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A New Spin Filter for High Density Culture and Ethanol Production by Saccharomyces cerevisiae  

Moon, Hyun-Soo (Graduate School of Chemical Biotechnology, Korea University)
Lim, Dong-Joon (School of Chemical Engineering and Technology, Yeungnam University)
Song, Gu-Young (School of Chemical Engineering and Technology, Yeungnam University)
Kang, Hyun-Ah (Korea Research Institute of Bioscience and Biochnology)
Kim, Seung-Wook (Department of Chemical Engineering, Korea University)
Kim, Ik-Hwan (Graduate School of Chemical Biotechnology, Korea University)
Hong, Suk-In (Department of Chemical Engineering, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.3, 2002 , pp. 406-410 More about this Journal
Abstract
A new spin filter consisting of $50{\mu}m$ (nominal pore size) depth fitters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.
Keywords
High density culture; spin filter; centrifugal force; pivotal movement; yeast;
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