• Journal of Animal Environmental Science
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• 제18권3호
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• pp.229-234
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• 2012

Disinfecting contaminated swine manure with FMD (Foot-and-Mouth Disease) and pathogenic viruses is very important for maintaining sanitized environment. However, very few research reported on this subject, especially post-disinfection to utilize the wastes as a renewable resource. This research is carried out to obtain basic information for chemical treatment in FMD SOP (Standard Operating Procedure, Korea) of contaminated swine manure. Using lime, sodium hydroxide, citric acid and hydrochloric acid, described in FMD SOP, the effects of chemical treatments on livestock manure were compared in this paper. Four combinations of alkali-acid treatments and four kinds of acid-alkali combinations were tested to find out the effective method. Total coliform bacteria in contaminated swine manure, $1.6{\times}10^4$ CFU/100 ml, decreased to the range of 1/1000~1/100 in all treatments. Some specific disinfectants increases BOD (Biochemical Oxygen Demand) and EC(Electric Conductivity), especially, alkaline treatments increases ammonia level than acid treatments. These findings suggest that the treatment methods should be considered as an important environmental factor in post-disinfection of contaminated animal manure with pathogenic microorganisms.

• Applied Microscopy
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• 제24권4호
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• pp.61-77
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• 1994

The calcium-binding proteins (CaBP), parvalbumin (PV) and calbindin-D 28K (calbindin) are particularly abundant and specific in their distribution, and present in different subsets of neurons in many brain regions. Although their physiological roles in the neurons have not been elucidated, they are valuable markers of neuronal subpopulations for anatomical and developmental studies. This study is designed to characterize dorsal lateral geniculate nucleus (dLGN) neurons and axon terminals in terms of differential expression of immunoreactivity (IR) for two well-known CaBPs, PV and calbindin. The experiments were carried out on 6 adult monkeys. Monkeys were perfused under deep Nembutal anesthesia with 2% paraformaldehyde and 0.2% glutaraldehyde in 0.1M phosphate buffer. After removal, the brains were postfixed for 6-8 hr in 2% paraformaldehyde at $4^{\circ}C$ and infiltrated with 30% sucrose at $4^{\circ}C$. Thereafter, they were frozen in dry ice. Serial sections of the thalamus, at $20{\mu}m$, were made in the frontal plane with a sliding microtome. The sections were stained for PV and calbindin with indirect immunocytochemical methods. For electron microscopy, after infiltration with 30% sucrose the blocks of thalamus were serially sectioned at $50{\mu}m$ with a Vibratome in the coronal plane and stained immediately by indirect ABC methods without Triton X-100 in incubation medium. Stained sections were postfixed in 0.2% osmium tetroxide, dehydrated and flat-embedded in Spurr resin. The block was then trimmed to contain only a selected lamina or interlaminar space. The dLGN proper showed strong PV IR in fibers in all laminae and interlaminar zones. Particularly dense staining was noted in layers 1 and 2 that contain many stained fibers from optic tract. Neuronal cell body stained with PV was concentrated only in the laminae. In these laminae staining was moderate in cell bodies of all large and medium-sized neurons, and was strong in cell bodies of some small neurons together with their processes. Calbindin IR was marked in the neuronal cell body and neuropil in the S layers and interlaminar zones whereas moderate in the neuropil throughout the nucleus. Regional difference in distribution of PV and calbindin IR cell is distinct; the former is only in the laminae and the latter in both the S layer and interlaminar space. The CaBP-IR elements were confined to about $10{\mu}m$ in depth of Vibratome section. The IR product for CaBP was mainly associated with synaptic vesicle, pre- and post-synaptic membrane, and outer mitochondrial membrane and along microtubule. PV-IR was noted in various neuronal elements such as neuronal soma, dendrite, RLP, F, PSD and some myelinated or unmyelinated axons, and was not seen in the RSD and glial cells. Only a few neuronal components in dLGN was IR for calbindin and its reaction product was less dense than that of PV, and scattered throughout cytoplasm of soma of some relay neurons, and was also persent in some dendrite, myelinated axons and RLP. The RSD, F, PSD and glial elements were always non-IR for calbindin. Calbindin labelled RLP were presynaptic to unlabeled dendrite or dendritic spine and PSD. Calbindin-labeled dendrite of various sizes were always postsynaptic to unlabeled RSD, RLP or F. From this study it is suggested that dLGN cells of different functional systems and their differential projection to the visual cortex can be distinguished by differential expression of PV and calbindin.

