KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Study on Cell Growth Characteristics with Culture Medium Components by Using MABOOMSTM

마이크로플레이트 기반 생물반응기 시스템(MABOOMSTM)을 이용한 발효배지 성분의 미생물 성장 특성 연구

  • Sohn, Ok-Jae (School of Applied Chemical Engineering, Research Institute for Catalysis, Chonnam National University) ;
  • Rhee, Jong Il (School of Applied Chemical Engineering, Research Institute for Catalysis, Chonnam National University)
  • 손옥재 (전남대학교 공과대학 응용화학공학부, 촉매연구소) ;
  • 이종일 (전남대학교 공과대학 응용화학공학부, 촉매연구소)
  • Received : 2012.12.10
  • Accepted : 2013.02.15
  • Published : 2013.02.27
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Abstract

In this work a $MABOOMS^{TM}$ has been employed to cultivate microorganisms and investigated the effects of culture medium components on cell growth. A 24-well microplate coated with 4-divided fluorescent sensing membranes was used to monitor the dissolved oxygen, pH and cell concentration during cultivations. Fluorescence intensity for dissolved oxygen or solution pH and reflectance for cell concentration was online monitored by using the $MABOOMS^{TM}$. The online monitoring results showed the effects of culture medium components on cell growth in cultivation processes very well.

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References

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