• Title/Summary/Keyword: cell-recycling fermentation

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Optimization of Food Waste Fermentation for Probiotic Feed Production with Yeast Kluyveromyces marxianus

  • Lee, Ki-Young;Yu, Sung-Jin;Yu, Seung-Yeng
    • Proceedings of the Korean Institute of Resources Recycling Conference
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    • 2001.05b
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    • pp.121-125
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    • 2001
  • For the probiotic feed production, aerobic liquid fermentation of pulverized food wastes was attempted with a yeast Kluyveromyces marxianus. After grinding finely, optimal fermentation conditions of the substrate was investigated by shaking culture. The most active growth of the yeast was shown at solid content of 10%. The proper addition of urea(0.5g/l), o-phosphate(0.4g/l), molasses(4g/l), and yeast extract (1g/1) increased cell growth rate and viable cell count. For optimizing, the nutrients were all added to substrate and fermentation was carried in 2 litre jar fermenter. For the stimulation of hydrolyzing enzyme excretion, mixed culture with Aspersillus oryzae was also conducted. In 12 hours of fermentation, viable cell count of the yeast Kluyveromyces marxianus amounted to the number of 1.4 $\times$10$^{10}$ /1 in the culture medium.

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Production of Organic Acids from Food By-Products - Mass Production of Organic Acids by Continuous Flow Ceil Recycling Fermentation - (식품부산물로부터 유기산의 대량생산공정에 관한 연구 - 세포재순환식 연속발효를 이용한 유기산의 대량 생산 -)

  • Ju Yun-Sang;Jin Sun-Ja;Hwang Pil-Gi;Choi Chul-Ho;Lee Eui-Sang
    • KSBB Journal
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    • v.19 no.6 s.89
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    • pp.484-488
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    • 2004
  • Fermentation studies were conducted in batch and continuous flow cell-recycle reactors with food by-products as substrates. The genus Propionibacterium acidipropionici ATCC 4965 was utilized in the production of organic acids. Good performance was achieved in the batch fermentation using hydrol as a carbon source and corn steep liquor (CSL) as nitrogen and vitamin sources. Product yields and productivity based on maximum values were 0.80 g total acids/g glucose and 0.26 g total acids/L/h, respectively, when $3\%$, (w/v) of hydrol and $2.5\%$, (w/v) of CSL were utilized. Continuous fermentation with cell-recycling system using the optimum amounts of substrates resulted in dramatic increase in cell concentration (X) and maximum productivity (P). Compared to the batch fermentation, X and P were increased by as much as 21 and 13 times, respectively, at the dilution ratio of $0.2\;hr^{-1}$, indicating that cell recycling fermentation of food by-products provides valuable means for the mass production of organic acids as well as utilizing cell mass as good nutrient resources.

Continuous Ethanol Fermentation Using Membrane Cell Recycle Fermentor (Membrane Cell Recycle Fermentor에 의한 에탄올 연속 발효)

  • 김태석;이석훈;손석민;권윤중;변유량
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.419-427
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    • 1991
  • Ethanol fermentation of glucose by a strain of Saccharomyces cereuisiae was studied in membrane recycle bioreactor, where the fermentation vessel was coupled with cross flow hollow fiber membrane. The cell recycle system controlled backflushing with fresh medium was proven to be effective in alleviating membrane fouling and allowing long term operation of high-cell continuous fermentation. Using 100 g/l initial glucose concentration, the maximum productivity of about 9 5 g/$l \cdot h$ has been achieved at dilution rate 2.5 $h^{-1}$ and bleed stream ratio 0.05 with the corresponding ethanol concentration of 35g/l and glucose conversion of 100%. Increasing the glucose concentration to 200 g/$l \cdot h$ resulted in an increase in ethanol concentration to 48 g/l and productivity to 120 g1l.h. Substrate conversion, however, was only 69%. This productivity was the highest value in the study, and about 38 fold more than that of batch culture and 17 fold more that of single stage continuous culture without cell recycling. No further increase in the productivity was obtained when the glucose concentration was increased reased to 300g/l.

