• Title/Summary/Keyword: cell wall components

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Structural Components of Cell Wall in Various Parts of Korean Ginseng(Panax ginseng C. A. Meyer) (고려인삼의 부위별 세포벽 구성성분)

  • 정영륜;박찬수
    • Journal of Ginseng Research
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    • v.7 no.2
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    • pp.169-171
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    • 1983
  • The structural components of cell wall in various parts of Korean ginseng were analysed. Pectin was abundant in the cortex and epidermis of root, and leaf. Hemicellulose in root was more than in other parts of ginseng. Cellulose content of stem and seed coat was much higher compared to other parts and lignin content was highest in seed coat.

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Changes in the Cell Wall Components, Enzyme Activities and Cell Structure of Peach during Maturation (복숭아의 품종과 숙도에 따른 세포벽 성분, 효소활성 및 조직의 변화)

  • 김미현;신승렬
    • Food Science and Preservation
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    • v.1 no.2
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    • pp.107-116
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    • 1994
  • This paper was carried out to Investigate changes in the activities of cell-degrading enzymes, cell wall components and cell structure of peach during maturation and storage for valuation of quality. The firmness of peach was decreased during maturation and storage, and was remarkably decreased in Daegubo than Yumyung. Polygalacturonase and $\beta$-galactosidase activities of peach were increased during maturation and storage, and were remarably increased in soft peach and in mature and soft peach, respectively. Contents of alcohol-insoluble substance, cell wall, and total and insoluble pectin of peach were decreased during maturation and storage, but cellulose and soluble pectin were increased. Intracellular space was enlarged during maturation and middle lamella was gradually degraded during maturation.

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Changes in the Cell Wall Components of Peach during Maturation and Storage (복숭아의 성숙 및 저장중의 세포벽 성분의 변화)

  • 김미현;신승렬;손미애;김광수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.4
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    • pp.372-376
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    • 1992
  • This study was carried out to investigate changes in the cell wall components of peach during maturation and storage. The hardness of peach was decreased from $24.1\;\pm\;2.2N\;to\;14.6\;\pm\;1.3N$ during maturation and storage. Contents of alcohol-insoluble substance, water-soluble material and cell wall were decreased. Total and insoluble pectic substances were decreased while water-soluble pectin was increased during maturation and storage. Contents of cellulose were increased. Galactose and arabinose contents were decreased during maturation and storage.

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Blood Component Change in Rat by Lipopolysaccharide and Cell Wall Protein-A from Vibrio vulnificus, E. coli, and S. typhimurium

  • Lee, Bong-Hun
    • Journal of Life Science
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    • v.10 no.2
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    • pp.9-11
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    • 2000
  • Lipopolysaccharide (LPS) and cell wall protein-A (CWP-A) were extracted from the cell wall of Vibrio vulnificus, Escherichia coli and Salmonella typhimurium. LPSs and CWP-As were injected into rat and the changes of the following blood components were examined. The change of the number of white blood cell (WBC), red blood cell (RCB), platelet (PLT), blood urea nitrogen (BUN) and blood glucose in rat blood and interferon (IFN) activity change by LPS and CWP-A were measured. WBC, RETI, PTT, and BUN were increased and RBC and blood glucose were increased slightly, but PLT was decreased.

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Cell Biological Studies on Growth and Dovelopment - Effect of polyamines on D-glucose-6-phoshate cyclohydrolase antivity in carrot cells- (생체 생장에 관한 세포 생물학적 연구 - 당근 세포의D-glucose-6-phosphate cyclohydrolase 활성에 미치는 polyamine의 영향 -)

  • 조영동
    • Journal of Plant Biology
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    • v.29 no.4
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    • pp.263-284
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    • 1986
  • Effects of putrescine, spermidine and spermine on the activity of D-glucose-6-phosphate cyclohydrolase in the Daucus carota L. protoplast cultured for 4 days and effects of polyamines on the incorporation of D-[u-14C]-glucose treated to protoplasts in culture-medium were investigated. The activity of D-glucose-6-phosphate cyclohydrolase was increased by polyamines and among them spermine was the most effective. Polyamiens increased protein synthesis and this due to the increasing effect of the polyamines on the synthesis of glycoprotein which is one of cell wall components. The synthesis of cell polysaccharides, such sa pectic substances, hemicelluloses and cellulose was increased by polyamines, which stimulated synthesis of pectin substances, and hemicellulose more greatly than that of cellulose, and spermidine was the most effective. In the light of the above results it seems that the polyamines increase cell wall regeneration by the stimulation of enzyme activities which synthesize cell wall components.

