• Journal of Physiology & Pathology in Korean Medicine
• /
• v.31 no.5
• /
• pp.264-269
• /
• 2017

Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin-induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ${\leq}300mg/dL$. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.

• Horticultural Science & Technology
• /
• v.28 no.3
• /
• pp.522-527
• /
• 2010

Onion has been reported to contain various organosulfur compounds which have antibiotic and anticarcinogenic properties and flavonoid like quercetin which is a valuable natural source of antioxidants. Carbohydrates in onion constitute about 80% of dry matter, and the major non-structural carbohydrate of onion bulb is fructo-oligosaccharides, well known as fructan, followed by glucose, fructose, and sucrose. The sugar concentration is associated with dormancy and storage life of onion, occurring as decrease in glucose, fructose and fructan, particularly towards the end of storage. Forced air pre-drying for 15-20 days at room temperature is an essential procedure to reduce freezing injury and sprouting, then onion bulbs can be stored at $0^{\circ}C$ for 6 months to control sprouting and decay. Bacterial soft rot caused by $Erwinia$ and $Pseudomonas$ is the main postharvest disease when the bulbs are infected with the bacteria and stored at room temperature. Browning in sliced onion is due to oxidation of phenolic compounds by polyphenol oxidase and it can be inhibited by citric acid treatment, packing with nitrogen gas, and polyethylene film.

• Horticultural Science & Technology
• /
• v.31 no.1
• /
• pp.128-132
• /
• 2013

A new pink gerbera (Gerbera hybrida Hort.) cultivar 'Party Time' was developed from a cross between 'Hope' and 'Sunnyeo', followed by seedling and line selections at the Flower Research Institute of Gyeongnam Agricultural Research and Extension Services (GARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2005 to 2007. 'Party Time' has semi-double type large flowers in a good harmony with pink (RHS, 65-C) ray floret and brown center. It has a good, stable flower shape and strong peduncles, and its vase life was 12.7 days. The average flower yield of 'Party Time' was about 47.3 stems per plant a year in greenhouse yield trails carried out 2005 to 2007. 'Party Time' (Grant No. 2691) was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009.

• Journal of Life Science
• /
• v.15 no.6 s.73
• /
• pp.851-856
• /
• 2005

Although valuable microbes have been isolated from the soil for the various productions of useful components, the microbes which can be cultivated in the laboratory are only $0.1-1\%$ of all microbes. To solve this problem, the study has recently been tried for making the valuable components from the environment by directly separating unculturable micrbial DNA in the soil. But it is known that humic acid originated from the soil interrupts various restriction enzymes and molecular biological process. Thus, in order to prevent these problems, this study modified the method separated soil DNA with phenol, CTAB and PEG. In order to compare the degree of purity for each DNA and the molecular biological application process, $A_{260}/A_{280}$ ratio, restriction enzymes, and PCR were performed. In case of DNA by the modified method, total yield of DNA was lower but $A_{260}/A_{280}$ ratio was higher than the previously reported methods. It was confirmed that the degree of purity is improved by the modified method. But it was not cut off by all kinds of tested restriction enzymes because of the operation of a very small amount of interrupting substances. When PCR was operated with each diluted DNA in different concentrations and GAPDH primer, the DNA by the modified method could be processed for PCR in the concentration of 100 times higher than by the previously reported separation method. Therefore, this experiment can find out the possibility of utilization for the unknown substances by effectively removing the harmful materials including humic acid and help establishing metagenomic DNA library from the soil DNA having the high degree of purity.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.595-598
• /
• 2009

A new yellow gerbera (Gerbera hybrida Hort.) cultivar "Golden Time" was developed from a cross between '99-ga-2' and '99-na-7-6', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2004 to 2007. "Golden Time" has semi-double type large flowers in a good harmony with yellow (RHS, 17-B) ray floret and brown center. It has good, stable flower shape and strong peduncles, and its vase life was 12.3 days. The average flower yield of "Golden Time" was about 49.5 stems per plant/year in greenhouse trails during 2004 and 2007. This cultivar was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009. Year-round production of this cultivar is possible in the greenhouse condition throughout the Korean climate.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.2
• /
• pp.103-109
• /
• 2011

