• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.7
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• pp.825-831
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• 2007

Anti-proliferation effects of decursin from Angelica gigas Nakai were investigated in the MCF-7 cells treated with environmental hormones. The proliferation was decreased in a dose-dependent manner at the concentration over 20 ${\mu}g/mL$ in the MCF-7 cells treated with decursin of various concentrations. The environmental hormones such as $17{\beta}$-estradiol and bisphenol increased the growth of MCF-7 cells in the charcoal-treated FBS (cFBS) medium and the proliferation was the highest at 0.1 ${\mu}M$ among the tested hormone concentration. Decursin was predicted to inhibit the proliferation in a dose-dependent fashion at tested concentrations (1, 3, 10 or 30 ${\mu}g/mL$) in the MCF-7 cells added environmental hormones; however, the survival rate of the cells was lower than that of control cells that were not treated with decursin at 30 ${\mu}g/mL$ concentration. The chromatin condensation and apoptotic body were examined in the decursin treated cells cultured with the cFBS medium added environmental hormones. These results suggest that decursin decreased the proliferation through apoptosis in the MCF-7 cells added environmental hormones.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.6
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• pp.663-666
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• 2009

Anti-proliferation effects of Corni fructus ethanol extracts were investigated in the RC58T/h/SA#4 cells treated with environmental hormones including dioxin and bisphenol A. The proliferation was decreased at the concentration over $500{\mu}g/mL$ in the RC58T/h/SA#4 cells with ethanol extracts of various concentrations (1, 10, 100, 500, and $1000{\mu}g/mL$). The environmental hormones such as dioxin and bisphenol A increased the growth of RC58T/h/SA#4 cells in the charcoal-treated FBS (cFBS) medium. The proliferation was the highest at 1 nM and $0.1{\mu}M$ for the tested dioxin and bisphenol A concentration, respectively. Ethanol extracts showed inhibition of the proliferation in a dose-dependent manner at the tested concentrations (10, 100, 300, and $500{\mu}g/mL$) in the RC58T/h/SA#4 cells treated with the environmental hormones. The anti-proliferation was the highest at $500{\mu}g/mL$ concentration among the tested ethanol extracts.

• Toxicological Research
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• v.32 no.3
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• pp.215-223
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• 2016

This study is to investigate the biological, biochemical and cytotoxic effects of puffer fish (Arothron stellatus) toxin extracts under in-vitro condition. Extracted toxins from various organs of puffer fish were purified by using active charcoal column, and Bio-gel-P2 column chromatography. The lethality of toxin was tested in crabs, which consists of neurotoxic compounds. The degree of the brine shrimp lethality assay was found directly proportional to the concentration of the toxin extracts, which was well supported by hemolytic assay. The experimental results suggested that the gonad was found higher toxins than the liver and muscles. The mortality rate of brine shrimp nauplii was increased with the raise of concentrations of toxin level. Among the different doses and time dependent cytotoxic effect of human cervical carcinoma (HeLa) cells were showed $4.0{\mu}g/mL$ of toxin, which was effectively inhibited cancer cell proliferation. HPLC and TLC analysis was revealed that the A. stellatus toxin contains tetrodotoxin (TTX), related compounds 4-epi TTX and anhydro-TTX. The present results suggested that the A. stellatus contain TTX as a major and anh-TTX as a minor toxin. It could be the potential candidate in the field of anticancer drug discovery against human cervical cancer cells. The present data is confirming that the puffer fish toxin as an interesting source of novel bioactive natural compounds with potent applications in pharmacology.

• Journal of Korean Society for Atmospheric Environment
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• v.9 no.4
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• pp.310-319
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• 1993

