• Molecules and Cells
• /
• 제23권3호
• /
• pp.379-390
• /
• 2007

We sequenced and characterized the complete mitochondrial genome of the Japanese fish tapeworm D. nihonkaiense. The genome is a circular-DNA molecule of 13607 bp (one nucleotide shorter than that of D. latum mtDNA) containing 12 protein-coding genes (lacking atp8), 22 tRNA genes and two rRNA genes. Gene order and genome content are identical to those of the other cestodes reported thus far, including its congener D. latum. The only exception is Hymenolepis diminuta in which the positions of trnS2 and trnL1 are switched. We tested a PCR-based molecular assay designed to rapidly and accurately differentiate between D. nihonkaiense and D. latum using species-specific primers based on a comparison of their mtDNA sequences. We found the PCR-based system to be very reliable and specific, and suggest that PCR-based identification methods using mtDNA sequences could contribute to the study of the epidemiology and larval ecology of Diphyllobothrium species.

• Parasites, Hosts and Diseases
• /
• 제54권4호
• /
• pp.509-518
• /
• 2016

Parasites are recorded from the red squirrel, Sciurus vulgaris, from Cheongju, the Republic of Korea. A total of 5 road-killed squirrels were thoroughly examined for internal and external parasites from November 2011 to May 2014. Total 4 parasite species, including 1 tapeworm and 3 ectoparasite species were recovered. They were morphologically identified as Catenotaenia dendritica (Cestoda: Catenotaeniidae), Hirstionyssus sciurinus, Leptotrombidium pallidum, and Ceratophyllus (Monopsyllus) indages. Among them, C. dendritica and H. sciurinus are recorded for the first time in the Korean parasite fauna. In addition, the possibility that the red squirrel could act as a reservoir host for a zoonotic disease like tsutsugamushi disease with L. pallidum as its vector has been raised.

• 한국동물위생학회지
• /
• 제14권1호
• /
• pp.79-86
• /
• 1991

This report was undertaken to examine the correlationship between internal parasite infection and amount of milk production before and after vermicidal administration on F. hepatica and gastrointestinal nematoda infected cattle. The obtained results were as follows ; 1. Out of total number(358), the ratios of parasitic infected catle were 43.5%(156). Among them milking cattl. and Korean native cattle were 52.7% and 31.6%, respectively. 2. In this examination, 10 kinds of parasites were detected. Among them F. hepatica was encountered most frquently and also Buxtonella, Paramphistoma, Coccidim, Cestoda, and Trichur were encountered in order. 3. The average increased amount of milk production after vermicidal administration on F. hepatica and gastrointestinal nematoda infected cattle was 1.1Kg per day.

• Parasites, Hosts and Diseases
• /
• 제30권2호
• /
• pp.147-150
• /
• 1992

한국에서의 유선조충(Mesocestcides lineatus)에 의한 두번째 인체기생례를 보고하였다. 참깨 같은 이물질을 대변 내에 배출하고 수개월 동안 간헐적인 복통을 호소한 45세 남자를 치료한 후 회수한 충체는 모두 32마리 이었다. 카민 염색한 성숙편절 및 수태편절에서 관찬되는 부자중(paruterine organ)과 충란의 특징적인 형태로 본 종을 동정할 수 있었다. 감염자는 양계장에 근무하면서 닭의 내장을 많이 생식하였다고 하였다.

• Parasites, Hosts and Diseases
• /
• 제44권3호
• /
• pp.233-237
• /
• 2006

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

• Parasites, Hosts and Diseases
• /
• 제33권3호
• /
• pp.225-230
• /
• 1995

저자등은 1994년 5월 경남 마산시에 거주하는 41세 남자 환자에게서 자연배출된 의엽 조충류의 충체 일부를 고래복식문조충(Diplogonoporus balaenopterae)으로 동정하였고 우리 나라의 인체 감염 제1례로 보고하는 바이다. 환자는 평소 각종 해산어류의 생식을 즐기고 담수어로는 잉어만을 생식한 경험이 있다. 검사실 소견상 경미한 호산구증다증(6%)과 혈청 IgE의 심한 증가(10,182 IU/ml). 초음파상에서 알콜성 지방간 외에는 특이한 소견을 발견할 수 없었다. Niclosamide 2 g을 경구투여하여 구충하였으나 두절을 발견하지 못하였다.

