• 제목/요약/키워드: cell monitoring

검색결과 720건 처리시간 0.031초

통합 연료전지모니터링 시스템 개발 (Development of the Integrated Fuel Cell Monitoring System)

  • 김현준;염상철;안병기;김세훈;금영범
    • 한국수소및신에너지학회논문집
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    • 제26권3호
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    • pp.241-246
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    • 2015
  • The interest of New Renewable Energy is increasing globally because of the increment of the uncertainty for the energy's supply and demand, and the increment of the frequency in weather anomaly and its damages. One of the New Renewable Energies, Hydrogen receives attention as the future energy that can deal with global environment regulation. Fuel Cell Electric Vehicle (FCEV) is an environment-friendly vehicle that uses Hydrogen as fuel. The electric power for FCEV is generated by chemical reaction with Oxygen from the air and Hydrogen. Hyundai Motor Company (HMC) has developed a proprietary fuel cell system since 2005. In 2012, HMC is the first car maker that mass-produces the ix35 FCEV to the worldwide such as North America, Europe, etc. In order to develop and improve the FCEV technology, data acquisition and analysis of the driving vehicle information is essential. Therefore, the monitoring system is developed, which is consist of datalogger, Automatic Vehicle Location (AVL) server and main server. Especially, WCDMA technology is integrated into the system which enables the data analysis without any restriction of time and region. The main function of the system is the analysis of the driving pattern and the component durability, and the safety monitoring. As a result, ix35 FCEV has successfully developed by using the developed monitoring system. The system is going to take an advantage of development in the future FCEV technology.

마이크로플레이트 기반 생물반응기 시스템(MABOOMSTM)을 이용한 발효배지 성분의 미생물 성장 특성 연구 (Study on Cell Growth Characteristics with Culture Medium Components by Using MABOOMSTM)

  • 손옥재;이종일
    • KSBB Journal
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    • 제28권1호
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    • pp.31-35
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    • 2013
  • In this work a $MABOOMS^{TM}$ has been employed to cultivate microorganisms and investigated the effects of culture medium components on cell growth. A 24-well microplate coated with 4-divided fluorescent sensing membranes was used to monitor the dissolved oxygen, pH and cell concentration during cultivations. Fluorescence intensity for dissolved oxygen or solution pH and reflectance for cell concentration was online monitored by using the $MABOOMS^{TM}$. The online monitoring results showed the effects of culture medium components on cell growth in cultivation processes very well.

장애인 및 노약자를 위한 WAP 기반 행동 모니터링 시스템 (Behavior Monitoring System based on WAP for the Elderly and the Disabled)

  • 김택현;이희영
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2003년도 하계종합학술대회 논문집 V
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    • pp.2565-2568
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    • 2003
  • This paper presents a behavioral data monitoring system based on WAP(wireless application protocol) service for the 24-hour continuous health state monitoring of the elderly and the disabled. The developed system transmits a character message to the predefined mobile cell phone through SMS service when an emergency state takes place. Simultaneously, the image captured by a CCD camera is transmitted to the server computer installed WAP service program. Then, the user of the cell phone who received he message can access the server and open the transmitted image. This system can be used for the effective health monitoring of the elderly and disabled.

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부식촉진시험에 의한 콘크리트 내의 철근의 부식 모니터링에 관한 실험적 연구 (An Experimental Study on the Corrosion Monitoring of Reinforcing Steel in Concrete by the Accelerated Corrosion Test)

  • 배수호;정영수;김진영
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2001년도 가을 학술발표회 논문집
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    • pp.543-548
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    • 2001
  • The corrosion monitoring methods of reinforcing steel in concrete are the various methods such as half cell potential method, galvanic current method, resistivity method, polarization resistance method, AC impedance method and etc. In this study, the corrosion monitoring methods of reinforcing steel in concrete were investigated for the test specimens using corrosion inhibitors, zinc-mortar, zinc-plate, respectively. For this purpose, the accelerated corrosion tests for reinforcing steel were conducted according to the periodic cycles(140 days) of wetting($65^{\circ}C$, 90% R.H.) and drying period(15$^{\circ}C$ , 65% R.H.) for the test specimens. As a result, it can be concluded from the test that half cell potential and galvanic current method as monitoring techniques for corrosion were found to be relatively reliable and easily usable method in the field.

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식품공정의 에너지 절감과 품질향상에 관한 연구(1). 세선 가열법의 식품 생물공학에의 응용 (On Energy Saving and Quality Improvement of Food Process (1). Applications of Hotwire Monitoring System for Food Biotechnology)

  • 허종화;크라우
    • KSBB Journal
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    • 제5권4호
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    • pp.403-410
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    • 1990
  • Application of the Rheocatch Hotwire Monitoring System for food biotechnology process was evaluated. The growth of microogranism, E coli (JM 83 and Sigma) and Corynesccfertun glutamicum, were monitored. in the fermentor. The cell growth could not be detected the temperature differences between the hotwire and samples($\Delta$T) as indicated by the monitoring system during the fermentation processes. The cell concentration of less than 2g/dl was not sufficient to generate the measurable temperature difference in the fermentor. In order to calibrate the Rheocatch Monitoring System, the temperature difference as a function of solute concentration (microbial cells, sodium cholide, sucrose and dextran) was studied. The relationship between $\Delta$T and the concentration of microbial cells, sucrose and dextran can be expressed in a power series. Further studied with dextran indicated that viscosity and/or kinematic viscosity increase exponentially with an increase in $\Delta$T This is regardless of the concentration and molecular weight of dextran. $\Delta$T linearly increases with the logarithm of molecular weight, while the logarithm of viscosity and the logarithm of kinematic viscosity increase with the logarithm of molecular weight.

