Browse > Article

Antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of Salmonella Gallinarum isolated from chicken  

Bae, Jong-Chul (Gyeongbuk Veterinary Service Laboratory)
Kim, Seong-Guk (Gyeongbuk Veterinary Service Laboratory)
Kim, Young-Hoan (Gyeongbuk Veterinary Service Laboratory)
Jo, Min-Hee (Gyeongbuk Veterinary Service Laboratory)
Lee, Young-Ju (Gyeongbuk Veterinary Service Laboratory)
Park, Cheong-Kyu (College of Veterinary medicine, Kyungpook national University)
Publication Information
Korean Journal of Veterinary Service / v.32, no.2, 2009 , pp. 155-163 More about this Journal
Abstract
Fowl typhoid (FT) is a septicemic disease caused by Salmonella Gallinarum. The purpose of this study was to investigate the antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of S. Gallinarum isolated from chicken. During 1999 to 2004, there was isolated a total of 100 strains in liver and spleen. The biochemical characteristics of S. Gallinarum isolates was nonmotile, no production of H$_2$S, glucose gas, non-fermented rhamnose, indole-negative, fermentation of dulcitol, mannitol, maltose, and ornithine decarboxylase. At antimicrobial susceptibility, all of isolates were susceptible to amoxicillin/clavulanic acid, amikacin, neomycin, kanamycin, norfloxacin and enrofloxacin. One hundred isolates were divided into 54 resistant patterns and 37 strains was 6-multi drug resistance. PFGE of Xba I restriction fragments of S. Gallinarum isolates was 20 patterns.
Keywords
Salmonella Gallinarum; Antimicrobial susceptibility; PFGE;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 김성극, 김영환, 영현정, 장성준, 조굉현, 이양수. 2006. 육계에서 분리한 Salmonella gallinarum의 약제내성 및 PFGE 양상. 한국가축위생학회지 29: 297-308
2 박노찬, 도재철, 조광현, 장성준, 권한일, 박덕상. 1995 닭티푸스균의 발생상황파 Salmonella gallinarum 의 항균제 감수성. 한국가축위생학회지 18: 113-123
3 오강희, 김삭환, 이경현, 하종수, 박승춘, 정규식, 정종식, 이근우, 송재찬. 2002. 닭에서 분리한 Salmonella gallinanum의 병윈성 및 plasmid profile. 한국임상수의학회지 19(2): 159-164
4 우용구, 검봉환. 1998. 가금티푸스균의 인공감염에 대한 백색 및 갈색 산란계 계통간의 내병성 비교. 대한수의학회지 38: 784-792
5 이동석, 한태욱. 2000 국내에서 분리한 Salmonella gallinarum의 병원성, 항생세 간수성 및 plasmid profile. 한국수의공중보건학회지 24(1): 49-57
6 Edward PR, Bruner DW, Dowell ER. 1948. Salmonella infections of fowls. Cornell Vet 38: 257-262   PUBMED
7 Garaizar J, Lopez-Molina N, Laconcha I, Lau Baggesen D, Rementcria A, Vivanco A, Audicana A, Perales I 2000. Suitability of PCR fingerprinting, infrequcntrestriction-site PCR and pulsed-field gel electro phoresis, combincd with computerized ge) analysis, in library typing of Salmonclla enterica serovar Enteritidis. Appl Eenviron Microbiol 66(12): 5273-5281   DOI   ScienceOn
8 Klein E. 1889. Uber eine epidenusche Krankheit der Huhne, verursacht durch einer Bacillus-Bacillus gallinarum. Zentralbl Baktcriol. Paraxitenkd Abt I Orig 5: 689-693
9 Saif YM, Bames HJ, Fadly AM, Glisson JR, McDougald LR, Swayne DE. 2003. Diseases of poultry. 11 ed. Blackwell Publishing Co, Iowa: 567-579
10 Ewing WH. 1986. Edwards and Ewing's identification of Enterobacteriaceae. 4ed. Elsevier, Amsterdam: 181-318
11 Smith HW, Tucker JF. 1980. The virulence of Salmonella strain for chickens : Their excretion by infcctcd chickens. J Hyg 84: 479-488   DOI   ScienceOn
12 Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Pcrsing DH, Swaminathan B. 1995. Interpreting chromosomal DNA restriction patterns produccd by pulsed-field gel electrophoresis : criteria for bactcrial strain typing. J Clin MicrobioI 33(9): 2233-2239
13 McPeek FD, Coyle-Moπis JF. Gemmill RM. 1986. Separation of large DNA molecules by modified pulsed field gradicnt gcl eleclrophoresis. Anal Biochem 156(2): 274-285   DOI   ScienceOn
14 Seo YS, Lee HS. Shin KE, Kim SJ, Jung R, Hahn TW. 2006. Pulsed-field gel electrophoresis genotyping of Salmonclla gallinaruln and comparisoll with random amplified polymorphic DNA. Vet Microbiol 115(4): 349-357   DOI   ScienceOn
15 Calnek BW, Barnes HJ, Bεard CW, McDougald LR, Saif YM. 1997. Diseases of poultry, 10 ed. Mosby-Wolfe Co, London: 82-96.
