• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.1
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• pp.71-74
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• 2005

A LIM protein gene homologue of the CRP (cysteine­rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypep­tide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRPl). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92$\%$ identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.

• Korean journal of applied entomology
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• v.30 no.3
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• pp.174-179
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• 1991

Patterns of ginseng damage by larvae of Holotrichia diomphalia Bates in spring were investigated in 3rd year ginseng fields from 1986 to 1990. Ginseng damages were more serious in outer areas than inner areas of ginseng fields, which seemed to be due to nocturnal migration behavior of adult females. Damaged parts of 3rd year ginseng plants due to 3rd instar larvae were top, middle, bottom or whole of main roots, and rarely underground stems. Mean inhabitation depth of 3rd instar larvae were 3.2cm, 5.6cm, 6.6cm and 4.9cm in 1st, 2nd and 3rd year ginseng fields and mugwort fields, respectively. Larval feeding damage of ginseng in spring oc¬curred mainly from mid-May to early July and appeared to decrease temporarily when rainfalls increased.

• International Journal of Industrial Entomology and Biomaterials
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• v.44 no.2
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• pp.44-54
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• 2022

The food source of white-spotted flower chafer, Protaetia brevitarsis is important to economical performance. The P. brevitarsis larva were feed by mulberry and oak fermented sawdust. The effect of additional additives, Korean black raspberry (called bokbunja) marc and blueberry marc to fermented sawdust feed on the development and oviposition characteristics were investigated. The development periods of P. brevitarsis was decreased from 165.7 d to 40.0 d (mulberry sawdust) and 169.3 d to 39.0 d (oak sawdust), but the survival rate of larva was not affected by the addition of Korean black raspberry marc to fermented sawdust. However, blueberry marc addition to the fermented sawdust did not significant effect on the development period and survival rate of P. brevitarsis. The percentage of larva weight over 2.5 g was tend to increase by addition of Korean black raspberry marc and/or blueberry marc. The number of oviposition was also tend to increase by adding additional feed to sawdust but there was no significant different.

• International Journal of Industrial Entomology and Biomaterials
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• v.31 no.1
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• pp.25-29
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• 2015

The white-spotted chafer, Protaetia brevitarsis (Coleoptera: Scarabaeidae), has been traditionally used in Korea as a medicine for preventing liver-related diseases and suppressing liver cancer. Therefore, this insect is economically important and is commercially reared and sold in Korea. Recently, P. brevitarsis was listed as a temporal food ingredient by the Korean Ministry of Food and Drug Safety. Given the increasing economic importance of this beetle, we have sought to improve rearing conditions for its commercial production. In this study, we compared the effects of two food supplements, clay and charcoal, on the growth of second instar larvae of P. brevitarsis. Clay and charcoal are generally known as good adsorbent for removal of contaminating substances in insect feed. We fed second instar P. brevitarsis larvae a commercial diet consisting of fermented sawdust with seven different combinations of clay and/or activated charcoal, and measured their effects on weight gain for approximately 17 wk until larvae pupated. We found that addition of clay at 2.5% w/w of the fermented sawdust diet had no negative effect on weight gain of second instar P. brevitarsis larvae and thus may improve the quality of P. brevitarsis as a commercial food.

• Korean Journal of Organic Agriculture
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• v.26 no.2
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• pp.259-268
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• 2018

The study investigated the efficacy of four different essential oils on the repellent responses of large black chafer (Holotrichia parallela) Motschulsky (Coleoptera: Scarabaeidae) in organic pear (Pyrus pyrifolia) orchards. Cinnamon, pine, peppermint, and eucalyptus oils were used, and the behavioral responses and repellent effects against H. parallela were investigated under laboratory and field conditions. Adult beetle responses to different oils were examined using a Y-tube olfactometer in the laboratory and four absorbent blocks with each oil in the field. The repellent responses rates of H. parallela were 100% for cinnamon oil; however, only 67% of adult beetles avoided peppermint and eucalyptus oil in the Y-tube olfactometer bioassay. In the field tests, the least damage to leaves was observed on trees treated with cinnamon oil, whereas the most damage was observed in the control (non-treated) trees and those treated with peppermint oil, followed by eucalyptus and pine oil. Therefore, cinnamon oil can be used as a repellent to avoid damage form large black chafers in organic pear orchards.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.469-472
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• 2002

