• 대한수의학회지
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• 제38권4호
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• pp.784-792
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• 1998

This study was conducted to select resistant chicken-line between Brown and White layer lines against Salmonella gallinarum infection. The Brown and White layer chickens allocated into different age groups were inoculated with S gallinarum (WJO-126) either orally($1{\times}10^7cfu$) or intramusculaly($5{\times}10^6cfu$) and clinical observations were made for 2 weeks. All dead birds were necropsied and culture was made to recover the inoculated organinsm from liver, spleen, brain, bone marrow and cecal contents. Serum was isolated from all live birds after 2 weeks experiment and these birds were also necropsied and cultured to reisolate S gallinarum. The brown layers showed very high mortality to S gallinarum infection regardless of their ages and routes of inoculation, while white layers did not shown any mortality by the direct effects of S gallinarum. The mortality rate of 2 week old brown layers, in particular, were 82.6% (19/23) in peroral group and 86.9% in intramuscularly inoculated group, while those of white layer groups were only 0.0% in both groups. S gallinarum could be reisolated from all dead birds, especially, from liver and spleen. This result was inferred that the organism is highly invasive on the chicken. The intramuscularly challenged birds showed more seropositive-reactors(86.9%) than orally inoculated groups(61.9%). The overall results of present study suggested that white layers are much more resistant than brown layers against the experimental infection of S gallinarum and shown experimentally that resistance to S gallinarum is a characteristic of the White-line layers.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1229-1236
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• 2012

Our previous studies showed that kisspeptin-10 (Kp-10) injected in vivo can markedly increase lipid anabolism in liver of quails. In order to investigate the direct effect of Kp-10 on lipid metabolism of hepatocytes in birds, cells were separated from embryos livers and cultured in vitro with 0, 100 and 1,000 nM Kp-10, respectively. The results showed that after 24 h treatment, cells viability was not affected by 100 nM Kp-10, but showed a mild decrease with 1,000 nM Kp-10 compared to the control cells. Based on the results of the cell viability, 100 nM dosage of Kp-10 was selected for the further study and analysis. Compared with control cells, total cholesterol (Tch) contents in 100 nM treated cells were increased by 51.23%, but did not reach statistical significance, while the level of triglyceride (TG), high density of lipoprotein-cholesterol (HDL-C) and low density of lipoprotein-cholesterol (LDL-C) were significantly increased. Real-time PCR results showed that ApoVLDL-II mRNA expression had a tendency to increase, genes including sterol regulatory element-binding protein-1 (SREBP-1), acetyl coenzyme A carboxylase ${\alpha}$ ($ACC{\alpha}$), carnitine palmitoyltransferase 1 (CPT1), 3-hydroxyl-3-methylglutaryl-coenzyme A reductases (HMGCR) and stearyl coenzyme A dehydrogenase-1 (SCD1) mRNA in hepatocytes were significantly down-regulated by 100 nM Kp-10. However, contrary to its gene expression, SREBP-1 protein expression was significantly up-regulated by 100 nM Kp-10. Some of the significant correlations in mRNA expression were found between genes encoding hepatic factors or enzymes involved in lipid metabolism in liver of birds. These results indicate that Kp-10 stimulates lipid synthesis directly in primary cultured hepatocytes of chickens.

• 한국식품과학회지
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• 제10권3호
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• pp.313-319
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• 1978

