• Title/Summary/Keyword: cell culture model

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Streamlined Shape of Endothelial Cells

  • Chung, Chan-Il;Chang, Jun-Keun;Min, Byoung-Goo;Han, Dong-Chul
    • Journal of Mechanical Science and Technology
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    • v.14 no.8
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    • pp.861-866
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    • 2000
  • Flow induced shape change is important for spatial interpretation of vascular response and for understanding of mechanotransduction in a single cell. We investigated the possible shapes of endothelial cell (EC) in a mathematical model and compared these with experimental results. The linearized analytic solution from the sinusoidal wavy wall and Stokes flow was applied with the constraint of EC volume. The three dimensional structure of the human umbilical vein endothelial cell was visualized in static culture or after various durations of shear stress (20 $dyne/cm^2$ for 5, 10, 20, 40, 60, 120min). The shape ratio (width: length: height) of model agreed with that of the experimental result, which represented the drag force minimizing shape of stream-lining. EC would be streamlined in order to accommodate to the shear flow environmented by active reconstruction of cytoskeletons and membranes through a drag force the sensing mechanism.

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Anti-inflammatory Effects of Haepyoijin-tang in Aspergillus Oryzae Protease Induced Respiratory Inflammation Model (Aspergillus oryzae protease 유도 호흡기 염증모델에서 해표이진탕(解表二陳湯)의 항염증 효과)

  • Bo-In Kwon;Joo-Hee Kim
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.38 no.1
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    • pp.16-21
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    • 2024
  • Haepyoijin-tang and its main components have been used for phlegm, cough and dyspnea. Using a respiratory inflammation model, we intend to reveal the anti-inflammatory effect and pharmacological mechanism of Haepyoijin-tang. We induced the respiratory inflammation model by Aspergillus oryzae protease and ovalbumin administration. Female Balb/c mice (8 weeks old) were classified into four groups as follows: saline control group, aspergillus oryzae protease and ovalbumin induced respiratory inflammation group (vehicle), inflammation with Haepyoijin-tang (200 mg/kg) administration group, inflammation with dexamethasone (5 mg/kg) administration group (n=7). To identify the anti-inflammatory effects of Haepyoijin-tang water extracts, we measured the inflammatory cell number in bronchoalveolar lavage fluid (BALF) and total live lung cell number. In addition, we checked eosinophil ratio and number in BALF. And Interleukin (IL)-5 level was also measured in lung cell culture supernatant. To confirm the mechanism of anti-inflammatory effects, we analyzed the activated helper T cell (CD4+CD25+ cell) and Th2 cell (CD4+GATA3+ cell) ratio and number in lung by using flow cytometry. Finally, we attempted to confirm the immune mechanism by measuring the ratio and number of regulatory T cells (CD4+Foxp3+ cell). Haepyoijin-tang extracts treatment diminished inflammatory cell, especially, eosinophil number in BALF and total live lung cell number. Moreover, IL-5 level was reduced in Haepyoijin-tang treated group. Surprisingly, Haepyoijin-tang extracts administration not only decreased the activated helper T cell but also Th2 cell population in lung. Additionally, regulatory T cell population was increased in Haepyoijin-tang administration group. Our findings proved that Haepyoijin-tang extract have anti-inflammatory efficacy by suppressing Th2 cell activation and promoting regulatory T cell population.

The Effect of Injinchunggan-tang on Hepatitis C Virus Infection (인진청간탕이 C형 간염 바이러스의 임파구계 세포감염에 미치는 영향)

