• Title/Summary/Keyword: cell wall thickness

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Effects of Abscisic Acid on the Epidermal Structure and Ontogeny of Stomata in Orostachys malacophyllus Leaves (둥근바위솔(Orostachys malacophyllus) 잎의 표피구조와 기공발생에 미치는 Abscisic Acid의 영향)

  • 김진성
    • Journal of Plant Biology
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    • v.30 no.1
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    • pp.21-30
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    • 1987
  • The effects of abscisic acid(ABA) spraying for 12 weeks on the stomatal types and frequencies of O. malacophyllus leaves were summarized as follows. ABA inhibited the growth of O. malacophyllus. The prominent effect of ABA on the epidermal structure was the promotion of senescence such as thickness of cell walls, smooth sinuosity of cell walls, and large size of epidermal cells. The stomatal frequency was decreased to 23% by 10$\mu\textrm{g}$ ml-1 ABA and to 48% by 100$\mu\textrm{g}$ml-1, and also the stomatal size was more or less smaller than that of control. The developing secondary stomatal mother cell was not found in both 10 and 100$\mu\textrm{g}$ml-1ABA, but the arrested secondary stomatal mother cell was rarely found in 10$\mu\textrm{g}$ ml-1 ABA. The formation of normal stomatal types such as helico-eumesogenous and aniso-eumesogenous was found in both 10 and 100 $\mu\textrm{g}$ ml-1 ABA asin well as control. Also nine abnormal stomatal types were found, and the frequencies were promoted to 6% by 10 $\mu\textrm{g}$ ml-1 ABA and to 17% by 100 $\mu\textrm{g}$ ml-1 ABA. Among these abnomal stomata, four types such as aborted stomata, single-aborted guard cells, arrested stomata, and modified stomatal complexes were found in control as well as in 10 and 100 $\mu\textrm{g}$ ml-1 ABA, but five types such as wrenched stomata, unequal stomata, wavy guard cells, guard cells overlapped by guard cells, and dissolved cell wall stomata were found in both 10 and 100 $\mu\textrm{g}$ ml-1ABA. The modified stomata complexes were abnormal stomatal types which were newly found and also were varied in types.

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Histochemical and Cytochemical Studies on the Secondary Xylem Formation during Grafting Process of Pinus thunbergii (접목 과정에서 형성된 신생 2차목부의 조직학적, 세포화학적 연구)

  • Kim, Jong Sik;Lee, Kwang Ho;Kim, Yoon Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.6
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    • pp.1-11
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    • 2006
  • Grafted tissues were investigated using various microscopic techniques. Pinus thunbergii was used as stock and scion and autografted by cleft graft method. Histochemically, grafting processes can be proceeded by four stages: 1) formation of necrotic layer, 2) proliferation of callus, 3) development of neo-cambium from callus, and 4) restoration of new vascular xylem. Necrotic la yer composed of pectin and lignin was gradually degraded during grafting process and disappeared when new union was formed between stock and scion. A large number of starch and lipid bodies in the cytoplasm were also gradually degraded during grafting process and disappeared at the grafting interface. Nucleus and plasmodesmata were not changed. Bubble-like callus was generated from all living parenchyma cells and from the callus. The tracheary elements differentiated from the callus had either reticulate or pit-like thickenings in the secondary walls with bordered pits. Secondary cell wall thickening occurred toward filing to the void parts between reticulated secondary wall. Tracheids formed in the secondary xylem were short with irregular wall thickness. New secondary xylem cells with swirled shapes, which developed in graft union were oriented horizontally and obliquely to axis of the stem.

Effects of Foliar Spray of Calcium Hydroxide on Shoot Growth and Fruit Quality in 'Daewol' Peach (Prunus persica Batsch) (수산화칼슘 엽면살포가 '대월' 복숭아의 신초 생육 및 과실 품질에 미치는 영향)