• Parasites, Hosts and Diseases
• /
• 제58권6호
• /
• pp.653-660
• /
• 2020

Spirometra tapeworms (Cestoda: Diphyllobothriidae) collected from carnivorous mammals in Tanzania were identified by the DNA sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and internal transcribed spacer 1 (ITS1), and by morphological characteristics. A total of 15 adult worms were collected from stool samples and carcasses of Panthera leo, Panthera pardus, and Crocuta crocuta in the Serengeti and Selous ecosystems of Tanzania. Three Spirometra species: S. theileri, S. ranarum and S. erinaceieuropaei were identified based on morphological features. Partial cox1 sequences (400 bp) of 10 specimens were revealed. Eight specimens showed 99.5% similarity with Spirometra theileri (MK955901), 1 specimen showed 99.5% similarity with the Korean S. erinaceieuropaei and 1 specimen had 99.5% similarity with Myanmar S. ranarum. Sequence homology estimates for the ITS1 region of S. theileri were 89.8% with S. erinaceieuropaei, 82.5% with S. decipiens, and 78.3% with S. ranarum; and 94.4% homology was observed between S. decipiens and S. ranarum. Phylogenetic analyses were performed with 4 species of Spirometra and 2 species of Dibothriocephalus (=Diphyllobothrium). By both ML and BI methods, cox1 and ITS1 gave well supported, congruent trees topology of S. erinaceieuropaei and S. theileri with S. decipiens and S. ranarum forming a clade. The Dibothriocephalus species were sisters of each other and collectively forming successive outgroups. Our findings confirmed that 3 Spirometra species (S. theileri, S. ranarum, and S. erinaceieuropaei) are distributed in the Serengeti and Selous ecosystems of Tanzania.

• Parasites, Hosts and Diseases
• /
• 제54권2호
• /
• pp.221-224
• /
• 2016

A visceral helminth of the squid, Todarodes pacificus, is reported from the East Sea, the Republic of Korea. Total 39 squid samples were purchased from a fish market in Jumunjin-eup, Gangneung-si (City) from August 2014 to July 2015 and were examined for helminth parasites with naked eyes and under a stereomicroscope after opening the abdominal cavity with a pair of scissors. Whitish larval worms were mainly found in the stomach and abdominal cavity of the squid. They were detected in 25 (64.1%) out of 39 squids examined, and the infection density was 7 larvae per infected squid. Spatula-shaped larvae were $8.2{\times}2.0mm$ in average size, round to slightly flattened anteriorly, with round hatching posteriorly, and had characteristic 4 tentacles with numerous hooklets in the scolex. The larvae were identified as the plerocercoid stage of Nybelinia surmenicola by their morphological features. This finding represents a new host record and the first report of N. surmenicola infection in T. pacificus squids from the east coast of Korea.

• Parasites, Hosts and Diseases
• /
• 제43권3호
• /
• pp.93-100
• /
• 2005

The present study was performed to investigate the infection status with helminth in a group of feral cats in Korea. More than 29 helminth species including adults or eggs were detected in visceral and fecal samples of the examined cats. Among these were a host of nematodes, including toxocarids, Ancylostoma sp. and the larva of Anisakis simplex; trematodes, including Clonorchis sinensis, Paragonimus westermani, Eurytrema pancreaticum, Pharyngostomum cordatum, Metagonimus spp., Heterophyes nocens, Pygidiopsis summa, Heterophyopsis continua, Stictodora fuscata, Stictodora lari, Acanthotrema felis, Stellantchasmus falcatus, Centrocestus armatus, Procerovum varium, Cryptocotyle sp., Echinostoma revolutum, Echinostoma hortense, Echinochasmus japonicus, Stephanoprora sp., Plagiorchis muris, Neodiplostomum sp. and diplostomulum. We also detected a variety of cestodes, including Spirometra erinacei, Taenia taeniaeformis and unidentified species of tapeworm. We also found examples of the acanthocephalan, Bolbosoma sp. In our assessment of the stools, we detected at least 12 species of helminth eggs. These findings confirmed that feral cats in Korea are infected with a variety of helminth parasite species. Furthermore, among the helminths detected, E. pancreaticum, S. fuscata, S. lari, A. felis, S. falcatus, C. armatus, P. varium, Cryptocotyle sp., E. revolutum, E. japonicus, Stephanoprora sp., P. muris, Neodiplostomum sp. and Bolbosoma sp. represent helminth fauna which have not been reported previously in feral cats in the Republic of Korea.

• 농촌의학ㆍ지역보건
• /
• 제15권2호
• /
• pp.89-96
• /
• 1990

The results of infection state of anisakid larvae and parasitic helminths in salmon and sea-trout which caught from Taep'o port. Kang-won do were as follows. 1) From twelve specimens of sea-trout. 122 individuals of anisakid larvae were found and mean infection number of anisakid larvae per individual sea-trout was 10.17. 2) From total extracted anisakid larvae from sea-trout. 37 larvae(30.3%) were found in the muscle. This percentage was lower than that of salmon. but higher than that of other sea-fishes. 3) The taxonomic list of parasitic helminths which found in salmon and sea-trout was as follows. Class Trematoda Family Hemiuridae l. Brachyphallus crenatus(Rudolphi. 1802) 2. Lecithaster salmonis Yamaguti. 1934 Class Cestoda Family Phllobothriidae 3. Pelichnibothrium speciosum Monticelli, 1889-larval form Family Tentacuariidae 4. Tentacularia sp. - larval form 4) Among those 4 species of helminths. B.crenatus. L.salmonis, P.speciosum were the first recording species in Korea.