• Structural Engineering and Mechanics
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• 제24권3호
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• pp.355-374
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• 2006

Thin walled steel bridge-piers/columns are vulnerable to damage, when subjected to earthquake excitations. Local buckling, global buckling or interaction between local and global buckling usually is the cause of this damage, which results in significant strength reduction of the member. In this study new innovative design concepts, "thin-walled corrugated steel columns" and "thin-walled cellular steel columns" are presented, which allow the column to undergo large plastic deformations without significant strength reduction; hence dissipate energy under cyclic loading. It is shown that, compared with the conventional designs, circular and stiffened box sections, these new innovative concepts might results in cost-effective designs, with improved buckling and ductility properties. Using a finite element model, that takes the non-linear material properties into consideration, it is shown that the corrugations will act like longitudinal stiffeners that are supporting each other, thus improving the buckling behavior and allowing for reduction of the overall wall thickness of the column.

• 한국정보통신학회논문지
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• 제14권5호
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• pp.1201-1208
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• 2010

본 논문에서는 두 개의 선형 MLCA(Maximum Length Cellular Automata)을 이용한 영상 암호화 방법을 제안한다. 암호화 방법은 먼저 8 비트 초기 값을 임의로 설정한다. 그 다음, 설정된 초기 값을 이용하여 행과 열을 단계적으로 변화시켜 고품질의 PN(pseudo noise) 수열을 생성한다. 생성된 수열을 이용하여 기저영상을 생성한다. 마지막으로 기저영상을 원 영상과 XOR 연산함으로서 암호화 수준이 높은 결과 영상을 얻는다. 히스토그램 및 안정성 분석을 통하여 제안한 방법이 높은 암호화 수준의 성질을 가졌음을 검증한다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2009년도 추계학술대회
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• pp.953-955
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• 2009

본 논문에서는 두 개의 선형 MLCA(Maximum Length Cellular Automata)을 이용한 영상 암호화 방법을 제안한다. 암호화 방법은 먼저 8 비트 초기 값을 임의로 설정한다. 그 다음, 설정된 초기 값을 이용하여 행과 열을 단계적으로 변화시켜 고품질의 PN(pseudo noise) 수열을 생성한다. 생성된 수열을 이용하여 기저영상을 생성한다. 마지막으로 기저영상을 원 영상과 XOR 연산함으로서 암호화 수준이 높은 결과 영상을 얻는다. 히스토그램 및 안정성 분석을 통하여 제안한 방법이 높은 암호화 수준의 성질을 가졌음을 검증한다.

• 한국전자통신학회논문지
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• 제17권2호
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• pp.357-364
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• 2022

의사난수 생성기(PRNG)는 많은 양의 난수가 필요할 때 사용되는 프로그램이다. 대칭 키 암호시스템에서 대칭 키를 생성, 공개 키 암호나 디지털 서명에서 공개 키 쌍의 생성, 일회용 패드로 패딩에 사용되는 열을 생성하는 데 사용한다. 다양한 과학 분야에서 비선형 동역학계를 구체적으로 표현하는데 유용한 셀룰라 오토마타(CA)는 이산적이고 추상적인 계산 시스템으로 하드웨어 구현이 가능하여 암호시스템에서 키를 생성하는 PRNG로 응용되고 있다. 본 논문에서는 이웃 셀의 반경을 2로 증가한 5-이웃 CA를 이용하여 비선형 수열을 효과적으로 생성할 수 있는 프로그램 가능한 5-이웃 CA기반의 PRNG를 합성하는 알고리즘을 제안한다.

• 생명과학회지
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• 제12권4호
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• pp.490-495
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• 2002

병원성 미생물인 Vibrio fluvialis로부터 hemolysin을 분리정제 하였다. 즉, hemolysin의 정제는 균 배양액을 황산암모늄, ion exchange chromatography를 행하였으며 SDS-PAGE를 통해 그 단백질의 크기가 약 79kDa임을 알게되었다. 정제된 hemolysin (VFH)는 35$^{\circ}C$에서 최적활성을 나타내었고, 4$0^{\circ}C$에서는 그 활성이 감소되었다. RTG-2 어류유래 세포주에 hemolysin (VFH)의 처리로 세포독성을 측정한 결과 50$\mu\textrm{g}$의 VFH가 약 80%의 cell line을 사멸시켰다. 또한 현미경을 통한 관찰에서도 세포의 형태변화를 관찰할 수 있었다.