  • 김선민;이장훈;우홍정
    • The Journal of Korean Medicine
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    • v.22 no.1
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    • pp.53-62
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    • 2001
  • Objective : The aim of this study was to evaluate the efficacy of Injinchunggan-tang on Hepatitis C virus infection, and to clarify the mechanism of treatment by indentifying the effect of Injinchunggan-tang on cytokine secretion. Methods : In vitro model of HCV infection in MOLT 4 cell was used. The effect of Injinchunggan-tang on the attachment of HCV on MOLT 4 cell was studied by PCR method. The change of cytokine secretion according to Injinchunggan-tang treatment was investigated by ELISA. Results : Injinchunggan-tang inhibited the attachment of HCV on MOLT 4 in the concentration of $10-2{\mu\textrm{g}}/\mu\textrm{\ell}$ and $10-1{\mu\textrm{g}}/\mu\textrm{\ell}$. In cytokine assay, Injinchunggan-tang increased the secretion of IL-4 of mouse splenocytes and PBMC in 48 hour culture as well as the secretion of IL-12 of mouse splenocytes and PBMC in 48 hour culture, whereas it decreased the secretion of $IFN-{\gamma}$ of mouse splenocytes in 24 and 48 hour culture. Conclusion : The results of this study show that Injinchunggan-tang has an inhibitory effect on the attachment on HCV on Mo1t4 Cell, and that it increases the secretion of IL-4 and IL-12 of mouse splenocyte and PBMC.

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A New Spin Filter for High Density Culture and Ethanol Production by Saccharomyces cerevisiae

  • Moon, Hyun-Soo;Lim, Dong-Joon;Song, Gu-Young;Kang, Hyun-Ah;Kim, Seung-Wook;Kim, Ik-Hwan;Hong, Suk-In
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.406-410
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    • 2002
  • A new spin filter consisting of $50{\mu}m$ (nominal pore size) depth fitters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.

A Mathematical Model for the Whole Ripening Process of Cheddar Cheese (체다치즈의 숙성 전과정에 대한 수학식)

  • 김중균
    • KSBB Journal
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    • v.9 no.1
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    • pp.72-84
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    • 1994
  • A model to explain the observed kinetics in a whole process of Cheddar-cheese ripening has been developed. It includes growth and lysis of cells in the cheese matrix, cell-wall bound protelnases and intracellular dipeptidases that are released into cheese upon cell lysis, and the production of dipeptides and amino acids from casein in cheese. Model simulations have been conducted to figure out the crucial factors in the process of the cheese ripening. The influential factors have been found to be the cell numbers and the dipeptidase activity at the beginning of the cheese ripening, and the cell-lysis rate of cheese starters. The simulation results have also suggested the use of a mixed culture as well as the experimental screening for a more suitable organism as a cheese starter hence, the model shows how to accelerate the cheese ripening.

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Growth of Endothelial Cells on Microfabricated Silicon Nitride Membranes for an In Vitro Model of the Blood-brain Barrier

  • Harris, Sarina G.;Shuler, Michael L.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.4
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    • pp.246-251
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    • 2003
  • The blood-brain barrier (BBB) is composed of the brain capillaries, which are lined by endothelial cells displaying extremely tight intercellular junctions. Several attempts at creating an in vitro model of the BBB have been met with moderate success as brain capillary endothelial cells lose their barrier properties when isolated in cell culture. This may be due to a lack of recreation of the in vivo endothelial cellular environment in these models, including nearly constant contact with astrocyte foot processes. This work is motivated by the hypothesis that growing endothelial cells on one side of an ultra-thin, highly porous membrane and differentiating astrocyte or astrogliomal cells on the opposite side will lead to a higher degree of interaction between the two cell types and therefore to an improved model. Here we describe our initial efforts towards testing this hypothesis including a procedure for membrane fabrication and methods for culturing endothelial cells on these membranes. We have fabricated a 1 $\mu\textrm{m}$ thick, 2.0 $\mu\textrm{m}$ pore size, and 55% porous membrane with a very narrow pore size distribution from low-stress silicon nitride (SiN) utilizing techniques from the microelectronics industry. We have developed a base, acid, autoclave routine that prepares the membranes for cell culture both by cleaning residual fabrication chemicals from the surface and by increasing the hydrophilicity of the membranes (confirmed by contact angle measurements). Gelatin, fibronectin, and a 50/50 mixture of the two proteins were evaluated as potential basement membrane protein treatments prior to membrane cell seeding. All three treatments support adequate attachment and growth on the membranes compared to the control.