• Korean Journal of Poultry Science
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• 제30권1호
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• pp.17-28
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• 2003

A feeding trial was carried out to evaluate the effects of feeding Lactobacillus reuteri culture(LRC) on the performance, nutrients digestibility, intestinal microflora, serum metabolites, ammonia generation and litter dampness in broiler chicks. Four hundred eighty, one day old male chicks were fed into none, 0.1, 0.2 and 0.4% of LRC supplementation fer seven weeks. Basal diets excluded antibiotics contained ME 3,100, 3,100 kcal/kg, and CP 22.0, 20.0% for starter and grower, respectively. Weight gain of chicks fed LRC was significantly higher than no supplemental group in overall period(P<0.05). Feed intake was the highest in the 0.1% LRC, but not statistically different from other treatments. Feed conversion showed no significance among treatments. Viable Lactobacillus spp. number of chicks fed 0.2 and 0.4% LRC was significantly higher in cecum at seven weeks of age compared to the none(P<0.05). The tendency of anaerobes number was similar to Lactobacillus spp in ileum and cecum. Total number of E. coli and Salmonella were no difference in all treatments. In serum metabolites, feeding LRC increased triglyceride, and inorganic phosphorus, but no different total protein, albumin, total cholesterol, glucose, blood urea nitrogen and Ca. Nutrients digestibility improved significantly in 0.4% LRC compared to that of none(P<0.05). Fecal NH$_3$, gas generation was greatly decreased in the LRC supplemental groups(P<0.05). Moisture contents of bedding was also significantly decreased in LRC feeding group. It was concluded from the present study that feeding Lactobacillus reuteri culture improved the growth performance and nutrients digestibility of broiler chicks and minimize the fecal noxious gas emission.

• Korean Journal of Poultry Science
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• 제21권1호
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• pp.41-48
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• 1994

In order to get information for the improvement of feeding systems of egg-type layers, a dietary self-selection study was conducted. Corn diet, soybean meal diet, wheat bran diet, soybean meal+fish meal diet, and limestone diet were individually prepared for self-selection regime. Forty brown layers were offered either a control laying diet (C) or one of the 3 self-selection regime consisted of corn-soybean meal-limestone diet ($T_1$), corn-soybean meal-wheat bran-limestone diet ($T_2$) and corn-soybean meal+fish meal-wheat bran-limestone diet ($T_3$) for one wk each at 31 wk and 41 wk of age. In all dietary treatments, most of the eggs were laid from 0700 h 1300 h and hens consumed more feed, energy and protein on egg forming days than on non-forming days. On egg forming days, hens fed self-selection regime consumed less energy and protein than those fed control diet. On egg forming days, the consumption of feed, energy and protein increased continuously from 1000 h 2200 h in the control diet group but they decreased during the last 3 hours from 1900 h to 2200 h in self-selection regime groups. Up to 1600 h, calcium intake of the self-selection regime groups were much less than that of the control group but after that time, they increased markedly and were much more than that of the control group. The results of the study suggest that the practical self-selection regime for layers should be developed to meet the daily cyclic requirement for nutrients without consuming an excess energy and protein at certain times of the day.

• Korean Journal of Organic Agriculture
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• 제23권4호
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• pp.875-886
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• 2015

The upland soils (56 samples) from organic farms in Gyeonggi-do (12 sites), Gangwon-do (8 sites), Chungcheong-do (14 sites), Gyeongsang-do (4 sites), Jeollado (18 sites) in Korea were collected and their physical and chemical properties were analyzed by RDA's methods. In the results of physical property, the bulk density of soils averaged $1.14Mgm^{-3}$ (surface soil), $1.38Mgm^{-3}$ (subsoil), respectively. The porosity of them was 57%, 48%. Organically managed soil's (OS) bulk density was lower than conventional soil's but OS's porosity was a little higher than conventionally managed soil in surface soil. The depth of plough layer in organically managed soils was 21.2 cm indicating that the organic farming had good effect on soil physical property. In the results of chemical property, the surface soil pH was 6.9 and the contents of organic matter (OM) was $26gkg^{-1}$, available phosphate (Avail. $P_2O_5$) was $554mgkg^{-1}$, exchangeable calcium (Exch. Ca) was $8.9cmol_ckg^{-1}$, exchangeable potassium (Exch. K) was $0.89cmol_ckg^{-1}$, exchangeable magnesium (Exch. Mg) was $2.0cmol_ckg^{-1}$. The subsoil pH was 6.8 and the contents of OM was $21gkg^{-1}$, avail. $P_2O_5$ was $491mgkg^{-1}$, exch. Ca was $7.9cmol_ckg^{-1}$, exch. K was $0.68cmol_ckg^{-1}$, exch. Mg was $1.8cmol_ckg^{-1}$. The nutrient accumulation emerged in organic farming. Compared to the optimum nutrient range for the conventional upland soils, the exceed rate of pH, OM, available phosphate, and exchangeable Ca, K, and Mg was 79, 52, 64, 84, 66% and 55%, respectively, which mainly resulted from the over-application of lime materials or livestock manure compost. With these results it is suggested that organic farm need to reduce the use of inputs, which make soil alkalification or nutrient accumulation. More study on effects of inputs on lowering soil pH from alkalification could help organically managed soil to be improved.

