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In Vivo Immunopotentiating Effects of Cellular Components from Lactococcus lactis ssp. lactis  

Kim Ji-Yeon (Department of Food Standard Evaluation, Korea Food & Drug Administration)
Lee Seong-Kyu (Department of Applied Biological Chemistry, The University of Tokyo)
Jeong Do-Won (School of Agricultural Biotechnology, and Center of Agricultural Biomaterials, Seoul National University)
Hachimura Satoshi (Department of Applied Biological Chemistry, The University of Tokyo)
Kaminogawa Shuichi (Department of Food Science and Technology, College of Bioresource Science, Nihon University)
Lee Hyong-Joo (School of Agricultural Biotechnology, and Center of Agricultural Biomaterials, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.5, 2006 , pp. 786-790 More about this Journal
Abstract
Cellular components of Lactococcus lactis ssp. lactis (heat-killed whole cells, cytoplasm, and cell walls) were tested for their in vivo immunopotentiating activity. Peritoneal macrophages from mice orally administered with heat-killed whole cells exhibited significantly greater phagocytic activity than the groups administered with cell-wall fraction or cytoplasm fraction. The cytotoxicity of natural-killer cells was the highest in the group administered with whole cells, and the production of cytokines ($IFN-\gamma$, IL-2, and IL-12) in spleen cells was significantly higher, when cellular components were injected, and it tended to be higher in the cell-wall and cytoplasm groups than in the whole-cell group. Interestingly, the cytokine production of Peyer's patch cells was high, when cytoplasm fractions were administered. These results demonstrate that whole cells and cytoplasm and cell-wall fractions of L. lactis ssp. lactis have immunopotentiating activities, which are related to the stimulation of Peyer's patches.
Keywords
Cytokine; immunopotentiating activity; Lactococcus lactis ssp. lactis; NK cell activity; phagocytic activity;
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1 Bae, E.-A., N.-Y. Kim, M. J. Han, M.-K. Choo, and D.-H. Kim. 2003. Transformation of ginsenosides to compound K (IH-901) by lactic acid bacteria of human intestine. J. Microbiol. Biotechnol. 13: 9-14
2 Haller, D., S. Blum, C. Bode, W. Hammes, and E. J. Schiffrin. 2000. Activation of human peripheral blood mononuclear cells by nonpathogenic bacteria in vitro: Evidence of NK cells as primary targets. Infect. Immun. 68: 752-759   DOI
3 Hessle, C., L. A. Hanson, and A. E. Wold. 1999. Lactobacilli from human gastrointestinal mucosa are strong stimulators of IL-12 production. Clin. Exp. Immunol. 116: 276-282   DOI
4 Lee, H.-Y., J.-H. Park, S.-H. Seok, S.-A. Cho, M.-W. Baek, D.-J. Kim, Y.-H. Lee, and J.-H. Park. 2004. Dietary intake of various lactic acid bacteria suppresses type 2 helper T cell production in antigen-primed mice splenocyte. J. Microbiol. Biotechnol. 14: 167-170   DOI   ScienceOn
5 Perdigon, G., S. Alvarez, and H. A. P. de Ruiz. 1991. Immunoadjuvant activity of oral Lactobacillus casei: Influence of dose on the secretory immune response and protective capacity in intestinal infections. J. Dairy Res. 58: 485-496   DOI
6 Perdigon, G., M. E. N. de Macias, S. Alvarez, G. Oliver, and H. A. P. de Ruiz. 1986. Effect of orally administered lactobacilli on macrophage activation in mice. Infect. Immun. 53: 404-410
7 Perdigon, G., M. E. N. de Macias, S. Alvarez, G. Oliver, and H. A. P. De Ruiz. 1990. Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus. J. Dairy Sci. 57: 255-264