Solid state fermentation was performed for the production of entomopathogenic fungus Metarhizium anisopliae HY-2 using wheat bran media containing rice bran. Fungal growth in a solid state fermentation system was estimated by viable cell count, spore count, and mycelial biomass. It was used chemical method measuring N-acetyl-glucosamine (chitin) content for estimating of mycelial biomass. In static flask culture, viable cell reached 2.40 ${\times}$ $10^8$ cfu/g at 23 days of culture at $27^{\circ}C$ and then mycelial biomass was 41.59 mg/g. Specific growth rate(${\mu}$ max) was 0.0418 $h^{-1}$ between 3 and 9 days when estimated by viable cell count and was 0.00976 $h^{-1}$ between 9 and 17 days when N-acetylglucosamine content was measured. Viable cells reached 1.12 ${\times}$ $10^8$ cfu/g in polypropylene-bag at 28 days of culture at $27^{\circ}C$. Formulated microbial pesticide containing M. anisopliae HY-2 were tested their bio-activity against Chestnut Brown Chafer (Adoretus tenuimaculatus). The protection rate of the liquid culture showed 13 ${\sim}$ 26 % with 1st to 3rd instar, and spore suspension of M. anisopliae HY-2 showed 56 ${\sim}$ 64%. Conidia produced by large scale solid-state fermentation showed 20 ${\sim}$ 27 % activity 60 ${\sim}$ 64 % with M. anisopliae HY-2.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.5
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• pp.1337-1343
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• 2005

The hepato-protective effects of the extract from Protaetia brevitarsis against hepatotoxicity by carbon tetrachloride ($CCI_4$) were studied in rats. The rats were orally treated with $CCI_4$ (50% in corn oil) at initial dose of $1\;m{\ell}/kg$ followed by $0.5m{\ell}/kg$ four times during 2-week period. The extract of P. brevitarsis (50, 100 or 200 mg/kg) or its vehicle was administered day after day from 1 week before $CCI_4$ Injection during five weeks. $CCI_4$ induced hepato-celluar degeneration and necrosis induced to increase in serum aspartate amintransferase (AST) and alanine aminotransferase (ALT) levels. In biochemical analyses, thiobarbituric acid-reactive substances (TBARS) and antioxidant enzymes such as superoxide dismutase (SOD) and catalase in hepatic tissues were remarkably increased by $CCI_4$ treatment. Not only increases in serum AST and ALT, but also induction of lipid peroxidation and antioxidant enzymes in hepatic tissues caused by $CCI_4$ were significantly attenuated by the P. brevitarsis extract in a dose-dependent manner. Such hepato-protective effects of P. brevitarsis extract were confirmed by histopathological examinations, wherein only mild hepatocytic vacuolations were observed in the liver of rats treated with a high dose (100 mg/kg) of P. brevitarsis extract in comparison with severe hepatocytic degenerations administered with $CCI_4$ alone. From these results, it is suggested that the extract of Protaetia brevitarsis could be a promising candidate for the protection of liver injury, based on the preventive effects against morphological cellular injuries, lipid peroxidation and serum biochemical parameters.

• Korean journal of applied entomology
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• v.32 no.4
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• pp.414-419
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• 1993

Species and seasonal occurrences of Sencinae, Melolonthinae and Rutelinae chafers captured b by blacklight traps were investigated from 1989 to 1991 in Suwon and Chonju. Numbers of s species captured in Suwon and Chonju were 6 and 6 for Sericinae, 8 and 7 for Melolothinae, 7 a and 8 for Rutelinae, 21 and 21 in total, respectively. Species with more captures from both districts in each subfamily were Maladera orientalis of Sericinae, Holotrichia morosa and H. diomphalia of Melolonthinae, and Anomala corpulenta, and A. rufocuprea of Rutelinae. Adults of t the above five species were captured 1O~20 days earlier in Chonju than in Suwon. H. d diomphalia adults occurred mamly In odd years in Chonju, which is the same pattern as in S Suwon.

• Korean journal of applied entomology
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• v.31 no.2
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• pp.170-173
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• 1992

Phytophagous insects of Monocotyledoneae were surveyed from May to October in 1991. Each p province was divided into 9 localities by $\boxplus$-shape for the collection. Seventeen weed species b belonging to 17 genera were collected, namely, 14 Graminales weed species of 14 genera, 1 s species of Farinales weed, and 1 species of Liliales weed. Phytophagous insects representing 5 orders, 13 families, 27 genera, and 36 species comprised the insect fauna on Monocotyledoneae w weeds. Five species were not identified out of 36 insect species. Grasshoppers, hemipteran, s scarab beetles, and leaf beetles were frequently collected. Lema concinnipennis was a potential biological agent on Commelina communis, and Oulema dilutipes was first collected from Setaria u viridis. Commelina communis in golf course was severely damaged by Adoretus tenuimacu/atus c chafer but this insect was also an important insect pest of turfgrass and trees.

• Korean journal of applied entomology
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• v.45 no.2 s.143
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• pp.169-172
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• 2006

Sex pheromone blends of large black chafer, Holotrichia parallela (Motschulasky), which consist of a major component L-isoleucine methyl ester (LIME) and a minor component (R)-(-)-linalool, showed different attractiveness depending on the mixing ratios of the two components in potato fields in South Korea. The best ratio for H. parallela attraction was 2.5:1 of LIME and (R)-(-)-linalool. The attraction ability of pheromone traps bated with 2.5:1 ratio did not drop up to 14m, but significantly decreased at 21m from H parallela release point. Korean population of H. parallela showed almost same periodical activity to sex pheromone with that reported in Japan, which showing a circabidian periodicity of 48h cycle.