• Journal of Preventive Medicine and Public Health
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• 제20권2호
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• pp.352-361
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• 1987

During the period of May 10 to June 6, 1987, we have conducted the survey about the number of the food daily taken and the intake of nutrition of the 83 pregnant women who have registered at Sudong Myun and Mikum-myun, Yangchu-gun which are demonstration area of the public health and medicine of the College of Medicine, Ewha Women's University, and of the 100 pregnant women who have been to the University Hospital attached to the above School. The result of the survery is as follows: 1) Among the above pregnant women, primigravida constituted 91 women and multigravida 92 women. As for the month of pregnancy, those who have been pregnant for ten months numbered 43 women (23.5%), which was the highest number. As for age, those who were 25 to 29 years old constituted 63.9% , which was the highest in percentage. 2) As for the level of education, those who graduated from high school constituted 49.2%, which was the highest in percentage. As for occuptation, those who were entirely in charge of household affairs as housewives were 89.6% , which comprised most of the jobs held by the above women. 3) As for the composition of a family in environmental food and nutrition, one generation was 68.3%, and two member family was 42.6% which was the highest. As for those taking rare of cooking and serving meals, housewives constituted 95.1%. 4) As for the economic situation of dietary life, those who were in the highest level (A level), were 47.0% in an urban area and 41.0% in a rural area, which were the highest in percentage. 5) The food which the above people selected after being pregnant was fruit not only in an urban area, but also in a rural area. And in terms of the percentage of fruit, an urban area and a rural area showed 44.0% and 54.2% respectively. As for milk, and urban area and a rural area showed 28.0% and 8.4% respecitively, which disclosed that the rate of a rural area was much lower than that of an urban area. 6) As for the number of food intake, 10-14 was 54.0% in an urban area, which was the highest. And 5-9 was 73.5% in a rural area, which was the highest. 7) As for the composition of food taken according to the number of food taken, meat showed the lowest rate in 5-9 group. (p<0.05) As the number of food taken increased, the rate of food taken (points) increased (2.4). Milk and milk products showed a low rate in 5-9 group and 15-19 group (p<0.05). Fruit intake showed a similar rate in all groups. As for vegetables, the rate of intake was high, as the number of food taken increased. Cereals showed a similar rate in all groups. Ans the points of intake was high. As for fat, the rate of intake was high, as the number of food taken increased. 8) In 5-9 group in the number of food intake, energy was 79.9% of RDA, protein was 64.9%, fat was 90.0%, carbohydrate was 81.6%, iron was 83.0%, calcium was 71.1%, vitamin A was 94.3%, vitamin $B_1$ was 77.8%, vitamin $B_2$ was 79.2%, and vitamin C was 88.2%, so that it showed the lowest sufficiency rate RDA. 15-19 group maintained the balance in energy and all nutrients nealy. In 20-24 group, fat was 142.4%, vitamin $B_2$ was 105.6% and vitamin C was 102.2%, so that it exceeded RDA.

• Korean Journal of Veterinary Service
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• 제17권3호
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• pp.227-246
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• 1994

