• BMB Reports
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• v.44 no.5
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• pp.298-305
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• 2011

Most intracellular reactive oxygen species (ROS), especially superoxide anion ($O_2^{{\bullet}_-}$) that is converted from oxygen, are overproduced by excessive electron leakage from the mitochondrial respiratory chain. Intracellular oxidative stress that damages cellular components can contribute to lifestyle-related diseases such as diabetes and arteriosclerosis, and age-related diseases such as cancer and neuronal degenerative diseases. We have previously demonstrated that the excessive mitochondrial $O_2^{{\bullet}_-}$ production caused by SDHC mutations (G71E in C. elegans, I71E in Drosophila and V69E in mouse) results in premature death in C. elegans and Drosophila, cancer in mouse embryonic fibroblast cells and infertility in transgenic mice. SDHC is a subunit of mitochondrial complex II. In humans, it has been reported that mutations in SDHB, SDHC or SDHD often result in inherited head and neck paragangliomas (PGLs). Recently, we established Tet-mev-1 conditional transgenic mice using our uniquely developed Tet-On/Off system, which equilibrates transgene expression to endogenous levels. These mice experienced mitochondrial respiratory chain dysfunction that resulted in $O_2^{{\bullet}_-}$ overproduction. The mitochondrial oxidative stress caused excessive apoptosis leading to low birth weight and growth retardation in the neonatal developmental phase in Tet-mev-1 mice. Here, we briefly describe the relationships between mitochondrial $O_2^{{\bullet}_-}$ and aging phenomena in mev-1 animal models

• Journal of Nutrition and Health
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• v.16 no.1
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• pp.10-20
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• 1983

In order to establish tissue lipid status in animal on feeding of various dietary fat and oils, each group of rats was fed a semisynthetic diet containing 10%(w/w) mackerel oil (MO), eel oil (EO), soybean oil (SO), rapeseed oil (RO) or beef tallow (BT) for 1, 2 and 4 weeks, After each feeding period, levels of cholesterol, triglyceride and phospholipid were measured in serum. Fatty acid ${\leftarrow}$ composition was also investigated in serum and tissue lipids. Levels of total serum cholesterol were lower but HDL-cholesterol were higher in fish oil groups, which resulted in significantly higher ratio of HDL to total cholesterol in the fish oil groups. Fish oil groups, in general, also had lower levels of serum triglyceride and phospholipid than other groups, but S0 group maintained as low phospholipid levrl as fish oil groups. Fatty acid composition of dietary fat was reflected in all the tissues investigated but with varying degrees. Very long chain fatty acids, specific components exclusively found in fish oils were most well reflected in liver and relatively well in serum, whereas linoleic acid and erucic acid of SO and RO in the diet were better shown up in heart and adipose tissue. It attracted a particular attention that major proportions of long chain monoenoic acids $(C_{22:1})$ occurring both in MO and RO were detected separately in liver and heart plus adipose tissue, the result of which strongly indicates that there is a significant difference in metabolism between isomers $(C_{22:1}\;w\;11\;and\;C_{22:1}\;w\;9,\;respectively)$. It is suggested from this study that differences in lipid status as well as in the levels of serum lipids result from uniqueness in metabolism of each different fatty acid and give rise to distinguishable change in serum lipoprotein pattern, followed by diet with different fat sources.

• Journal of Biosystems Engineering
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• v.36 no.1
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• pp.9-14
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• 2011

This study was carried out to develop a vine crusher for harvesting sweet potato. The experimental two-row vine crusher attachable to agricultural tractor composed of vine crushing part with frail type vine crushing blades and vine lifting blades, power transmission part with chain and gear transmission mechanism, crushing height control part with two control wheels and manual levers, and implement frames, was designed and fabricated. And this vine crushing performance was also analyzed. From vine crushing tests, backward travel direction (i.e., rotational direction of the vine crushing blades) showed better vine crushing performance than forward travel direction. Crushing ratio of remained vine was increased, and length of remained vine and length of crushed vine were decreased as working speed was decreased and rotational speed of vine crushing blades was increased. At a working speed of 0.27 m/s and rotational speed of vine crushing blades of 800 rpm, crushing ratio of remained vine was 98%, length of remained vine was 104 mm, and length of crushed vine was 327 mm. But, when crushing vine on irregular ridges, vines and mulching vinyl were wound in the vine crushing part. Therefore, change of location of power transmission chain mechanism, and an automatic control device for controlling crushing height were needed.

