• 한국식품위생안전성학회지
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• 제38권6호
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• pp.537-543
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• 2023

본 연구에서는 대형마트, 편의점 및 온라인 마켓에서 수집한 조제커피 제품의 카페인 함량을 모니터링하고 표시사항의 적합성을 검토하고자 하였다. 카페인의 함량은 HPLC을 사용하여 정량 분석하였다. 그 결과, 콜드브루 제품의 카페인 함량은 0.31-1.04 mg이었으며 평균 0.55 mg으로 나타났다. 제품 1개당 평균 함량은 147.27 mg/bottle로 나타나, 일일 권장섭취량을 감안하였을 때 성인 1인당 2.7병을 섭취할 수 있다. 아메리카노 제품의 카페인 함량은 0.15-0.38 mg이었으며 평균 함량은 0.28 mg 수준으로 나타났다. 제품 1개당 평균 함량은 110.42 mg/bottle로 나타나, 일일 권장섭취량을 감안하였을 때 성인 1인당 3.6병을 섭취할 수 있는 것으로 나타났다. 디카페인 콜드브루 제품의 카페인 평균 함량은 5.14 mg/bottle로 나타났으며 일반 아메리카노 제품에 비해 약 95%이상 카페인이 제거되어 식품의약품안전처에서 제시한 디카페인 제품 기준에 충족하였다. 또한, 총 카페인 함량의 허용오차인 120%를 초과하지 않아 관리가 잘 이루어지고 있음을 확인하였다.

• 한국식품영양학회지
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• 제32권5호
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• pp.504-510
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• 2019

The purpose of this study was to compare the effects of soaking and ultrasonic extraction by observing the change of contents with extraction time of physicochemical properties (solid content, colorness, caffeine, chlorogenic acid, total polyphenols, DPPH, and ABTS). As a result of the analysis, solid content increased with longer extraction time and the whiteness tended to decrease with longer extraction time. Conversely, the extraction of functional materials showed a tendency to increase as the extraction time increased. Caffeine reached the maximum value after two hours soaking, but showed the same result as one hour for sonication. Chlorogenic acid did not show difference from the content of coffee extracted for one hour soaking only by sonication extraction for 30 minutes. The total polyphenols eluted with approximately two hours of soaking even after 30 minutes of sonication. DPPH and ABTS were insignificant in their concentrations, but their antioxidative effect was more than two hours of soaking with only 30 minutes of sonication. Sonication has a short time extraction from a functional aspect (caffeine content, chlorogenic acid, polyphenol content, and antioxidant capacity) and this experiment can provide basic data for the development of innovative recipes.

• Journal of Nutrition and Health
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• 제30권8호
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• pp.911-919
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• 1997

The purpose of this research was to investigate the effect of caffeine levels on calcium utilization in rats of different age and sex. Calcium utilization was compared in rats of different age(4 weeks and 10 months) and sex that were fed various levels of caffeine (0, 3.5, and 7mg/100g body weight) for 3 weeks. There was no significant difference in feed intake, serum calcium level, and ash content in tibia among the groups. Fecal calcium excretion was lower in young rats than in adults, Urinary calcium excretion significantly higher in the caffeine groups than that in the no-caffeine group. Daily retention and apparent absorbability of calcium in young rats were higher than those in adult rats. However, there was no significant difference among groups of different sex and caffeine levels. The results of this study suggest that caffeine consumption promotes urinary calcium excretion.

• Journal of Nutrition and Health
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• 제30권10호
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• pp.1160-1169
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• 1997

The purpose of this research was to investigate the effects of caffeine and calcium levels on calcium utilization in female rats of different ages. Calcium utilization was compared in female rats of different age( 4 weeks and 12 months) fed various levels of caffeine(0 and 7 mg/100g body weight) and calcium (50, 100 and 200% of requirement) for 3 weeks. Feed intake of the caffeine groups were lower than that of the no-caffeine groups. body weight gain was lowest in the high-caffeine and low-calcium group. Serum calcium levels of young rats were higher than those of adult rats. There were no significant differences in tibial calcium content among the caffeine and calcium -groups. Fecal calcium excretion increased as the level of dietary calcium was increased. Urinary calcium excretion increased as the levels of caffeine and dietary calcium were increased. With increasing levels of dietary calcium , daily calcium retention was accelerated, but apparent calcium absorbability was diminished. The results of this study suggest that caffeine consumption promotes urinary calcium excretion. However, increase in dietary calcium resulted in higher calcium retention . These findings indicate that high caffeine consumption may increase dietary calcium requirements. Therefore, it could be suggested that the supplementation of dietary calcium may counteract the negative effect of caffeine intake on calcium utilization.

