• Title/Summary/Keyword: cell wall components

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Study on the Displacement of Crib Wall System (Crib Wall System 변위해석에 관한 연구)

  • Kim, Doo-Jun;Lee, Song
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.6 no.3
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    • pp.201-209
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    • 2002
  • Crib wall system is one of segmental crib type wall. Crib walls are constructed from separate members with no bonds between them other than frictional. The wall units are divide into two main types termed headers and stretchers. The headers run from the front to the back of the wall, perpendicular to the wall face. The cells are created by forming a grid by stacking individual wall components known as headers and stretchers. The body of wall consists of a system of open cell which are filled with a granular material. The design of crib retaining wall is usually based on conventional design methods derived from Rankine and Coulomb theory so that is able to resist the thrust of soil behind it, because it may be assumed that the wall acts as a rigid body. However, deformation characteristics of crib walls cannot be assumed as monolithic. They consist of individual members which have been stacked to creat a three dimensional grid. Therefore, the segmental grid allows relative movement between the individual member within the wall. The three dimensional flexible grid leads to stress distribution by interaction behavior between soil and crib wall. Therefore, in this study, in order to analysis the trends of deflection of crib wall system, new numerical models based on the results of Brandl's full scale test are introduced for design concept.

An Investigation of Swirling Flow in a Cylindrical Tube

  • Chang, Tae-Hyun;Kim, Hee-Young
    • Journal of Mechanical Science and Technology
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    • v.15 no.12
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    • pp.1892-1899
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    • 2001
  • An experimental study was performed for measuring velocity and turbulence intensity in a circular tube for Re= 10,000, 15,000 and 20,000, with swirl and without swirling flow. The velocity fields were measured using PIV techniques and swirl motion was produced by a tangential inlet condition. Some preliminary measurements indicated that over the first 4 diameter, two regions of flow reversal were set up (the so called 2-cell structure). At the highest Reynolds numbers, the maximum values of the measured axial velocity components had moved toward the test tube wall and produce more flow reversal at the conter of the tube. As the Reynolds number increased, the turbulence intensity of swilling flow at the tube inlet also increased.

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Design of a Bridge Transported ServoManipulator System for a Radioactive Environment

  • Park, B.S.;Jin, J.H.;Ahn, S.H.;Song, T.G.;Kim, D.G.;Yoon, J.S.
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2514-2518
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    • 2003
  • The KAERI Spent Fuel Remote Technology Development (SFRTD) Department is developing the remote maintenance and repair equipment, which is used in a hot cell in an intense radiation field, as part of a project to develop the Advanced spent fuel Conditioning Process (ACP). Although several mechanical master-slave manipulators (MSMs) is mounted on the hot cell wall, their reach will be limited and cannot access areas for all the ACP equipment maintenance. A Bridge Transported ServoManipulator (BTSM) has been designed to overcome the limitation of access areas that is a drawback of MSMs for the ACP equipment maintenance. The BTSM system consists of four components: a transporter with telescoping tubeset, a slave manipulator, a master manipulator, and a remote control system. The BTSM system has been designed by Solid Edge that is a 3D computer-aided design (CAD) software, except for the remote control system. The master manipulator and the slave manipulator are kinematically similar in design, except for the handle and the tong, respectively. The manipulators have 6 degrees of freedom (DOF) plus the jaws motion. The transporter has traveling, traverse, and hoisting motion to position the slave manipulator.

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A Bridge Transported Bilateral Force-Reflecting Servo-Manipulator for Maintenance of Nuclear Pyroprocessing Equipment

  • Park, B.S.;Jin, J.H.;Ko, B.S.;Lee, J.K.;Yoon, J.S.
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.2226-2230
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    • 2005
  • The Advanced Spent Fuel Conditioning Process (ACP), which is a pre-disposal treatment process for spent fuel is being developed at the Korea Atomic Energy Research Institute (KAERI). The ACP equipment is operated in an intense radiation field as well as in a high temperature. Thus, the equipment is designed in consideration of the remote handling and maintenance. This paper describes a Bridge Transported Bilateral Force-Reflecting Servo-Manipulator (BTSM) system, which is being developed to overcome the limitation of access that is a drawback of the mechanical Master-Slave Manipulators (MSMs), which are mounted on the ACP hot cell wall for the operation and the maintenance of the ACP equipment. The BTSM system was manufactured and temporally installed at the mockup to test its performance. The manufactured BTSM system will be installed at the ACP hot cell on June 2005 after the accomplishment of the performance test. The BTSM system consists of four components: a transporter with a telescoping tubeset, a slave manipulator, a master manipulator, and a remote control system. This system will highly increase the volume of coverage for the operation and maintenance of the ACP equipment.

