• Title/Summary/Keyword: cell protection

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Protection Effects of Summer Fabrics from Cell Toxicity of UVB (직물의 자외선차단과 세포에 미치는 방호효과)

  • An, Ryeong-Mi;Lee, Su-Jin;Song, Myeong-Gyeon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.21 no.4
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    • pp.750-756
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    • 1997
  • The purpose of the study was to investigate a transmittance rate of UVB (Ultraviolet B) through summer fabrics and a protection rate of summer fabric from UVB. The subjects were randomly selected 159 fabrics from Korean common summer fabrics. The protection rates of 159 fabrics from UVB were measured by UVB lamp and UVB sensor, and 14 fabrics among these fabrics were selected for an assay of MTT(3-(4, 5-dimethylthiazol-2-yl) -2, 5 -diphenyltetrazolium). The protection rate of fabrics from cell toxicity of UVB was measured by investigating the difference of the amount of cell toxic substance on between fabrics covered with and without HeLa cell The average protection rate of 159 fabrics from UVB was 95.08%. As result findings, three negative correlations were found between: 1) the transmittance rate of UVB and the amount of MTT on fabrics (y=0.0373+0.O0518 x, r=-0.9323, p<0.001); 2) the air permeability of fabrics and the amount of MTT (r: -0.79, p< 0.01); 3) the air permeability of fabrics and the protection rate of fabrics from UVB (r=0.89, p<0.01). However, there was no effect of thickness of fabrics on the protection rate from UVB and the amount of MTT.

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Antidiabetic and Beta Cell-Protection Activities of Purple Corn Anthocyanins

  • Hong, Su Hee;Heo, Jee-In;Kim, Jeong-Hyeon;Kwon, Sang-Oh;Yeo, Kyung-Mok;Bakowska-Barczak, Anna M.;Kolodziejczyk, Paul;Ryu, Ok-Hyun;Choi, Moon-Ki;Kang, Young-Hee;Lim, Soon Sung;Suh, Hong-Won;Huh, Sung-Oh;Lee, Jae-Yong
    • Biomolecules & Therapeutics
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    • v.21 no.4
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    • pp.284-289
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    • 2013
  • Antidiabetic and beta cell-protection activities of purple corn anthocyanins (PCA) were examined in pancreatic beta cell culture and db/db mice. Only PCA among several plant anthocyanins and polyphenols showed insulin secretion activity in culture of HIT-T15 cells. PCA had excellent antihyperglycemic activity (in terms of blood glucose level and OGTT) and HbA1c-decreasing activity when compared with glimepiride, a sulfonylurea in db/db mice. In addition, PCA showed efficient protection activity of pancreatic beta cell from cell death in HIT-T15 cell culture and db/db mice. The result showed that PCA had antidiabetic and beta cell-protection activities in pancreatic beta cell culture and db/db mice.

The Sex Determination Mechanisms in Maize: Cell Death, Cell Protection and Cell Cycle Arrest (옥수수 성 결정 메커니즘: 세포 사멸, 세포 방어, 세포주기 멈춤)

  • Kim, Jong-Cheol;Lee, Kyun-Oh
    • Journal of Life Science
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    • v.16 no.4
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    • pp.699-703
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    • 2006
  • Maize (Zea mays L.) is a monoecious plant, which separates male (tassel) and female (ear) floret that evolved into increasing heterogeneity. In each floret, male or female, bears both one pistil and three stamens primodia before diverged to unisexual state. When diverged to tassel, pistil cell death occurs in the pistil primodium, which is mediated by TASSELSEED genes. In contrast, cell protection occurs in the ear pistil from TASSELSEED-mediated cell death, which is mediated by SILKLESS1 gene. On the other hand, cell cycle arrest occurred for a long time in the ear stamens and then the stamens eventually dye. The cell cycle regulating genes such as CYCLIN B and WEE1 are involved in this process. Furthermore, the temporal and spatial regulation of gibberellin biosynthesis may cause cell cycle block in arresting stamen cells. This review describes the cell death, cell protection, and cell cycle arrest mechanism during maize sex determination process at the molecular, cellular and developmental biology, and genetic levels.

