• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7687-7692
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• 2014

Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore the potential mechanism of ATPR inhibiting of A549 cell migration. Materials and Methods: The cytotoxicity of ATRA and ATPR on A549 cells was assessed using MTT assay. Wound healing assays were used to analyze the influences of ATRA, ATPR, ML-7 (a highly selective inhibitor of myosin light chain kinase (MLCK)), PMA (an activator of MAPKs) and PD98059 (a selective inhibitor of ERK1/2) on the migration of A549 cells. Expression of MLCK and phosphorylation of myosin light chain (MLC) were assessed by Western blotting. Results: ATRA and ATPR inhibited the proliferation of A549 cells in a dose- and time-dependent manner, and the effect of ATPR was much more remarkable compared with ATRA. Relative migration rate and migration distance of A549 cells both decreased significantly after treatment with ATPR or ML-7. The effect on cell migration of PD98059 combining ATPR treatment was more notable than that of ATPR alone. Moreover, compared with control groups, the expression levels of MLCK and phosphorylated MLC in A549 cells were both clearly reduced in ATRA and ATPR groups. Conclusions: ATPR could suppress the migration and invasion of A549 cells, and the mechanism might be concerned with down-regulating the expression of MLCK in the ERK-MAPK signaling pathway, pointing to therapeutic prospects in lung cancer.

• 한국IT서비스학회지
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• 제12권3호
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• pp.1-21
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• 2013

This study attempts to explain the mechanism behind the migration from vertically integrated value chain architecture to an innovation ecosystem consisting of horizontally separated layers in value chain. We first present a comprehensive framework based on the theoretical analysis of the drivers for migration to an innovation ecosystem, which are standard (institution), capability distribution, and transaction costs. The theoretical framework suggests that the migration to an innovation ecosystem is explained by the influence of standard on the evolutionary change of capability distribution and transaction costs. In particular, when the new de-jure standard competes with the de-facto standard, the new de-jure standard has the greatest impact on the distribution capabilities and the transaction costs. Based on this theoretical framework, we analyze the latest SDN (Software Defined Networking) case of the network industry. SDN standard has transformed the industry from a vertically integrated value chain architecture to a horizontally separated one with its influence on the distribution capabilities and the transaction costs in the industry.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권8호
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• pp.2783-2804
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• 2021

In the network function virtualization environment, dynamic changes in network traffic will lead to the dynamic changes of service function chain resource demand, which entails timely dynamic adjustment of service function chain resource configuration. At present, most researches solve this problem through virtual network function migration and link rerouting, and there exist some problems such as long service interruption time, excessive network operation cost and high penalty. This paper proposes a dynamic adjustment method of service function chain resource configuration for the dynamic changes of network traffic. First, a dynamic adjustment request of service function chain is generated according to the prediction of network traffic. Second, a dynamic adjustment strategy of service function chain resource configuration is determined according to substrate network resources. Finally, the resource configuration of a service function chain is pre-adjusted according to the dynamic adjustment strategy. Virtual network functions combination and virtual machine reusing are fully considered in this process. The experimental results show that this method can reduce the influence of service function chain resource configuration dynamic adjustment on quality of service, reduce network operation cost and improve the revenue of service providers.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5835-5842
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• 2015