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Abdominal Endometriosis Diagnosed by Fine Needle Aspiration Cytology -A Report of Two Cases- (세침흡인 세포검사로 진단된 복벽의 자궁내막증 -2예 보고-)

  • Chung, Myoung-Ja;Jeong, Yeon-Jun;Hwang, Ho-Myong;Jang, Kyu-Yoon;Moon, Woo-Sung;Kang, Myoung-Jae;Lee, Dong-Geun
    • The Korean Journal of Cytopathology
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    • v.15 no.1
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    • pp.70-73
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    • 2004
  • The incidence of endometriosis in post-operative abdominal scars is rare. We describe two cases of abdominal endometriosis diagnosed by fine needle aspiration (FNA). Both patients presented with subcutaneous masses at previous cesarean section scars with cyclic symptoms of pain. The cytologic smears were cellular and comprised two distinct cell populations consisting of epithelial and stromal components. An epithelial component consisted of flat sheets of polygonal cells and the second stromal component showed crowded clusters of spindle cells or isolated single cells. Hemosiderin-laden macrophages were found in the background. FNA offers a safe and effective tool for diagnosis of abdominal wall endometriosis.

Convenient Assay of O2- Generated on Potato Tuber Tissue Slices Treated with Fungal Elicitor by Electron Spin Resonance - No Secondary Oxidative Burst Induction by H2O2 Treatment

  • Park, Hae-Jun;Doke, Noriyuki
    • The Plant Pathology Journal
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    • v.21 no.3
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    • pp.283-287
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    • 2005
  • Since the discovery of generation of $O_2^-$ in plant, many evidence for the oxidative burst (OXB) has been accumulated in various combinations of plant and pathogen or elicitor systems. $O_2^-$ generating system responsible for the OXB was coupled with oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) in microsomal fraction isolated from sliced aged potato tuber slices which were treated by hyphal wall components elicitor from Phytophthora infestans (HWC). We developed new assay method for quantitative measurement of oxygen radical $O_2^-$ by using electron spin resonance (ESR) analysis during elicitor­induced OXB on the surface of plant tissues. The ESR analysis using an $O_2^-$ trapper, Tiron (1,2-dihydroxy-3,5­benzenedisulfonic acid), provided a convenient assay for detecting only $O_2^-$ during elicitor-induced OXB producing various active oxygen species (AOS) on plant tissue surface. Tiron was oxidized to Tiron semiquinon radical by $O_2^-$. Quantity of the radical signal was measured by specific spectra on ESR spectroscopy. The level of $O_2^-$ was high in from surface of potato tuber tissue treated with hyphal cell wall elicitor (HWC) from Phytophthora infestans. There was no secondary OXB induction by $H_2O_2$ treatment in plant.

Pathogenicity of Bacillus Strains to Cotton Seedlings and Their Effects on Some Biochemical Components of the Infected Seedlings

  • Aly, Aly A.;El-Mahdy, Omima M.;Habeb, Marian M.;Elhakem, Abeer;Asran, Amal A.;Youssef, Maryan M.;Mohamed, Heba I.;Hanafy, Rania S.
    • The Plant Pathology Journal
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    • v.38 no.2
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    • pp.90-101
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    • 2022
  • Pathogenicity of eight Bacillus strains to seedlings of four cotton cultivars was evaluated under greenhouse conditions. Each of the tested cultivars was individually treated with powdered inoculum of each bacterial strain. Untreated seeds were planted as control treatments in autoclaved soil. Effects of the tested strains on levels and activities of some biochemical components of the infected seedlings were also assayed. The biochemical components included total soluble sugars, total soluble proteins, total free amino acids, peroxidase, polyphenol oxidase, phenols, and lipid peroxidation. ANOVA showed that Bacillus strain (B) was a very highly significant source of variation in damping-off and dry weight. Cotton cultivar (V) was a nonsignificant source of variation in damping-off while it was a significant source of variation in dry weight. B × V interaction was a significant source of variation in damping-off and a nonsignificant source of variation in dry weight. Bacillus strain was the most important source of variation as it accounted for 59.36 and 64.99% of the explained (model) variation in damping-off and dry weight, respectively. The lack of significant correlation between levels and activities of the assayed biochemical components and incidence of damping-off clearly demonstrated that these biochemical components were not involved in the pathogenicity of the tested strains. Therefore, it was hypothesized that the pathogenicity of the tested strains could be due to the effect of cell wall degrading enzymes of pathogenic toxins. Based on the results of the present study, Bacillus strains should be considered in studying the etiology of cotton seedling damping-off.