The degradation of EVA for the protection of solar cell by UV-rays irradiation (자외선 조사에 따른 태양전지 보호용 EVA의 열화)

  • 김규조;연복희;김승환;김완태;허창수
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.07a
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    • pp.177-180
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    • 2000
  • We studied the degradation of EVA for the protection of solar cell by UV-rays irradiation. We investigated the reduction of electrical efficiency, photo transmmitance and degradation of EVA by UV-rays irradiation. We utilized the UV irradiation equiped with fluorescent 313nm UV lamp and radiated for 400 hours. For the chemial analysis, we used the UV-vis spectrometer, XPS and examined the degradation mechanism by UV irradiation. It is found that the discolored phenomena, the decrease of photo transmmitance and oxidation reaction is occured by UV irradiation on the EVA sample for the protection of solar cell.

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A Power MOSFET with Self Current Limiting Capability (전류 제한 능력을 갖는 전력 MOSFET)

  • 윤종만;최연익;한민구
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.32A no.10
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    • pp.25-34
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    • 1995
  • A new vertical power MOSFET with over-current protection capability is proposed. The MOSFET consists of main power MOSFET cell, sensing MOSFET cell and lateral npn bipolar transistor. The proposed MOSFET may be fabricated by a conventional DMOS process without any additional fabrication step. Overcurrent state is sensed by the newly designed lateral bipolar transistor. Mixed-mode simulations proved that the overcurrent protection is achieved by the proposed MOSFET successfully with a small protection area less than 0.2 % of the total die area.

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N-Acetylphytosphingosine Enhances the Radiosensitivity of Lung Cancer Cell Line NCI-H460

  • Han, Youngsoo;Kim, Kisung;Shim, Ji-Young;Park, Changsoe;Song, Jie-Young;Yun, Yeon-Sook
    • Molecules and Cells
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    • v.25 no.2
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    • pp.224-230
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    • 2008
  • Ceramides are well-known second messengers that induce apoptosis in various kinds of cancer cells, and their effects are closely related to radiation sensitivity. Phytoceramides, the yeast counterparts of the mammalian ceramides, are also reported to induce apoptosis. We investigated the effect of a novel ceramide derivative, N-acetylphytosphingosine (NAPS), on the radiosensitivity of NCI-H460 human lung carcinoma cells and its differential cytotoxicity in tumor and normal cells. The combination of NAPS with radiation significantly increased clonogenic cell death and caspase-dependent apoptosis. The combined treatment greatly increased Bax expression and Bid cleavage, but not Bcl-2 expression. However, there was no effect on radiosensitivity and apoptosis in BEAS2B cells, which derive from normal human bronchial epithelium. Cell proliferation and DNA synthesis were significantly inhibited by NAPS in both NCI-H460 and BEAS2B cells, but only the BEAS2B cells recovered by 48h after removal of the NAPS. Furthermore, the NCI-H460 cells underwent more DNA fragmentation than the BEAS2B cells in response to NAPS. Our results indicate that NAPS may be a potential radiosensitizing agent with differential effects on tumor vs. normal cells.

Effects of fermented Taraxacum mongolicum by Dongchimi on protection of liver cell and immune activity (포공영(민들레)의 동치미 발효에 의한 간세포 보호 및 면역증진 효과)

  • Choi, Bog-Soo;Kim, Hye-Ja
    • Journal of Society of Preventive Korean Medicine
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    • v.18 no.1
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    • pp.11-21
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    • 2014
  • Objective : This study set out to combine the treatment efficacy of Taraxacum with Dongchimi fermentation and investigate Taraxacum's effects on protection of liver cell and controlling nitric oxide(NO) through experiments, thus checking whether it had values as a physiological active matter. The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anticancer effects on liver cells, and protection effects for brain cells in the aspects of liver function and immunity enhancement. Method : The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anti-cancer effects on liver cells, and protection effects for brain cells in the aspects of liver function and immunity enhancement. Results : As shown in the chromatogram results, each valid component content increased in Taraxacum fermented by Dongchimi (TDF) for each time section. Of them, the valid component content at 36.80 minutes was approximately 2.7 times higher in TDF at 21.8% than in Taraxacum Dongchimi (TD) at 8.28%. TDF generated more excellent protection effects against the toxicity that caused oxidative damage to the liver cell(HepG2) with t-BHP than TD. The survival rate was low in TD of $100{\mu}g/m{\ell}$ and $300{\mu}g/m{\ell}$ and increased to 23.3% in TDF of $100{\mu}g/m{\ell}$. The survival rate was the highest at $300{\mu}M$ with a significant difference of 68.1%(P<0.05). Both TD and TDF showed effects of controlling nitric oxide production according to concentration with TDF recording a higher rate of controlling nitric oxide production than TD. There were significant differences(P<0.05) in the effects of controlling nitric oxide production at 200 ug/ml, 400 ug/ml in both groups. Especially the result TDF of $400{\mu}g/m{\ell}$ was thus similar to those of butein, the positive control group. Conclusion : The result of this studies is that Taraxacum fermented by Dongchimi (TDF) increased the valid component content compared with the simple mixture(TD). The findings clearly show that it is a material with the effects of improving immunity and liver cell protection. If fermentation methods are further developed to use it as a functional material, it will be subject to more opportunities of being used in other functional foods and make a contribution to integrated medicinal food development.