  • Park, Ji-Young;Son, In-Chang;Kim, Dae-Il
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.102-108
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    • 2010
  • The effects of foliar spray of calcium hydroxide ($Ca(OH)_2$) during fruit growth period was investigated by changes of the shoot growth and fruit quality in 'Daewol' peach (Prunus persica). Since foliar spray of $Ca(OH)_2$ 1, 2, 5, and 10 $g{\cdot}L^{-1}$ at 7 day-intervals after fruit thinning, shoot growth was remarkably decreased compared with control group. An average SPAD value of fifth leaf from proximal part of the shoot was higher as 42.1 specific color difference sensor value (SCDSV) of $Ca(OH)_2$ foliar spraying treatments than 40.9 SCDSV of control group. Photosynthesis rates were also significantly increased by treating $Ca(OH)_2$ of higher concentration. Among fruit characteristics affecting quality, fruit weight was increased depending on concentration of $Ca(OH)_2$ treatment. The soluble solids content was lowest in control group (8.78 $^{\circ}Brix$) compare with higher concentrations of $Ca(OH)_2$ foliar spraying treatment in each 9.17, 9.22, 9.71, 10.58 $^{\circ}Brix$. The acidity and anthocyanin contents were no significant differences among treatment, but firmness of pericarp and flesh of fruits was significantly increased by $Ca(OH)_2$ foliar spray treatment. As a results of morphological observation of leaf, thickness of palisade parenchyma was thinner in control group (63.5 ${\mu}m$) than those of each 86.5, 87.5, 93.6, 107.4 ${\mu}m$ in $Ca(OH)_2$ foliar spraying treatment. Higher $Ca(OH)_2$ foliar spray also increased the thickness of cell wall of epidermis and hypodermis in 'Daewol' fruit.

A Study on Broad-Band Design of Electromagnetic Wave Absorber in Ferrite Cylinder Insertion Type (페라이트 기둥 삽입형 전파흡수체의 광대역 설계에 관한 연구)

  • 이창우;김동일
    • Journal of the Korean Institute of Navigation
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    • v.23 no.1
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    • pp.23-28
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    • 1999
  • With a rapid progress in electronic industry we enjoy various conveniences of life. As many kinds of information equipments are supplied even to most of individuals as to be called an information society, we are exchanging much information with one another surprisingly. Consequently the occupation density of microwave frequency band is highly increased, and electromagnetic environment is getting more seriously bad. It often gives fatal blow to even human life and thus becomes serious social problems. Electromagnetic wave absorbers for anechoic chamber are needed to broaden the effective frequency bandwidth, reduce the thickness, and decrease the weight. There are various absorbers proposed for the above conditions, but they could not decisively solve it the alone requirements. The Electromagnetic wave absorber made by a conventional ferrite tile has, for example, broadened the useful frequency bandwidth by the way of forming air layer(practically use urethane foam, etc.) on the ferrite tile. Therefore, an air layer is formed between a reflection plate and a sintered Ni-Zn ferrite tile of 7 mm in thickness, which has reflectivity less than -20 dB from 30 MHz to 400 MHz in bandwidth. Accordingly, in this paper, a broadened electromagnetic wave absorber are designed, which has the reflection characteristics less than -20 dB from 30 MHz to 6,000 MHz in the bandwidth. Then we achieved the goal by design the inserting square Ferrite Cylinders with the thickness less than 17.5 mm on existing grid type Ferrite absorber. The purpose of this research is on the development of very wide-band electromagnetic absorber for a universal anechoic chamber for measuring radiated electromagnetic wave or immunity of electronic equipments, GTEM-cell, wall material for preventing TV ghost, etc.

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Metabolic changes during adaptation to saline condition and stress memory of Arabidopsis cells

  • Chun, Hyun Jin;Park, Mi Suk;Lee, Su Hyeon;Jin, Byung-Jun;Cho, Hyun Min;Hong, Young-Shick;Kim, Min Chul
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.175-175
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    • 2017
  • To understand molecular mechanisms underlying adaptation of plant cells to saline stress and stress memory, we developed Arabidopsis callus suspension-cultured cells adapted to high salt. Adapted cells to high salt exhibited enhanced tolerance compared to control cells. Moreover, the salt tolerance of adapted cells was stably maintained even after the stress is relieved, indicating that the acquired salt tolerance of adapted cells was memorized. In order to characterize metabolic responses of plant cells during adaptation to high salt stress as well as stress memory, we compared metabolic profiles of salt-adapted and stress-memorized cells with control cells by using NMR spectroscopy. A principle component analysis showed clear metabolic discrimination among control, salt-adapted and stress-memorized cells. Compared with control cells, metabolites related to shikimate metabolism such as tyrosine, and flavonol glycosides, which are related to protective mechanism of plant against stresses were largely up-regulated in adapted cell lines. Moreover, coniferin, a precursor of lignin, was more abundant in salt-adapted cells than control cells. Cell morphology analysis using transmission electron microscopy indicated that cell wall thickness of salt-adapted cells was significantly induced compared to control cells. Consistently, salt adapted cells contained more lignin in their cell walls compared to control cells. The results provide new insight into mechanisms of plant adaptation to saline stress as well as stress memory in metabolic level.

