• Title/Summary/Keyword: cell stability

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Plasmid Propagation and Heterologous Gene Expression in Recombinant Yeast (효모균에서의 Plasmid 번식체계와 혼성유전자 발현)

  • 홍억기
    • KSBB Journal
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    • v.8 no.2
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    • pp.133-142
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    • 1993
  • The effects of genetic and environmental factors on productivity of a cloned protein were studied in recombinant Saccharomyces cerevisiae. Plasmid stability and copy level were very high for a $REP^+$ system(at ca. 10 generations, stability: 65-90%, plasmid copy number per cell: 40-200), whereas these were very low for a yep- system(at ca. 10 generations, stability: 30%, plasmid copy number per cell 20). In plasmids containing the $2{\mu}m$ circle genome, a $[cir^o]$ strain was a preferred host cell since the plasmid stability and the copy number in a $[cir^o]$ strain were higher than in a $[cir^+]$strain. Cloned gene expression was dependent on plasmid copy number and stability. The inducer (galactose) level played a very important role in cloned lacZ gene expression, showing that a galactose concentration of 0.8% was sufficient for induction of gene expression. Induction rate was very fast in the case of plasmids exhibiting high stability and copy number by a factor of 4 to 25. The time to reach the peak value of gene expression was longer when galactose was added at the start of fermentation (ca. 26 hours) than at the mid-exponential phase (ca. 6 hours). Glucose repression was reduced by a factor of 2 to 5 as the relative inducer level increased.

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Improving Power Conversion Efficiency and Long-term Stability Using a Multifunctional Network Polymer Membrane Electrolyte; A Novel Quasi-solid State Dye-sensitized Solar Cell

  • Gang, Gyeong-Ho;Gwon, Yeong-Su;Song, In-Yeong;Park, Seong-Hae;Park, Tae-Ho
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.484.2-484.2
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    • 2014
  • There are many efforts to improving the power conversion efficiencies (PCEs) of dye-sensitized solar cells (DSCs). Although DSCs have a low production cost, their low PCE and low thermal stability have limited commercial applications. This study describes the preparation of a novel multifunctional polymer gel electrolyte in which a cross-linking polymerization reaction is used to encapsulate $TiO_2$ nanoparticles toward improving the power conversion efficiency and long-term stability of a quasi-solid state DSC. A series of liquid junction dye-sensitized solar cells (DSCs) was fabricated based on polymer membrane encapsulated dye-sensitized $TiO_2$ nanoparticles, prepared using a surface-induced cross-linking polymerization reaction, to investigate the dependence of the solar cell performance on the encapsulating membrane layer thickness. The ion conductivity decreased as the membrane thickness increased; however, the long term-stability of the devices improved with increasing membrane thickness. Nanoparticles encapsulated in a thick membrane (ca. 37 nm), obtained using a 90 min polymerization time, exhibited excellent pore filling among $TiO_2$ particles. This nanoparticle layer was used to fabricate a thin-layered, quasi-solid state DSC. The thick membrane prevented short-circuit paths from forming between the counter and the $TiO_2$ electrode, thereby reducing the minimum necessary electrode separation distance. The quasi-solid state DSC yielded a high power conversion efficiency (7.6/8.1%) and excellent stability during heating at $65^{\circ}C$ over 30 days. These performance characteristics were superior to those obtained from a conventional DSC (7.5/3.5%) prepared using a $TiO_2$ active layer with the same thickness. The reduced electrode separation distance shortened the charge transport pathways, which compensated for the reduced ion conductivity in the polymer gel electrolyte. Excellent pore filling on the $TiO_2$ particles minimized the exposure of the dye to the liquid and reduced dye detachment.

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Tight adhesion of plastic substrates for cell gap stability in flexible LCDs

  • Jin, Min-Young;Kim, Sang-Il;Souk, Jun-Hyung;Kim, Jae-Hoon
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.78-81
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    • 2008
  • We developed tight adhesion techniques of two plastic substrates to maintain cell gap stability for rugged flexible LCDs. By combining rigid spacers and several adhesion materials, we demonstrated mechanically very stable flexible LCDs against pressure and bending.

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A Numerical Study of the Flame Cell Dynamics in Opposed Nonpremixed Tubular Configuration (비예혼합 튜브형상내 화염셀의 거동에 대한 수치 해석적 연구)

  • Park, Hyunsu;Yoo, Chun Sang
    • 한국연소학회:학술대회논문집
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    • 2014.11a
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    • pp.175-178
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    • 2014
  • The flame cell dynamics in 2-D opposed nonpremixed tubular configuration was investigated using high-fidelity numerical simulations. The diffusive-thermal instability occurs as the $Damk{\ddot{o}}hler$ number, Da, approaches the 1-D extinction limit of the tubular flames and several flame cells are generated depending on Da, and flame radius. In general, the number of flame cells are found close to the largest wave number from the linear stability analysis. It was also found from the displacement speed analysis that during the local flame extinction and cell formation, negative edge flame speed is observed due to small gain from reaction compared to large loss from diffusion.