Design and Implementation of a Protection System for the Mentally Handicapped Using a GPS Receiver and Mobile Communications (이동통신과 GPS 수신기를 이용한 지적장애인 보호 시스템의 설계 및 구현)

  • Seol, Tae-Min;Yoon, Sang-Ho;Kang, Chang-Soon
    • Journal of Information Technology Services
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    • v.9 no.4
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    • pp.207-217
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    • 2010
  • This paper is concerned with a protection system for the mentally handicapped, which is utilizing a GPS receiver and mobile communications. The protection system consists of a location notification equipment, a location identification and management server, and a cell phone of guardian. The location notification equipment is made up of a GPS receiver, a CDMA communication module and a microprocessor. The equipment transfers the location of the mentally impaired to the location identification and management server when the handicapped gets out of a certain bound of the designated facility. The server marks the handicapped's location on the map in the server and also notifies the guardian's cell phone of the locations. With applying the proposed protection system to social welfare organizations related to the mentally handicapped, it is expected to efficiently contribute to the promotion of the handicapped's welfare.

Study on Optimization of Cytoplasm Conditions for In Vitro Micronucleus Test Using Chinese Hamster Lung Cells (Chinese Hamster Lung Cell을 이용한 in vitro 소핵시험의 세포질 최적화 연구)

  • Paik, Min Kyoung;Kim, Areumnuri;Shin, Hye Rim;Chon, Kyongmi;Park, Kyung-Hun;Ryu, Ji-Hyeok;Moon, Byeong Chul
    • Korean Journal of Environmental Agriculture
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    • v.37 no.3
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    • pp.229-234
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    • 2018
  • BACKGROUND: in vitro micronucleus test (vitMNT) is one of the promising alternative testing methods in genotoxicity test and was adopted as OECD test guideline for chemical registration. This study was conducted to optimize the cytoplasm conditions in vitMNT using Chinese hamster lung (CHL) cell. METHODS AND RESULTS: In this study cytokinesis-block micronucleus test was conducted. Mitomycin C and colchicine were used as positive control chemicals and were treated for three hours (short time) or twenty-four hours (long time). Giemsa solution was used for cell staining. For optimization of vitMNT, the final fixative was prepared as five concentrations (0%, 1%, 3%, 5%, and 25%) of acetic acid in methanol, and treatment times of the final fixative were varied under four conditions (immediately, one hour, four hours, and one day). CONCLUSION: Acetic acid at 1% in methanol as the final fixative was most adequate to preserve the cytoplasm around the nucleus in the interphase cells. Also, fixative treatment time of cell suspension for one to four hours may minimize the cell rupture. These results can be helpful for getting an accurate result promptly due to clear visual distinction to score micronucleus in vitMNT using giemsa solution.

Cadaverine is Transported into Vibrio vulnificus Through its CadB in Alkaline Environment

  • Kang, In-Hye;Kim, Eui-Jin;Lee, Jeong-K.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.10
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    • pp.1122-1126
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    • 2009
  • The exogenously added cadaverine is effective in protecting Vibrio vulnificus from methyl viologen (MV)-induced superoxide stress at pH 8.5. Such a protective effect by cadaverine was not observed at pH 7.5. Consistently, the accumulated level of intracellular cadaverine at pH 8.5 is approximately four times as much as that of the control cell at pH 7.5. Cadaverine accumulation is not affected by MV. The protection of V. vulnificus by cadaverine from superoxide stress was abolished when cadB coding for the lysine-cadaverine antiporter was interrupted. However, the cadaverine-mediated protection was complemented with cadB DNA. Therefore, CadB of V. vulnificus not only acts as a lysine-cadaverine antiporter at acid pH to neutralize the external medium, but also mediates cadaverine uptake at alkaline pH to result in cell protection from superoxide stress.