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MORPHOMETRICS OF ALVEOLAR PROCESS AND ANATOMICAL STRUCTURES AROUND INFERIOR MAXILLARY SINUS FOR MAXILLARY IMPLANTATION (임플랜트 시술을 위한 치조돌기와 상악동 주변 구조물의 형태계측적 연구)

  • Park, Ju-Jin;Lee, Young-Soo;Paik, Doo-Jin;Park, Won-Hee;Yoo, Dong-Yeob
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.2
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    • pp.228-239
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    • 2007
  • Statement of problem: Following tooth loss, the edentulous alveolar process of maxilla is affected by irreversible reabsorption process, with progressive sinus pneumatization leads to leaving inadquate bone height for placement of endosseous implants. Grafting the floor of maxillary sinus by sinus lifting surgery and augmentation of autologous bone or alternative bone material is a method of attaining sufficient bone height for maxillary implants placement and has proven to be a highty successful. Purpose: This study was undertaken to clarify the morphometric characteristics of inferior maxillary sinus and alveolar process for installation of implants. Material and method: Nineteen skulls (37 sinuses, 10M / 9F) obtained from the collection of the department of anatomy and cell biology of Hanyang medical school were studied. The mean age of the deceased was 69.9 years (range 44 to 88 years). The distance between alveolar border and inferior sinus margin at each tooth, the height of alveolar process and the thickness of cortical bone of the outer and inner table of alveolar process and the inferior wall of maxillary sinus were measured. Results and Conclusion: 1. The septum of inferior maxillary sinus were observe 28 sides (76.%) and located at the third molar (52.6%) and the second molar (26.3%). The deepest points of inferior border of maxillary sinus were located the first or second molar. The distance between alveolar margin and the deepest point of inferior maxillary sinus is $9.7{\pm}4.9mm$. 2. The length of the outer table of alveolar process were $4.9\sim28.2mm$ and the shortest point was between the first and the second molors. The thickness of them were $0.9\sim3.2mm$. The length of the inner table of alveolar process were $7.4\sim25.8mm$ and the shortest point was between the first and the second molars. The thickness of the were $0.9\sim4.6mm$. The results of this study are useful anatomical data for installing of maxillary implants.

Surgical Treatment of Squamous cell Carcinomas Arising in Scalp Burn Wounds - Two Case Reports - (화상 후 두피에 생긴 편평 상피세포 종양에 대한 증례 보고 - 증례보고 -)

  • Kim, Kang San;Hwang, Hyung Sik;Kwon, Heum Dai;Moon, Seung Myung;Oh, Suk Jun;Choi, Sun Kil
    • Journal of Trauma and Injury
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    • v.20 no.1
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    • pp.52-56
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    • 2007
  • Marjolin's ulcer is a rare and often-aggressive cutaneous malignancy that arises in previously traumatized or chronically inflamed skin, particularly after burns. We experienced two cases after burns. Case I involved a forty eight year-old man who had suffered from a flame burn at the parietal scalp area, where had been initially described three years earlier as a full-thickness wound including the pericranium. The man consulted us for a persistent ulcerative and infected wound on the burned lesion during the last 24 months, which turned out on the contrast-enhanced computed tomography (CT) and magnetic resonance imaging (MRI) to be the squamous cell carcinoma with involving the skull and the dura mater. Although the posterior auricular lymph node was enlarged on the ipsilateral side, recent positron emission tomography (PET) CT did not show any metastatic lesion. It was impossible for us to resect the intracranial involvement of the tumor radically, and the postoperative PET CT still showed a focal fluorodeoxyglucose (FDG) uptake around the wall of the superior sagittal sinus. We think that an aggressive combined approach is essential for treatment in early stages for a high success rate, before the intracranial structures are involved because there is no consensus on the treatment for advanced disease, and the results are generally poor. Case 1 also did not involve a radical resection because of the intracranial invasion to the wall of superior sagittal sinus and the possibility of damage to the major cortical veins. He received adjuvant radiotherapy and must be followed periodically. Case 2 involved an eighty six year-old women who suffered from a painful scalp ulcer lesion after flame burns three years earlier. Unlike case 1, neither tumor infiltration into the dura nor lymph node enlargement was observed on the contrast-enhanced computed tomography (CT), magnetic resonance imaging (MRI), or positron emission tomography (PET) CT. We did a radical resection of the tumor, including the involved bone, and a cranioplasty with bone cement.