This study was conducted to develop mass production method by proper cutting time, and the concentrations of plant growth regulators of Spiraea spp. Plants of hard wood was cut on March 3th, the rooting rate in S. salicifolia and S. cantoniensis was 11.3% comparatively higher than that about 5% in S. trichocarpa, S. miyabei, S. japonica, S. thunbergii and S. fritschiana. The rooting of control was 6.0%, however the $125{\sim}1,000mg{\cdot}L^{-1}$ IBA or NAA for 3 hour soaking was above 15.0% in the S. salicifolia. Therefore soaking in $125mg{\cdot}L^{-1}$ of IBA or NAA solution was recommended before cutting. The proper cutting time for soft wood from May 7 to September 17 in S. trichocarpa, S. cantoniensis, S. prunifolia, and S. thunbergii was September 17 when the soft stem was changed hard and the temperate was proper for rooting, high and low, $25{\pm}3^{\circ}C$ and $18{\pm}3^{\circ}C$. The rooting of September 17 was above 70% in S. salicifolia and S. fritschiana. However, the rooting from middle of May to early August was low below 20% in all the genus of Spiraea.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.3
• /
• pp.172-176
• /
• 2011

A new spray chrysanthemum (Dendranthema grandiflorum) cultivar, 'Magic Yellow' was developed from a cross between 'Artist pink' and 'S03-47' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2003 to 2008. Its characteristics were investigated three times from 2006 to 2008 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Magic Yellow' was October 18th, and year-round production was possible by day length treatment. This cultivar has single-type flowers in good harmony with bi-color ray floret and green center. Its flower was 6.0 cm in diameter, and it had 11.4 flowers per stem in autumn. The days to flowering under the short day treatment is about 45 days in spring season, and 'Magic Yellow' showed the vase life of 22.9 days in autumn. This cultivar was registered for the protection in 2008.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.3
• /
• pp.181-186
• /
• 2011

A new spray chrysanthemum cultivar 'Yellow Candy' was released by National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA), in 2008. The cross was made in 2003 between 'Restone' and 'Lollipop'. Trials were conducted from 2006 to 2008 for the evaluation and selection of this cultivar, including shading cultures in summer and retarding cultures in spring. The natural flowering time of 'Yellow Candy' is late October, but year-round flowering is possible by photoperiodic control. It has pompon flower type with yellow petals and yellowish red flower center. The growth of plant is very vigorous. The diameter of flower is 4.3 cm. Number of flowers per stem and petals per flower are 8 and 184, respectively. Days to flowering under the short day treatment is about 58.5 and its vase life is 18.5 days in autumn season. 'Yellow Candy' was applied as No. 2009-177 on Feb. 18, 2009 for variety protection and the plant variety protection rights have been registered as No. 3247 on August 3, 2010 at the Korea Seed and Variety Service.

• Korean Journal of Breeding Science
• /
• v.40 no.3
• /
• pp.340-343
• /
• 2008

A new pale yellow gerbera(Gerbera hybrida Hort.) cultivar 'Honey Cream' was developed from a cross between 'Princessa' and 'Picasso', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) in 2006. Three times evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Honey Cream' has semi-double type large flowers in good harmony with pale yellow(RHS, 11-D) ray floret and green center. It has good, stable flower shape and strong peduncle, and its vase life was 12.3 days. The average flower yield of 'Honey Cream' was about 48.5 stems per plant/year in greenhouse trails during 2003 and 2006. This cultivar was registered to the Korea Seed and Variety Service(KSVS) for commercialization in 2007. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.

• FLOWER RESEARCH JOURNAL
• /
• v.17 no.3
• /
• pp.201-204
• /
• 2009

A new orange mini gerbera (Gerbera hybrida Hort.) 'Yetty' was developed from a cross 'Haiti' and 'Nova Zembra' followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2001 to 2006. Three times evaluations were conducted from 2004 for the detailed characteristics of the new cultivar, 'Yetty' has single type mini flower in good harmony with orange (RHS 25-A) ray floret and browen center. It has good stable flower shape and strong peduncle, and its vase life was 14.8 days. The average yield of 'Yerty' was 98.5 flowers per plant a year in greenhouse yield trials carried out from 2004 to 2006. This cultivar registered for commercialization in 2007. 'Yerty' would be cultured under greenhouse condition in Korea.