• 한국식품영양과학회지
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• 제22권6호
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• pp.658-663
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• 1993

식이성 비타민 E의 급여수준이 카페인을 투여한 흰쥐의 지질대사에 미치는 영향을 검토하고자, 카페인을 무첨가 또는 0.3% 첨가시킨 식이에 비타민 E를 각각 세가지 급여수준(37.5, 750 및 1,500mg/kg diet)으로 사육기간을 5주와 10주로 하여 사육한 결고는 다음과 같다. 체중증가량, 식이섭취량 및 식이효율은 카페인 급여군에서 비타민 E 단독부여군에 비해 유의하게 감소하였고, 체중 100g당 간과 신장의 중량은 카페인 첨가군에서 유의한 증가를 보였다. 혈청과 간의 total lipid 함량은 카페인 첨가군에서 비타민 E 급여수준을 증가시킬수록 감소하였다. 혈청중 cholesterol함량은 카페인을 첨가하였을 때는 증가하였으나, 간장중 cholesterol의 함량은 비타민 E의 급여수준을 증가시킴에 따라 감소하는 경향이었다. Triglyceride함량은 혈청과 간 모두 카페인 첨가군에서 유의하게 감소하였으며, 그 감소의 정도는 비타민 E 급여수준이 증가할수록 크게 나타났다. 또 혈청 alanine aminotransferase와 aspartate aminotransferase의 활성은 모든 실험군에서 유의한 차이가 없었다.

• Journal of Nutrition and Health
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• 제41권3호
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• pp.216-223
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• 2008

본 연구는 0.03%의 카페인 함유 식이를 폐경모델인 난소절제쥐에서 6주간 섭취시켜 골밀도와 골무기질함량에 미치는 영향을 아래와 같이 요약하였다. 1) 체중증가량은 난소절제군이 Sham군에 비해 유의적으로 높았으며 각 군내에서 카페인 섭취에 따른 차이는 없었다. 2) 혈 중 칼슘 농도는 난소절제군내에서 카페인군이 대조군보다 유의적으로 낮게 나타났다. 3) 혈 중 ALP는 Sham군과 난소절제군 모두에서 카페인 군이 대조군보다 높은 경향을 나타내었다. 혈중 Osteocalcin은 Sham 군과 난소절제군, 그리고 각 군내에서 카페인 섭취여부에 따라 유의적인 차이를 나타내지 않았다. 4) 요 중 칼슘 및 인의 농도, 요 중 Deoxypyridinoline(DPD)와 crosslinks value는 Sham 군과 난소절제군, 그리고 각 군내에서 카페인 섭취여부에 따라 유의적인 차이를 나타내지 않았다. 5) 척추골밀도는 Sham군에 비해 난소절제군이 유의적으로 낮았고, 난소절제군내에서 카페인 첨가군과 대조군간에 차이가 없었다. 6) 대퇴골밀도와 대퇴 골무기질 함량은 Sham군과 난소 절제군 간의 차이는 없었고, 각 군내에서 식이에 따른 차이도 없었다. 따라서 카페인 0.03% caffeine 섭취는 난소절제쥐에서 6주간 섭취 시킨 경우 척추와 대퇴골밀도에 부정적 영향을 미치지 않는 것으로 나타났다.

• Journal of Nutrition and Health
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• 제29권9호
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• pp.950-957
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• 1996

This study was designed to investigate the effects of caffeine intake on Ca and P metabolism in ovariectomized rats. For this purpose, the ovariectomized female rats weighing 230.8$\pm$3.5g were divided into four groups, eight rats each ; control, low caffeine(LC), medium caffeine(MC)and high caffeine(HC) groups. They were supplied for six weeks with the caffeine of 0mg, 66.8mg, 167mg and 334mg per kilogram of diet, respectively, and the results are summarized as follows. 1) There were no significant differences in feed intake, feed efficiency ratio and body weight change among all of experimental groups. But liver weight(both total weight and weight/body weight) was significantly decreased by caffeine in MC group. 2) The weight of tibia was decreased by caffeine intake in MC and HC groups. But the length, Ca and P content of tibia and femur was not changed in all of caffeine groups. 3) Ca, PTH and calcitonin levels in serum were not affected by caffeine. While, serum P level in HC group was significantly increased as compared with the control. 4) The fecal excretion of Ca and P tended to be higher in the caffeine groups, and as the result, the absorption rate, retention and retention rate of Ca and P tended to be decreased. It was noteworthy that P retention was significantly lowered in HC group as compared with LC group. The results showed that, when caffeine was taken by ovarietomized rats, the weight of tibia was decreased and the retention rate of Ca and P tended to be lowered. Therefore, too much intake of caffeine for women whose bone mineral density of tibia is decreased after postmenopause seems to accelerate the decrease of bone mineral density due to the negative effect on metabolism of Ca and P.