16 Lee YJ, Kim KS, Kwon YK, Tak RB. 2003. Biochemical characteristics and antimicrobials susceptibility of Salmonella gallinarum isolated in Korea. J Vet Sci 4(2): 161-166   PUBMED
17 Lee YJ, Kim BH, Kim KS. 2004. analysis of Salmonella gallinarum isolates by randomly amplified polymorphic DNA. Kor J Vet Publ Hlth 28(1): 1-5
18 Gemer-Smidt P, Gravεs LM, Hunter S, Swaminathan B. 1998 Computεrized analysis of restriction fragment length polymorphism patterns: Comparative evaluation of two commercial software packages. J Clin Microbiol 36(5): 1318-1323   PUBMED
19 Olson MV. 1989. Separation of large DNA molecules by pulsed-field gcl electrophorcsis. A review of the basic phenomenology. J Chromator 470(2): 377-383   DOI   ScienceOn
20 Allardet-servent A, Bourg G, Ramuz M, Pages M, Bellis M, Roizes G. 1988. DNA polYl1lorphism in slrains of the genus brucella. J Bacteriol 170(10): 4603-4607   DOI   PUBMED
21 Clinical and Laboratory Slandards Instilule. 2002. Per-formance slandards for antimicrobial disk suscepti-bility tests for bacteria isolated from animals. 2nd. NCCLS document M31-A3, Wayne, Pcnnsylvania
22 CDC, Pulsenet. 2004. Slandardized laboralory protoco! for molecular subtyping of Escherchia coli 0157:H7, nontyphoidal Salmonella sεrotypes, and Shigella sonnei by PFGE. http://www.cdc.gov/pulsenet/protocols/
23 박경윤. 1999. 국내 가금류에서 의 살모넨라감염중 발생상황과 살모넬라 분리주의 특성조사. 서울대학교 박사학위논문
24 Christensen JP, Olsen JE, Hansen HC, Bisgaard M. 1992. Characterization of Salmonella enferica serovar gallinarum biovars gallinarum and pullorum by plasmid profiling and biochemical analysis. Avian Pathol 21: 461-470   DOI   PUBMED
25 Hudson CR. Quist C, Lee MD, Kεyes K, Dodson SV, Morales C, Sanchez S, White DG' Maurer JJ. 2000. Genetic relatεdness of Salmonella isolates from nondomestic birds in Southeastcm United States. J Clin Microbiol 38(5): 1860-1865   PUBMED
26 김기석, 이희수, 모인필. 1995. 국내 닭에서의 가금티푸스 발생. 농촌진흥청 농업과학논문집 37(1): 544-549
27 이회수, 검순재, 김기석, 모인필, 우용구, 권용국, 김태종. 1997. Salmonella gallinarum 분리주로부터 추출한 세포외막 단백질의 닭에 대한 면역원성. 대한수의학회지 37(3): 501-510