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Interfacial Engineering Strategies for Third-Generation Photovoltaics (차세대 태양전지의 계면 개질 전략)

  • Lim, Hunhee;Choi, Min-Jae;Jung, Yeon Sik
    • Current Photovoltaic Research
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    • v.4 no.3
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    • pp.98-107
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    • 2016
  • Third-generation photovoltaics are of low cost based on solution processes and are targeting a high efficiency. To meet the commercial demand, however, significant improvements of both efficiency and stability are required. In this sense, interfacial engineering can be useful key to solve these issues because trap sites and interfacial energy barrier and/or chemical instability at organic/organic and organic/inorganic interfaces are critical factors of efficiency and stability degradation. Here, we thoroughly review the interfacial engineering strategies applicable to three representative third-generation photovoltaics - organic, perovskite, colloidal quantum dot solar cell devices.

Study for Thermal Stability of Liquid Crystal Device (액정 소자의 열적 안전성에 관한 연구)

  • 이상극;황정연;서대식;이준웅
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.4
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    • pp.439-442
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    • 2004
  • In this study, we investigated about electrooptics characteristic of three kinds of TN cell on the polyimide surface. Monodomain alignments of thermal stressed TN cell over temperature of liquid crystal isotropic phase were almost the same as that of no thermal stressed TN cells. However, the thermal stressed TN cells have many defects. Also, threshold voltage and response time of thermal stressed TN cells show the same performances as no thermal stressed TN cells. There were little changes of value in these TN cells. However, transmittances of TN cells on the polyimide surface decrease with increasing thermal stress time. Finally, the residual DC voltage of the thermal stressed TN cell on the polyimide surface shows decrease of characteristics as increasing thermal stress time. Therefore, the thermal stability of TN cell was decreased by high thermal stress for the long times.

Improving the Stability of Series-Connected Solid Oxide Fuel Cells by Modifying the Electrolyte Composition

  • Kim, Young Je;Lim, Hyung-Tae
    • Journal of Electrochemical Science and Technology
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    • v.12 no.1
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    • pp.159-165
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    • 2021
  • YSZ based anode supported solid oxide fuel cells (SOFCs) were prepared, and two cells with different electrolyte thicknesses were connected in series for the simulation of a cell-imbalanced fuel cell stack. Pure YSZ cells in a series connection exhibited a rapid degradation when a thick electrolyte cell was operated under a negative voltage. On the other hand, ceria added-YSZ cells in a series connection were stable under similar operating conditions, and the power density and impedance were about the same as those before tests. The improved stability was due to the reduction of internal partial pressure in the electrolyte by locally increasing the electronic conduction. Thus, we propose a new protection method, i.e., the local addition of ceria in the YSZ electrolyte, to extend the lifetime of a cell-imbalanced SOFC stack.

The Effect of the Anode Thickness on Electrolyte Supported SOFCs

  • So Yeon Shin;Dae-Kwang Lim;Taehee Lee;Sang-Yun Jeon
    • Journal of Electrochemical Science and Technology
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    • v.14 no.2
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    • pp.145-151
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    • 2023
  • Planer-type electrolyte substrates are often utilized for stack manufacturing of electrolyte-supported solid oxide fuel cells (ES-SOFCs) to fulfill necessary requirements such as a high mechanical strength and redox stability. This work did an electrochemical analysis of ES-SOFC with different NiO-YSZ anode thicknesses to find the optimal value for the high performance of the fuel cell. The cell resistivities were constant at anode thickness between 25-58 ㎛, but a thick anode (74 ㎛) caused a high electrode resistivity leading to a dramatic reduction in cell performance. A stability test was performed for 50 hours at 700℃, and the results showed a degradation rate of 0.3% per 1000 h by extrapolated fitting.

Bi-layer Electrolyte for Preventing Solid Oxide Fuel Cell Stack Degradation (고체산화물 연료전지 스택 열화 방지를 위한 전해질 기술)

  • Park, Mi Young;Bae, Hongyeul;Lim, Hyung-Tae
    • Journal of the Korean Ceramic Society
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    • v.51 no.4
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    • pp.289-294
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    • 2014
  • The stability of a solid oxide fuel cell (SOFC) stack is strongly dependent on the magnitude and profile of the internal chemical potential of the solid electrolyte. If the internal partial pressure is too high, the electrolyte can be delaminated from the electrodes. The formation of high internal pressure is attributed to a negative cell voltage, and this phenomenon can occur in a bad cell (with higher resistance) in a stack. This fact implies that the internal chemical potential plays an important role in determining the lifetime of a stack. In the present work, we fabricate planar type anode-supported cells ($25cm^2$) with a bi-layer electrolyte (with locally increased electronic conduction at the anode side) to prevent high internal pressure, and we test the fabricated cells under a negative voltage condition. The results indicate that the addition of electronic conduction in the electrolyte can effectively depress internal pressure and improve the cell stability.