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Aerobic Liquid Fermentation of Residual Food Waste by Thermophilic Bacteria (고온세균을 이용한 남은 음식물의 호기적 액상발효)

  • Ryu, Seung-Yong;Park, Myoung-Ju;Kim, So-Young;Lee, Ki-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.3
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    • pp.126-131
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    • 2002
  • For the probiotic feed production from residual food waste, aerobic liquid fermentation was conducted by thermophilic bacteria. 11 Strains of bacteria were isolated from several soil sources and residual food waste. Screening was carried by shaking incubator for the separation of thermophilic strain at $55^{\circ}C$. The isolated strains were tested for enzyme activities such as ${\alpha}$-amylase and protease. 6 Bacterial strains were chosen and were adapted by repeated fermentation processes in food waste substrate. The viable cell count of them at final fermentation stages were shown as $3-7{\times}10^9/ml$ in 2L-jar fermenter. Among them B3, B6 showed higher enzyme activity. By the mixed fermentation of B3, B6 and Bacillus stearothermophilus, the highest viable cell count reached to $1.4{\times}10^{10}/ml$ in 8 hours.

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Microalgae Removal and Energy Production by Combined Electro-flotation and Anaerobic Hydrogen Fermentation Processes (전기부상과 혐기성 수소 발효 공정의 결합을 통한 미세조류 제거 및 에너지 생산)

  • Lee, Chae-Young;Na, Dong-Chae;Choi, Jae-Min;Kang, Doo-Sun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.3
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    • pp.83-88
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    • 2012
  • The algal bloom, resulting from eutrophication, has caused serious water quality problems in river and lake. Therefore, it has to be removed by any means including physicochemical or biological treatment for preserving water quality. This study was conducted to investigate the microalgae removal and energy production using combined electro-flotation and anaerobic hydrogen fermentation processes. The result showed that algae removal efficiency based on chlorophyll a removal increased with the current. At a current of 0.6A, the maximum microalgae removal efficiency of 95.9% was achieved. The treatability of anaerobic hydrogen fermentation was investigated to recover energy from microalgae removed by electro-flotation. The ultimate hydrogen yields of algae before and after ultrasonic pretreatment were 17.3 and 61.1 ml $H_2/g$ dcw(dry cell weight), respectively. The ultrasonic pretreatment of algae led to 3.4-fold higher $H_2$ production due to the increase of hydrolysis rate.

Aerobic Liquid Fermentation of Food Wastes by Using Yeast (효모에 의한 남은 음식물의 호기성 액상발효)

  • Lee, Ki-Young;Yu, Sung Jin;Yu, Seung Yeung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.8 no.4
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    • pp.147-152
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    • 2000
  • For the probiotic feed production from residual food waste by using the yeast Kluyveromyces marxianus, aerobic liquid fermentation was attempted at $35^{\circ}C$. After grinding finely, optimal fermentation conditions of the substrate was investigated in shaking incubator. By controlling water content yeast growth was studied at each different solid content of 5, 10 and 15% respectively. The most active growth of the yeast was shown at 10%. For the stimulation of the cell growth, mixed culture with Aspersillus oryzae was conducted in a 2 litre-jar fermenter. As the results, the yeast growth rate was increased, but the maximum viable cell count amounted was slightly higher as $3.5{\times}10^9/ml$ than single culture.

