• Advances in Traditional Medicine
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• 제8권2호
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• pp.154-163
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• 2008

The methanol extract of stem barks of Careya arborea Roxb. (MECA) (Family- Myrtaceae) was evaluated for antitumor activity and antioxidant status against Ehrlich's Ascites Carcinoma (EAC) bearing Swiss albino mice. After 24 h of tumor inoculation the MECA was administered at the doses of 50, 100 and 200 mg/kg body weight/mice/day for 14 days. After the last dose and 18 h fasting mice were sacrificed. The effect of MECA on the growth of transplantable murine tumor, life span of EAC bearing hosts, hematological profiles, serum and liver biochemical parameters were estimated. The MECA showed significant (P < 0.01) decrease in ascites volume, packed cell volume and viable cell count and prolonged the life span of EAC tumor bearing mice. Hematological profiles reverted to more or less normal levels in extract treated mice. The MECA also produced protective effect by decreasing the activity of serum enzymes, bilirubin and increase the protein and uric acid levels. MECA significantly (P < 0.05) decreased the levels of lipid peroxidation, while significantly (P < 0.05) increased the levels of glutathione content, vitamin C, vitamin E, superoxide dismutase and catalase CAT. The results indicate that MECA exhibited significant antitumor and antioxidant activity in EAC bearing mice.

• 동의생리병리학회지
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• 제27권6호
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• pp.771-781
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• 2013

This study was performed to investigate the anti-photoaging effects of Persimmon leaf tea(PLT) in hairless mice(SKH-1) exposed to UVB irradiation. The animals were divided into non-treated group (normal, N) and UV-radiated groups. UV-radiated groups were divided into only UV-radiated group(control, C) and UV-radiated and PLT treated experimental groups[first extraction treated group(PLT-I), second extraction treated group(PLT-II), and third extraction treated group(PLT-III)]. Three PLT treated experimental groups of mice were treated with both oral administration(300 mg/Kg B.W./day) and topical application (100 ul of 2% conc./mouse/day) for 4 weeks. Anti-photoaging effects of Persimmon leaf were evaluated by anti oxidative reaction, stereomicroscopic and microscopic observations. The expression of photoaging skin related factors including mast cell tryptase, proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) was examined by immunohistochemical staining. Treatment of PLT-I, -II, -III prevented the wrinkle formation as well as epidermal hyperplasia, inflammatory cells, disruption of collagen in photoaged skin induced by UVB radiation. It also reduced the PCNA and VEGF expression in the UVB irradiated dorsal skin. Furthermore, it significantly decreased the number of mast cells in the UVB irradiated dermis(p<0.05 and p<0.01). On the effects of oxidative stress and antioxidant function on the treatment with water extract from Persimmon leaf tea(PLT), the activity of superoxide dismutase(SOD) was significantly increased in PLT-III group(p<0.05), and catalase(CAT) was significantly increased in PLT-I and PLT-III groups(p<0.05), and PLT-II group(p<0.001). These extracts showed relatively antioxidant activity and protective effect on UVB-induced oxidative stress in hairless mice(SKH-1). Our results suggest that Persimmon leaf tea may serve as an useful radical scavenging antioxidant and anti-photoaging skin agents in the UVB irradiated skin.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.515-521
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• 2019

본 연구는 고지방 식이로 유발된 비만 모델의 지질 및 항산화 관련 효소의 대사에 대하여 와송 열수 추출물의 섭취를 통한 효과를 확인하였다. 정상군을 제외하고 나머지 군은 8주 동안 고지방식이를 공급하여 비만 상태로 유도하였으며, 9주에서 14주까지 고지방식이에 와송 열수 추출물이 2.5%, 5% 첨가된 식이를 자유 급식하였다. 그 결과, 고지방 식이군 대비 와송 추출물 섭취군에서 간과 폐의 무게가 감소되었으며, H&E 염색상에서도 lipid droplet이 현저하게 감소되는 것을 관찰할 수 있었다. 또한, 간조직내의 총 콜레스테롤과 중성지방과 같은 지질 함량이 유의성 있게 줄어들었다. 와송 추출물의 항산화제로서의 능력을 확인한 결과 DPPH 라디컬 소거능의 활성을 증가하였고, ROS 형성량은 탁월하게 감소되었다. 산화스트레스를 감소시키는 SOD와 CAT와 같은 항산화 효소의 활성이 와송 추출물의 고농도 섭취군에서 정상군에 근접하게 회복되었다. 결론적으로 와송 추출물의 섭취는 간내 지질대사를 조절하고 항산화 효소를 활성화시켜 비만 치료를 위한 생리활성물질로 유용하게 활용 가능할 것으로 생각된다.

