• 약학회지
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• 제50권5호
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• pp.301-307
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• 2006

A simple and sensitive high-performance liquid chromatographic method for quantitation of hydrochlorothiazide in human plasma was developed and bioavailability parameters of hydrochlorothiazide were assessed in Korean healthy male volunteers. Caffeine was used as an internal standard. Hydrochlorothiazide and internal standard in plasma sample were extracted using tert-butylmethylether (TBME). A centrifuged upper layer was then evaporated and reconstituted with mobile phase of acetonitrile-25 mM phosphate buffer (20/80, pH 2.5). The reconstituted samples were injected into a Luna C18 column $(250{\times}4.6\;mm,\;5{\mu}m)$ at a flow-rate of 1.0 ml/min. The wavelength was set at 230 nm and no endogenous substances were found to interfere, A linear relationship for hydrochlorothiazide was found in the range of $10{\sim}300\;ng/ml$. The lower limit of quantitation (LLOQ) was 10 ng/ml with acceptable precision and accuracy. Assayed in plasma, the intra- and inter-day validation for all coefficients of variation (R.S.D.%) were found less than 15%. Main pharmacokinetic parameters of 50mg of hydrochlorothiazide were revealed as follows: $AUC_t\;1761{\pm}509.0\;ng{\cdot} hr\;ml,\;C_{max}\;296.5{\pm}95.5\;ng/ml,\;T_{max}\;1.94{\pm}0.85hr,\;K_{el}\;0.12{\pm}0.04\;hr^{-1}\;and T_{12}\;6.81{\pm}2.92\;hr.\;C_{max}\;and\;T_{max}$ were in accordance with the values $(270{\sim}350\;ng/ml\;and\;1.9{\sim}2.7\;hr)$ of Caucasian.

• Industrial Engineering and Management Systems
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• 제12권4호
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• pp.380-388
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• 2013

Large variation in electrocardiogram (ECG) waveforms continues to present challenges in defining R-wave locations in ECG signals. This research presents a procedure to extract the R-wave locations by forward-backward (FB) algorithm and classify the arrhythmic beat conditions by using RR intervals. The FB algorithm shows forward and backward searching rules from QRS onset and eliminates lower-amplitude signals near the baseline using a statistical process control concept. The proposed algorithm was trained the optimal parameters by using MIT-BIH arrhythmia database (MITDB), and it was verified by actual Holter ECG signals from a local hospital. The signals are classified into normal (N) and three arrhythmia beat types including premature ventricular contraction (PVC), ventricular flutter/fibrillation (VF), and second-degree heart block (BII) beat. This work produces 98.54% accuracy in the detection of R-wave location; 98.68% for N beats; 91.17% for PVC beats; and 87.2% for VF beats in the collected Holter ECG signals, and the results are better than what are reported in literature.

• International Journal of Internet, Broadcasting and Communication
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• 제12권3호
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• pp.125-130
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• 2020

At the end of 1997, the volatility of the exchange rate intensified as the nation's exchange rate system was converted into a free-floating exchange rate system. As a result, managing the exchange rate is becoming a very important task, and the need for forecasting the exchange rate is growing. The exchange rate prediction model using the existing exchange rate prediction method, statistical technique, cannot find a nonlinear pattern of the time series variable, and it is difficult to analyze the time series with the variability cluster phenomenon. And as the number of variables to be analyzed increases, the number of parameters to be estimated increases, and it is not easy to interpret the meaning of the estimated coefficients. Accordingly, the exchange rate prediction model using artificial neural network, rather than statistical technique, is presented. Using DNN, which is the basis of deep learning among artificial neural networks, and LSTM, a recurrent neural network model, the number of hidden layers, neurons, and activation function changes of each model found the optimal exchange rate prediction model. The study found that although there were model differences, LSTM models performed better than DNN models and performed best when the activation function was Tanh.