• Molecules and Cells
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• v.37 no.8
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• pp.598-604
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• 2014

Fatty acids, important components of a normal diet, have been reported to play a role in bone metabolism. Osteoclasts are bone-resorbing cells that are responsible for many bone-destructive diseases such as osteoporosis. In this study, we investigated the impact of a medium-chain fatty acid, capric acid, on the osteoclast differentiation, function, and survival induced by receptor activator of NF-${\kappa}B$ ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Capric acid inhibited RANKL-mediated osteoclastogenesis in bone marrow-derived macrophages and suppressed RANKL-induced $I{\kappa}B{\alpha}$ phosphorylation, p65 nuclear translocation, and NF-${\kappa}B$ transcriptional activity. Capric acid further blocked the RANKL-stimulated activation of ERK without affecting JNK or p38. The induction of NFATc1 in response to RANKL was also attenuated by capric acid. In addition, capric acid abrogated M-CSF and RANKL-mediated cytoskeleton reorganization, which is crucial for the efficient bone resorption of osteoclasts. Capric acid also increased apoptosis in mature osteoclasts through the induction of Bim expression and the suppression of ERK activation by M-CSF. Together, our results reveal that capric acid has inhibitory effects on osteoclast development. We therefore suggest that capric acid may have potential therapeutic implications for the treatment of bone resorption-associated disorders.

• Korean Journal of Microbiology
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• v.31 no.4
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• pp.261-266
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• 1993

A new methyltrophic bacterium which utilizes methanol as a sole source of carbon and energy was isolated from soil. It was Gram-negative, nonmotile, nonspore-forming rod, and strictly aerobic bacterium. Catalase and oxidase activities were present. Nitrate was reduced to nitrite. Vitamins and other growth factors were not required. Generation time was 1.6 hr under the optimal condition. The isolate assimilated methanol via the ribulose mono-phosphate pathway (Enter-Doudoroff varient) and did not have .alpha.-ketoglutarate dehydrogenase. It assimilated ammonia through glutamate dehydrogenase. The guanine plus cytosine content of the DNA was 61.0 mol%. The celular fatty acid composition was primarily straight-chain saturated $C^{16 : 0}$ acids (palmitic acids) and unsaturated $C_{16 :1}$ acid (palmitoleic acids), and the isolate also contained two unidentified $C_{17}$ branched fatty acids. The major ubiquinone was Q-8, and Q-6 and Q-7 were present as minor components. Phosphatidylethanolamine and phosphatidylglycerol were predominantly present, and diphosphatidyglycerol was also detected. Based on the physiological and biochemical properties, the isolate was assigned to a novel species of the genus Methylobacillus, Methylobacillus methanolovorus sp. nov.

• Journal of Adhesion and Interface
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• v.12 no.4
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• pp.105-110
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• 2011

Polyester/melamine cured coatings had been used for pre-primed coatings and pre-coated metal coatings, because it has good mechanical,chemical properties, and mar resistance. But it has weak points such as stiffness and low formability for making automotive components. Polyester had been synthesized using polycarbonate diol of long alkyl chain which can improve flexibility and formability which is one of the important factors for pre-coated steel sheets (PCM). In this study, strain and tensile strength were examined by the tensile test and formability was examined by the drawing test. Also, Those polyester resins were also measured by DMA to verify flexibility of cured coatings.

• Korean Journal of Construction Engineering and Management
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• v.15 no.4
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• pp.20-29
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• 2014

As construction projects increase their complexity, variety, and scale, various recognition applications (such as RFID, bar-code etc.) have been tried for managing material effectively in construction projects. However, existing recognition applications for construction material management have some limitations that cause additional works (such as attaching RFID tag), additional cost (labor cost, recognition device cost, etc.), and cognitive impairment of workers. Therefore, this study proposed a character recognition technology as an alternative of previous recognition technologies such as RFID, bar-code, etc. The technical feasibility of proposed technology was validated by three recognition tests. Additionally, this study proposed code the structure to manage materials using the character recognition technology. The effects of character recognition technology are presented by comparing with existing RFID-based logistics processes.