• Toxicological Research
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• 제8권2호
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• pp.343-357
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• 1992

Tumor necrosis factor-${\alpha}$(TNF), a polypeptide hormone secreted primarily by activated macrophages, was originally identified on the basis of its ability to cause hemorrhagic necrosis and tumor regression in vivo. Subsequently, TNF has been shown to be an important component of the host responses to infection and cancer and may mediate the wasting syndrome known as cachexia. These systemic actions of TNF are reflected in its diverse effects on target cells in vitro. TNF initiates its diverse cellular actions by binding to specific cell surface receptors. Although TNF receptors have been identified on most of animal cells, regulation of these receptors and the mechanisms which transduce TNF receptor binding into cellular responses are not well understood. Therefore, in the present study, the mechanisms how TNF receptors are being regulated and how TNF receptor binding is being transduced into cellular responses were investigated in rat liver plasma membranes (PM) and ME-180 human cervical carcinoma cell lines. $^{125}I$-TNF bound to high ($K_d=1.51{\pm}0.35nM$)affinity receptors in rat liver PM. Solubilization of PM with 1% Triton X-100 increased both high affinity (from $0.33{\pm}0.04\;to\;1.67{\pm}0.05$ pmoles/mg protein) and low affinity (from $1.92{\pm}0.16\;to\;7.57{\pm}0.50$ pmoles/mg protein) TNF binding without affecting the affinities for TNF, suggesting the presence of a large latent pool of TNF receptors. Affinity labeling of receptors whether from PM or solubilized PM resulted in cross-linking of $^{125}I$-TNF into $M_r$ 130 kDa, 90 kDa and 66kDa complexes. Thus, the properties of the latent TNF receptors were similar to those initially accessible to TNF. To determine if exposure of latent receptors is regulated by TNF, $^{125}I$-TNF binding to control and TNF-pretreated membranes were assayed. Specific binding was increased by pretreatment with TNF (P<0.05), demonstrating that hepatic PM contains latent TNF receptors whose exposure is promoted by TNF. Homologous up-regulation of TNF receptors may, in part, be responsible for sustained hepatic responsiveness during chronic exposure to TNF. As a next step, the post-receptor events induced by TNF were examined. Although the signal transduction pathways for TNF have not been delineated clearly, the actions of many other hormones are mediated by the reversible phosphorylation of specific enzymes or target proteins. The present study demonstrated that TNF induces phosphorylation of 28 kDa protein (p28). Two dimensional soidum dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) resolved the 28kDa phosphoprotein into two isoforms having pIs of 6.2 and 6.1. The pIs and relative molecular weight of p28 were consistent with those of a previously characterized mRNA cap binding protein. mRNA cap binding proteins are a class of translation initiation factors that recognize the 7-methylguanosine cap structure found on the 5' end of eukaryotic mRNAs. In vitro, these proteins are defined by their specific elution from affinity columns composed of 7-methylguanosine 5'-triphosphate($m^7$GTP)-Sepharose. Affinity purification of mRNA cap binding proteins from control and TNF treated ME-180 cells proved that TNF rapidly stimulates phosphorylation of an mRNA cap binding protein. Phosphorylation occurred in several cell types that are important in vitro models of TNF action. The mRNA cap binding protein phosphorylated in response to TNF treatment was purifice, sequenced, and identified as the proto-oncogene product eukaryotic initiation factor-4E(eIF-4E). These data show that phosphorylation of a key component of the cellular translational machinery is a common early event in the diverse cellular actions of TNF.