• 한국가축번식학회지
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• 제25권4호
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• pp.349-357
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• 2001

본 연구는 caffeine과 다불포화 지방산이 다량으로 함유되어 있는 대두유(soybean oil) 급여 가 생쥐의 유선발달에 미치는 영향을 검토하였다. 생쥐를 0, 5, 20%의 3개 지방급여 구로 나누고 각 구의 반은 caffeine처리를 하였으며 나머지는 무처리 하였다. Caffeine처리시 무처리구에 비하여 시험종료시 체중, 일당 증체량 및 유선의 무게(wet weight)를 감소시키는 것으로 나타났다(P＜0.05). 그러나 caffeine처리시 무처리구에 비하여, 유의하여 제 4유선의 유선발달 score및 DNA함량/gland을 증가시켰다(P＜0.05). 지방급여 수준효과에 있어서는 caffeine처리구와 무처리구 모두에서 지방함량이 증가할수록 유선발달 score 및 DNA함량/gland이 증가하였다(P＜0.05). Caffeine 급여와 20% 지방급여구 간에는 유선발달에 상호작용 효과가 있었다［(20% 지방 + caffeine) - (20% 지방 + no cafferine) vs (0% 지방 + caffeine) - (0% 지방 + no caffeine)］ (P＜0.01). Phosphodiesterase 활성 억제인자인 caffeine을 생쥐에 급여할 시 유선의 발달을 증진시키는 것으로 나타났으며, 지방급여에 따른 유선발달은 다불포화 지방산이 다량으로 함유되어 있는 대두유를 0∼20%로 증가함에 따라 유선발달이 증가하였다. Caffeine과 대두유를 병행하여 급여할 시 유선발달에 상승효과를 가져오는 것으로 나타났다.

• 한국식품영양과학회지
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• 제23권1호
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• pp.13-22
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• 1994

This study was designed to investigate the effects of dietary protein and caffeine consumption levels on Ca, P, Na and K metabolism. One hundred twenty rats were divided into twelve groups according to age, protein level and caffeine consumption such as group(120-130g young rat, 250-300g adult rat) , dietary protein group (20% normal protein , 85 low protein) , caffeine consumption group(0, 3.5mg, 7.0mg). Low protein diet containing high caffeine levels caffeine levels increased calcium, phosphorus, sodium and potassium contents of urine and fecal in rats. Young rat has higher level of calcium, phosphorus , sodium and potassium than adult rat. In the serum, calcium contents were not affected by age, dietary protein levels and caffeine consumption . However, phosphorus, sodium and potassium contents of serum in adult rat were higher than young rat. In the liver, potassium decreased with decreasing dietary protein levels. In the kidney, calcium , phosphorus and potassium contents were not different by age group, dietary protein levels and caffeine consumption , but sodium content was significantly reduced in the adult rat.

• Preventive Nutrition and Food Science
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• 제15권3호
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• pp.229-232
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• 2010

Caffeine and catechins from the Yabukita, Yutakamidori, Saemidori, Okumidori, and Fushun varieties of tea leaves picked during different harvesting seasons from April to October were evaluated using HPLC. Total content of catechins increased greatly with the later harvesting time of tea leaves (i.e., picking the leaves in September versus in April) and decreased slightly after September. Yabukita tea leaves picked in August contained 43.1 mg% catechins including EGC, EC, ECG, and EGCG, with the ECGC levels constituting greater than 50% of those four compounds. Yutakamidori and Okumidori varieties picked in September contained the highest catechin values, at 43.6 mg% and 31.0 mg%, respectively. Fushun and Saemidori varieties contained lower catechin concentrations of 14.5 mg% (July) and 11.7 mg% (August) compared to other varieties. The EGCG levels gradually decreased in the late harvesting season, while levels of the other catechins, EC, EGC, and ECG, gradually increased. All varieties of green tea showed a gradual decrease in caffeine content toward the end of our harvesting efforts in October, with levels of 58～68 mg% in April and 28～57 mg% in October. Yabukita, Saemidori, and Okumidori varieties reached their highest caffeine levels in late spring/early summer, with Yabukita and Okumidori varieties reaching a high of 73.4% and 63.5% caffeine, respectively, in May, and Saemidori at 64.0% in June. In particular, Fushun still contained high caffeine of 66.8 mg% (September) during the late harvesting season.