boca Raton, pp 259-284
11 Horvath I, Vigh L, and Farkas T (1981) The manipulation of polar head group composition of phospholipids in the wheat Miranovskaja 808 affects frost tolerance. Planta 151: 103-108   DOI   ScienceOn
12 Frohman MA, Dush MK, and Martin GR (1998) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998-9002   DOI
13 Inatsugi R, Nakamura M, and Nishida I (2002) Phosphatidylcholine biosynthesis at low temperature: differential expression of CTP: phosphorylcholine cytidylyltransferase isogenes in Arabidopsis thaliana. Plant Cell Physiol 43: 1342-1350   DOI   ScienceOn
14 Justin AM, Demandre C, Tremolieres A, and Mazliak P (1985) No discrimination by choline- and ethanolaminephosphotransferase from potato tuber microsomes in molecular species of endogenous diacylglycerols. Biochim Biophys Acta 836: 1-7   DOI
15 Kennedy EP and Weiss SB (1956) The function of cytidine coenzymes in the biosynthesis of phospholipids. J Biol Chem 222: 193-214
16 Goode JH and Dewey RE (1999) Characterization of aminoalcoholphosphotransferases from Arabidopsis thaliana and soybean. Plant Physiol Biochem 37: 445-457   DOI   ScienceOn
17 Harwood JL (1979) The synthesis of acyl lipids in plant tissues. Prog Lipid Res 18: 55-86   DOI   ScienceOn
18 Hjelmstad RH and Bell RM (1991) sn-1,2-diacylglycerol choline-and ethanolaminephosphotransferase in Saccharomyces cerevisiae: mixed micellar analysis of the CPT1 and EPT1 gene product. J Biol Chem 266: 4357-4365
19 Harwood JL (1998) Environmental effects on plant lipid biochemistry. In: Harwood JL (ed), Plant Lipid Biosynthesis, Cambridge University Press, Cambridge, pp 305-347
20 Harwood JL, Jones AL, Perry HJ, Rutter AJ, Smith KL, and Williams M (1994) Changes in plant lipids during temperature adaptation. In: Cossins AR(ed), Temperature Adaptation of Biological Membranes, Portland Press, London, pp 107-118
21 Cho SH and Cheesbrough TM (1990) Warm growth temperatures decrease soybean cholinephosphotransferase activity. Plant Physiol 93: 72-76   DOI   ScienceOn
22 Choi YH, Lee JK, Lee C-H, and Cho SH (2000) cDNA Cloning and expression of an aminoalcoholphosphotransferase isoform in Chinese cabbage. Plant Cell Physiol 41: 1080-1084   DOI   ScienceOn
23 Weiss SB, Smith SW, and Kennedy EP (1958) The enzymatic formation of lecithin from cytidine diphosphate choline and D1,2-diglyceride. J Biol Chem 231: 53-64
24 Chou PY and Fasman GD (1978) Empirical predictions of protein conformation. Annu Rev Biochem 47: 251-276   DOI   ScienceOn
25 Dewey RE, Wilson RF, Novitzky WP, and Goode JH (1994) The AAPT1 gene of a soybean complements: a cholinephosphotransferase-deficient mutant of a yeast. Plant Cell 6: 1495-1507   DOI   ScienceOn
26 Belyavsky AT, Vinogradova T, and Rajewsky K (1989) PCR-based cDNA libraries at the level of a few cells. Nucl Acids Res 17: 2919-2932   DOI
27 Cheesbrough TM (1989) Changes in the enzymes for fatty acid synthesis and desaturation during acclimation of developing soybean seeds to altered growth temperature. Plant Physiol 90: 760-764   DOI   ScienceOn
28 Qi Q, Huang YF, Cutler AJ, Abrams SR, and Taylor DC (2003) Molecular and biochemical characterization of an aminoalcoholphosphotransferase (AAPT1) from Brassica napus: effects of low temperature and abscisic acid treatments on AAPT expression in Arabidopsis plants and effects of over-expression of BnAAPT1 in transgenic Arabidopsis. Planta 217: 547-558   DOI
29 Sparace SA, Wagner LK, and Moore TS (1981) Phosphatidylethanolamine synthesis in castor bean endosperm. Plant Physiol 67: 922-925   DOI   ScienceOn
30 Vance DE (1989) CTP: cholinephosphate cytidylyltransferase. In: Vance DE (ed), Phosphatidylcholine Metabolism, CRC, Boca Raton, pp 34-45
31 Williams JG and McMaster CR (1998) Scanning alanine mutagenesis of the CDP-alcohol phosphotransferase motif of Saccharomyces cerevisiae cholinephosphotransferase. J Biol Chem 273: 13482-13487   DOI   ScienceOn
32 Williams M and Harwood JL (1997) Effects of carbon dioxide concentration and temperature on lipid synthesis by young wheat leaves. Phytochemistry 45: 243-250   DOI   ScienceOn