• IMMUNE NETWORK
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• 제8권1호
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• pp.7-12
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• 2008

Background: Members belonging to the interferon-lambda (IFN-${\lambda}$) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-${\lambda}$ biology, such as endocytosis of IFN-${\lambda}$, we produced monoclonal antibodies (Abs) against human IFN-${\lambda}$ and examined their usefulness. Methods: We purified recombinant human IFN-${\lambda}$1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-${\lambda}$1-immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-${\lambda}$1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-${\lambda}$ and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity. Results: Four hybridoma clones secreting Abs specific to IFN-${\lambda}$1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-${\lambda}$1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-${\lambda}$1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-${\lambda}$ complex on HepG2 cells. Conclusion: Monoclonal Abs against IFN-${\lambda}$1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-${\lambda}$.

• Applied Microscopy
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• 제18권2호
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• pp.153-166
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• 1988

The fine structure of the salivary glands of the pine moth, Dendrolimus spectabilis Butler, at the last larval period is observed using light and electron microscopes. The moths have single paired tubular salivary glands which openings are connected to the oral cavity through the upper jaw. By the external morphology and its functions, the glands are subdivided into three regions which are anterior reabsorptive region, middle storage region and posterior secretory region. Along the inner canal of the salivary gland two columns of the large glandular cells are connected each other and oriented to ring-like forms. By this cellular orientation, the glands have long and large tubular structure. From anterior to posterior region large nuclei of the glands are ramified like twigs of the tree, and in the cytoplasm of the cell numerous mitochondria and vacuoles are seen. Moreover, basal plasma membranes of the gland cells are heavily infolded. The anterior region of the glands keeps several characteristics related to the reabsorption of the material from the inner cavity to the glandular cells whereas, main salivary material is synthesized and secreted through the long and convoluted posterior region. The apical plasma membranes of the cells are the most heavily invaginated at the posterior regoin, but trachea and tracheoles are distributed only at the middle and posterior regions. In the cytoplasm of the middle region Golgi complexes appeared at the vicinity of the vesicles, and at the posterior region of the salivary glands multivesicular bodies are also observed.

• Steel and Composite Structures
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• 제23권2호
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• pp.195-204
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• 2017

The effects of plaster on the behavior of single-story single-bay masonry-infilled steel frames under in-plane base accelerations have been experimentally investigated by a shake-table. Tested structures were made in a 1/3 scale, with realistic material properties and construction methods. Steel frames with high and low flexural rigidity of beams and columns were considered. Each type of frame was tested with three variants of masonry: (i) non-plastered masonry; (ii) masonry infill with conventional plaster on both sides; and (iii) masonry infill with a polyvinyl chloride (PVC) net reinforced plaster on both sides. Masonry bricks were made of lightweight cellular concrete. Each frame was firstly successively exposed to horizontal base accelerations of an artificial accelerogram, and afterwards, to horizontal base accelerations of a real earthquake. Characteristic displacements, strains and cracks in the masonry were established for each applied excitation. It has been concluded that plaster strengthens the infill and prevents damages in it, which results in more favorable behavior and increased bearing capacity of plastered masonry-infilled frames compared to non-plastered masonry-infilled frames. The load-bearing contribution of the adopted PVC net in the plaster was not noticeable for the tested specimens, probably due to relative small cross section area of fibers in the net. Behavior of masonry-infilled steel frames significantly depends on frame stiffness. Strong frames have smaller displacements than weak frames, which reduces deformations and damages of an infill.

• 한국전자통신학회논문지
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• 제14권2호
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• pp.439-446
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• 2019

본 논문에서는 효율적으로 의료 영상을 보호하기 위하여 C-MLCA와 1차원 CAT를 이용한 암호화 방법을 제안한다. 먼저, Wolfram 규칙으로 상태 전이 행렬을 생성한 후 최대 길이의 수열을 만든다. 다음으로 여원 벡터를 곱하여 복잡한 수열로 변환한다. 그리고 두 수열을 행과 열로 곱하여 원 의료 영상 크기의 기저 영상을 생성한 후 XOR 연산을 한다. 마지막으로 게이트 웨이값을 설정하여 만들어진 1차원 CAT 기저함수와 CAT 변형 계수가 적용된 영상을 연산하면, 최종적으로 암호화된 영상을 얻을 수 있다. 암호화된 영상은 원 의료 영상과 비교하기 위해 히스토그램과 PSNR을 사용하여 평가한다. 또한 NPCR과 키 공간 분석을 통하여 제안한 방법의 안정성을 검증한다.