A pilot study was conducted in order to investigate the concentrations of indoor and outdoor VOCs (Volatile Organic Compounds) at ten homes and four building offices in Seoul during March-April, 1993. The five components of VOCs(Benzene, Toluene, Ethylbenzene, o-Xylene, m/p-Xylene) were collected using charcoal tube and were analysed using Gas Chromatography(GC) with a Flame Ionization Detector(FID). The mean concentations of indoor VOCs were shown as Benzene of 38.9 .mu.g/m$^{3}$. Toluene of 165.0 .mu.g/m$^{3}$, Ethylbenzene of 21.7 .mu.g/m$^{3}$, o-Xylene of 11.6 .mu.g/m$^{3}$ and m/p-Xylene of 29.3 .mu.g/m$^{3}$, but those corresponding that indoor levels of VOCs were higher than corresponding outdoor levels. The ratio of indoor and outdoor VOCs were higher than corresponding outdoor levels. The ratio of indoor and outdoor VOCs concentrations was 0.99 for Benzene, 1.23 for Toluene, 5.86 for Ethylbenzene, 5.23 for o-Xylene, 2.41 for m/p-Xylene in homes, while 2.02 for Benzene, 1.15 for Toulene, 0.96 for Ethylbenzene, 1.41 for o-Xylene, 1.38 for m/p-Xylene in offices, respectively. The mean concentrations of VOCs in homes were higher than those levels in offices, while the mean concentration of VOCs during active hour of occupants in a day were higher 1-3 times than the levels during non-active hour. Comparing VOCs levels by building's age, the mean concentrations of Benzene, o-Xylene and m/p-Xylene were higher in new building than old building, but the mean concentrations of Toluene and Etylbenzene in new building were lower than old building. The mean concentrations in all components of VOCs in smoking area were higher than non-smoking area. These results suggested that the VOC levels were affected by various indoor characteristics and behavioral activity of occupants.

• Journal of Korean Society on Water Environment
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• v.26 no.3
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• pp.446-453
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• 2010

This study was investigated nine nitrosamines in small tributary rivers, sewage treatment plants (STPs) and drinking water treatment plants. They are N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosopyrrolidine (NPYR), N-nitrosodi-n-propylamine (NDPA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine (NPIP), N-nitrosodi-n-butylamine (NDBA) and N-nitrosodiphenylamine (NDPHA). The nine nitrosamines were analyzed by gas chromatography mass spectrometry (GC/MS) using solid phase extraction (SPE) with a coconut charcoal cartridge. Among the nine nitrosamines, NDMA, NMEA, NDEA, NDPA NDBA and NDPHA were detected in small tributary rivers and sewage tretment plants. In small tributary rivers, NDMA, NMEA, NDEA, NDPA, NDBA and NDPHA were obtained as ND~16.4 ng/L, ND~17.7 ng/L, ND~102.4 ng/L, ND~455.4 ng/L, ND~330.1 ng/L and ND~161.0 ng/L, respectively. Also NDMA, NMEA, NDEA, NDPA and NDBA were investigated ND~821.4 ng/L, 22.5~55.4 ng/L, 53.2~588.5 ng/L, ND~56.6 ng/L and ND~527.9 ng/L in STPs, respectively. In drinking water treatment plants, NMEA and NDEA concentration were increased to as high as 38.8 ng/L after ozonation process. However nitrosamines were decreased subsequent biological activated carbon (BAC) treatment process. It was supposed that nitrosamines were formed by $O_3$ oxidation and were removed by biodegradation of BAC.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.265-272
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• 2006

This study was conducted to investigate the efficient propagation method of Cyrtomium caryoptideum var. coreanum by sporophyte culture. The influence of origin of explant sources (rhizome, blade, or stipe) and homogenization of culture materials on shoot regeneration were investigated. As a result, only rhizome explant exhibited the organogenic capacity and the shoot regeneration was promoted by homogenization of culture material. Vigorous and excellent growth of multiple shoots was induced on the half-strength of inorganic salts containing MS medium. It was appeared that optimum nitrogen content of shoot regeneration was half-strength of nitrogen containing MS medium (30mM) and optimum sucrose concentration was 1%. Addition of $NaH_2PO_4$ to culture medium generally enhanced shoot multiplication and promoted growth of the regenerants. The organogenic capacity of homogenized rhizomes was especially promoted on medium supplemented with $5{\mu}M$ kinetin plus $5{\mu}M$ IBA. The incorporation of $0.1\sim0.2%$ activated charcoal on medium supplemented with growth regulators prevented the formation of multiple bud primordia - nodule-like bud clusters and improved the normal morphogenesis of sporophytes.

