Adsorption of $\textrm{Pb}_{2+}$ in the components of bacterial cell membrane

  • Kim, Mal-Nam (Department of Biology, Sangmyung Womens University)
  • Published : 1995.12.01

Abstract

S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant $\textrm{Pb}_{2+}$ per unit dry weight of the three fractions tested. Adsorption behavior of $\textrm{Pb}_{2+}$ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more $\textrm{Pb}_{2+}$ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of $\textrm{Pb}_{2+}$ than total native cell membrane or cell membrane lipid.

Keywords

References

  1. J. Gen. Microbiol. v.128 Production and tolerance of ethanol in relation to phospholipid fatty-acyl composition in Saccharomyces cerevisiae NCYC 431 Beaven,M.J.;C.Charpentier;A.H.Rose
  2. J. Bacteriol. v.141 Sites of metal deposition in the cell wall of Bacillus subtilis Beveridge,T.J.;R.G.E.Murry
  3. Enzymes. Micro. Technol. v.16 Adsorption of metalions by Rhizopus arrhizus biomass: Charaterization studies Brady,J.M.;J.M.Tobin
  4. Biotechnol. Lett. v.17 Effect of heavy metal up-take on the electrokinetic properties of Saccharomyces cerevisiae Chen,P.;Y.P.Ting
  5. In proceedings of the 3rd Singapore Society for Microbiology International Congress Chen,P.;Y.P.Ting;W.S.Wu
  6. Biotechnol. Lett. v.17 The effect of electirc field stimulation on the biosortion of uranium by non-living biomass derived from Kiuyvermyces maxianus IMB 3 Donnellan,N.;A.Rollan;L.McHale;A.P.McHale
  7. J. Bacteriol. v.109 Interaction of concanavalin A with the cell wall of Bacillus subtilis Doyle,R.J.;D.C.Birdsell
  8. J. Bacteriol. v.143 Chemical basis for selectivity of metal ions by the Bacillus subtilis cell wall Doyle,R.J.;T.H.Matthews;U.N.Streips
  9. Appl. Biochem. Microbiol. v.30 Effect of mycelium pretreatment on lead ion adsorption by Aspergillus niger Feofilova,E.P.;A.P.Mar'in;V.M.Terashina
  10. Biotechnol. Bioeng. v.28 Biosorption of uranium and lead by Streplomyces longwoodensis Friis,N.;P.Myers-Keith
  11. Nature v.225 Teichoic acids and membrane function in bacteria Heptinstall,S.;A.R.Archbald;J.Baddiley
  12. Separation and analysis of membrane lipid components In Biological membranes a practical approach Higgins,J.A.;J.B.C.Findley(eds.);Evans,W.H.(eds.)
  13. Eur. J. Appl. Microbiol. Biotechnol. v.12 Studies on the accumulation heavy metal elements in biological systemsⅩⅨ. Accumulation of uranium by microorganisms Horikoshi,T.;A.Nakajima;T.Sakaguchi
  14. Diversity of biopolymer structure and its potential for ion binding application In Immobilisation of ions by bio-sorption Hunt,S.;H.Eccles(eds.);S.Hunt(eds.)
  15. Kor. J. Microbiol. v.30 The uptake of lead ion with Staphylococcus epidermidis Kim,J.H.;M.N.Kim
  16. Jour. Microbiol. v.33 Binding site for lead ion in Staphylococcus epidermidis Kim,M.N.;H.Y.Sung
  17. Principles of biochemistry Lehninger,A.L.
  18. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;N.J.Rosebrough;A.L.Farr;R.S.Rondall
  19. J. Biochem. v.84 Separation and characterization of the outer membrane of Pseudomonas aeruginosa Mizuno,T.;M.Kageyama
  20. Appl. Microbiol. Biotechnol. v.12 Studies on the accumulation of heavy metal element in biological systemsⅩⅦ. Selective accumulation of heavy metal ions by Chlorella regularis Nakajima,A.;T.Horikoshi;T.Sakaguchi
  21. Elements of microbiology Pelczar,M.J.;E.C.S.Chan
  22. J. Bacteriol. v.104 Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli Schnaitman,C.A.
  23. Biotechnol. Lett. v.8 Cadmium adsorption by bacterial capsular polysaccharide coatings Scott,J.A.;G.K.Sage;S.J.Palmer;D.S.Powell
  24. Appl. Environ. Microbiol. v.47 Uptake of metal ions by Rhizopus arrhizus biomass Tobin,J.M.;D.G.Cooper;R.J.Neufeld
  25. An introduction of fungal biotechnology Wainwright
  26. Endeavour New Series v.8 Zinc: What is its role in biology? Williams,R.J.P.
  27. Kor. J. Microbiol. v.25 Effect of Triton X-100 on intracellular accumulation of cadmium in Hansenula anomala Yu,T.S.;J.M.Park;H.I.Song