Ultrasturctural Study on Nectar Secretion from Extrafloral Nectary of Prunus yedoensis Matsumura (왕벚나무 화외밀선의 당액 분비에 관한 미세구조적 연구)

  • 정병갑
    • Journal of Plant Biology
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    • v.35 no.2
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    • pp.143-153
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    • 1992
  • Nectar secretion from extrafloral nectary cells of Prunus yedoensis was examined by light and electron microscopy. Nectaries were composed of two or three layers of secretory cells and one layer of subsectretory cells. Vascular bundles in the petioles were connected to those of the subsectretory cell layer. Secretory cells had a number of mitochondria with poorly developed cristae. Plastids had little thylakoids and small vesicles, about 0.2 to 0.3 mm in diameter; however, no plastids had starch grains. Calcium oxalate crystals and plasmodesmata were frequently observed in the subsectretory and secretory cells, respectively. And nectar substances were observed in phloem of petiole, subsectretory, and secretory cells of the secretory gland. These results suggested that the nectar moved by symplastic transport through the plasmodesmata. On the other hand, the nectar droplets were observed in the secretory cell walls. in the cuticular layer just beyond of the former, and on the outer surface of the cuticular layer: such observations indicated that a apoplastic movement was involved in the final step of the nectar secretion. Cellular components related to the nectar transport, such as plasma membrane, cell wall and cuticle were not destroyed but intact: it was interpreted as a eccrine secretion.retion.

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Effect of Medium Composition on Protopectinase Production from Bacillus subtilis EK11 (Bacillus subtilis EK11로부터 Protopectinase 생산을 위한 배지성분의 영향)

  • 이대희;박은경;문철환;하정욱;이승철;황용일
    • Microbiology and Biotechnology Letters
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    • v.27 no.5
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    • pp.378-384
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    • 1999
  • Protopectinase (PPases) are heterologous group of enzymes that degrade pectin from the insoluble protopection which is constituent of the middle lamella and primary cell wall of higher plants by restricted depolymerization. From the previous report[6], enzymatically separated plant cells, which are produced from plant tissues by PPases treatment, showed well-conserved cellular components with their rigid cell wall and this characteristic is applicable to preparation of novel food material. The purpose of this study is to investigate the effect of medium composition of PPase production from Bacillus subtilis EK11 which was selected as a PPase producer. Various carbon sources and concentrations on PPase production were studied and corn starch at 0.7% was the most effective for production of PPase. Among the nitrogen sources, yeast extract was the most effective for PPase production and the effect of (NH4)2SO4 was notable as inotganic nitrogen source. Inorganic compounds such as KH2PO4, K2HPO4, Na3-citrate.2H2O and MgSO4 were optimized for PPase production. PPase activity was inhibited by the adition of Ba2+ or Zn2+. The optimal medium for PPase production was devised: 0.7% corn starch, 0.3% yeast extract, 1.4% KH2PO4, 0.6% K2HPO4, 0.1% Na3-citrate.2H2O and 0.02% MgSO4. PPase production by using the optimum medium was carried out with shaking cultivation at 37$^{\circ}C$. The maximum PPase activity of 256unit/ml could be obtained after the cultivation for 48hrs. The activity was increased about 2.2timesthan the activity, 112 unit/ml, in basal medium.

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$\alpha$-1,3-glucanase를 생산하는 미생물의 분리, 동정 및 배양조건

  • 김명섭;신원철
    • Microbiology and Biotechnology Letters
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    • v.24 no.4
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    • pp.465-471
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    • 1996
  • A strain producing $\alpha$-1, 3-glucanase was isolated from soil samples. The strain has a grey aerial mycelium and showed brown color from the other side. The temperature and pH range for growth were from 10$\circ$C to 42$\circ$C and from 4 to 10, respectively. Arabinose, dextrin and mannose were utilized for growth. Analysis of cell wall components revealed that the strain was classified as type I. From the results, the strain was identified as Streptomyces sp. By using this strain, the maximum production of 0.65 units/ml for $\alpha$-1, 3-glucanase was achieved at 37$\circ$C for 48 hrs.

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