8 Gilliland, S. E. 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol. Rev. 7: 175-188   DOI
9 Cross, M. L., R. R. Mortensen, J. Kudsk, and H. S. Gill. 2002. Dietary intake of Lactobacillus rhamnosus HN001 enhances production of both Th1 and Th2 cytokines in antigen-primed mice. Med. Microbiol. Immunol. 191: 49-53   DOI
10 Yoshizawa, Y., J. Tsunehiro, K. Nomura, M. Itoh, F. Fukui, A. Ametani, and S. Kaminogawa. 1996. In vivo macrophagestimulation activity of the enzyme-degraded water-soluble polysaccharide fraction from a marine alga (Gracilaria verrucosa). Biosci. Biotechnol. Biochem. 60: 1667-1671   DOI   ScienceOn
11 Saito, H., H. Tomioka, and K. Nagashima. 1987. Protective and therapeutic efficacy of Lactobacillus casei against experimental murine infections due to Mycobacterium fortuitum complex. J. Gen. Microbiol. 133: 2843-2851
12 Murosaki, S., K. Muroyama, Y. Yamamoto, and Y. Yoshikai. 2000. Antitumor effect of heat-killed Lactobacillus plantarum L-137 through restoration of impaired interleukin-12 production in tumor-bearing mice. Cancer Immunol. Immunother. 49: 157-164   DOI
13 Kato, I., K. Tanaka, and T. Yokokura. 1999. Lactic acid bacterium potently induces the production of interleukin-12 and interferon-${\gamma}$ by mouse splenocytes. Int. J. Immunopharmacol. 21: 121-131   DOI   ScienceOn
14 De Simone, C., R. Vesely, R. Negri, S. B. Bianchi, S. Zanzoglu, A. Cilli, and L. Lucci. 1987. Enhancement of immune response of murine Peyer's patches by a diet supplemented with yogurt. Immunopharmacol. Immunotoxicol. 9: 87-100   DOI
15 Kelly, J. M., P. K. Darcy, J. L. Markby, D. I. Godfrey, K. Takeda, H. Yagita, and M. J. Smyth. 2002. Induction of tumor-specific T cell memory by NK cell-mediated tumor rejection. Nature Immunol. 3: 83-90   DOI   ScienceOn
16 Kim, H.-J., J.-H. Kim, J. H. Son, H.-J. Seo, S.-J. Park, N.-S. Paek, and S.-K. Kim. 2003. Characterization of bacteriocin produced by Lactobacillus bulgaricus. J. Microbiol. Biotechnol. 14: 503-508
17 Perdigon, G., M. E. Nader de Macias, S. Alvarez, M. Medici, G. Oliver, and H. A. A. Pesce de Ruiz. 1987. Enhancement of immune response in mice fed with Streptococcus thermophilus and Lactobacillus acidophilus. J. Dairy Sci. 70: 919-926   DOI   ScienceOn
18 Takagi, A., T. Matsuzaki, M. Sato, K. Nomoto, M. Morotomi, and T. Yokokura. 2001. Enhancement of natural killer cytotoxicity delayed murine carcinogenesis by a probiotic microorganism. Carcinogenesis 22: 599-605   DOI   ScienceOn
19 Yasutake, N., T. Matsuzaki, K. Kimura, S. Hashimoto, T. Yokokura, and Y. Yoshikai. 1999. The role of tumor necrosis factor (TNF)-${\alpha}$ in the antitumor effect of intrapleural injection of Lactobacillis casei strain Shirota in mice. Med. Microbiol. Immunol. 188: 9-14   DOI
20 Ouwehand, A. C., S. Salminen, and E. Isolauri. 2002. Probiotics: An overview of beneficial effects. Antonie van Leeuwenhoek 82: 279-289   DOI   ScienceOn
21 Gill, H. S., K. J. Rutherfurd, J. Prasad, and P. K. Gopal. 2000. Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). Br. J. Nutr. 83: 167-176   DOI   ScienceOn
22 Kato, I., T. Yokokura, and M. Mutai. 1984. Augmentation of mouse natural killer cell activity by Lactobacillus casei and its surface antigens. Microbiol. Immunol. 28: 209-217   DOI
23 Kim, J. Y., S. Lee, S. Hachimura, S. Kaminogawa, and H. J. Lee. 2003. In vitro immunopotentiating activity of cellular component of Lactococcus lactis ssp. lactis. J. Microbiol. Biotechnol. 13: 202-206