무지방사료(無脂肪飼料) 및 여기에 17:0, 18:0 혹은 18:2을 첨가(添加)한 사료(飼料)를 병아리에 급여(給與)해서 간장(肝臟) 및 혈장(血漿)의 각지질분획(各脂質分劃)의 지방산조성(脂肪酸組成)에 미치는 급여지방산(給與脂肪酸)의 영향(影響)을 조사(調査)하였다. 그 결과(結果) 간장중(肝臟中)의 총지질함량(總脂質含量) 및 각지질분획(各脂質分劃)의 함량(含量)은 급여지방산(給與脂肪酸)에 의해서 영향(影響)받지 않았다. 그러나 17:0을 급여(給與)하면 간장(肝臟) 및 혈장(血漿)의 각지질분획(各脂質分劃)에 17:0 및 17:1 출현(出現)하고, 혈장(血漿)트리그리세라이드에서는 17:0이 또한 혈장(血漿)콜레스테롤에스텔에서는 17:1이 다른 분획(分劃)보다도 유의(有意)하게 높았다. 더욱이 간장(肝臟)에서는 각분획(各分劃)사이에 양지방산(兩脂肪酸)의 분포치(分布値)에 큰 차이(差異)가 없었다. 18:2의 급여(給與)도 17:0을 급여(給與)한때와 같이 간장(肝臟) 및 혈장(血漿)의 각지질분획(各脂質分劃)에 18:2이 출현(出現)하고, 특(特)히 간장인지질분획(肝臟燐脂質分劃)에서 18:2의 분포치(分布値)는 다른분획(分劃)보다도 유의(有意)하게 높았다. 그러나 18:0을 급여(給與)해도 이것이 간장(肝臟) 및 혈장중(血漿中)에 특별적(特別的)으로 증가(增加)하지는 않았다. 또한 17:0 및 18:2과 같은 외인성지방산(外因性脂肪酸)의 급여(給與)에 의하여 내인적(內因的)으로 합성(合成)되는 16:1 및 18:1의 분포치(分布値)는 간장(肝臟)에서 감소(減少)하는 경향(傾向)을 나타내었다.

• 한국식품과학회지
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• 제42권5호
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• pp.605-612
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• 2010

황기열수추출물(WEH)과 홍국 70% 에탄올추출물(EEB) 및 Monascus pilosus 액침배양액(LBK)을 동결건조한 등량 혼합물을 일반 닭고기 베이스소스에 0.225% 첨가한 황기홍국소스(HBS)를 처리한 계육의 식이가 고지방-고콜레스테롤 병행식이 흰쥐의 ROS 생성 및 소거계 효소류의 활성에 미치는 영향을 조사하였다. 실험군은 정상군(NC), 고지방-고콜레스테롤 대조군(HFC), 고지방-고콜레스테롤 식이에 베이스소스를 처리한 계육을 15% 첨가한 식이군(HFC-BS) 및 고지방-고콜레스테롤 식이에 황기홍국소스를 처리한 계육을 15% 첨가한 식이군(HFC-HBS)으로 구분하여(5마리/군) 5주간 사육하였다. 체내 활성산소를 생성하는 xanthin oxidoreductase O type 활성은 HFC군에 비하여는 31.84%가 HFCBS군에 비하여는 24.57%가 각각 감소되었다. Superoxide dismutase 및 glutathione S-transferase 활성은 HFC-HBS군이 HFC군 및 HFC-BS군에 비하여 각각 25.17-64.50% 및 19.29-62.89%가 증가 하였다. 활성산소에 소거에 관여하는 glutathione의 함량은 HFCHBS군이 HFC군 및 HFC-BS군에 비하여 각각 53.30% 및 25.11%가 증가하였으며, 간조직의 지질과산화물의 함량은 각각 20.29% 및 24.19%가 감소되었다. Glutathione peroxidase 및 catalase 활성은 각각 41.29-41.87% 및 22.91-23.44%가 증가하였다. 이상의 결과 황기홍국소스를 처리한 계육은 고지방-고콜레스테롤 병행식이 하에서도 활성산소 생성계 효소 활성을 감소함과 동시에 소거계 활성을 높힘으로서 간조직 손상을 예방 및 치유하는 것으로 사료된다.