Present investigations were undertaken in order to clarify the clinical status of Korean native cattle and dairy cattle(holstein). Blood, Feces and urine samples were collected from 247 Korean native cattle(222 adult and 25 calf), 224 dairy cattle(211 adult and 13 calf) at Chonbuk area and analyzed for clinical, serum chemical, hematological and urinary findings. In addition, we were examined the infection rate of Theileriosis, internal patasite and ring worm. The mean value for each component was calculated by statistical analysis using Excel computer program. From these investigations the following results were obtained. The mean values for RBC, PCV and etc in 433 adult cow(Korean native cattle and dairy cattle) were similar with other reports. But the mean values for MCHC of all species were lower than normal. Adult Korean native cattle and adult dairy cattle which showed hematologically normal levels were only 9.01% and 9.48%, respectively. Ahnormally high values for PCV, RBC and Hb were recorded in 7.66% of adult Korean native cattle, 20% of Korean native calf, 15.38% of dairy calf. Adult Korean native cattle and adult dairy cattle which showed hematologically anemia were 4.95% and 19.43% respectively, but all in Korean native calf and dairy calf showed normal values. Adult Korean native cattle, adult dairy cattle, Korean native calf and dairy calf which showed normal serum protein level were 84.0%, 90.8%, 50% and 44.4%, respectively. In present investigations, 50% of Korean native calf and 55.6% of dairy calf were decreased serum protein values under normal range. These abnormally decreased serum protein values mean the shortage of antibody, and these have a possibility to occrus to pneumonia and diarrhea. From these results, the economical loss caused by pneumonia was calculated as 124, 038, 833 won in the KNC and 742, 703, 430 won in the dairy calf rearing in Chonbuk area. Calculated economical loss caused by enteritis was 56, 658, 690 won in Korean native cattle. 476, 775, 799 won in dairy calf and the total loss amount to 533, 434, 488 won in Chonbuk area. Abnormally high values($21.7{\pm}4.0mg/dl) for serum calcium were recorded 49.6% in dairy cattle. The mean values of serum total cholesterol were $170.8{\pm}99.8mg/dl in Korean native cattle, $196.0{\pm}40.6mg/dl$ in Korean native calf, $202.9{\pm}86.0mg/dl$ in adult dairy cattle and $289.4{\pm}97.5mg/dl$ in dairy calf. The infection rate of internal parasite were as follows； adult Korean native cattle：21.2%, Korean native calf：80%, adult dairy cattle：67.8%. The estimated economical loss caused by internal parasites infection were 1, 120, 855, 837 won in Korean native calf, 4, 994, 959, 405 won in adult Korean native cattle, 3, 334, 751, 066 won in adult holstein, and the total loss amount to 9, 450, 566, 308 won. The infection rate of theileriosis were 1.4% in Korean native cattle and 6.6% in dairy cattle. The presumed Economical loss by T. sergenti infection were 154, 408, 482 won in Korean native cattle and 171, 577, 237 won in dairy cattle rearing at Chonbuk area. The infection rat of ringworm were 0.5% in Korean native cattle, 0.9% in adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12, 061, 532 won in Korean native cattle, 16, 895, 403 won in dairy cattle, and the total estimated loss amount to 28, 955, 935 won a year in Chonbuk area. The infection rate of ringworm were 0. 5% in Korean native cattle, 0.9% in adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12, 061, 532 won in Korean native cattle, 16, 895, 403 won in dairy cattle, and the total estimated loss amount to 28, 955, 935 won a year in Chonbuk area.

• Korean Journal of Veterinary Service
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• 제42권4호
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• pp.227-236
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• 2019

The complete blood count and serum biochemical parameters are essential tools for diagnosis and monitoring of physiological and disease conditions in dogs. These parameters may be affected by the breed and age of dogs. This study aimed to compare the changes of complete blood count and serum biochemical parameters according to the increasing age in 156 Sapsarees. In this study, the Sapsarees were clustered into ten age groups: four to six months, seven to nine months, ten to twelve months, one year, two years, three to four years, five to six years, seven to eight years, nine to ten years, and, eleven to twelve years old. In the result of the complete blood count, the total red blood cell count (P<0.001), hematocrit (P<0.001), and hemoglobin (P<0.001) values were significantly increased from 4~6 months to 2 years old Sapsarees. While the mean corpuscular volume (P<0.001) and mean corpuscular hemoglobin (P<0.001) values were significantly increased from 4~6 months to 3~4 years old. The mean corpuscular hemoglobin concentration was significantly (P=0.037) increased from 4~6 to 10~12-month-old Sapsarees. Total white cell count was significantly (P<0.001) decreased from 1 year to 9~10 years old. The result of differential white blood cell count showed that neutrophil count was significantly (P<0.001) increased from the age 4~6 months to 11~12 years old Sapsarees, whereas, lymphocyte (P<0.001), monocyte (P<0.001), and eosinophil (P=0.042) counts were significantly decreased from 7~9 months to 7~8 years, 4~6 months to 9~10 years, 3~4 to 5~6 years old, respectively. In the serum biochemistry result, the creatinine concentration was significantly (P<0.001) increased from 4~6 to 10~12 months, but significantly (P=0.006) decreased from 7~8 to 10~12 years old. Phosphate concentration was significantly (P<0.001) decreased from 3~4 to 9~10 years old, but significantly (P=0.021) increased from 9~10 to 11~12 years old. Calcium concentration significantly (P<0.001) decreased from 10~12 months to 11~12 years old. Total protein concentration significantly (P<0.001) increased from 4~6 months to 2 years old. While concentrations of albumin (P=0.004) and globulin (P<0.001) was significantly increased from 4~6 months to 3~4 years old, and from 4~6 months to 11~12 years old, respectively. The alkaline phosphate concentration was significantly (P<0.001) decreased from 4~6 months to 2 years old. We found that there are differences in the hematological parameters in relation with the increasing age in Sapsaree breed.

