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Spermiogenesis in the Korean long-Fingered Bat (Miniopterus schreibersi fuliginosus) (한국산 긴날개 박쥐(Miniopterus schreibersi fuliginosus)의 정자변태)

  • 손성원;이정훈;최병진;신화정
    • The Korean Journal of Zoology
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    • v.38 no.3
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    • pp.405-416
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    • 1995
  • The testis and the epididymis of sexually mature male bats were examined to investigate the process of spermiogenesis of Korean long-fingered bat (Miniopterus schreifersi fulignosus) using electron microscope. The ultrastructural findings were analysed on the basis of Lee's method (1992). Especially, we focused on the acrosome formation. The results are as follows: The spermiogenesis of the Korean long-fingered bat can be divided into ten phases on the basis of ultrastructural differentiation; three "Golgi" phases of early, mid and late stages, two "cap" and two "acrosome" phases respectively composed of early and late phases, one "maturation phase and two "spermiation" phases of early and late phases. The axoneme of sperm in the cauda epididymis is composed of nine outer dense fiber and a central singlet. The number 1, 5, 6, and 9 outer dense fibers are larger than others. In the Golgi phases, small vesicles are separated from Golgi vesicles and then appear to fuse into a large vesicle, and finally it contacd with the outerside of the nucleus. It suggests that proacrosomal material could be made in the cytoplasm before the Golgi vesicle formation and then it could be transferred into the Golgi vesicle and condensed more and more, and finally form acrosome, just as Lee;s suggestion (1992).m acrosome, just as Lee;s suggestion (1992).

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A Study on the Habitat Environment and Mutualism with Ants of Genus Melampyrum - focusing on M. roseum, M. setaceum var. nakaianum and M. roseum var. ovalifolium in Kangwondo - (며느리밥풀속 서식지 특성 및 개미와의 공생관계에 관한 연구 - 강원도의 꽃며느리밥풀, 새며느리밥풀 및 알며느리밥풀을 중심으로 -)

  • Lee, Jae-Hyeon;Lyu, Dong-Pyo;Kim, Gab-Tae
    • Korean Journal of Environment and Ecology
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    • v.30 no.2
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    • pp.139-145
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    • 2016
  • To search for habitat characteristics of myrmecochory Melampyrum species, diaspores and related ant species, habitat characteristics, collecting fruits and diaspores of three Melampyrum species, cafeteria experiments, and the related ant species were studied from May 2012 to November 2014. The habitat of Melampyrum species was south-facing open forest edge with slightly acidic soil and relatively shallow soiil layers. Major plants coexisting with Melampyrum species were four woody species including Quercus mongolica and four herbaceous species including Carex siderosticta. These Plants might be host plants of the Melampyrum species. The shape of the diaspore was an oval seed attached to the white cap on one side. Significant differences is proved between three plant species in weight of diaspores, seeds and and elaiosome ratios. Mean values of diaspores weight showed 10.6mg, 8.0mg and 7.2mg in M. setaceum var. nakaianum, M. roseum var. ovalifolium and M. roseum, respectively. The heaviest elaiosome is M. roseum var. ovalifolium with 1.2mg, M. setaceum var. nakaianum has 1.0mg and the lightest elaiosome is M. roseum with 0.8mg. Major ant species frequently transporting the diaspores of Melampyrum species are Myrmicinae four species; Myrmica kotokui, M. excelsa, Myrmecina nipponica and Aphaenogaster japonica. Further studies on Melampyrum species, hemiparasite annual myrmecochorous plant, and related ant species should be needed.

Joint Electromagnetic Inversion with Structure Constraints Using Full-waveform Inversion Result (완전파형역산결과를 구조적 제약 조건으로 이용한 고해상도 전자탐사 복합역산 알고리듬 개발)

