• KSBB Journal
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• 제28권1호
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• pp.24-30
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• 2013

Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a $MABOOMS^{TM}$ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru ${(dpp)_3}^{2+}$ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.904-910
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• 2009

The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide-functionalized silver and gold nanoparticles by surface-enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at ${\sim}2,100cm^{-1}$ in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and $3,600cm^{-1}$. The SERS marker band at ${\sim}2,100cm^{-1}$ could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권4호
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• pp.304-308
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• 2005

The response of IPTG induction was investigated through the monitoring of the alkali consumption rate and buffer capacity during the cultivation of recombinant E. coli BL21 (DE3) harboring the plasmid pRSET-LacZ under the control of lac promoter. The rate of alkali consumption increased along with cell growth, but declined suddenly after approximately 0.2 h of IPTG induction. The buffer capacity also declined after 0.9 h of IPTG induction. The profile of buffer capacity seems to correlate with the level of acetate production. The IPTG response was monitored only when introduced into the mid-exponential phase of bacterial cell growth. The minimum concentration of IPTG for induction, which was found out to be 0.1 mM, can also be monitored on-line and in-situ. Therefore, the on-line monitoring of alkali consumption rate and buffer capacity can be an indicator of the metabolic shift initiated by IPTG supplement, as well as for the physiological state of cell growth.

• 한국정보통신학회논문지
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• 제20권12호
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• pp.2235-2242
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• 2016

연료전지 기반의 전력시스템이 선박 및 해양플랜트에 다양한 형태로 적용되고 있다. 특히, 연료전지와 배터리를 연계한 토폴로지에 관한 하이브리드 전력시스템에 관한 연구가 활발히 이루어지고 있다. 이러한 연료전지 기반의 하이브리드 선박은 개발 초기 단계로 통합 모니터링 시스템에 연구가 이루어지고 있지 않다. 본 논문에서는 통합 모니터링 시스템을 연구하여 하이브리드 연료전지 선박의 안정적인 운항 및 운항자 편리성을 높이고자 한다. 연료전지 선박의 통합 모니터링 시스템은 사용자 편의성 및 선박 운항 안정성을 고려하여 GUI(Graphic User Interface)기반으로 구성하였으며, 선박 주요장비와 통신 및 하드와이어 신호로 연동되어 선박의 상태를 실시간으로 확인할 수 있도록 한다. 수집된 데이터는 저장하여 추후 운항자가 선박 운항 상태를 확인할 수 있도록 구현하였다.

• 한국자동차공학회논문집
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• 제22권7호
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• pp.76-83
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• 2014

During PEMFC(Proton Exchange Membrane Fuel Cell) operation monitoring and diagnosis are important issues for reliability and durability. Stack defect can be followed by a critical cell voltage drop in the stack. One method for monitoring the cell voltage is CVM(Cell Voltage Monitoring), where all cells in the stack are electrically connected to a voltage measuring system and monitored these voltages. The other methods are based on the EIS(Electrochemical Impedance Spectroscopy) and on nonlinear frequency response. In this paper, intermodulation(IM) method for diagnosis PEMFC stack is introduced. To detect one or more critical PEMFC cell voltage PEMFC stack is excited by two or more test sinusoid current, and the frequency response of the stack voltage is analyzed. If one or more critical cell voltage exists, higher harmonics on the voltage frequency spectrum will appear. For the proposed IM method, stack simulation and experiments are conducted.

• 한국위성정보통신학회논문지
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• 제8권3호
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• pp.15-19
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• 2013

리튬이온 배터리는 낮은 자체 방전율 특성과 고밀도 에너지 저장장치로 다양하게 응용되고 있으며, 단위 셀 배터리의 전압은 4V 보다 낮아, 직렬로 연결하여 사용해야 하는 것이 일반적이다. 배터리 셀전압의 직렬 연결 동작시, 각각의 단위 셀 배터리는 내부저항이 균일하지 않아, 충전 시, 특정 단위 셀 배터리는 4V 이상이 걸려, 폭발이 발생할 수도 있으며, 또한, 방전 시 배터리의 특성을 떨어뜨릴 수 있는 한계 전압 이하가 되는 심각한 문제가 존재한다. 따라서, 단위 셀 배터리의 충전 및 방전 동작에서는 과충전과 과방전을 사전에 감지하기 위한 전압 센싱 동작이 필요하며, 이에, 본 논문에서는 고속 배터리 전압 센싱 모듈 개발을 소개한다. 제작된 CVM(Cell Voltage Monitoring)은 단위 배터리 셀을 통하여, 전압 및 온도 채널 포함하여, 12채널을 모니터링할 수 있으며, 채널당 12-bits 분해능과 192 kbps 전송 속도를 가진다.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.268-275
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• 2004

