• Title/Summary/Keyword: cell monitoring

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Implementation of a CAN Based Real-Time Simulator for FCHEV (하이브리드 연료전지 자동차의 CAN기반 실시간 시뮬레이터 구현)

  • Shim, Seong-Yong;Lee, Nam-Su;Ahn, Hyun-Sik;Kim, Do-Hyun
    • Proceedings of the KIEE Conference
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    • 2004.11c
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    • pp.410-413
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    • 2004
  • In this paper, a simulator system for Fuel Cell Hybrid Electric Vehicles(FCHEV) is implemented using DSP boards with CAN bus. The subsystems of a FCHEV i.e., the fuel cell system, the battery system, the vehicle dynamics with the transmission mechanism are coded into 3 DSP boards. The power distribution control algorithm and battery SOC control are also coded into a DSP board. The real-time monitoring program is also developed to examine the control performance of power control and SOC control algorithms.

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The Effect of Air Impurities on the PEMFC Performances (공기에 포함된 불순물에 의한 PEMFC 운전 성능 변화)

  • Jang, Jong-Hyun;Kim, Yi-Young;Han, Jong-Hee;Lee, Sang-Yeop;Cho, Eun-Ae;Kim, Hyoung-Juhn;Lim, Tae-Hoon
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.526-529
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    • 2008
  • The effect of air impurities on PEMFC performances were studied using electrochemical analysis, such as OCV monitoring, polarization, constant current operation, and electrochemical impedance spectroscopy. The nitrogen dioxide in air lowered the operation voltage at 1 A/$cm^2$ by 160 mV (10 ppm) and 227 mV (100 ppm), while the carbon monoxide effect was relatively not significant (30 mV at 100 ppm). For both nitrogen dioxide and carbon monoxide, the performances were largely recovered when pure air was provided again. Further study for additional air impurities and simulated air are under progress to provide fundamental data for the design of fuel cell vehicles.

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In Situ Fluorescence Optical Detection Using a Digital Micromirror Device (DMD) for 3D Cell-based Assays

  • Choi, Jong-Ryul;Kim, Kyujung;Kim, Donghyun
    • Journal of the Optical Society of Korea
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    • v.16 no.1
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    • pp.42-46
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    • 2012
  • We have developed a fluorescence optical detection system using a digital micromirror device (DMD) for monitoring 3D cell culture matrices in situ. Full 3D imaging with fast scanning speed was implemented by the combined action of a DMD and a motorized stage. Imaging results with fluorescent microbeads measure the minimum axial resolution of the system as $6.3{\mu}m$, while full 1-mm scanning through 3D alginate-based matrix was demonstrated. For cell imaging, improved images were obtained by removing background fluorescence although the scanning distance was reduced because of low intracellular fluorescence efficiency. The system is expected to be useful to study various dynamics and behaviors of 3-dimensionally cultured cells in microfluidic systems.

Oxygen Transfer Rate Coefficient of Membrane Aeration Bioreactor for Vero Cell Culture

  • Jeon, Ju-Mi;Jeong, Yeon-Ho;Kim, Ik-Hwan;Lee, Sang-Jong;Jang, Yong-Geun;Jeon, Gye-Taek
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.269-270
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    • 2002
  • Oxygen is a key substrate in animal cell metabolism and its consumption is thus a parameter of great interest for monitoring and control in animal cell culture bioreactor. The use of a gas-permeable membrane offered the possibility to provide the required quantity of oxygen into the culture. while avoiding problems of foaming or shear damage generally linked to sparging. For determining the optimum DO control strategy of this gas-permeable membrane aeration bioreactor, the oxygen transfer rate coefficient was measured with varying $N_2$ ratio in inlet air. The results showed that an increasing mass flow rate of nitrogen reduced the $K_La$ value. and 5% nitrogen in air did not result in any oxygen limitation.

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Analysis of chromaticity cause in Jeju Eoseungsaeng Lake (제주도 어승생 저수지 색도 원인 분석)

  • Lee, Jeonghoon;Lee, Heenam;Kim, Jinkeun
    • Journal of Korean Society of Water and Wastewater
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    • v.30 no.4
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    • pp.381-389
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    • 2016
  • Jeju Eoseungsaeng lake which is a main water resource of Eoseungsaeng water treatment plant($Q=15,000m^3/d$) experienced high chromaticity(40 CU) and pH(9.46) in 2013. This could decline customer's confidence on drinking water quality unless proper identification and removal of chromaticity were implemented. To find cause of chromaticity, water monitoring on various water parameters including TOC, algal cell count, Chl-a, turbidity, SS, conductivity, etc. were implemented. Iron and manganese were excluded from the cause of chromaticity due to its low concentration (i.e., < 0.02 mg/L). Correlation among water parameters showed that relationship between algal cell count and chromaticity was the highest(R=0.43), which suggested that presumably the main reason of chromaticity occurrence in Jeju Eoseungsaeng lake was algae.

Estimation of Cell Concentration by Light Transmitter During the Culture of Methylotrophic Yeast Pichia pastoris

  • Choi, Du-Bok;Park, Enoch Y.S.;Lee, Yong-Bo;Na, Young-Hee;Lim, Chae-Kyu
    • Mycobiology
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    • v.31 no.4
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    • pp.226-228
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    • 2003
  • The multiple correlation coefficient between the values determined by dry weight and those determined by fluorometer was observed with r=0.96 and the standard error of calibration was 0.034. Using the best calibration data, in order to reconfirm the reliability of the fluorometer results in comparison with those obtained by dry weight on the cell concentration, fedbatch cultures were carried out. The results obtained by fluorometer measurements were in good agreement with those obtained by dry weight. The on-line monitoring of cell concentration by the fermentor system linked to a computer equipped with fluorometer was successfully carried out.