  • Jeong, Soocheol;Seol, Soon Jee;Byun, Joongmoo
    • Geophysics and Geophysical Exploration
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    • v.17 no.4
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    • pp.187-201
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    • 2014
  • Compared with the separated inversion of electromagnetic (EM) and seismic data, a joint inversion using both EM and seismic data reduces the uncertainty and gives the opportunity to use the advantage of each data. Seismic fullwaveform inversion allows velocity information with high resolution in complicated subsurface. However, it is an indirect survey which finds the structure containing oil and gas. On the other hand, marine controlled-source EM (mCSEM) inversion can directly indicate the oil and gas using different EM properties of hydrocarbon with marine sediments and cap rocks whereas it has poor resolution than seismic method. In this paper, we have developed a joint EM inversion algorithm using a cross-gradient technique. P-wave velocity structure obtained by full-waveform inversion using plane wave encoding is used as structure constraints to calculate the cross-gradient term in the joint inversion. When the jointinversion algorithm is applied to the synthetic data which are simulated for subsea reservoir exploration, images have been significantly improved over those obtained from separate EM inversion. The results indicate that the developed joint inversion scheme can be applied for detecting reservoir and calculating the accurate oil and gas reserves.

A Study about the Real Estate' Policy Impact on house prices (Focusing on the time series analysis and regression) (부동산정책이 주택가격에 미치는 영향에 관한 연구 (시계열분석과 회귀분석 중심으로))

  • Ko, Pill-Song;Park, Chang-Soo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.5 no.2
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    • pp.205-213
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    • 2010
  • This study was to analyze the past regime's real estate policy and the time-series data on real estate price index from 1986 to 2009 in 24 years. Also, the real estate index and macroeconomic variables, the impact on house price index variable conducted to regression analysis and to analyze whether and how much is affected. Analyzed as follows: First, Korea's real estate policy was the post-policy and the past regime's real estate policy was inconsistent with each other. Second, in the normal phase whenever real estate issues, the measures of the strengthening regulation and of the economic recovery were only to repeat periodically. Third, the timing and means of policy enforcement was an inappropriate and Real estate market was getting worse at the time whenever a real estate policies performed. Fourth, The apartments prices index of the housing types rose the highest and were the most popular for 24 years. Increase or decrease the amount of the price index for apartments, Roh Tae-woo(65.0%) - Kim Dae-jung (42.5%) - Roh Moo-hyun (32.8%) were in order. Fifth, the results of the regression analysis carried out: The impact on housing prices among independent variables were followed by Cap Construction- one per capita income - Housing consumer price index - Accompanying Composite Index - Trailing Composite Index - Home subscription Subscriber account - Leading Composite Index.

Research Priorities to Support Mandatory Implementation of a Total Pollutant Load Management System (TPLMS) in the Han River Basin (한강수계 의무적 수질오염총량관리제시행지원을 위한 조사·연구의 우선순위 설정)

  • Lee, Chang-Hee;Lee, Bum-Yeon;Lee, Su-Woong
    • Journal of Environmental Policy
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    • v.8 no.4
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    • pp.25-36
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    • 2009
  • The Total Pollutant Load Management System(TPLMS) in the Han River basin is being changed from a voluntary to a mandatory system. Accordingly, this study suggests directions and priorities for research that can support implementation of TPLMS through an objective approach that deploys gap analysis and analytic hierarchy processes (AHP). Gap analysis indicated that TPLMS in Korea is still focused on compliance with regulations, and that implementation of TPLMS is still in its early stage. Improvements are thus needed in flexibility and effectiveness, including introduction of emissions rights trading, and upgrading to a renewable emissions permit system. The AHP study indicated that R&D will need to proceed in parallel in multiple areas to improve systems and resolve scientific uncertainties. Balanced R&D will be needed in both the institutional and technical groups. Subgroup analysis indicated that developing a reasonable process to establish water quality management targets is of the highest priority in the institutional group. In the technical group, higher priority will need to be given to improving model reliability and developing innovative pollution load reduction technologies.

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Cholinesterase Activity in the Dental Epithelium of Hamsters During Tooth Development