• BMB Reports
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• 제42권9호
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• pp.568-573
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• 2009

Fatty acid oxidation (FAO) defects cause abnormal lipid accumulation in various tissues, which provides an opportunity to uncover novel genes that are involved in lipid metabolism. During a gene expression study in the riboflavin deficient induced FAO disorder in the chicken, we discovered the dramatic increase in mRNA levels of an uncharacterized gene, ANKRD9. No functions have been ascribed to ANKRD9 and its orthologs, although their sequences are well conserved among vertebrates. To provide insight into the function of ANKRD9, the expression of ANKRD9 mRNA in lipidperturbed paradigms was examined. The hepatic mRNA level of ANKRD9 was repressed by thyroid hormone ($T_3$) and fasting, elevated by re-feeding upon fasting. However, ANKRD9 mRNA level is reduced in response to apoptosis. Transient transfection assay with green fluorescent protein tagged- ANKRD9 showed that this protein is localized within the cytoplasm. These findings point to the possibility that ANKRD9 is involved in intracellular lipid accumulation.

• 대한수의학회지
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• 제48권4호
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• pp.469-472
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• 2008

Necropsy was performed on a total of twenty three either white leghorn or ogol chickens which were more than 150 weeks of age. Among twenty three chickens examined, fifteen chickens were laying and the rest eight chickens were non-laying. On necropsy, neoplastic mass in the five chickens among non-laying chickens was found. These neoplastic masses were present mostly in the ovaries and one case in the liver and characterized by multifocal to coalescing 1 to 5 mm tan firm nodular formation. On histopathology, ovarian adenocarcinoma with widespread abdominal seeding and hepatic metastasis was diagnosed in the three chickens. Oviductal leiomyoma was also found in two chickens that had a focal well-demarcated nodules in the oviduct. Taken together, the number with ovarian adenocarcinoma among non-laying chickens over 150 week old was 37.5%. As most animal species do rarely develop ovarian tumors, the high rate of spontaneous ovarian adenocarcinoma in non-laying hen suggest that the hen is a proper model for human ovarian cancer study.

• 한국동물위생학회지
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• 제32권2호
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• pp.155-163
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• 2009

Fowl typhoid (FT) is a septicemic disease caused by Salmonella Gallinarum. The purpose of this study was to investigate the antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of S. Gallinarum isolated from chicken. During 1999 to 2004, there was isolated a total of 100 strains in liver and spleen. The biochemical characteristics of S. Gallinarum isolates was nonmotile, no production of H$_2$S, glucose gas, non-fermented rhamnose, indole-negative, fermentation of dulcitol, mannitol, maltose, and ornithine decarboxylase. At antimicrobial susceptibility, all of isolates were susceptible to amoxicillin/clavulanic acid, amikacin, neomycin, kanamycin, norfloxacin and enrofloxacin. One hundred isolates were divided into 54 resistant patterns and 37 strains was 6-multi drug resistance. PFGE of Xba I restriction fragments of S. Gallinarum isolates was 20 patterns.

• 동아시아식생활학회지
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• 제1권3호
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• pp.259-276
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• 1991

The review of side dishes, oereal dishes and seasonings of the banquet on the 24th year of King Kojong in Chosun Dynasty were summarized as follows: 1. Ten kinds of soups(=Tang), that is Yyolguja Tang$.$Guja Tang$.$Keumjung Tang$.$Whanja Tang$.$Jap Tang$.$Gol Tang$.$Seunggiwa Tang$.$Jeopo Tang$.$Haesam Tang$.$Yang Tang were served. The most frequently served soup among them was Yyolguja Tang. 2. Various kinds of dried meat/fish were piled up most highly among dishes. Sliced beef and minced beef among dried meats, white cud$.$yellow cud$.$flatfish$.$skate$.$shark$.$octopus$.$dried abalone$.$cuttle fish$.$dried scallop were piled up together. 3. Sliced of boiled beef/pork and gelatined knuckles were assorted. Boiled meat which was made of beef, pork, lamb, liver, stomach, brisket and plat flank of caw was chiefly used for boiled beef, hams for boiled pork, mediumsized lamb for boiled lamb. And the legs and stomach of caw, old chicken, mullet were used for gelatined knuckle. 4. Three kinds of pan-frying dishes that was stomach and liver of caw, and mullet were usually piled up, If one kind in one dish, sea cucumber and mullet were used respectively. 5. Boiled abalone was dried abalone with soaked, sliced and boiled down in soysauce. 6. Roasted dish was usually made of assorted food stuffs. Sometimes chicken an pheasant was used for roasted dish. 7. Steamed dish was like this : assorted beef, fresh abalone, sea cucumber, mullet, young pork, chicken, dried abalone. 8. Raw dish was made of intestine of caw, fresh abalone and mullet. 9. Poached egg was serving decorated with red pepper, pine-nut and green onion. 10. Seasoned green-been-jelly was served only one time. 11. Radish kimchi was served only one time. 12. Chief foods were nuddle and stuffed bun. 13. Served seasings were honey, mustard, soysauce with vinegar, salt. Review from banquet menu, 34 kinds of side dishes were served : 10 kinds of soup, dried fish/meat, boiled beef, boiled pork, gelatined knuckle, 5 kinds of panfrying, boiled-down-abalone in soysauce, 3 kinds of roast, 5 kinds of steamed, 3 kinds of raw dishes, pouched egg, green-bean-jelly, kimchi. 4 kinds of chief foods were served : nuddle, dry nuddle, 2 kinds of dumping.