Background: Melatonin, which is mainly produced by the pineal gland, has a good inhibitory effect on cell growth of multiple cancer types. However, the underlying molecular mechanisms of anti-tumor activity for colon cancer have not been fully elucidated. In this study, we investigated the effects of melatonin on migration in human colon cancer RKO cells and the potential molecular mechanisms. Materials and Methods: The viability of RKO cells was investigated by MTT assay after treatment with melatonin, SB203580 (p38 inhibitor) and phorbol 12-myristate 13-acetate (PMA, MAPK activator) alone or in combination for 48h. The effects of melatonin, and ML-7, a selective inhibitor of myosin light chain kinase (MLCK), and SB203580, and PMA on the migration of RKO cells were analyzed by in vitro scratch-wound assay. The relative mRNA levels of MLCK was assessed by real-time quantitative RT-PCR. Western blotting analysis was performed to examine the expression of MLCK, phosphorylation of myosin light chain (pMLC) and p38 (pp38). Results: The proliferation and migration of human colon cancer RKO cells were inhibited significantly after treatment with melatonin. The expression levels of MLCK and phosphorylation of MLC of RKO cells were reduced, and real-time quantitative RT-PCR showed that melatonin had significant effects on suppressing the expression of MLCK. Furthermore, the phosphorylation level of p38, which showed the same trend, was also reduced when cells were treated by melatonin. In addition, ML-7 (25umol/l) could down-regulate the phosphorylation of p38. Conclusions: Melatonin could inhibit the proliferation and migration of RKO cells, and further experiments confirmed that p38 MAPK plays an important role in regulating melatonin-induced migration inhibition through down-regulating the expression and activity of MLCK.

• 한국임상수의학회지
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• 제38권6호
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• pp.261-268
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• 2021

The study was conducted to evaluate the effects of carboxymethyl chitosan (CMC) on proliferation, migration, and lipopolysaccharide (LPS)-induced inflammatory response in canine bone marrow-derived mesenchymal stem cells (BMSCs). The proliferation and migration of BMSCs were examined after treatment with CMC. The effect of CMC on the mRNA expression of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, IL-10, and transforming growth factor (TGF)-β, was also evaluated by reverse transcription polymerase chain reaction (RT-PCR). In the proliferation assay, no significant changes were found at all CMC concentrations compared with controls. The migration assay showed that CMC dose-dependently stimulated the migration of BMSCs in normal and LPS-treated conditions. RT-PCR showed that TNF-α and IL-10 expressions were suppressed in the BMSCs after CMC treatment. However, other genes were not affected. Taken together, CMC promoted BMSC migration and inhibited TNF-α and IL-10. Therefore, CMC may be possible to regulate wound healing when mesenchymal stem cells are applied in inflammatory diseases.

• 한국염색가공학회지
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• 제18권4호
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• pp.28-36
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• 2006

The polyurethane prepolymers, which were previously synthesized from 2,4-toluene disocyanate(2,4-TDI) and polypropylene glycol(PPG), were chain extended by ethylene diamine or hydroxyl terminated polydimethylsiloxane(HTPMS) having hydroxy group at both ends of the chain, giving polyurethaneurea(PU) and polyurethane containing HTPMS segment(SiPU), respectively. In thermal gravimetric analysis, PU was almost completely degraded at $500^{circ}$ but SiPU showed about 11% residue at the same temperature. Suspension of SiPU and pigment showed more good compatibility than that of PU and pigment. The crocking fastness, migration fastness and solvent wicking were enhanced to 4.5 grades, 4 grades and 4 grades, respectively.

• Nuclear Engineering and Technology
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• 제26권4호
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• pp.453-460
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• 1994

암반(Porous Rock Matrix)과 균열(fracture)에서 일차원의 이동 방정식(Migration Equation)을 3-Member Decay Chain까지 화장하고, Laplace Transform을 이용하여 초기조건이 Delta Function과 Bateman Equation인 각각에 대해 해석해를 구한다. 그 해를 이용하여 Actinide Chain 중 4n＋1과 4n＋2 Chain에서 선택된 Np$^{241}$-U$^{233}$ -Th$^{229}$ 와 U$^{234}$ -Th$^{230}$ -Ra$^{226}$ Chain의 각 핵종들의 균열에서의 농도를 상대농도로 나타낸다. 이핵종들의 지연계수(Retardation Coefficient)는 화강암에 대한 것을 사용하여 균열에서의 농도 변화를 볼 수 있다. 본 연구에 의한 결과로는 U$^{233}$ , Th$^{229}$ , Th$^{230}$ Ra$^{226}$ 같은 핵종들은 비록 초기 inventory에는 작은 양일지라도 균열과 암반에서 모핵종의 붕괴(decay)에 의해 생기므로써 처분장으로부터 먼 거리에서는 중요한 핵종이 된다는 것을 알 수 있다.