  • Yang, Jin-Young;Kim, Tak-Heun;Lee, Ju-Yeon;Jiang, Eun-Ha;Bae, Young;Cho, Eui-Sic
    • International Journal of Oral Biology
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    • v.35 no.4
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    • pp.169-175
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    • 2010
  • Cholinesterase (ChE) is one of the most ubiquitous enzymes and in addition to its well characterized catalytic function, the morphogenetic involvement of ChE has also been demonstrated in neuronal tissues and in non-neuronal tissues such as bone and cartilage. We have previously reported that during mouse tooth development, acetylcholinesterase (AChE) activity is dynamically localized in the dental epithelium and its derivatives whereas butyrylcholinesterase (BuChE) activity is localized in the dental follicles. To test the functional conservation of ChE in tooth morphogenesis among different species, we performed cholinesterase histochemistry following the use of specific inhibitors of developing molar and incisors in the hamster from embryonic day 11 (E11) to postnatal day 1 (P1). In the developing molar in hamster, the localization of ChE activity was found to be very similar to that of the mouse. At the bud stage, no ChE activity was found in the tooth buds, but was first detectable in the dental epithelium and dental follicles at the cap and bell stages. AChE activity was found to be principally localized in the dental epithelium whereas BuChE activity was observed in the dental follicle. In contrast to the ChE activity in the molars, BuChE activity was specifically observed in the secretory ameloblasts of the incisors, whilst no AChE activity was found in the dental epithelium of incisors. The subtype and localization of ChE activity in the dental epithelium of the incisor thus differed from those of the molar in hamster. In addition, these patterns also differed from the ChE activity in the mouse incisor. These results strongly suggest that ChE may play roles in the differentiation of the dental epithelium and dental follicle in hamster, and that morphogenetic subtypes of ChE may be variable among species and tooth types.

Expression of PDL-specific protein;PDLs22 on the developing mouse tooth and periodontium (발생중인 생쥐 치아 및 치주조직에서 치주인대-특이 단백질; PDLs22의 발현)

  • Park, Jung-Won;Park, Byung-Ki;Kim, Sang-Mok;Kim, Byung-Ock;Park, Joo-Cheol
    • Journal of Periodontal and Implant Science
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    • v.32 no.1
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    • pp.1-12
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    • 2002
  • The periodontal ligament(PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. PDL-specific protein;PDLs22 had been previously identified as a novel protein isolated from cultured human PDL fibroblasts using subtraction hybridization between human gingival fibroblasts and PDL fibroblasts. The aim of this study was to examine the expression pattern and tissue localization of PDLs22 protein in embryonic and various postnatal stages of developing mouse using immunohistochemical staining. Embryos (E18) and postnatal (P1, P4, P5, P15, P18) were decapitated and the heads were fixed overnight in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for $2{\sim}4$ weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at $6{\mu}m$ in thickness. Polyclonal antiserum raised against PDLs22 peptides, ISNKYLVKRQSRD, were made. The localization of PDLs22 in tissues was detected by polyclonal antibody against PDLs22 by means of immunohistochemical staining. The results were as follows; 1. Expression of PDLs22 protein was not detected in the tooth germ of bud and cap stage. 2. At the late bell stage and root formation stage, strong expression of PDLs22 protein was observed in developing tooth follicle, osteoblast-like cells, and subodontoblastic cells in the tooth pulp, but not in gingival fibroblasts, ameloblasts and odontoblasts of tooth germ 3. In erupted tooth, PDLs22 protein was intensely expressed in PDL and osteoblast-like cells of alveolar bone, but not in gingival fibroblasts, mature osteocytes and adjacent salivary glands. 4. In the developing alveolar bone and mid-palatal suture, expression of PDLs22 protein was seen in undifferentiated mesenchymal cells and osteoblast-like cells of developing mid-palatal suture, but not in mature osteocytes and chondrocytes. These results suggest that PDLs22 protein may play an important role in the differentiation of undifferentiated mesenchymal cells in the bone marrow and PDL cells, which can differentiate into multiple cell types including osteoblasts, cementoblasts, and PDL fibroblasts. However, more researches should be performed to gain a better understanding of the exact function of PDLs22 protein which related to the PDL cell differentiation.

What is the effect of initial implant position on the crestal bone level in flap and flapless technique during healing period?