Quantitative Analysis of Indomethacin by the Portable Near-Infrared (NIR) System (근적외분광분석법을 이용한 인도메타신의 정량분석)

  • 김도형;우영아;김효진
    • YAKHAK HOEJI
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    • v.47 no.5
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    • pp.261-265
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    • 2003
  • Near-infrared (NIR) system was used to determine rapidly and simply indomethacin in buffer solution for a dissolution test of tablets and capsules. Indomethacin standards were prepared ranging from 10 to 50 ppm using the mixture of phosphate buffer (pH 7.2) and water (1 : 4). The near-infrared (NIR) transmittance spectra of indomethacin standard solutions were collected by using a quartz cell in 1 mm and 2 mm pathlength. Partial least square regression (PLSR) was explored to develop calibration models over the spectral range 1100∼1700 nm. The model using 1 mm quartz cell was better than that using 2 mm quartz cell. The PLSR models developed gave standard error of prediction (SEP) of 0.858 ppm. In order to validate the developed calibration model, routine analysis was performed using another standard solutions. The NIR routine analysis showed good correlation with actual values. Standard error of prediction (SEP) is 1.414 ppm for 7 indomethacin samples in routine analysis and its error was permeable in the regulation of Korean Pharmacopoeia (VII). These results show the potential use of the real time monitoring for indomethacin during a dissolution test.

In Situ Monitoring of Biofilm Formations of Escherichia coli and Pseudomonas putida by Use of Lux and GFP Reporters

  • Khang, Youn-Ho;Rober S. Burlage
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.3 no.1
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    • pp.6-10
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    • 1998
  • A plasmid vector containing two reporter genes, mer-lux and lac-GFP, was transformed to both Escherichia coli and Pseudomonas putida. Their cellular activities and biofilm characteristics were investigated in flow-cell units by measuring bioluminescent lights and fluorescent levels of GFP. Bioluminescence was effective to monitor temporal cell activities, whereas fluorescent level of GFP was useful to indicate the overall cell activities during biofilm development. The light production rates of E. coli and P. putida cultures were dependent upon concentrations of HgCl2. Mercury molecules entrapped in P. putida biofilms were hardly washed out in comparison with those in E. coli biofilms, indicating that P. putida biofilms may have higher affinity to mercury molecules than E. coli biofilms. It was observed that P. putida expressed GFP cDNA in biofilms but not in liquid cultures. This may indicate that the genetic mechanisms of P. putida were favorably altered in biofilm conditions to make a foreign gene expression possible.

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Photovoltaic System Output Forecasting by Solar Cell Conversion Efficiency Revision Factors (태양전지 변환효율 보정계수 도입에 의한 태양발전시스템 발전량 예측)

  • Lee Il-Ryong;Bae In-Su;Shim Hun;Kim Jin-O
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.54 no.4
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    • pp.188-194
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    • 2005
  • There are many factors that affect on the system output of Photovoltaic(PV) power generation; the variation of solar radiation, temperature, energy conversion efficiency of solar cell etc. This paper suggests a methodology for calculation of PV generation output using the probability distribution function of irradiance, PV array efficiency and revision factors of solar cell conversion efficiency. Long-term irradiance data recorded every hour of the day for 11 years were used. For goodness-fit test, several distribution (unctions are tested by Kolmogorov-Smirnov(K-S) method. The calculated generation output with or without revision factors of conversion efficiency is compared with that of CMS (Centered Monitoring System), which can monitor PV generation output of each PV generation site.

A Study of Monitoring and Operation for PEM Water Electrolysis and PEM Fuel Cell Through the Convergence of IoT in Smart Energy Campus Microgrid (스마트에너지캠퍼스 마이크로그리드에서 사물인터넷 융합 PEM 전기분해와 PEM 연료전지 모니터링 및 운영 연구)

  • Chang, Hui Il;Thapa, Prakash
    • Journal of the Korea Convergence Society
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    • v.7 no.6
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    • pp.13-21
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    • 2016
  • In this paper we are trying to explain the effect of temperature on polymer membrane exchange water electrolysis (PEMWE) and polymer membrane exchange fuel cell (PEMFC) simultaneously. A comprehensive studying approach is proposed and applied to a 50Watt PEM fuel cell system in the laboratory. The monitoring process is carried out through wireless LoRa node and gateway network concept. In this experiment, temperature sensor measure the temperature level of electrolyzer, fuel cell stack and $H_2$ storage tank and transmitted the measured value of data to the management control unit (MCU) through the individual node and gateway of each PEMWE and PEMFC. In MCU we can monitor the temperature and its effect on the performance of the fuel cell system and control it to keep the lower heating value to increase the efficiency of the fuel cell system. And we also proposed a mathematical model and operation algorithm for PEMWE and PEMFC. In this model, PEMWE gives higher efficiency at lower heating level where as PEMFC gives higher efficiency at higher heating value. In order to increase the performance of the fuel cell system, we are going to monitor, communicate and control the temperature and pressure of PEMWE and PEMFC by installing these systems in a building of university which is located in the southern part of Korea.