To investigate the potential application of the single-cell gel electrophoresis (SCGE) assay to carp as an aquatic pollution monitoring technique, gill, liver, and blood cells were isolated from carp exposed to a direct-acting mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), or indirect mutagen, $benzo[\alpha]pyrene$ $(B[\alpha]P)$, then the DNA strand breakage was analyzed using the assay. Based on testing 5 different cell isolation methods and 6 electrophoretic conditions, the optimized assay conditions were found to be cell isolation by filter pressing and electrophoresis at a lower voltage and longer running time (at 0.4 V/cm for 40 min). In preliminary experiments, gill and liver cells isolated from carp exposed to MNNG in vitro exhibited DNA damage signals even with 0.5 ppb exposure, which is a much higher dose than previously reported. In the gill cells isolated from carp exposed to 0.01-0.5 ppm MNNG in vivo, significant dose-and time-dependent increases were observed in the tail for 4 days. As such, the linear correlation between the relative damage index (RDI) values and time for each dose based on the initial 48-h exposure appeared to provide effective criteria for the genotoxicity monitoring of direct-acting mutagenic pollution. In contrast, the in vivo exposure of carp to 0.25-1.0 ppm of $B[\alpha]P$ for 7 days resulted in dose-and time-dependent responses in the liver cells, in which 24-h delayed responses for metabolizing activation and gradual repair after 48 h were also observed. Thus, the negative-sloped linear correlation between the RDI and time at each dose based on the initial 48 h appeared to provide more effective criteria for the genotoxicity monitoring of indirect mutagenic pollution.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2015년도 전력전자학술대회 논문집
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• pp.401-402
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• 2015

In this paper a direct cell-to-cell charge balancing circuit which can transfer the charge from any cell to any cell in the battery string is introduced. In the proposed topology the energy in the high voltage cell is transferred to the low voltage cell through the simple operation of a dc-dc converter to get fast equalization. Furthermore, the charge equalization can be performed regardless of the battery module operation whether it is being charged, discharged or relaxed. The monitoring circuit composed of a DSP and a battery monitoring IC is designed to monitor the cell voltage and protect the battery. In order to demonstrate the advantages of the proposed topology, a prototype circuit was designed and applied to 12 Lithium-Ion battery module. It has been verified with the experiments that the charge equalization time of the proposed method was shortest compared with those of other methods.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1995년도 추계학술대회 논문집
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• pp.1182-1185
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• 1995

This paper presents a novel technology for unattented continuous monitoring of radioactive material in hot cell environments. In this monitoring system, the surveillance camera data and NDA data are time synchronized and integrated into the same dimension through data processing. The integrated information is then fed into a neural network to generate diagnostics through data processing. the integrated information of the concept is tested for a spent nuclear fuel transprotation in an operational hot cell at KAERI. The presented integral part of the multi-sensory system and the analytical paradigm may provide an effective technologyical alternative for safeguarding new conceptual hot cell facilities, namely the Dupic facility.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2015년도 추계학술대회 논문집
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• pp.7-8
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• 2015

In this paper a cell-to-cell fast charge balancing circuit for the Lithium-Ion battery module is proposed. In the proposed topology the energy in a high voltage cell is transferred directly to a low voltage cell through the operation of the dc-dc converter. Furthermore, the charge balancing can be performed regardless of the battery operation whether it is being charged, discharged or relaxed. The monitoring circuit composed of a DSP and a battery monitoring IC is designed to monitor the cell voltage and detect the inferior cell thereby protecting the battery module from failure. In order to demonstrate the performance of the proposed topology, a prototype circuit was designed and applied to 12 Lithium-Ion battery module. It has been verified with the experiments that the charge equalization time of the proposed method was shorter compared with those of other methods.