  • Al-Juboori, Mohammed Jasim;Ab Rahman, Shaifulizan;Hassan, Akram;Ismail, Ikmal Hisham Bin;Tawfiq, Omar Farouq
    • Journal of Periodontal and Implant Science
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    • v.43 no.4
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    • pp.153-159
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    • 2013
  • Purpose: The level of the implant above the marginal bone and flap design have an effect on the bone resorption during the healing period. The aim of this study is to detect the relationship between the level of the implant at the implant placement and the bone level at the healing period in the mesial and distal side of implants placed with flapless (FL) and full-thickness flap (FT) methods. Methods: Twenty-two nonsubmerged implants were placed with the FL and FT technique. Periapical radiographs were taken of the patient at implant placement, and at 6 and 12 weeks. By using computer software, bone level measurements were taken from the shoulder of the healing cap to the first bone implant contact in the mesial and distal side of the implant surface. Results: At 6 weeks, the correlation between the crestal bone level at the implant placement and crestal bone level of the FT mesially was significant (Pearson correlation coefficient=0.675, P<0.023). At 12 weeks, in the FT mesially, the correlation was nonsignificant (Spearman correlation coefficient=0.297, P<0.346). At 6 weeks in the FT distally, the correlation was nonsignificant (Pearson correlation coefficient=0.512, P<0.107). At 12 weeks in the FT distally, the correlation was significant (Spearman correlation coefficient=0.730, P<0.011). At 6 weeks in the FL mesially, the correlation was nonsignificant (Spearman correlation coefficient=0.083, P<0.809). At 12 weeks in the FL mesially, the correlation was nonsignificant (Spearman correlation coefficient= 0.062, P<0.856). At 6 weeks in the FL distally, the correlation was nonsignificant (Spearman correlation coefficient=0.197, P<0.562). At 12 weeks in the FL distally, the correlation was significant (Pearson correlation coefficient=0.692, P<0.018). Conclusions: A larger sample size is recommended to verify the conclusions in this preliminary study. The bone level during the healing period in the FT was more positively correlated with the implant level at implant placement than in the FL.

Horizontal Bearing Behavior of Group Suction Piles by Numerical Analysis (수치해석을 이용한 그룹형 석션파일의 수평방향 지지거동 분석)

  • Lee, Ju-Hyung;Lee, Si-Hoon;Kim, Sung-Ryul
    • Journal of the Korean Geotechnical Society
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    • v.29 no.11
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    • pp.119-127
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    • 2013
  • Recently, several researches on the development of new economical foundation types have been performed to support floating structures as many offshore structures have been constructed. This study focused on the evaluation of bearing capacity of group suction piles, which are connected by a concrete pile cap. The offshore floating structures are mainly subjected to horizontal loading, so the horizontal bearing capacities of the group suction piles were analyzed by performing 3-dimensional finite element analyses. The group suction piles are expected to behave as a rigid pile due to its shallow embedded depth. Therefore, the detailed soil modeling was necessary to simulate the bearing behavior of soils under low confining pressure. The modulus and the strength of soils were modelled to increase with effective confining pressure in soils. For the parametric study, the center-to-center spacing between piles was varied and two soil types of clay and sands were applied. The analyses results showed that the yielding load of the group pile increased with the increase of the pile spacing and the yielding load of the group piles with 5D spacing was about 3 times larger than that of the single pile with free rotation.

Comparison of Pipeline and Clamshell Capping Technologies for the Remediation of Contaminated Marine Sediments (해양 오염퇴적물 정화를 위한 원통관과 클램쉘을 이용한 피복 기술의 비교)

  • Kang, Ku;Hong, Seong-Gu;Kim, Young-Kee;Park, Seong-Jik
    • Journal of Navigation and Port Research
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    • v.41 no.4
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    • pp.195-206
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    • 2017
  • In situ capping technology for marine sediment pollution control has never been applied in South Korea. In this study a pilot project for the capping was carried out in Busan N Harbor. Pipeline and clamshell capping technologies were implemented for the pollution control. Changes of capping shapes, sediment contamination, and the time and costs required for the two constructions were compared. Both the pipeline and clamshell technologies were found to satisfy the target thickness of 50 cm on average. However, the pipeline method did not operate sensitively in terms of change of the sea floor topography, resulting in an uneven shape and a thickness. Organic carbon and ignition loss quite decreased after the pipeline or the clamshell capping while pH showed no significant change. Organic and residual fraction of Cd, Ni, and Zn in the sediments appeared to decrease after all cappings. The pipeline method took a construction time four times as much as the clamshell method. The clamshell method was demonstrated to reduce the construction cost by about 40% compared with the pipeline method. However, a monitoring for all the parameters needs to be conducted at least two years in order to better evaluate an efficiency of the pollution control by these capping constructions.