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http://dx.doi.org/10.15188/kjopp.2017.10.31.5.264

Protective Effects of Prunella Vulgaris on Glomerular Injury in Streptozotocin-Induced Diabetic Rats  

Yoon, Jung Joo (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Park, Ji Hun (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Jeong, Da Hye (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Han, Byung Hyuk (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Choi, Eun Sik (Hanbang Body-fluid Research Center, Wonkwang University)
Lee, Yun Jung (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Kang, Dae Gill (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Lee, Ho Sub (Department of Phygiology, College of Korean Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.31, no.5, 2017 , pp. 264-269 More about this Journal
Abstract
Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin-induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ${\leq}300mg/dL$. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.
Keywords
Prunella vulgaris; Streptozotocin; Diabetic; Glomerular injury; Nephrin;
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