Embryonic Stem Cell-Preconditioned Microenvironment Effects on Epidermoid Carcinoma

  • Ryoo, Zae Young;Kim, Myoung Ok
    • Reproductive and Developmental Biology
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    • v.36 no.4
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    • pp.275-281
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    • 2012
  • Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change cell morphology and proliferation. This microenvironment induces cancer cell reprogramming and results in a change in cancer cell properties such as differentiation and migration. The cancer microenvironment affects cancer cell proliferation and growth. However, the mechanism has not been clarified yet. Using the ES-preconditioned 3-D microenvironment model, we provide evidence showing that the ES microenvironment inhibits proliferation and reduces oncogenic gene expression. But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and methylation on Oct4 promoter region. Furthermore, methylation of Nanog was increase on ES-preconditioned microenvironment and supports results that no difference on RNA expression levels. Taken together, these results demonstrated that in the ES-preconditioned 3-D microenvironment is a crucial role for cancer cell proliferation not senescence.

Brassinosteroids Accelerate the Rate of Cell Division in Isolated Petal Protoplasts of Petunia hybrida

  • Oh, Man-Ho
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.69-77
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    • 2003
  • Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been extensively studied. The effect of brassinolide on the kinetics and final division frequencies of regenerating petal protoplasts of Petunia hybrida Vilm v. Comanche was examined. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide not only reduced the time of first cell division by 4.5 days but also altered the final division frequencies after 10 days of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 9%. Under sub-optimal auxin conditions, 10-100 nM brassinolide accelerated the first cell division, but no significant increase in the 8-10 days final division frequencies. Isolated protoplasts may provide a useful model system for the investigation of the molecular mechanisms of brassinosteroid action on cell division and proliferation in higher plants.

Brassinosteroids Accelerate the Rate of Cell Division in Isolated Petal Protoplasts of Petunia hybrida

  • Oh, Man-Ho
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.63-67
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    • 2003
  • Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been extensively studied. The effect of brassinolide on the kinetics and final division frequencies of regenerating petal protoplasts of Petunia hybrida Vilm v. Comanche was examined. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide not only reduced the time of first cell division by 4.5 days but also altered the final division frequencies after 10 days of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 9%. Under sub-optimal auxin conditions, 10-100 nM brassinolide accelerated the first cell division, but no significant increase in the 8-10 days final division frequencies. Isolated protoplasts may provide a useful model system for the investigation of the molecular mechanisms of brassinosteroid action on cell division and proliferation in higher plants.

Factors Affecting Primary Culture of Nuclear Transfer Blastocysts for Isolation of Embryonic Stem Cells in Miniature Pigs

  • Kim, Min-Jeong;Ahn, Kwang-Sung;Kim, Young-June;Shim, Ho-Sup
    • Reproductive and Developmental Biology
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    • v.33 no.3
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    • pp.133-137
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    • 2009
  • Pluripotent embryonic stem (ES) cells isolated from inner cell mass (ICM) of blastocyst-stage embryos are capable of differentiating into various cell lineages and demonstrate germ-line transmission in experimentally produced chimeras. These cells have a great potential as tools for transgenic animal production, screening of newly-developed drugs, and cell therapy. Miniature pigs, selectively bred pigs for small size, offer several advantages over large breed pigs in biomedical research including human disease model and xenotransplantation. In the present study, factors affecting primary culture of somatic cell nuclear transfer blastocysts from miniature pigs for isolation of ES cells were investigated. Formation of primary colonies occurred only on STO cells in human ES medium. In contrast, no ICM outgrowth was observed on mouse embryonic fibroblasts (MEF) in porcine ES medium. Plating intact blastocysts and isolated ICM resulted in comparable attachment on feeder layer and primary colony formation. After subculture of ES-like colonies, two putative ES cell lines were isolated. Colonies of putative ES cells morphologically resembled murine ES cells. These cells were maintained in culture up to three passages, but lost by spontaneous differentiation. The present study demonstrates factors involved in the early stage of nuclear transfer ES cell isolation in miniature pigs. However, long-term maintenance and characterization of nuclear transfer ES cells in miniature pigs are remained to be done in further studies.