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특이 환경오염물질 검출을 위한 미생물 세포 바이오센서의 활용 (Applications of Microbial Whole-Cell Biosensors in Detection of Specific Environmental Pollutants)

  • 신혜자
    • 생명과학회지
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    • 제21권1호
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    • pp.159-164
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    • 2011
  • 미생물 세포 바이오센서는 환경오염물질의 모니터링을 위한 좋은 분석도구가 될 수 있다. 이는 리포터유전자들(예로, lux, gfp or lacZ)을 방향족 화합물이나 중금속과 같은 오염물질에 반응하는 유도 조절유전자와 결합하여 만든다. 이러한 유전자 재조합기술을 이용하여 많은 종류의 미생물 바이오센서가 개발되었으며 환경, 의학, 식품, 농업, 및 방위등 다양한 분야에서 활용되고 있다. 또한 바이오센서의 민감도와 검출범위는 조절유전자의 변형을 통해 증가시킬 수있다. 최근에는 미생물 바이오센서 세포를 고효율 검색용 세포 에레이의 칩, 광섬유 등에 고착하여 활용하고 있다. 본 논문은 특이 오염물질의 검출을 위한 유전자 재조합으로 만든 미생물 세포 바이오센서의 현황과 미래에 대해 고찰한다.

Electrochemical Monitoring of NADH Redox with NPQD-modified Electrodes for Cell Viability Assessment

  • JuKyung Lee;Hye Bin Park;Chae Won Seo;Chae Won Seo;SangHee Kim
    • 센서학회지
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    • 제32권6호
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    • pp.412-417
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    • 2023
  • There is increasing interest in the rapid and highly sensitive monitoring of cell viability in biological and toxicological research. Conventional methods depend on optical assays using Water Soluble Tetrazolium-8 (WST-8) or 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, which requires a large volume of samples and special instruments, necessitating shipment of clinical samples to laboratories. This paper reports on the development of a rapid and sensitive electrochemical (EC) sensor using screen printed electrode (SPE) and surface modification using 4'-mercapto-N-phenylquinone diamine (4'-NPQD), as double electron mediators, for monitoring cell viability via the measurement of nicotinamide adenine dinucleotide (NADH). We used the sensor to observe the viability of MCF-7 and doxorubicin (Dox)-treated cells. The oxidation current of NADH was measured via chronoamperometry (CA), and the EC results showed a good linear relationship when compared with NADH quantification using WST-8 assay. The analysis time was only 10 s and limit of detection (LOD) of NADH was 1.78 µM. Our EC method has the potential to replace conventional WST assays for cell viability and cytotoxicity experiments.

유해대기오염물질 모니터링을 위한 대도시 우선순위 측정지점 선정기법 제안 (Suggestions on the Selection Method of Priority Monitoring Sites for Hazardous Air Pollutants in Megacities)

  • 권혜옥;김성준;김용표;김상균;홍지형;최성득
    • 한국대기환경학회지
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    • 제33권6호
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    • pp.544-553
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    • 2017
  • There is an overall guideline of the installation of air quality monitoring stations in Korea, but specified steps for the selection of monitoring sites for hazardous air pollutants(HAPs) are not provided. In this study, we proposed a systematic method for the selection of monitoring sites for HAPs using geographic information system (GIS). As a case study, the Seoul metropolitan area (Seoul, Incheon, and Gyeonggi Province) was chosen, and 15 factors including population, vehicle registration, and emission data were compiled for each grid cell ($7km{\times}7km$). The number of factors above the top 30% of individual data for each grid cell was used to select priority monitoring sites for HAPs. In addition, several background sites were added for data comparison and source identification. Three scenarios were suggested: Scenario 1 with 7 sites, Scenario 2 with 17 sites, and Scenario 3 with 30 sites. This proposal is not the final result for an intensive monitoring program, but it is an example of method development for selecting appropriate sampling sites. These results can be applied not only to HAPs monitoring in megacities but also to the national HAPs monitoring network.

연료전지 셀 전압 측정 장치 개발에 관한 연구 (Study about the development of voltage measurement device for fuel cell stack)

  • 김태후;정재욱;문희춘;전윤석
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2007년도 춘계학술대회
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    • pp.569-572
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    • 2007
  • In this paper, research about SVM(stack voltage monitoring) module is written, which studied to detect the failure mode of stack and stop stack driving. It is important role for SVM module to monitor the cell voltage and also, transfer those data to Supervisor controller. SVM module needs accurate measurement to detect failure mode, because the cell voltage is very small value under a few [V]. For improving these cost and technical efficiency, the electric characteristic experiment is made with the measurement circuit designed by using precision resistor.

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A comparative study of three different viability tests for chemically or thermally inactivated Escherichia coli

  • Park, Seon Yeong;Kim, Chang Gyun
    • Environmental Engineering Research
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    • 제23권3호
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    • pp.282-287
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    • 2018
  • Three different methods of bacterial viability monitoring were compared to detect chemically or thermally inactivated Escherichia coli. Direct colony enumeration, live/dead bacterial cell staining with a fluorescent dye, and the dehydrogenase activity assay were compared with respect to their ease of use and time required to perform the three different tests. The green (live cell)/red (dead cell) ratio obtained from the fluorescent bacterial cell staining approach showed a linear relationship with the colony forming units; the result obtained with dehydrogenase was similar to those. The sensitivity of the monitoring methods to detect bacterial deactivation varied with different disinfection conditions. After thermal treatment, the sensitivity of the staining approach was lower, while that of the dehydrogenase activity assay was the highest. After chemical treatment, the sensitivity of detection for both methods was similar.