• The Korean Journal of Mycology
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• v.29 no.2
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• pp.104-109
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• 2001

The antifungal effects of polyphosphates on growth of Candida albican and Trichophyton mentagrophytes were studied. The polyphosphates with chain length of 15, 45, and 75 were inhibitory to growth of fungi whereas no inhibition was shown by pyrophosphate. As chain length increase, the more inhibitory effect of the polyphosphates on fungal growth was observed. The concentration of polyphosphate at $800\;{\mu}g/ml$ completely inhibited the growth of fungus. Supplementation of the medium with $Mg^{2+}\;and\;Ca^{2+}$ reduced inhibitory effect of polyphosphate on growth of C. albican treatment of C. albican with polyphosphate, the release of nucleic acid out of cell was observed. When C. albican exposed to polyphosphate were examined, profound changes of cell morphology such as cell swelling and surface blebs were observed. In addition, propidium iodide, membrane impermeable dye, stained the nucleus of C. albican cell treated with polyphosphate. Therefore, it is proposed that the antifungal activity of polyphosphate might be related with its chelation effect to essential cation components of fungal cell wall or membrane.

• Journal of KIISE:Software and Applications
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• v.26 no.11
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• pp.1298-1305
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• 1999

네트워크 및 분산 처리 기술의 발달로 분산 환경에서 동작하는 응용 프로그램에 대한 생산성 향상이 요구되고 있다. 본 논문에서는 MVC(Model, View, Controller) 패턴을 기반으로 하는 분산 응용 프로그램의 생산성 향상을 위해, 조립식 분산 응용의 작성을 지원하는 분산 객체 조립기, DOC(Distributed Object Composer)를 소개한다. 분산 객체 조립성의 기반이 되는 DOC의 구성적{{) '전체'를 창조해 내기 위해 여러 요소들을 결합, 배치하여 하나의 예술작품을 성립시키는 방법. 모아서 조립한다는 의미.}} 인터페이스(Structural Interface)는 기존 객체지향 기술의 절차적 인터페이스(Procedural Interface)에 비해 콤포넌트 재사용성을 개선하고, 분산 응용의 신속한 개발을 지원하여, 소프트웨어의 생산성 및 확장성을 향상시킨다. 또한, DOC 방식으로 모델링하고 구현한 공급망 관리(Supply Chain Management: SCM) 응용 프로그램의 조립식 작성을 통해, DOC의 실시간 정보 처리 및 조립 기능을 보인다.DOC는 이질적인 통신 환경에서 클라이언트와 서버 객체간의 네트워크 투과성을 제공하는 미들웨어, CORBA를 기반으로 한다. 또한, 분산 객체들 간의 상호작용을 자동 처리하기 위해 Java의 관찰자/피관찰자(Observer/Observable)를 확장한 분산 관찰자/피관찰자 패턴을 정의하여 DOC와, 서버 및 클라이언트의 분산 응용 객체들을 정의하기 위한 인터페이스에 적용한다.Abstract Due to the availability of advances in network and distributed processing technologies, the enhanced productivity of application software that runs on distributed environment is demanded. In this paper, we introduce the Distributed Object Composer (DOC) that supports the structural composition of distributed applications for the enhanced productivity of MVC (Model, View, Controller) pattern-based application programs. The structural interface of DOC that is the basis for the composibility of distributed objects increases the reusability of software components and provides better software productivity and extendibility than the procedural interface of the traditional object-oriented technology by supporting rapid constructions of distributed applications. In this paper, we also discuss the real-time information processing and composibility features of DOC by applying the DOC mechanism to the Supply Chain Management (SCM) application.DOC is implemented using the CORBA middleware that supports the network transparency between client and server objects on heterogeneous communication environments. We apply the distributed Observer/Observable pattern that extends the Observer/Obseverable of Java to DOC itself and distributed client/server objects as their interface definitions for the automatic interactions of distributed objects.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.8
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• pp.2900-2922
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• 2021

In this paper, the power spectral density(PSD) for Multilevel Minimum Shift Keyed signal with modulation index h = 1/2 (M-ary MSK) are derived using the mathematical method of the Markov Chain model. At first, according to an essential requirement of the phase continuity characteristics of MSK signals, a complete model of the whole process of signal generation is built. Then, the derivations for autocorrelation functions are carried out precisely. After that, we verified the correctness and accuracy of the theoretical derivation by comparing the derived results with numerical simulations using MATLAB. We also divided the spectrum into four components according to the derivation. By analyzing these figures in the graphic, each component determines the characteristics of the spectrum. It is vital for enhanced spectral characteristics. To more visually represent the energy concentration of the main flap and the roll-down speed of the side flap, the specific out-of-band power of M-ary MSK is given. OMLCD(Optimum Maximum Likelihood Coherent Detection) of M-ary MSK is adopted to compare the signal received with prepared in advance in a code element T to go for the best. And M-ary MSK BER(Bit Error Rate) is compared with the same ary PSK (Phase Shift Keying) with M=2,4,6,8. The results show the detection method could improve performance by increasing the length of L(memory inherent) in the phase continuity.