• 보건행정학회지
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• 제12권2호
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• pp.1-22
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• 2002

Since the early 1980s scholars have applied latent structure and other type of finite mixture models from various academic fields. Although the merits of finite mixture model are well documented, the attempt to apply the mixture model to medical service has been relatively rare. The researchers aim to try to fill this gap by introducing finite mixture model and segmenting inpatients DB from one general hospital. In section 2 finite mixture models are compared with clustering, chi-square analysis, and discriminant analysis based on Wedel and Kamakura(2000)'s segmentation methodology schemata. The mixture model shows the optimal segments number and fuzzy classification for each observation by EM(expectation-maximization algorism). The finite mixture model is to unfix the sample, to Identify the groups, and to estimate the parameters of the density function underlying the observed data within each group. In section 3 and 4 we illustrate results of segmenting 4510 patients data including menial and ratio scales. And then, we show AHP can be identify the attractiveness of each segment, in which the decision maker can select the best target segment.

• 한국자기학회지
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• 제13권1호
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• pp.21-28
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• 2003

BaF $e_{12-2x}$ $Co_{x}$ $Ti_{x}$ $O_{19}$ (0$\leq$x$\leq$1.0) 분말을 sol-gel을 이용하여 제조하였다. 결정학적 및 자기적 성질을 x선 회절분석기(XRD), 열분석기(TG/DTA), 적외선분광기(FT/IR), 시료진동형자화율측정기(VSM), 및 Mossbauer 분광기를 이용하여 연구하였다. X선 회절 분석결과 격자상수 $\alpha$와 c는 x=0.0일 때 $\alpha$=5.822, c=23.215 $\AA$에서 x=1.0일 때 $\alpha$=5.895, c=23.295 $\AA$으로 x의 치환량이 늘어남에 따라 증가함을 알 수 있었다. Mossbauer 스펙트럼 분석 결과 $Co^{2+}$- $Ti^{4+}$ 이온의 site 점유도가 포화자화 값과 보자력을 조절할 수 있음을 알 수 있었다. $Co^{2+}$와 $Ti^{4+}$ 의 치환량이 증가함에 따라 Curie 온도는 선형적으로 감소함을 알 수 있었다.

• 대한의용생체공학회:의공학회지
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• 제30권5호
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• pp.444-451
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• 2009

Purpose: The aim of present study is to detect longitudinal alterations of mechanical characteristic determined by bone quality (microarchitecture and degree of mineralization) on femur trabecular bone due to metastatic bone tumor Materials and Methods: Each 6 female SD rats (12 weeks old, approximate 250g) were allocated in SHAM and TUMOR Group. W256 (Walker carcinosarcoma 256 malignant breast cancer cell) was injected into the right femur (intraosseous injection) in TUMOR Group, whereas 0.9% NaCl (saline solution) was injected in SHAM Group. The right hind limbs of all rats were scanned by in-vivo micro-CT to acquire structural parameters, bone mineral density, X-ray attenuation and bone mineralization distribution at 0 week and 4 weeks after surgery. Results: BMD, BV/TV and Tb.N of trabecular bone in TUMOR group were markedly decreased (26%, 11% and 23%) while those in SHAM group were significantly increased (34%, 48% and 11%) (p<0.05). BS/BV, Tb.Sp and SMI in TUMOR group were significantly increased (-16%, 38% and 2%) compared with those in SHAM group (-33%, 12% and -16%) (p<0.05). Additionally, bone mineralization in TUMOR group significantly decreased while those in SHAM group was significantly increased (p<0.05). Conclusion: It is identified that how much bone microarchitecture and mineralization are diminished due to the metastatic bone tumor. The results may be helpful to prediction of fracture risk by metastatic bone tumor.