• Korean Journal of Soil Science and Fertilizer
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• 제24권4호
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• pp.254-259
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• 1991

The present study was carried out to investigate the effect of nitrogen(urea) application on the seasonal change in pH content of Bray No.1-P, organic matter, and exchangeable cations along the grassland soil profile and further to provide the fundamental information for optimizing the rate of fertilizer application to grassland. Soil samples were taken 20cm intervals upto 100cm soil depth in spring(May 26), summer(July 27), and autumn (October 18) of 1990. The obtained results are summerized as follow 1. In spring and summer, soil pH at 0-20cm soil depth of 28kg N/10a treatment was lowered by 0.7 and 1.0 in comparison with those the same soil depth of 0 kg N/10a treatment and the tendency in pH decrease during all season at the soil depth below 20cm was in the order of summer>spring>autumn. 2. Although Bray No.1-P content at the soil depth 0-20cm of 28kg N/10a treatment was lowered by 20ppm compared to 0 kg N/10a treatment in summer, there was no great difference in its content between 0kg N/10a and 28kg N/10a treatment at all soil depth in spring and summer. In autumn, its content at soil depth below 20cm of 28kg N/10a treatment was higher than that of in summer. 3. Organic matter content at 0-20cm soil depth of 0 and 28kg N/10a treatment in autumn was slightly lowered and on the whole there was very little change in it by soil depth and nitrogen application. 4. The calcium content of 0 and 28kg N/10a treatment was also slightly lowered by increase in soil depth and Mg and K contents were below 0.4 and 0.2 me/100g during all seasons, respectively. 5. Positive correlations were shown among the $NH_4-N$ content and pH, organic matter, Ca and Mg of 0 kg N/10a treatment, however, there was negative correlation ($r=-0.534^{*}$) between $NO_3-N$ content and pH of 28kg N/10a treatment in summer.

• Korean Journal of Soil Science and Fertilizer
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• 제25권1호
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• pp.38-43
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• 1992

A field experiment was conducted to find out the effect level of nitrogen fertilization on the yield and quality of hop (Hallertau variety) emphasis given to ${\alpha}$-acid content. Nitrogen was applied by the rates of 0, 12, 18, 24, 30 and 36kg per 10a. Results were summarized as follows. 1. Stages of cone-hair developing and coneripening were delayed with increment of nitrogen applied. Number of nodes and length of vine increased with nitrogen applid by up to 24kg N/10a. 2. Yield was the highest in the 24kg N/10a plot and cone weight had high significant correlations with yield without regard to the years of growth. No.of flower per side blanch and weight of 100 cones had a significant correlation with yield in the four-year old and five-year old Hop plants. 3. Nutrient content in Hop plants was in the order of CaO, total nitrogen, $K_2O$, MgO and $P_2O_5$. Content of total nitrogen, CaO and MgO was high in leaves while that of $P_2O_5$ and $K_2O$ was high in cone. Nitrogen, phosphorus and magnesium increased with increment of nitrogen fetilized while potassium and calcium decreased. 4. As for the distribution of nutrients in cone developing stage $SiO_2$ content was higher in lower part than in the upper part while $P_2O_5$ contetn was higher in upper part than in lower part of the plant. And content of nitrogen and potassium was higher in middle height than in upper and lower part. 5. The optimum levels of nitrogen application were 19.3kg for 2 years, 24.3kg for 3-year, 27.9kg for 4 years and 31.8kg/10a for 5-year old Hop, respectively. 6. Nitrogen uptake in cone showed a positive correlation with the content of ${\alpha}$-acid and ${\beta}$-acid in cone.