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In Vivo Immunopotentiating Effects of Cellular Components from Lactococcus lactis ssp. lactis

  • Kim Ji-Yeon;Lee Seong-Kyu;Jeong Do-Won;Hachimura Satoshi;Kaminogawa Shuichi;Lee Hyong-Joo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.5
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    • pp.786-790
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    • 2006
  • Cellular components of Lactococcus lactis ssp. lactis (heat-killed whole cells, cytoplasm, and cell walls) were tested for their in vivo immunopotentiating activity. Peritoneal macrophages from mice orally administered with heat-killed whole cells exhibited significantly greater phagocytic activity than the groups administered with cell-wall fraction or cytoplasm fraction. The cytotoxicity of natural-killer cells was the highest in the group administered with whole cells, and the production of cytokines ($IFN-\gamma$, IL-2, and IL-12) in spleen cells was significantly higher, when cellular components were injected, and it tended to be higher in the cell-wall and cytoplasm groups than in the whole-cell group. Interestingly, the cytokine production of Peyer's patch cells was high, when cytoplasm fractions were administered. These results demonstrate that whole cells and cytoplasm and cell-wall fractions of L. lactis ssp. lactis have immunopotentiating activities, which are related to the stimulation of Peyer's patches.

Characterization of an Apple Polygalacturonase-Inhibiting Protein (PGIP) That Specifically Inhibits an Endopolygalacturonase (PG) Purified from Apple Fruits Infected with Botryosphaeria dothidea

  • Lee Dong-Hoon;Bae Han-Hong;Kang In-Kyu;Byun Jae-Kyun;Kang Sang-Gu
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1192-1200
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    • 2006
  • An apple polygalacturonase-inhibiting protein (PGIP), which specifically inhibits endopolygalacturonase (PG, EC 3.2.1.15) from Botryosphaeria dothidea, was purified from Botryosphaeria dothidea-infected apple (Malus domestica cv. Fuji) fruits. The purified apple PGIP had a molecular mass of 40 kDa. The N-terminal amino acid sequence of the purified protein showed high homologies to those of PGIP from pear (100%), tomato (70%), and bean (65%). We also purified polygalacturonase (PG) from B. dothidea. The PG hydrolyzes pectic components of plant cell walls. When the extracted apple pectic cell wall material was treated with purified apple PGIP and B. dothidea PG, the amount of uronic acid released was lower than that treated with B. dothidea PG alone. This result demonstrates that PGIP functions specifically by inhibiting cell wall maceration of B. dothidea PG Furthermore, we characterized the de novo function of the PGIP against PG on the solubilization and depolymerization of polyuronides from cell wall of apple fruits inoculated with B. dothidea. This result demonstrated that the PGIP of plants exhibits one of the direct defense mechanisms against pathogen attack by inhibiting PGs that are released from pathogens for hydrolysis of cell wall components of plants.

Streptomyces sp. YJB-599가 생산하는 Genistein의 분리 및 정제

  • 함병권;배동훈;유주현
    • Microbiology and Biotechnology Letters
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    • v.24 no.3
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    • pp.311-315
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    • 1996
  • A cytotoxic material was produced by strain No. 5-99 which was isolated from soil. Analyzing the cell wall components, LL-diaminopimelic acid was identified. From the existance of glycine in the cell wall, this strain was identified to Streptomyces sp. which has cell wall chemotype I and peptidoglycan type A3 connected by glycine. So, we named this strain to Streptomyces sp. YJB-599. The Active material was purified through solvent extraction, silica gel column chromatography and crystallized to needle-shaped white -crystal. Analyzing the structure of this crytal by instrumental analysis and database, it was determined to genistein.

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Adsorption of $\textrm{Pb}_{2+}$ in the components of bacterial cell membrane

  • Kim, Mal-Nam
    • Journal of Microbiology
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    • v.33 no.4
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    • pp.278-282
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    • 1995
  • S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant $\textrm{Pb}_{2+}$ per unit dry weight of the three fractions tested. Adsorption behavior of $\textrm{Pb}_{2+}$ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more $\textrm{Pb}_{2+}$ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of $\textrm{Pb}_{2+}$ than total native cell membrane or cell membrane lipid.

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Micromorphological Characteristics of Frost Rings in the Secondary Xylem of Pinus radiata

  • Lee, Kwang Ho;Kim, Jong Sik;Singh, Adya P.;Kim, Yoon Soo
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.2
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    • pp.1-8
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    • 2007
  • Frost ring formed in the secondary xylem of Pinus radiata was examined using various microscopic techniques. Cell walls in a frost ring were poorly developed, lacking in the proportion of wall components. Formation of secondary cell wall was imperfect and thickness of secondary wall was varied. Cytochemical examinations provided the evidence that the synthesis of structural polysaccharides and lignin was inhibited, resulting in the malformation of secondary cell walls. Judging by the highly irregular nature of the cell wall, it appears that cellulosic/hemicellulosic framework was affected and the template for lignification by frost.