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Effects of Persimmon leaf on the Photoaging Skin Improvement(1)  

Lee, Chang Hyun (College of Oriental Medicine, Woosuk University)
Kim, Nam Seok (Department of Food & Biotechnology, Woosuk University)
Choi, Dong Seong (Department of Food & Biotechnology, Woosuk University)
Oh, Mi Jin (Department of Food & Biotechnology, Woosuk University)
Ma, Sang Yong (Department of Alternative Medicine, Woosuk University)
Kim, Myoung Soon (Department of Alternative Medicine, Woosuk University)
Ryu, Seung Jeong (Dongsangmyunsaram farming cooperations)
Kwon, Jin (Department of Prosthetics and Orthotics, Korea National College of Welfare)
Shin, Hyun Jong (College of Oriental Medicine, Woosuk University)
Oh, Chan Ho (Department of Food & Biotechnology, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.27, no.6, 2013 , pp. 771-781 More about this Journal
Abstract
This study was performed to investigate the anti-photoaging effects of Persimmon leaf tea(PLT) in hairless mice(SKH-1) exposed to UVB irradiation. The animals were divided into non-treated group (normal, N) and UV-radiated groups. UV-radiated groups were divided into only UV-radiated group(control, C) and UV-radiated and PLT treated experimental groups[first extraction treated group(PLT-I), second extraction treated group(PLT-II), and third extraction treated group(PLT-III)]. Three PLT treated experimental groups of mice were treated with both oral administration(300 mg/Kg B.W./day) and topical application (100 ul of 2% conc./mouse/day) for 4 weeks. Anti-photoaging effects of Persimmon leaf were evaluated by anti oxidative reaction, stereomicroscopic and microscopic observations. The expression of photoaging skin related factors including mast cell tryptase, proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) was examined by immunohistochemical staining. Treatment of PLT-I, -II, -III prevented the wrinkle formation as well as epidermal hyperplasia, inflammatory cells, disruption of collagen in photoaged skin induced by UVB radiation. It also reduced the PCNA and VEGF expression in the UVB irradiated dorsal skin. Furthermore, it significantly decreased the number of mast cells in the UVB irradiated dermis(p<0.05 and p<0.01). On the effects of oxidative stress and antioxidant function on the treatment with water extract from Persimmon leaf tea(PLT), the activity of superoxide dismutase(SOD) was significantly increased in PLT-III group(p<0.05), and catalase(CAT) was significantly increased in PLT-I and PLT-III groups(p<0.05), and PLT-II group(p<0.001). These extracts showed relatively antioxidant activity and protective effect on UVB-induced oxidative stress in hairless mice(SKH-1). Our results suggest that Persimmon leaf tea may serve as an useful radical scavenging antioxidant and anti-photoaging skin agents in the UVB irradiated skin.
Keywords
Anti-photoaging; persimmon leaf; hairless mice(SKH-1); anti-oxidative reaction; immunohistochemistry;
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