• 환경생물
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• 제24권2호
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• pp.119-125
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• 2006

본 연구에서는 붕어를 이용하여 탁도 변화에 따른 여러 조직의 항산화효소 활성을 조사하였다. 탁도 50, 100, 150 NTU에서 사육기간에 따른 붕어 조직의 항산화효소 활성의 변화는 50과 100 NTU에서는 대조구에 비해 큰 변화가 없었으며, 고탁도인 150 NTU에서 비교적 두드러진 차이를 나타냈다. 붕어 조직의 항산화능을 DPPH 소거 활성도에서 보면 아가미, 간 조직에서 높은 활성을 보여 고탁도의 사육 조건에서 장기간 사육될 때 비효소적 항산화작용이 일어나는 것으로 생각된다. 효소적 항산화작용은 50, 100, 150NTU로 탁도가 높아짐에 따라 항산화효소의 활성이 증가하였으며 SOD, CAT및 GST의 활성은 표피를 제외한 모든 조직에서 사육 후 20일까지는 증가한 후 사육 후기로 갈수록 감소하는 경향을 나타냈다. 그러나 GR과 GPX의 활성은 거의 모든 조직에서 활성이 매우 낮으므로 어류의 항산화과정에서 이들 효소의 역할은 매우 적을 것으로 생각된다.

• Nutrition Research and Practice
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• 제3권2호
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• pp.156-161
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• 2009

Hyperglycemia in the diabetic state increases oxidative stress and antioxidant therapy can be strongly correlated with decreased risks for diabetic complications. The purpose of this study is to determine antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes. The antioxidant activity of garlic and aged black garlic was measured as the activity in scavenging free radicals by the trolox equivalent antioxidant capacity (TEAC) assay. Three week-old db/db mice were fed AIN-93G diet or diet containing 5% freeze-dried garlic or aged black garlic for 7 weeks after 1 week of adaptation. Hepatic levels of lipid peroxides and activities of antioxidant enzymes were measured. TEAC values of garlic and aged black garlic were $13.3{\pm}0.5$ and $59.2{\pm}0.8{\mu}mol/g$ wet weight, respectively. Consumption of aged black garlic significantly decreased hepatic thiobarbituric acid reactive substances (TBARS) level compared with the garlic group which showed lower TBARS level than control group (p<0.05). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of garlic and aged black garlic group were significantly elevated compared to the control group. Catalase (CAT) activity of aged black garlic group was increased compared with the control group. These results show that aged black garlic exerts stronger antioxidant activity than garlic in vitro and in vivo, suggesting garlic and aged black garlic, to a greater extent, could be useful in preventing diabetic complications.

• Nutrition Research and Practice
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• 제14권4호
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• pp.334-351
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• 2020

BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson's trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

• 대한본초학회지
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• 제30권4호
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• pp.81-88
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• 2015

Objectives : The aim of present study was to evaluate the beneficial effect of Scutellariae Radix (SR) and Scutellariae Radix EtOH-heated at 200℃ (SR200) using lipopolysaccharide (LPS) treated intestine of mice.Methods : Extract of SR and SR200 were orally administrated. Their effects were compared with vehicletreated LPS and normal groups. Subsequently, we measured reactive oxygen species (ROS) and nitric oxide in the serum and western blotting in the intestine.Results : The average weight in LPS treated (Vehicle) group was lowered significantly compare to that in non-treated normal group and this weight loss in the vehicle group was effectively prevented by the administration of SR and SR200 respectively. The increased oxidative stress biomarker levels such as reactive oxygen species (ROS) and nitric oxide (NO) in the serum was markedly decreased by treated with SR200. The decreased levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) induced by LPS injection were significantly restored by both SR and SR200 treatment. Moreover, increased inflammatory mediators and cytokines such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) in the LPS treated vehicle mice were significantly decreased through down-regulation c-JUN through reduction of oxidative stress.Conclusions : SR and SR200 could have benefit effect through down-regulation of abnormal oxidative stress in LPS induced intestine injury mice. Moreover, The anti-inflammatory activity of SR200 extract was better than SR extract in the LPS induced intestine injury mice.