Visualization of the Gastric Calcification due to Cancer on Tc-99m DPD and Abdominal CT Images (Tc-99m DPD 골스캔과 복부 CT 영상에서 보이는 위암의 석회화)

  • Jeong, Young-Jin;Kang, Do-Young
    • The Korean Journal of Nuclear Medicine
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    • v.38 no.5
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    • pp.344-346
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    • 2004
  • A 69-year-old woman was presented with progressed dysphagia, gastric soreness and weight loss during 2 months. She was performed abdomen x-ray, EGDS and abdomen CT. Abdomen x-ray demonstrated punctuate calcification on LUQ. EGDS showed an ulceroinfiltrative mass with bleeding on cardia to antrum of stomach. And CT showed diffuse gastric wall thickness with multiple calcifications. Biopsy of the stomach and esophagus during EGDS examination revealed an adenocarcinoma, with signet ring cell type, infiltrating the wall of the stomach and the distal esophagus. Then acne scan was performed a few days later. It revealed intense uptake in LUQ, corresponding to the calcium containing neoplasm seen on the abdomen x-ray, EGDS and abdomen CT. And there was no evidence of any metastatic lesion and thyroid uptake on the bone scan. There are many reports about accumulation of the tracer in extraosseous lesion, but only a few literatures were reported about gastric calcification in stomach cancer. More over, no reports showed CT images. We are performed many diagnostic examinations and found well correlation between them. The reason of gastric calcification is considered with calcium deposition within extracellular space due to hemorrhage or necrosis. Other possibility offered to explain gastric calcification have been increased blood flow and/or increased neovascularity with capillary leaks of tracer, and specific enzymatic (phosphatases) receptor binding of tracer. So, it was happened ion exchange between intracellular calcium and phosphate groups of tracer.

Inhibitory Effects of Angelica gigas Nakai on Ulcerative Colitis in DSS-induced ICR Mice (당귀 추출물의 궤양성 대장염 억제 효과에 대한 연구)

  • Nam, Hae In;Baik, Tae Hyun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.30 no.6
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    • pp.439-446
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    • 2016
  • The purpose of this study was to examine the effects of Angelica gigas Nakai on ulcerative colitis. Mice were divided into 5 groups: Control group, DSS group, 5-ASA group, 50mg/kg Angelica gigas Nakai group, 100mg/kg Angelica gigas Nakai group. Four groups, excluding the control group, were fed a 5% solution of dextran sulfate sodium(DSS) in water for 7days to induce ulcerative colitis. Each water extract was administrated orally for 7 days in 5-ASA group, 50mg/kg Angelica gigas Nakai group and 100mg/kg Angelica gigas Nakai group. 5 groups were evaluated by weight, length of intestine, weight of spleen, disease activity index(DAI), amount of cytokine IL-6 production, thickness of bowel wall and degree of inflammatory cell infiltration and intestinal tissue damage. Comparing to DSS group, 100 mg/kg Angelica gigas Nakai group showed significant suppressive effect of weight loss until 4th day of experiment while 50 mg/kg Angelica gigas Nakai group showed no significant effect of suppression. Decrease of intestinal length, enlargement of spleen, intestinal tissue damage and thickening of bowel wall were significantly suppressed in both 50 mg/kg and 100mg/kg Angelica gigas Nakai group. Also disease activity and cytokine IL-6 production were inhibited significantly. Based on this result, Angelica gigas Nakai seemed to have anti-inflammatory effect and also seemed to suppress histological changes and aggravation of ulcerative colitis.

Synthesis of Hollow Mesoporous Carbon Nitride Spheres Using Polystyrene Spheres as Template (폴리스티렌 구형입자를 주형으로 이용한 할로우 메조포러스 질화탄소 구형입자의 합성)

  • Park, Sung Soo;Ha, Chang-Sik
    • Journal of Adhesion and Interface
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    • v.15 no.2
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    • pp.63-68
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    • 2014
  • Hollow mesoporous carbon nitride material with sphere shape was synthesized using polystyrene sphere as template and cyanamide as nitrogen and carbon atom sources via thermal treatment process. The process of the silica removal is not necessary because silica as template is not in use for the synthesis of hollow mesoporous carbon nitride material and any solvents are also not in use. The size of polystyrene spheres was about 170 nm. Hollow diameter and wall thickness were 82 nm and 13 nm, respectively, in hollow mesoporous carbon nitride sphere. Surface area, mesopore size and pore volume of hollow mesoporous carbon nitride material was $188m^2g^{-1}$, 3.8 nm and $0.35cm^3g^{-1}$, respectively. The wall in hollow sphere has graphitic structure. Hollow mesoporous carbon nitride material has potential applications in the area of fuel cell, catalysis, photocatalysis, electroemmision device, etc.