Parasites, Hosts and Diseases

  • 제58권6호
  • /
  • Pages.653-660
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  • 2020
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Morphological and Molecular Identification of Spirometra Tapeworms (Cestoda: Diphyllobothriidae) from Carnivorous Mammals in the Serengeti and Selous Ecosystems of Tanzania

  • Ndosi, Barakaeli Abdieli (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Park, Hansol (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Lee, Dongmin (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Choe, Seongjun (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Kang, Yeseul (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Nath, Tilak Chandra (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Bia, Mohammed Mebarek (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Eamudomkarn, Chatanun (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Jeon, Hyeong-Kyu (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine) ;
  • Eom, Keeseon S. (Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University, School of Medicine)
  • 투고 : 2019.12.09
  • 심사 : 2020.10.25
  • 발행 : 2020.12.31
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초록

Spirometra tapeworms (Cestoda: Diphyllobothriidae) collected from carnivorous mammals in Tanzania were identified by the DNA sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and internal transcribed spacer 1 (ITS1), and by morphological characteristics. A total of 15 adult worms were collected from stool samples and carcasses of Panthera leo, Panthera pardus, and Crocuta crocuta in the Serengeti and Selous ecosystems of Tanzania. Three Spirometra species: S. theileri, S. ranarum and S. erinaceieuropaei were identified based on morphological features. Partial cox1 sequences (400 bp) of 10 specimens were revealed. Eight specimens showed 99.5% similarity with Spirometra theileri (MK955901), 1 specimen showed 99.5% similarity with the Korean S. erinaceieuropaei and 1 specimen had 99.5% similarity with Myanmar S. ranarum. Sequence homology estimates for the ITS1 region of S. theileri were 89.8% with S. erinaceieuropaei, 82.5% with S. decipiens, and 78.3% with S. ranarum; and 94.4% homology was observed between S. decipiens and S. ranarum. Phylogenetic analyses were performed with 4 species of Spirometra and 2 species of Dibothriocephalus (=Diphyllobothrium). By both ML and BI methods, cox1 and ITS1 gave well supported, congruent trees topology of S. erinaceieuropaei and S. theileri with S. decipiens and S. ranarum forming a clade. The Dibothriocephalus species were sisters of each other and collectively forming successive outgroups. Our findings confirmed that 3 Spirometra species (S. theileri, S. ranarum, and S. erinaceieuropaei) are distributed in the Serengeti and Selous ecosystems of Tanzania.

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참고문헌

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