• Asian Journal of Atmospheric Environment
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• v.9 no.2
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• pp.173-186
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• 2015

In Bangladesh, increases in the tropospheric ozone ($O_3$) concentration and in soil salinization may lead to crop damage. To clarify the effects of $O_3$ and/or soil salinity on Bangladeshi wheat cultivars, BAW1059 (salt-tolerant) and Shatabdi (salt-sensitive) were exposed to 70-day treatments with $O_3$ (charcoal-filtered air (CF), $1.0{\times}O_3$, and $1.5{\times}O_3$) and different levels of soil salinity (0, 4, and $8dS\;m^{-1}$). In both cultivars, the whole-plant dry mass and grain yield were significantly reduced by exposure to $O_3$. Increased soil salinity caused significant reductions in whole-plant growth and yield in Shatabdi, but the reductions were negligible in BAW1059. No significant interactions between $O_3$ and salinity were detected for growth, yield, and leaf gas exchange parameters in both cultivars. We concluded that the effects of $O_3$ are not ameliorated by soil salinity in two Bangladeshi wheat cultivars, regardless of their salinity tolerance.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.29 no.4
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• pp.488-499
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• 2019

Objectives: This study was conducted to identify the characterization of organics solvent exposure among painting workers in the shipbuilding painting process, especially for their daily variations(within worker variance). Methods: Charcoal passive dosimeters were worn throughout each work shift for 20 days for five painting workers from October 19 to November 26, 2015. A total of 100 samples were collected, analyzed and compared with statutory workplace environmental measurements. Results: The geometric mean(GM) and geometric standard deviation(GSD) of mixed organic solvent(six substances) exposure index(EI) for the 100 samples were 0.42 and 4.42 respectively. The median and range of GSD for within worker EI representing five workers' daily EI variation is 3.72 and 2.63 ~ 5.20, respectively, which is classified as a very large variation(GSD>3). We were able to divide the painting process into two similar exposure groups(SEGs), Touch-up and Spray. Spray painting workers were much more exposed to organic solvent than Touch-up painting workers(GM=0.71 vs. 0.19), but less variably (GSD=3.64 vs. 4.10). xylene is the substance to which the workers were most exposed(GM=16.19 ppm, GSD=4.36), and the exposure characteristics of six substances including xylene is similar to those of EI. Conclusions: The daily variation of organic solvent exposure in the shipbuilding painting process is so high that statutory Assessment of Reliability of Work Environment Monitoring needs to be conducted with statistically sufficient number of samples and evidence.

• Korean Journal of Environmental Agriculture
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• v.2 no.2
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• pp.103-107
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• 1983

The effect of photochemical oxidants on rice plants was measured by growing the rice plants Nihonbare in pot in charcoal-manganese oxide filtered atmosphere and non-filtered air. Visible injury on the leaf blades of rice plants were observed in plants grown under the unfiltered air chamber, but plants under filtered air chamber were free from any injury. Fresh weight of stem and root at maximum tillering stage in unfiltered chamber were 16.8 and 46.4% less than filtered air chamber, respectively. Grain yield in unfiltered air chamber was also reduced by 14.7% compared to that of filtered air chamber. And the reduced yield paralleled increase in concentration of oxidants in the atmosphere at the experimental site. ABA content in rice plants cultivated in unfiltered air chamber was higher than in filtered air chamber, but the root activity of rice plants in unfiltered air chamber was remarkably decreased.

• Korean Journal of Agricultural Science
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• v.43 no.3
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• pp.340-345
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• 2016

The kiwi fruit (Actinidia deliciosa cv. 'Hayward') should be ripened at any step during postharvest handling before consumer consumption. This is essential for freshly harvested kiwi fruit. But, this requires correct temperatures and ethylene concentrations. More testing of a newly developed ethylene generator using charcoal for commercial purposes is needed. This study was conducted to investigate the optimum storage temperatures and the effect of ethylene on the postharvest quality of kiwi fruit. Three different ethylene concentrations of 10, 50, and $100{\mu}L{\cdot}L^{-1}$ were used on fresh kiwi fruit stored at different temperatures of 10, 15, and $20^{\circ}C$. The quality changes of the fruits were assessed by sensory evaluation and by measuring firmness, soluble solids content, titratable acidity, and ethylene production. Higher storage temperatures and ethylene concentrations softened the kiwi fruit quickly and led to the rapid loss of acidity while soluble solid contents of fruit increased to a significant extent during the same storage period. Similarly, the firmness of ethylene-treated fruits stored at 20 and $15^{\circ}C$ dramatically decreased in the experiment while treated fruits stored at $10^{\circ}C$ decreased only slightly. Quality characteristics of kiwi fruits stored at 15 and $20^{\circ}C$ were better than those of fruits at $10^{\circ}C$. With regards to the effect of temperature, fruits stored at lower temperatures took a longer time to ripen and retained their quality longer. The newly developed ethylene generator maintained the ethylene concentration in the 5 kg box at $40-400{\mu}L{\cdot}L^{-1}$. The ethylene generator could also be used to soften persimmons.