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Influence of Agitation Speed on Cell Growth in the Aerobic Yeast Fermentation of Pulverized Liquid Food Wastes for Probiotic Feed Production (남은 음식물로 호기적 액상효모발효를 이용한 생균사료를 생산할 때 생균수에 대한 교반 속도의 영향)

  • Yu, Sung-Jin;Yu, Seung-Yeung;Lee, Ki-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.4
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    • pp.99-104
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    • 2001
  • The influence of agitation speed on the yeast growth was investigated in the production of probiotic feed from pulverized liquified food wastes by aerobic fermentation. A yeast Kluyvermyces marxianus was selected through a preliminary screening. The yeast was cultured by 2liter jar fermenter. in 10% solid(w/v) substrate of liquified food waste at $35^{\circ}C$ with each different agitation speed of 500, 900 and 1200 rpm. For the acceleration of enzyme excretion mixed culture with Aspergillus oryzae was also attempted and the results were compared to those of single culture. As results the viable cell number was increased by increasing agitation speed. But it showed highest value in 900rpm and then decreased in 1200rpm. The mixed culture increased amylase activity and growth rate, but did not seem to enhance the highest viable cell count in the final fermentation stage.

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Influence of Nutrient Addition in the Liquid Yeast Fermentation of Pulverized Food Wastes (남은 음식물의 습식효모배양에서 영양물질첨가가 효모증식에 미치는 영향)

  • Lee, Ki-Young;Yu, Sung-Jin;Chae, Hee-Jung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.1
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    • pp.49-55
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    • 2001
  • For the production of probiotic feed enriched with viable yeasts, aerobic liquid culture of Kluyveromyces marxianus was attempted in pulverized residual food wastes. After the preliminary shaking culture result, the liquid food wastes was added with urea($0.5g/{\ell}$), o-phosphate($0.4g/{\ell}$ ), molasses($4g/{\ell}$), and yeast extract($1g/{\ell}$), and the fermentation was carried out in 2-litre jar fermenter. In 12 hours of aerobic mixed culture with Aspersillus oryzae, viable cell count of the yeast reached to the number of $1.4{\times}10^{10}/{\ell}$ in the cultured medium.

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The Manufacture of Inoculum for Fermented Pig Feed Production from Food Wastes (음식물류폐기물의 돼지 발효사료화를 위한 종모배양액 제조)

  • Lee, Kyung-Seok;Hong, Seung-Yoon;Kim, Young-Jun;Lee, Ki-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.15 no.2
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    • pp.98-108
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    • 2007
  • In order to use food wastes for the source of fermented feed for pigs, this study was aimed to produce better culture inoculum by the aeration and addition of pig' s blood meal as sub nutrient. For the preparation of inoculum as bacterial strain, Lactobacillus brevis isolated from pig intestine, and a yeast Saccharomyces cerevisiae from strawberries were used. Molasses and whey were used as main ingredients for the culture solution as well as yeast extract and other ingredients as sub nutrients. As the experimental result, aeration showed a positive effect to enhance viable cell count or retarding death phase. Although sub nutrient yeast extracts were replaced with pig's blood meal, fermentation characteristics were almost similar to that of yeast extract. When the inoculum was stored at room temperature, L. brevis and S. cerevisiae maintained the viable cell concentration of approximately 8 log cfu/mL for 1 week. 2 Days after the culture solution was mixed with food waste, the number of unwanted bacteria had rapidly increased, but E.coli was not detected for 5 days.

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Production of Fermented Feed from Food Wastes by Using Inoculation (종균 첨가에 의한 음식물 찌꺼기의 발효 사료화)

  • Suh, Eun-Hee;Song, Eun-Seung;Han, Uok;Lee, Sung-Taek;Yang, Jae-Kyung;Lee, Ki-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.5 no.1
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    • pp.1-13
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    • 1997
  • The fermentative conversion of food wastes into feed was investigated by seeding of mixed inoculum YM (Youngjin Environmental co.), and thermotolerant yeast Kl. marxianus. For 6 days' fermentaion, the fermentation method of 2 days' aerobic followed by 4 days' anaerobic was better for the production of organic acids and increasing total microbial population than 6 days' continuous aerobic or anaerobic fermentation. By seeding YM, the total microbial count increased about 100 times of the control group. In addition, Kl. marxianus seeding together with YM increased total viable cell count, but did not increase yeast count significantly.

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