• 대한지리학회지
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• 제51권4호
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• pp.525-539
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• 2016

본 연구는 사후분포를 예측하는 베이지안 추정기법의 일환인 마르코프 체인 모형을 적용하여 직업요인 인구이동에 따른 직종별 취업자의 공간적 분포에 나타나는 변화를 예측하였다. 이를 위해 인구이동의 사유 중 직업요인 이동량을 추출하여 직업을 요인으로 하는 인구이동 패턴을 파악하고, 직업요인 인구이동의 추이확률 산출 값을 토대로 채프만-콜모고로프 방정식을 구축하여 장래 지역별 취업자 분포와 직종분포의 변동성을 예측하였다. 분석결과, 서울의 취업자 분포가 감소할 것으로 예측되나 직종 중 단순노무 종사자는 증가할 것으로 예측되었다. 전문가 및 관련 직의 경우 수도권과 일부 광역시를 제외한 모든 지역에서 증가할 것으로 추정되었고, 강원, 충청지역은 전체 직업군의 취업자 분포에 있어 증가세를 나타낼 것으로 예측되었다. 본 연구 결과는 향후 지역 노동시장의 원활한 인력수급이 가능하도록 유입, 유출될 가능성이 높은 인력 및 직종을 중심으로 직업훈련, 취업알선 등 고용지원 서비스를 통해 사전 대비하는 방안 마련에 기초자료로 활용될 수 있다.

• The Plant Pathology Journal
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• 제28권2호
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• pp.191-196
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• 2012

Periwinkle seedlings (Cantharanthus roseus) were inoculated with jujube witches'- broom (JWB) phytoplasma via grafting to analyze the migration of JWB phytoplasmas within the host plant. The phytoplasmas were detected using nested polymerase chain reaction (PCR) and fluorescence microscopy. Fluorescence microscopy was a simple and easy method of detecting phytoplasmas; however, it was not sufficiently sensitive to detect very low phytoplasma concentrations. Therefore, the migration of JWB phytoplasma was investigated through PCR. The first migration of JWB phytoplasma from an infected tissue to healthy tissues occurred late. After grafting, the phytoplasmas moved from the inoculated twig (or scion) to the main stem, which took 28 days. Afterward, the phytoplasma migrated faster and took less than 4 days to spread into the roots from the main stem. All twigs were then successively colonized by the JWB phytoplasmas from the bottom to the top. JWB phytoplasma was detected via nested PCR in all parts of the periwinkle seedling 82 days after inoculation. Based on these results, the inoculated JWB phytoplasma appeared to migrate downward to the roots along the main stem during the early stages, and then continued to move upward, colonizing twigs along the way until they reached the apex.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7265-7270
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• 2013

Objective: The present study was designed to investigate the probable mechanisms of synthetic retinoid 4-amino-2-tri-fluoromethyl-phenyl ester (ATPR) inhibition of the proliferation and migration of A549 human lung carcinoma cells. Materials and Methods: After the A549 cells were treated with different concentrations of ATPR or all-trans retinoic acid (ATRA) for 72 h, scratch-wound assays were performed to assess migration. Immunofluorescence was used to determine the distribution of CAV1 and $RXR{\alpha}$, while expression of CAV1, MLCK, MLC, P38, and phosphorylation of MLC and P38 were detected by Western blotting. Results: ATPR could block the migration of A549 cells. The relative migration rate of ML-7 group had significantly decreased compared with control group. In addition, ATPR decreased the expression of a migration related proteins, MLCK, and phosphorylation of MLC and P38. ATPR could also influence the expression of RARs or RXRs. At the same time, CAV1 accumulated at cell membranes, and $RXR{\alpha}$ relocated to the nucleus after ATPR treatment. Conclusions: Caveolae may be implicate in the transport of ATPR to the nucleus. Change in the expression and distribution of $RXR{\alpha}$ may be implicated in ATPR inhibition of A549 cell proliferation. The mechanisms of ATPR reduction in A549 cell migration may be associated with expression of MLCK and phosphorylation of MLC and P38.