• 한국안광학회지
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• 제12권1호
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• pp.41-51
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• 2007

두께 및 폭이 균일하지 않고 경사진 한 끝이 고정된 단면이 직사각형인 안경테 다리의 자유단에 수직 힘이 작용하는 경우 탄성체 내 모든 점에서 변위 및 접선 기울기의 연속, 구부림 모멘트의 연속, 전단력의 연속 등 법칙에 근거하여 이러한 불균일 안경테 다리의 변위를 이론적으로 구하는 방정식(모델)을 수립하였다. 베타티탄테 다리의 변형에 대한 실제 측정값과 유도한 이론식에 의한 예측 계산 값을 통계적으로 비교한 결과 상관계수 0.992 및 카이검정 결과 p=0.999로 서로 잘 부합되는 것을 알 수 있었다. 따라서 유도한 모델에 의해 다리의 탄성율 및 두께 폭과 같은 형상 변화에 따른 다리의 변형과 작용하는 힘(압력)의 크기 및 변화가 예측 가능하다. 안경테 다리의 두께 변화에 따른 다리 변형을 모사(simulation)하였다.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.295-298
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• 2008

Creatine kinase (CK; E.C. 2.7.3.2) is an important enzyme that catalyzes the reversible transfer of a phosphoryl group from ATP to creatine in energy homeostasis. The brain-type cytosolic isoform of creatine kinase (BB-CK), which is found mainly in the brain and retina, is a key enzyme in brain energy metabolism, because high-energy phosphates are transfered through the creatine kinase/phosphocreatine shuttle system. The recombinant human BB-CK protein was overexpressed as a soluble form in Escherichia coli and crystallized at $22^{\circ}C$ using PEG 4000 as a precipitant. Native X-ray diffraction data were collected to $2.2{\AA}$ resolution using synchrotron radiation. The crystals belonged to the tetragonal space group $P4_32_12$, with cell parameters of a=b=97.963, $c=164.312{\AA},\;and\;{\alpha}={\beta}={\gamma}=90^{\circ}$. The asymmetric unit contained two molecules of CK, giving a crystal volume per protein mass $(V_m)$ of $1.80{\AA}^3\;Da^{-1}$ and a solvent content of 31.6%.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.59-62
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• 2008

The peptide methionine sulfoxide reductases (Msrs) are enzymes that catalyze the reduction of methionine sulfoxide back to methionine. Because of two enantiomers of methionine sulfoxide (S and R forms), this reduction reaction is carried out by two structurally unrelated classes of enzymes, MsrA (E.C. 1.8.4.11) and MsrB (E.C. 1.8.4.12). Whereas MsrA has been well characterized structurally and functionally, little information on MsrB is available. The recombinant MsrB from Bacillus subtilis has been purified and crystallized by the hanging-drop vapor-diffusion method, and the functional and structural features of MsrB have been elucidated. The crystals belong to the trigonal space group P3, with unit-cell parameters a=b=136.096, $c=61.918{\AA}$, and diffracted to $2.5{\AA}$ resolution using a synchrotron-radiation source at Pohang Light Source. The asymmetric unit contains six subunits of MsrB with a crystal volume per protein mass $(V_M)\;of\;3.37{\AA}^3\;Da^{-1}$ and a solvent content of 63.5%.

• 한국콘텐츠학회논문지
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• 제12권6호
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• pp.317-327
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• 2012

본 연구는 연령층별 사회경제적 계층, 건강행위, 주관적 건강수준 간의 직 간접 경로를 규명하고 그 영향력을 파악하고자 하였다. 제4기 2차년도(2008) 국민건강영양조사에 참여한 성인 중, 교육, 소득, 직업의 3가지 변수로 사회경제적 계층 구분이 가능한 만 25세 이상 4,987명을 연구대상으로 하여 사회경제적 계층을 교육과 소득은 4단계, 직업은 7단계로 분류하고, 건강행위의 실천이 주관적 건강수준에 미치는 영향은 경로분석을 수행하였다. 복합표본분석으로 chi-square test, t-test, one-way ANOVA를 실시하였다. 그 결과 주관적 건강수준은 사회경제적 계층이 낮을수록 낮았으며 청장년층일수록 더 많은 건강행위가 사회경제적 계층에 따라 차이를 보였다. 낮은 사회경제적 계층일수록 주관적 건강수준이 낮아지는데, 청장년층 여자는 금연과 체중조절, 노년층 남자는 운동이 매개변수로써 간접영향을 주었다. 이를 통해 건강증진사업 기획 시 연령층, 성별, 사회경제적 계층을 고려한 정확한 표적집단 선정과 각 표적집단에 적합한 내용 구성이 필요하리라 본다.