• 한국축산식품학회지
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• 제34권2호
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• pp.172-177
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• 2014

Two experiments were conducted to investigate the dietary effects of conventional or lutein fortified chlorella on lutein absorptions, the tissue distributions and the changes in lutein content of eggs in laying hens. In Exp 1, a total of one hundred and fifty, 70 wk-old Hy-Line brown layers were divided into three groups with five replicates and fed with each experiment diet (control diet, diet with 1% conventional chlorella or lutein fortified chlorella) for 2 wk, respectively. The egg production in groups fed diets containing both chlorella powders were higher than that of the control group (p<0.01). With chlorella supplementations, the yolk color significantly increased, although there were no significant differences in the eggshell qualities. The lutein contents of serum, liver and growing oocytes were greatly increased by feeding conventional or lutein fortified chlorella (p<0.01). In Exp. 2, a total of ninety 60 wk-old Hy-Line brown layers were assigned into three groups with three replicates per group (10 birds per replicate). The birds were fed with one of three experimental diets (0, 0.1 or 0.2% lutein fortified chlorella) for 2 wk, respectively. The egg production was not affected by dietary treatments. The egg weight in the group fed with diet containing 0.2% of lutein fortified chlorella was higher than that of the control (p<0.05). As the dietary chlorella levels increased, the daily egg mass linearly increased, although not significantly. The yolk colors in groups fed diets containing lutein fortified chlorella were dramatically increased as compared to the control (p<0.001). The lutein in chicken eggs significantly increased when fed with 0.2% of lutein fortified chlorella (p<0.01). These results suggested that the dietary lutein derived from chlorella was readily absorbed into the serum and absorbed by the liver with growing oocyte for commercial laying hens. Particularly, the lutein fortified chlorella was a valuable natural source for the production of lutein enriched chicken eggs.

• 한국가금학회지
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• 제21권4호
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• pp.227-237
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• 1994

This study was conducted to investigate the interaction of vitamins A and E on their transfer from diet to chicken eggs and the effect of vitamin A flood-dosing on its concentration in eggs and livers. In Experiment I, forty-two 45-wk-old brown layers (Bobeock) were divided into seven groups and fed one of seven diets: control, three vitamin A supplemented diets(8, OOO, 16, 000, and 64, 000 IU /kg diet) or three vitamin E supplemented diets (50, 100, and 200 IU/kg diet). In Experiment II, a total of thirty-two 35-wk-old white layers (Hy-ine) were divided into four groups and fed one of four diets :control, vitamin A 20, 00O+vitamin E 200 TU /kg, vitamin A 50, O00+vitamin E 200 IU /kg or vitamin A 100, OO0+vitamin E 200 lU/kg supplemented diets. In Experiment III, a total of fifty-six 35-wk-old white layers (Hy-line) was divided into four groups and fed one of four diets: control or three vitamin A supplemented diets (80, 000, 120, 000 and 160, 000 lU/kg diet). In Experiment I, vitamin E levels of egg yolk in hens fed the vitamin A supplemented diets decreased as dietary vitamin A level increased (P