• KSBB Journal
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• 제26권1호
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• pp.27-32
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• 2011

For the development of functional food and cosmetics using hot water extract of Muraenesox cinereus's skin, contents of vitamin, amino acid and element, and antioxidant activity were investigated. The results are shown as follows: among vitamins, A(0.21mg/100 g), C (78.12mg/100 g), $D_3$ (0.03 mg/100 g), E (1.97 mg/100 g) and Niacin (2.53mg/100 g) were detected, respectively.Mineral contents were an order of K > P > Na > Mg > Ca > Fe and Zn. Contents of total amino acids were an order of Pro > Gly > Arg > Glu > Phe > Ala. Especially, the sum of total amino acids was 27.17 mg/100 mL, which was about 4.0 fold higher than that of free amino acid. DPPH radical scavenging activity of hot water extract of M. cinereus's skin at 25 mg/mL was 63.5% and did not increase at above 50 mg/mL. Activities of antioxidant enzymes in the liver of ethanol-treated rats using hot water extract of M. cinereus's skin were investigated. Compared to control group, activities of ADH and GSH-px were decreased. In the case of CAT and SOD activity, they were increased. These results showed that the hot water extract of M. cinereus's skin can be applied to raw macterial for functional food and cosmetics.

• 한국식품영양과학회지
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• 제33권4호
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• pp.653-658
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• 2004

Wistar계 흰쥐에게 두릅열수추출물을 7주간 급여하였으며 당뇨유발은 희생 2주전 streptozotocin을 투여하여 혈당이 300 mg/dL 이상인 흰쥐를 대상으로 유해 활성 산소 대사계에 미치는 영향을 구명하였다. 간조직 중의 유해 활성산소 생성계인 P-450 함량과 XO 및 AD 활성은 정상군에 비하여 당뇨대조군에서 유의적으로 감소된 반면, AH 활성은 증가 되었다. 두릅열수추출물 급여로 이들 유해 활성 생성계 효소 활성 변화가 완화되는 것으로 나타났다. 또한 SOD, CAT, GSH-Px 및 GST, GR과 같은 유해 활성산소 제거계와 G6PD 효소들은 당뇨유발로 유의적인 증가를 보였으나, 두릅열수추출물 급여군에서는 이들 효소 활성이 낮았다. 당뇨유발로 항산화물질인 글루타티온 함량은 유의적으로 감소된 반면 지질과산화물 생성은 유의적으로 증가되었다. 그러나 두릅열수추출물 급여는 당뇨대조군의 이들 함량 변화를 유의적으로 억제하는 것으로 나타났다. 이상의 결과에서 두릅열수추출물 급여는 STZ 투여로 인한 유해 활성산소 대사계 변화를 완화시킬 뿐만 아니라 지질과산화물 생성을 억제함으로써 산화적 스트레스로 인한 당뇨합병을 예방할 수 있을 것으로 사료된다.

• 한국식품영양과학회지
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• 제36권4호
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• pp.411-418
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• 2007

솔잎 열수추출물이 카드뮴으로 유도한 흰쥐의 산화적 손상에 미치는 영향을 살펴보고자 체중 kg당 5mg의 카드뮴$(CdCl_2)$을 매주 1회 경구투여하였다. 솔잎은 매일 일정시각에 체중 kg당 1.26g수준이 되도록 4주간 경구투여 사육한 결과 카드뮴과 솔잎 열수추출은 체중에는 영향을 미치지 않았으며 솔잎 열수추출물은 식이섭취량을 감소시켰다. 체중 100g당 간조직 무게는 카드뮴 대조군이 정상군에 비하여 유의적으로 증가하였으며 솔잎 열수추출물은 간조직 무게를 감소시키는 경향이었다 혈장 중의 AST와 ALT활성은 카드뮴 투여에 의해 유의적으로 증가되었으나 솔잎 열수추출물에 의해 ALT활성이 카드뮴 대조군에 비하여 유의적으로 감소되었다. 혈장 알부민 함량은 실험군간의 차이가 없었으나 크레아티닌 함량은 솔잎 열수추출물 급여시 낮았다. 카드뮴 투여시 간조직의 CYP함량은 유의적으로 감소되었으며 솔잎 열수추출물에 의한 영향은 없었다. 한편 XO와 ADH활성은 솔잎 열수추출물군이 정상군과 카드뮴 대조군에 비하여 유의적으로 높았다. 또한 카드뮴 투여에 인해 유의적으로 높아진 SOD, MAO, CAT 및 GSH-Px 활성도는 솔잎 열수추출물에 의해 정상수준으로 회복되었다. 간조직 중의 글루타티온 함량은 솔잎 열수추출물 급여에 의한 차이가 발견되지 않았으나 MDA함량은 솔잎 열수추출물 급여군에서 카드뮴 대조군에 비하여 유의적인 감소를 보임으로써 카드뮴에 의해 유도된 지질과산화가 솔잎 열수추출물 급여로 개선될 수 있음을 제시한다.